FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n02_Tr4_C8I1b_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n02_Tr4_C8I1b_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80
Sequences flagged as poor quality0
Sequence length251
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTGGTTTGCTGGGCGGTGGCGGCAAAGCCCTGGGCGCACAACAGCAGT11.25No Hit
AAATGAACATCCTCCCGCTGCCGCCCACCGTGCCGGCGAGCGCTCGCCCG11.25No Hit
CTTCTCGGTGACGTCGAGAATAATGTCGCGGGTTTCACTGCTATTGATTT11.25No Hit
TCGCTAGACCACTCGCCATGTCCATGACCATGCCGCCTCATCATGTCACC11.25No Hit
CTTTGTAATAGCCGGCCAGATCGAGAATGCCGAAGCCCGGGGTCTTGAAC11.25No Hit
AAGCGCAAGTCGCTAGACGTACTCGTCCTGGTGCTCTCTGAATTGTTCAG11.25No Hit
GAATGGAAGATGCCGGTTACACGCAGGTCAAGGACCTGACGAAAGACGAC11.25No Hit
GAGAAGAAGTATGAGCGGCACGATGGCGGAACGTATTGCGCAAAGATTCG11.25No Hit
AGGGACACGTCGCAGGGGGACGGGGTGACTCTGTGGGGTGGTGTGGTACG11.25No Hit
CCTACCCGGCGCCGAGCCTCAGCCGGAAACTGGCCATCGATGCGCTGCAA11.25No Hit
CCAGAGAGCTATTCCGCATTGCGCGCTATTCGCGTTGCACGAGCGCTCAA11.25No Hit
GTCTCTAAGCACTGCTTGATTGGCCTTGTGACGTCTTGTCGAATTTTTGC11.25No Hit
ATCATATTGTCAGGCCTGACCGCGCTGGTTCTTTTCTGTCTATCGATCCA11.25No Hit
GAGCTGCTGAGCGCTCTGTGCATTGGTTACGAAACCGCACTGCGTGCCGG11.25No Hit
ATATTATTGAAATGGGAGTTATGCTAGTTAATGCACCAATTGCTAACATT11.25No Hit
TCATCCAGGAATAAACGGGAAGCCTGTGTGGGAGCGAGCCTGCTCGCGAA11.25No Hit
GTCTGGATGTGACCGCCGACAACGCCGCCAGCCGCGAGCTGCTCGGTCGC11.25No Hit
CAATCACGCCATACAACTCGTCGCGACGACTGTCGCGGCCGTCATTGCTG11.25No Hit
GTTTGAAATTAAAGTTCGCCGCTTGCCTCCGCTCTTTTTTTCAGTCTATT11.25No Hit
TTTCCCACTACACGCGGCGACGGTGCCCGACGCCTCCCCCCTTATGTCGT11.25No Hit
GCAATGTACGCAGGATCTGCTGTACCGGCAGTCTCGGATCGTTGCGCACG11.25No Hit
TTCTCTCGTTCTCGTGGCACCTCTTCTCGAGACGCTGGCGGTTCCCCCGC11.25No Hit
GCTGCGCACCCCTTCTTCGGCCAGCTCACCTTCATCCGCGTGTACTCCGG11.25No Hit
ATTTTTGTCAGTCCGTAATGGTCCGGCTCAATTTCGCTTACCTTTCCAAT11.25No Hit
CTCCTACTCTTGCATCTGGAAGCTCTTCTCGTTTTTCCCATTCAAGCGTT11.25No Hit
GATCAAGCGCCCGTTCTCGACCACCGGCAAGTGGCGTAGGCGCTTGTCCG11.25No Hit
GGCACCGTCCTGGTCATCACCCTGGGCACCGGAATCGGCTCCGCCTTCAT11.25No Hit
CGCAGAAGGTGTTCGGGACAGGCGCTGGCTCTCACTCTTTTTTGATGGCG11.25No Hit
CCAAAAGAATCTATTTAGCAGCTGCATCCCCCGCTCGGTATCTCATATTT11.25No Hit
CTAGGTATCTCCGCAAGAAGGGCAGCAAGGATTTCAGCCCGTCGCGTTTC11.25No Hit
GTCGTAGTGTGGCGCGGGGCTGCGGTGGTCCGCCCCCCAATCTCTCGGCA11.25No Hit
GCCGAGGAGCGTGGCCTTTTGAATGCCGAGCTTTTCCAGCTGTCTCTTAT11.25No Hit
GGCCTATAATGGCTGCCTTTTTCGCCCAATGCTTTTGCGGAGCTGGTGAT11.25No Hit
ATCCAGGTGGTCTGCCCATTCTCTTCGATACGCAGGCAAGGCACCTTGAT11.25No Hit
GCCTTGCCGTCCTTGTTGCCGTAGAATTTCACGCCCTCGCCGGGTTTGAC11.25No Hit
AGCCATCCGAGTCGAACCAGCCCTTGAGCACGGTAAACAGGTTGAGCAGG11.25No Hit
GTTCAGCCTCCGTTGCTTTCTAGAAAGGTGGCCGATTTGAACGAGGTCAG11.25No Hit
GTAGTAATCGAGGGCGCGATCAGTCGCTTCTTTTGTCTTTTGAGACTCAA11.25No Hit
CGTCATGCCTGCGATGTTCGGCGTTTCGGCACGGCGTACCAACCACAGTT11.25No Hit
GCGCGTCGTCGCTTTCGTCTTCGCCTTCCAGCGCGTGGTACATCTCCTGC11.25No Hit
TCCCACTTCCCAACGGCCCTGCGAGTCAACGCGAATAGGTGCATGGTGAT11.25No Hit
GCTTTAAGGGACGGCTTATTCATGTATCGATCAGTATCACTGCGTTCTGC11.25No Hit
AACATATGAACAGGTATCGAAGCAGCAAGGACAGCTGCTTGAAGATGCAT11.25No Hit
TCACAAGTCCTTTAAACCTAGCCAAATCTTTAAGGTTTCGTGACTTTGTC11.25No Hit
TTCTGCACTACTGCTGCGCCCAGGTCCTAAAGCGAGGAGACGATCCGGTC11.25No Hit
TCCATACGCCGTTTGGCAGCCGCATCAACCGCGCCTCGGGCCTGGCCCTG11.25No Hit
ACACAGCACTCTGCAAACACGAAAGTGGACGTATAGGGTGTGACGCCTGC11.25No Hit
GATGCTATCTGTTATGTGGCACCTCATGATGACACTACCTCGTTTTGACT11.25No Hit
CATTCCCGGGGCGAACCTTGGATCGAGTGACAGCGGAGAGGAAAGCGGCA11.25No Hit
CACCGGCAGAGGCGTGTGGTTGCGCGCGGCATTCTTCCCAAGCTCAGCGG11.25No Hit
CAATAGAAGGAACTCGTTCTGATACAAGCTTAGCTAATTCATTTTCAACT11.25No Hit
GTCCCTGACGACATACCTAAAGAAGTCGTTCAAGCCGCAACCGAGAAGAT11.25No Hit
GTAGATGCTGCGACATATGGCTTCGTGATGACTTGTTTACCATCAGCACT11.25No Hit
GTATTCAAGACAATGGGGCAAACGAGTGGGCAGCGCAGCAGGCGCTGGGC11.25No Hit
ATGGATCGGCGAAACGGATATCGACCTGGTGACCGCTGTTTTGTCTGTCT11.25No Hit
CGACAAACCAATGCACACGGTCACCAGCAAGGCATCGAGGTCCGACAGGT11.25No Hit
GAGGGACACGCTGCTTGATCACTGTGGGCAGGAACGCGAGCCTTGTCTCC11.25No Hit
GTCTTGCTTCACGCAGGACCAGCCCTGCCCACAACCCGACGACCCCGCCG11.25No Hit
ATTTTCGATGAAACCTTCAAAAGTGACGGTACCGTGAGCAACAAGCATCG11.25No Hit
GCCCAAAACACCATCTACGACCCGACCCCAGGACCTGGACACTACACGGG11.25No Hit
CTGTTGCGAGGCGTACAGCGGCGCAAGACCATAAAACGAACCGATGATCA11.25No Hit
ATCAAGGACCGCGCGAGCAACGGCGATCGCGATTTCGCCGCCCTGTTAAA11.25No Hit
TCTGTACTCTGCTAAGGAGGCTAAAGAAAAGGCCTTCGGCCGAAGAGCCA11.25No Hit
GAACACATGCGTTGAACAGTCCGTGGATTCAGGCGGGGCGTCTGGCGTTT11.25No Hit
AGCACCGGGCGGGCTCAGCATGAGCCGGCAGTATCAGCCCACGACCAGGA11.25No Hit
GTCGCAAGCTTTTCGGGCGGCACGTAGAAAGCACCGGATGTGTAGAGGTC11.25No Hit
GTATGGCCTTTGGCGCCGTAAGCGCTAAACTCGGGGTGGCTGTCTTTCTT11.25No Hit
GTCATAGACTCCCCGGCGACCATTAGGCGCAGGGGCGTAGGGTCTGTCTC11.25No Hit
GGTCGGTTCTTCTGCTGATCGTGATCGTTACAATCTGCCCGATCGGATTT11.25No Hit
GGATGTACCTGGGCAGCCAAGGTGACCACGGCACAGAAACACAAAACCAC11.25No Hit
CCTACAGAGGTACGCATTTCAAATTGTAGGAGTGCGCCTGCTCGCGATAG11.25No Hit
GCCTGGTACTGTCCGGTTTCGGCACCATCCTCGGCGCGGTCAACTTCATT11.25No Hit
GTTTCTGGCCGCCGCAGCGCTGCTGATCGCCTTTTCCCGTCCCGCGCGCG11.25No Hit
TCGGCGGGCTGATCGCGCAGATCGGTCTGGTGCTGTTGGGCCGACAGGAT11.25No Hit
GCGTGATAGCTGATCTGGCAGTCTCCTTTCCGACACCTTCAAACCCCGGA11.25No Hit
CGGCTATACCGTGCACTGGCTCGGCACACCACGCGGCATCGAAAACGAAC11.25No Hit
GCGATCTTGCAAGCGATGCAACGGCGCGGTCGGCGCGTCGATCGCCAGGT11.25No Hit
GTTGTATTCATATTCAAAGGTGAGCTGTAAGTTTTTAATTGAGACAACGC11.25No Hit
GTCGCTGTAGGCTCGTGTGGGTGGCAGTGAACGGTGGTCTGCGACCGGCG11.25No Hit
ATTCGTGGGTGGTGGAACTCGGCAGTGAGGAGGCGGTGCCGAACTTGCAG11.25No Hit

[FAIL]Adapter Content

Adapter graph