FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n02_Tr4_C5I3c_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n02_Tr4_C5I3c_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42
Sequences flagged as poor quality0
Sequence length251
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAATGGCCTGCTTGGGCCTCGAAGTGACGGAACATCAAGAGGTGGCCGA12.380952380952381No Hit
CTATTTCTACTGTCTTCTACGGACCGTTTTCATCGGCTAAGCTTTAGTTG12.380952380952381No Hit
TTGTTATAATTGTTTTAAGATCTAATTGTTCAAAAAAATTATCCACAACA12.380952380952381No Hit
CCAACAGGTCACGCGGGCCGGCGACAGGCGCTGGCAGTTCGATGTCTTGC12.380952380952381No Hit
GTCCTCTGAAACAAAAAGTTACTGGCGATTATACGGGCCAACTGCAGCTG12.380952380952381No Hit
TCATAATCGACATATTCGATCGATCAGGTTGCACGTTGCCATCGTGAGCC12.380952380952381No Hit
AGCTTCTTGAGCAATCCATTGATGAAGTATTGAATATTTCTGTCCCGATT12.380952380952381No Hit
CTTCAGGCACGGCGCGATACCGCCGACCCCGGCCACGCCGGCGCGCAGGA12.380952380952381No Hit
ATGCTATCTTTTGGATTTGTTCAGAAGTAACTACGCTATTTTCTTTTTTC12.380952380952381No Hit
CGCCCGAGCAGTACCAGGCCAGCGCCGCGCGCCTGGGCAACAAGCTCTTC12.380952380952381No Hit
CTCACTCAATTTTTCTAGCTCACTTCATCTGGCTGCCCCCTGCTTCCCCA12.380952380952381No Hit
AAGTAAAACAGAAGCTACACAAGAACGAATTATGACACTAGCTACAGGAG12.380952380952381No Hit
GGCCGCCGCACATCTGGATGAATCGATCGGCCAGCGTCGACTTGCCATGG12.380952380952381No Hit
CATCTCGCTCGGCGTGGTCGAGAACGTCACCTTTGACGAGGTGGACCCGA12.380952380952381No Hit
GTGCCGCACCATCTCTTCTGTTAACACATCATTTGGCCGTCCTTCGCGGA12.380952380952381No Hit
GTCGATCTTTATTTCGTCGAAGGGGATCTCGGCCAGGGTGGTGAACGTGG12.380952380952381No Hit
ACCCAGCGTTCTTTTTCTTTCCCCCTTACCTCGCCCTTCCGTTCCTCGGC12.380952380952381No Hit
GTAACATATCAATATGAACTTTACCTGGCTGGTAGTTAACAATTGGTTTA12.380952380952381No Hit
AAATGAACCTTGTACCCTTGGCGCCACCCAGCCGACAGCATATTGATACG12.380952380952381No Hit
AGTCATCGATTTCTTCCACAAATACTTTCTGTACATCTTCTTGAGATTGA12.380952380952381No Hit
CATCAAAACGAACCGCCTTTTGCACTTCCGACAAGACCTCACTTTGACGA12.380952380952381No Hit
GTCTGGCGGAAGCCATGGCGGCGGTAGCGGCGATACACGGCGTGAGCGGG12.380952380952381No Hit
GGCTCCCCCTGCGCCACCGCCGGGTCCCGCGCACCACAGTCCCCCTCGTG12.380952380952381No Hit
CCGCAAACTTTGTCTGAGGACATCAAAGCGAAAATCGCAGAATACACGAT12.380952380952381No Hit
TTTCATACCTTTTTTGCTGTCATCCTCTGCCCTCAAGCTATCTTCCAGTG12.380952380952381No Hit
GGCTGGCGATGCGCGCGGCAAACCCGCCGGCGCGGGCCGAGAGGTTTTCC12.380952380952381No Hit
GCTATACAAAGAGGATCATAAAAGGGATGTTGTCTTGGGCTTCTTGGAAT12.380952380952381No Hit
TCCTACAATTCTACATAAAAAGACATCATCTTTCAATATGATTCGCAAAT12.380952380952381No Hit
TACTGATAGTACTATTCTTGGGTCACGGATTTGAATCGACCAAGGAGTGA12.380952380952381No Hit
GGTGAAAGTTTGGTTATGATAAGCTACTCCTTGCGACACCCATAAGCCTT12.380952380952381No Hit
GTGCAGGCCCGGTGCCAGGTCGGCGGGCAACACGGTTTCCACCAGCAGGC12.380952380952381No Hit
TTTCCGTAATTGCCAATGTTCCAGGCGCCAATGGACCGCTTGACCTGCAC12.380952380952381No Hit
ACTCAGGCATCCCGTGATCAGCACCCAGCCGCCGTTACACGGGCGCCCGC12.380952380952381No Hit
GAGATCTAGGCTTCTGGTCTTGACCGGCGCCTCGTTGTCGTGGTGGGCGC12.380952380952381No Hit
TGCTTAAGAAAGATATGTGCCCATAAAAGAGGAAGCTTAAAATGAAAACA12.380952380952381No Hit
GTATAATTTAAGGCTGCACACAAATGCAGATGGGCAGGTAGCTCAGTTGG12.380952380952381No Hit
GAAGAATGACGTGCAGAATAATCAATTTAATAAAAACAAAAAAAACAACA12.380952380952381No Hit
ATATTATATAAAGTACCTGCTGCATCATCCTGTCTTAATTGAACAACAGC12.380952380952381No Hit
AACGGAAAGTGATGCCATGGGCTGAGCAACAGACGGGCTGTTCCCCAGAA12.380952380952381No Hit
GGGTGGTATTTCAAGGATGGCTCCACGCGAACTGGCGTCCACGCTTCAAA12.380952380952381No Hit
CGGCCAGATCCACATGGGCCACGCGGTGAACAAGATCCTCAAGGACATGA12.380952380952381No Hit
AGGTGATCCGCCGCGCCTACCCGCCGGTGCACGGCTGCGGCGACGCGCTT12.380952380952381No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)