FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n02_Tr4_C7I1b_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n02_Tr4_C7I1b_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88
Sequences flagged as poor quality0
Sequence length251
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATCAAGGAACTGCTGGATACCCGCGTTCGCCCCGCCGTGGCGCAGGA11.1363636363636365No Hit
CCCTAACAGGCACAACATTTTCCGGTGCGTATTGCCCCTTTCTGCGCTCA11.1363636363636365No Hit
CCACTCGCCTGACGGCATGATGTTCGATAGCACTGGTCTGCTCTGGATCC11.1363636363636365No Hit
GGCATTTTGCTCGGGAATGGAGTCTTCCTCCTTTCCTATTGTCTTTTTCA11.1363636363636365No Hit
AGAGATGGGTGATGACACATTAGAAATTGAACGCATTTATATCAAACAGT11.1363636363636365No Hit
AGGTGGAACCGGGTCCTATCCACCGGGATTCCACGGATGACAGCGACCTA11.1363636363636365No Hit
AATCGGTGCGCCTCCGCTATATGTACTGTCTAAGCGGTTAACGAAAAACG11.1363636363636365No Hit
CGCGAGTTCCCCGCAATCGCCGAAGTACTCGGCGTCGGCTGCTCGATCGA11.1363636363636365No Hit
GTTATCGCCGGTTTGAAAAGGATATGGATACACCGCCTGAAAAAGAAGCG11.1363636363636365No Hit
ATATAGAGCAGATGATCCGCCGCCTCGTCGCGTCGGCCCTGCGCGTTCAG11.1363636363636365No Hit
GTCTAACGGTCATTGAGGCACATATTTTCGTTGGCTGAAACACGAGAAGT11.1363636363636365No Hit
ATATAATGCACAGCCAGCTCTTTTAACGACAAGCATCGCGATCTTAAAGA11.1363636363636365No Hit
ATCGGTAGAAGTGTCCTCATCGCCATCCTGGCGGTACTGGGCATCATCCT11.1363636363636365No Hit
GTGCTAAAGTAGGTACACTAGTATCTTCTACAAATTGACGGCATTCTGAT11.1363636363636365No Hit
ATATTTAAGTCTGTATAAAATTGAGCAAGTCGATCATCAAGATCAGATTT11.1363636363636365No Hit
AGCTGGAAGAGGATTTCGACGGGGCGGGCGCGGATGTGCTGATGATCGGC11.1363636363636365No Hit
TCTTAGTGCGTTCAGCACAACCGATACAGAACTAAAGGCCATTGCCGCTC11.1363636363636365No Hit
CATCACTTCCTTGGAATAGGTTTAGCTGCTGGCGAAAAAGAGGCAGGTGA11.1363636363636365No Hit
GAACGAGTGTCGGAACGCTGATGTTCTTCAGGTCGTCGGTCTGATCGGTT11.1363636363636365No Hit
ATGCAGATTCGCCATCGAGCAAAATAGTGCAAGTACATCCAGTTTGCAAA11.1363636363636365No Hit
CCCGGACCTGGCTGCGCAAATCCGGCGTGCTCAATGAATTCTCCTGTGAT11.1363636363636365No Hit
CTTTAGGAGTTTCCGTGTGCCAATCAAAAGCTCATGTCCTGCAGCTCTTG11.1363636363636365No Hit
GCGGATCAGGTCCGGCATGACGGAGGAGAAAACTGCGAGGAAACCAACAC11.1363636363636365No Hit
GGGGGCAAGCGGCCCTTGCAAAGGCTGTGCGCTTGCGAAGGCCGATGCCC11.1363636363636365No Hit
GGTGAAAAGGCCAGCGCCATCGCCACCATCCACCGCTTCATGAAGCTACA11.1363636363636365No Hit
ATACATAGCAGGTATAGTTCTTGGGGTTATTTTTAACAATATTCTACCTA11.1363636363636365No Hit
GTCGGCCGTTTTCCCCATAAGAAAAATCTTCGTGTTTCCTAGCGGATCGG11.1363636363636365No Hit
CAAGCAGCGTAGGACCTATTCCTGTGTGAGACGTTGGTTAGTGCAATTCA11.1363636363636365No Hit
GGATACCGTCCAGCGTCTGGACCGCGCCGCCCCGACCAGCGTTGCGATGA11.1363636363636365No Hit
CAATAAGGCACATATGTTTCGAGGCAAATTAAGTGCCATGACACTGGCGG11.1363636363636365No Hit
CTCTAAAATCAATTTTTGCTTTTCAGGCAGCCCGATATTCATAATGTAAA11.1363636363636365No Hit
TCCTAGAGCCTTGTTTCGCCGCATCTGGGGCTTTGACGACCCTCGCGGGC11.1363636363636365No Hit
GAAGAAACTATTGAAGCTTTTTGAAATACAACTGAATCATCCTACAAACG11.1363636363636365No Hit
ATCGGCGGGAAGCCTATCGAGCATCGGTGGCTGCTCCCCCTTGTAGAGCT11.1363636363636365No Hit
GATTGCAGTCCTACTCGACAGAAAGAAATCTGCTTAAGGAGGCAGCTGCT11.1363636363636365No Hit
TATGAAGTGCTTCAGCTGAGGCATCCTTTTGCTTTACATACTCCTCTAGA11.1363636363636365No Hit
GGGTTATTCAGCAAGTCTCATTAGTGTTATCATGACCGTCTTTAACCTTG11.1363636363636365No Hit
GTCGTAGCCCACCTTCACCGAATATTCGCCGGGATGCAGGGTTTCCTGAT11.1363636363636365No Hit
CAAGAATTAATTCTAGACGTGTAACGCAAAATAGTATAGGGTTTGATCAA11.1363636363636365No Hit
CCTCACAAGCGTTGATTATTCGTAAGTATCATTATAGCATACAACATCTG11.1363636363636365No Hit
CTGGCGTCCTTCGCCACCTTCTCCATCGCCTTTTTTGCCCGTCCGCTGGG11.1363636363636365No Hit
GGGACCACGTATCTTTCCCAGCCTTACCCACACCGCCCCCACCGCCGCCC11.1363636363636365No Hit
CGAAAATTGCCGGGCGCTTTTAGCGGTGAAGCGCAGCGCATAATCTTCCC11.1363636363636365No Hit
GAAGAGGACATTTCATGTATCTCTATTGGGTTCATGCATAGCATAATTAC11.1363636363636365No Hit
GTATTTTTTTATTCTCTCTCCACCCGTCGTTCGCCTGCCCCCCACCGCCC11.1363636363636365No Hit
TTCCTGGTTCGTGCCGTCCTCTTTGTGGATTTTCAGAGAGGCAGTTTTAC11.1363636363636365No Hit
GTTTGGGACCGAGTGTAGCCGCGCACGCCCCGAAGATTAGCAGCAGCGCC11.1363636363636365No Hit
GGCCCTTACACACTGCGTGAAATCACCATGAACCGTGCGTTTCTTTACAT11.1363636363636365No Hit
GTATGGAAAGCGGCGTAAGATGGGTTCAACAAAAGCAAACAGACACGCAG11.1363636363636365No Hit
ACATACTGGTCCATCTGGGGATCGTTGCTGATTATCGCGGTGACCTATTT11.1363636363636365No Hit
GCACAATGCGGGGAGGGTAAACTGCCGGCGATTATACATCAGAGGTTTAC11.1363636363636365No Hit
GATATCCAGATGCCCGTAATGGACGGCGTCGAGGCGACCCTGTCTCTTAT11.1363636363636365No Hit
GTAGTGCTACAAGAATAGATGATGAATGAGAGCTAGATAAAATGGCTATT11.1363636363636365No Hit
TTTGTTGTTTCCCATTCTCCCTCACCCTAGACCTATTCTTTAAAACCTCT11.1363636363636365No Hit
TTTATAATCCGCTCACACAACAAATAAAAAAAGACTACTTCTTGCTGTTC11.1363636363636365No Hit
TTCCCGAACTATCCGCGAGTGTTTTCGGCTGATAGCTCCGCCCGAACCCG11.1363636363636365No Hit
GATCAGCAGAAAGCTCGGCGCGTGACGCAGGCGCAGGTTTTCCGCGTCGG11.1363636363636365No Hit
AAACGAACAGTACAAGAAGCAATGCGCCTCCGCCTGCACAAGCGCCACCG11.1363636363636365No Hit
GCACTATATTCTCGCCATGACCTTCCTCAGCCTGGCGCCTGTCACCGCCG11.1363636363636365No Hit
GCTGGATCCATGCCGATGATTTCCATGGGGCCAAGTATTATGAAGAGCTC11.1363636363636365No Hit
TCGTCAGGCGGCCGGTGGTTACGCCATGGGTCCAGAGCAGCGGCATGCGA11.1363636363636365No Hit
CATGAGCAATGAGCCGGCGTCCGATTCCACGGCCCTGACGGTTTGGAGAA11.1363636363636365No Hit
TTCTATAACTTCATTCTATTCAATAGCGTACCACATTTTACGCCAAAAGA11.1363636363636365No Hit
CGGTGTTAACTGAATTATTTTTAAGGGCAGAAGAGCGAATTCACGCTCTC11.1363636363636365No Hit
ACCAAGGGCAGCGAAGCGCCAGCGCCCCAGGCCCTACCGGCGCTGGTGCA11.1363636363636365No Hit
GTCGTAGTTTCGGTGGGGAGGGAGAGGTGGATGAGAAAGAGGGATTAATT11.1363636363636365No Hit
AAATCATGCTTCGCGCGGATCGAGCGCGTCGCGCAGGCCGTCGCCCAGAC11.1363636363636365No Hit
GCTCAATCGTGCCATTACCTCCCGCTCACCCGACGCTCTAACGTGCACGC11.1363636363636365No Hit
CTCTTTACTCTTTTTCGTTTTCCTCTTACAATTACATTTACTGGATAACT11.1363636363636365No Hit
GTTTCCGGGCGTGAAAGCGCTTGAGAACGTCAACCTCAAGGTTAAGGAGG11.1363636363636365No Hit
TTTTTTGTGATTTCGCCATTTAATTTAAAACTTGCTATTGACGGACAAGC11.1363636363636365No Hit
CATTATAGCTCCAGCTCTTTTTCAATTTCTTTTTTTGAGAGCATTTGTTG11.1363636363636365No Hit
CGTTTAACGGAAAAACGCTCCGCTCAGCACAAGGGTCTCATTTTCACCTG11.1363636363636365No Hit
TTTATATGGCGGATTATATCCAGCGTTAGTCATTACAGGGATGCACCATA11.1363636363636365No Hit
CAGTTGTTTTAACAGCTTCACAAGCTGTCCATAATTGTACCGGTCAAAGT11.1363636363636365No Hit
AGTGTCGGCAAAACCGCATCAGGACGTTCTTTTCGAATGATTCGTGTTAA11.1363636363636365No Hit
GTGATAACTTTTAAGATTTTCGGGCCGTTTTCACGGGTATACGAAATAAA11.1363636363636365No Hit
TCCAGATGTAGGCGAGGATCCCCGAAACCGGGTGGAAGATCAGGTTCTTC11.1363636363636365No Hit
CGCTCATAGACGCCCGTATCGACGCTGACTTCAAAGACCCGCACTTTCGG11.1363636363636365No Hit
GATTGCAATACAGGGTGACCTGCACTTCGCCGTGCGCGGCTTTGCTAATG11.1363636363636365No Hit
GTTGTACCAGGTAATGGTGTTAAGCCAGTCATTGCCGACGGTTCTGTCAA11.1363636363636365No Hit
GCCCGCATCCTCCCCGCGAACCTCCTCGCACCTTTCGTCGAGCGGGCTTC11.1363636363636365No Hit
CGCACGGGCAGTGTCGCGGCACTGACGGACAGCAATGCGGTGAAAGACAG11.1363636363636365No Hit
CCAGGGAAAGCCAGAATCCGCTACTCTGTGAAATCGAGAAGGACGGTTAA11.1363636363636365No Hit
GTGCTGACCTTGCACAGCAAATTGCCAGTGCGGAAAGCAGCCCAGAAAAA11.1363636363636365No Hit
GGGAGTGGGAGGAGGTGATGGGACTGCTTTCGGAGACGGAATGCATAGAC11.1363636363636365No Hit
CCTTTCCATCGCCGTTGTTCACTTTTAATACCGCACTGCACTGCCTCCTA11.1363636363636365No Hit
GAATTACGGAATCGACATTCCATTAGAAGAATAAATCATATGACATAGAT11.1363636363636365No Hit

[FAIL]Adapter Content

Adapter graph