FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n02_Tr4_C5I4c_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n02_Tr4_C5I4c_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98
Sequences flagged as poor quality0
Sequence length251
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAGGATTTTTATTGCCTCCAATTCGAGAAGCATTCAGCGCTCTTCATA11.0204081632653061No Hit
GTTCAGGGGCATGGTTCACCCATGAAAGAAAAAGCCCGCTACCACAAGGA11.0204081632653061No Hit
CCCCGGAATGTGGCAAGTGCAAATTCTGCCTGTCGGGCAAGACCAACCTG11.0204081632653061No Hit
GGAGTCAAGTGAATGTTAAGAGAAAAATTGAAACTGCAATTGATTTTACT11.0204081632653061No Hit
ACTGGATGCGAGCCGAAGGCACCCGCTGCCTCATCTCCGGCATGGCCGGC11.0204081632653061No Hit
CCGCAAAAGGTCAATGGCGAAGAACGCATTCGCTACAGCGGCTCGCCGAT11.0204081632653061No Hit
TCTCCTGGTGCCGCGACTGCGCCAGCTCGAGCAGGGCGACGACACGGCCG11.0204081632653061No Hit
GTCAACAAATCTCTCAATGTCCTTGAGCAAAATATCGTTCGCCTGTTTTA11.0204081632653061No Hit
GAGTTCCTCACCGGCCTGGGCATCGGCCGCTACGGCGTGCTCGCGCTCAT11.0204081632653061No Hit
GTATTTGATTTTTGATCCTTATAATTCTTCGTTTCGTATATTTTGTTCAC11.0204081632653061No Hit
TTTTTTGCGTGCAGGTGAAGTCATAAAAAGACAATTGTATAAAGATTAGT11.0204081632653061No Hit
ATGTACCCTGTTGAATAGCCTCGCGCAAACCAGCCATCAGGACTTGGTAG11.0204081632653061No Hit
CCTTAGAACATGTCCACCAATTTCTCGGTTCATTTCATTTGAATCAAGCT11.0204081632653061No Hit
ACGATACATTCCTTGATCGGAACAAGTTTATGAGAATTCAGCCCATAAAG11.0204081632653061No Hit
CGCCGATATGCGCTTCGCTCTGCCCGGAAATTTCCGCGCTGAGCTTGCGC11.0204081632653061No Hit
ATCCATGGGATGTCCCTATCGATTGGAAAGTGTGGTGCCATGGTAGCGGA11.0204081632653061No Hit
GTACTATGATGAGCGCCCTATTTCCTTTCAGACGGATATTTTTCAAAAGG11.0204081632653061No Hit
TCCATATTATTTATGCCTCGCTTGGCGTAGGCATCCCGCTAATGATTTTG11.0204081632653061No Hit
CCGTTGAGATGATCAAGACGCCAGACCTGGTTCCCTTCATGCACAGCACG11.0204081632653061No Hit
GTGTAGAGCTCGTCGCCCTCGAAGTCCGTCGCCCAGAGCAGCTTCGTCGC11.0204081632653061No Hit
GGGTCGGGGCGGTGTGGTCGCCTTTCACGGCGCCTTGGGGAACCGGGATT11.0204081632653061No Hit
GTTTTCTTGATTTAGCTTATCAAAAAGAGTTACTCGATTTGATTAAACAA11.0204081632653061No Hit
CATCTATGCGGGCCTCAGCCGCGGCGGCATCAACCAGAAGTACCTCGCGC11.0204081632653061No Hit
AAATGGCACCCCTTGTCCTTGAGCTAAAAAAACATCCACAAATTAAATCT11.0204081632653061No Hit
TTTCTAACCATAAAAACAGGGGTGTAGCTGTTGCAATACATAGGTACGCA11.0204081632653061No Hit
GGCTGCCCGTGACTCACGGCGAATACTGGACCAAGAACAAGGATCAGGTC11.0204081632653061No Hit
TACTCATACGTGACGGGTTACAGCAACTCCAAAAAGTAAAAACCCCCGAG11.0204081632653061No Hit
TACTAATATTAAGTAAACTTCATGTTGGCCAACACCAGGATGTATTCTAT11.0204081632653061No Hit
TATCCGGGGTCAGGATGTAACGACCAATGATTGCCAGGTTGGAAGGTGCA11.0204081632653061No Hit
CTGTAATTACAGCTGGATGCACCTATACCAAGGGAGCGTTTAAGAGCGGT11.0204081632653061No Hit
GCTGTACCTGGTGATACCGGGTTCGGTGATCGGTTTCACGGCGTACCTGA11.0204081632653061No Hit
GCTCATGAGCGTTCCTCTGTGGGGCTTTCTTATTGTTCTGGTCACAGGGT11.0204081632653061No Hit
GTCTAAAATCGCTGCTCCAGCTGCTGTTAACACACCAGCTTCTTCGCCTG11.0204081632653061No Hit
CTTTCACTCTTCTTCAACGAACGCGGATGTGGCAGCACCGATGAGTAGAC11.0204081632653061No Hit
TATATGTATAGCGTTCAGAAATGGTTTGAATTTGCACCTCGTCTAGTGAG11.0204081632653061No Hit
GCTGAGCGCAGCATCCTCACGCTGAGCGCAGCACGCGTCACGCTGAGCGC11.0204081632653061No Hit
CCGCAAATTCGTAATCATCATAAGCTTTTTTCACTTTATCAATCAATTTG11.0204081632653061No Hit
CTAACTTAGCCAGGTTTGTGCAAGTCACCCGGGAAACACTCTGTTAAGCT11.0204081632653061No Hit
ATTATACGGATAACGGGGAAGATGCATTAGTCATGTGGGTGAAGATAAAT11.0204081632653061No Hit
AGTAACCGGTATTGATGCTTCTAACTTAAAAGACAAACTAGACAAACAAA11.0204081632653061No Hit
TATGAAACCCCTGAGAGCACCAGCCGTCTGCCGAGCCTTCGATCATACAA11.0204081632653061No Hit
TGAGATCCCTGCTCGGCCAGCTCCGCGACGGCCTGGGCCATGCCGTGCAT11.0204081632653061No Hit
AGTGGGACCATTCCGCCAAATCCCGCTGAGCTGCTTTCCTCCTCTGTGAT11.0204081632653061No Hit
CATTTGAGGGAGAAATGAAAGAAGCCGGCATTTGGCGTCCAAAAATATTT11.0204081632653061No Hit
TCAAACACGCCTCACCTCCGCCCACCGCCTTGCTATTGTCCGTCAGTTGC11.0204081632653061No Hit
ACCTTTCTCCATATGGAGGAAGGTCATTTCTTTATTTCGTCTAACAGTTC11.0204081632653061No Hit
GTGCCAGGGTGCTGCCCAAGGATGTGCGCGACGAACCCGGCAGCGGTGCG11.0204081632653061No Hit
GCGCTGCACTGCGTCGCCGGCGAACAGCGAGCGCTCGGGCGCGGAGATGG11.0204081632653061No Hit
CTTCTAATGATGTGGTACGTTTTACGATTGTTGCTGTTATCGACAACGTA11.0204081632653061No Hit
AACCTATGCGCTACCTCGGCCAGCCGCTCAATGGCCTGTTCTGCCTGGCC11.0204081632653061No Hit
ACTTCTTCTTTTCTGAAAAGCGTCATCAGTTTCTCTAACATCACGAGCTG11.0204081632653061No Hit
CAATGCTCGAGCGCGGCCTCAAGCGCCTGGAGCAGGTCGCCAGCAACGCC11.0204081632653061No Hit
GAATGAACACAATGGGGACAAGGAATGTGTGTCCCTTACACTGCGCGGCC11.0204081632653061No Hit
TCGGTGCCACACCGGAAGGAGCAAAACCCCACGCTGACACAGCTCCATCT11.0204081632653061No Hit
ATATCATGATTTCTCCATACTACAGCTAACGTATGTCCATCTGGGCATAC11.0204081632653061No Hit
CTCCAATCTTGCTGGGCCATGGCGGTGCACGGCGGGATGTCGCAAGCGGG11.0204081632653061No Hit
AGCTAACCTTCGTTGATAATCTAGAAACAGTGAAATAGCTGTCGGCCTTT11.0204081632653061No Hit
GGGTTGTACGATGCGGCGAAGTTCATATTTTTTAAAAACTGTATGTCTCT11.0204081632653061No Hit
ATGTCGATGCGCTGAATCGCCTGCTCAAGCAGACCTTCGAACCGCTGCGG11.0204081632653061No Hit
ACTTAATACAAGCTGAGATGCTTCTGTCTCGTTGACAAGCTCTTCTTCGT11.0204081632653061No Hit
CTATGAGGTTGGCGCGAAGTCAGAAGTTCTATTGATTTTCTGAACTGTCG11.0204081632653061No Hit
TAATTGATGAGCAAGGTCATACTAACCTACTCAACAAAGCAGCACCGCAA11.0204081632653061No Hit
TGTCAAACCAATGGATGCTCCTTCTGCCGTTTTCGCTTGAACAAAGGATG11.0204081632653061No Hit
CCTATATCGCATCAAAAGCAGCATTGATCGCCCACGCCCGAACCCTCGCC11.0204081632653061No Hit
CACCTGCACCGTGCGGTTCTTGCTCGCACCGGGTGGCAGTTGAATGTCGG11.0204081632653061No Hit
GAAAAATTACCTTTTAATAGTAGATTCCTCCTTTATTTCTAAAAAGTTAG11.0204081632653061No Hit
TATTATAACATGATAAAAAATAATGAAAATGATACGACTTGTATACCTAA11.0204081632653061No Hit
GCACTTCGATTTTCGAGGGCGGTGATTGTCGCGTGAATCGCCTGCTGCGG11.0204081632653061No Hit
GTATCCTGCTTATTGGGATTTTCTGGTCCTATCCACATTTCCCGGGGAAT11.0204081632653061No Hit
GGTTTTTAATGGATTCAATTAATGTTAAGTTTCTCAAACCTAAAAAATCC11.0204081632653061No Hit
TTGTAGACGAGTAGTCGGCAAGTAACCCGTTGGCCGCACGCCATGTGTGT11.0204081632653061No Hit
CTTCATCCCGGCACTGAAACACCCATTGCAGCGTTTCACCCGGTGCCAAC11.0204081632653061No Hit
CCCCAGCCTGTGGCGGCAGGCACAGCTGAACGCCATGCACGGGCTGTTCC11.0204081632653061No Hit
TCTCAAAATGCTCATACAGTTCGTCCTGGTTTTTCTTTTTAAAGTCTGCA11.0204081632653061No Hit
GATAAGTGGTGCTTAAGTAATACTGCGATTGACATTGATAAAAACCTAAA11.0204081632653061No Hit
AAAAGTAGTAGCTGACGGAGGCTCTTATTTACATCCGAAAGTCACTCATA11.0204081632653061No Hit
ATATATTTTTGATATGAAAAACCCTAATATTCAGCGTGTCACAAGACATT11.0204081632653061No Hit
AGCGTGTATCCATTGCGCGGAAGAACACACCATGACCCACTCAACCGAAA11.0204081632653061No Hit
ATCGGTGCCGGTGGACGCCTGGGGCTCAATGGCCTGGTATGCAAGGCACC11.0204081632653061No Hit
ACTCAATTCAACAGCCTGAAGAGCTTTAAGCACACCCAATCTAACATCAC11.0204081632653061No Hit
ATCGGTGCCAGCAACGGCGTCATCCTGAGCGGGACGGCAGAGGCCGGCAG11.0204081632653061No Hit
CCGTCAGGGGAGGCGTGAAAGGCCTTGGCGAATTTCTCTTCGGAGGTCTG11.0204081632653061No Hit
GGCCACGGCGAAGTGCATGGCTTGGCCGAAGGCCTGCGGGTCTGGTACGG11.0204081632653061No Hit
TCATAGGACACTTCATCGTCATCAGTGATCACTTCCTTAAAACCGATGGT11.0204081632653061No Hit
TGCCTCATCTCTCCCTTCCTCCTCCGGTCCGCCGCCCCCCTTCATCTCTT11.0204081632653061No Hit
GTGTGGCATGGTCGCTCAGTTGCGTCATCGCCAACCCGGCATAGCCACAC11.0204081632653061No Hit
TACCGACACCGCCAGTTCCACTTCGATGACTTTGCGCAGTTGGTACACCT11.0204081632653061No Hit
CTCTTTCCATCAGCATTTATGGGTTAGCTGTGCTGATTGGAGCTCCGCTG11.0204081632653061No Hit
GTGTAGTTAGTGTATTTTATTTTTTCCGAGTTCGCATCAAGCCACAGAGT11.0204081632653061No Hit
GCTTTTCAGCTTCACCACGGCATCCTGAACCGTATAATGCTGCGGAATCC11.0204081632653061No Hit
GATTTCTCTGAACATTTCCTTAAAGGCTTCATGGTCGATGTTCAACATGT11.0204081632653061No Hit
GAATAGAACGAGGGGCCTGATACCTTTGCCCCTGAAGTGTCACACTCTCT11.0204081632653061No Hit
AACTAATATAAATTGAATCCCACTTGTTAAAATTTGCATCATTTTTGTCG11.0204081632653061No Hit
TTCAAATAGCGTAATGCTCATATGATTAATTAATGAACCATCTGATAATT11.0204081632653061No Hit
ATTATTGACTTAAAAAACCAAGAAAAATTAGCCTTTGTTGCGGATTTAAT11.0204081632653061No Hit
AATATGGCCAGAAGCCTCCACCATACCGCCAGTTTCTTTCCAATCAAACA11.0204081632653061No Hit
TTTCTCGTGTGCGTCCTCTGTTAACAGATATTGAGATTGAATACTCTGGG11.0204081632653061No Hit
ATATTACAGAATCTGGACGAGTCCCTCTACCAACATATTATTTAAGCATT11.0204081632653061No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)