FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n02_Tr4_C5I2c_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n02_Tr4_C5I2c_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126
Sequences flagged as poor quality0
Sequence length251
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACACCATACACCTGAAAAAAGGGCTTCACCCATGCCTGGCCCAGGTTG10.7936507936507936No Hit
AGCGGTAAGGGTATTAAGTTCCGTCAGTGTTCTATTCACTTTATCGATTA10.7936507936507936No Hit
ATATGGAGTAAGGGGGAATTAGAACATGAAACAGAAAGCGTTTGCTTCAA10.7936507936507936No Hit
GCAGCGCACCATGGCCAAGGCTGAAGGGCAGGCGGACCGCGTGCTGGAAA10.7936507936507936No Hit
GCCATACAGCGAACTTGTTCTTTAATACAAAATTGATGATCGAATTCATT10.7936507936507936No Hit
CTCGGTGGAGGCGCTGATCCGAATATCAATGTCCGGATGCGCTTCGCGAA10.7936507936507936No Hit
GCGGGAGACTGGGCAAACCGGTTGCCGGCTTTTTAATATTCCCTCAATTC10.7936507936507936No Hit
CCCCGTGGGGGAGGCGCGGGCCGCCTTGGGAGCGGCCCGGCGGCCCCCGT10.7936507936507936No Hit
CTAAGCCAGCGTCGGGGGGACGGTGTGGAAACGCCTCACCGCCTGGAACT10.7936507936507936No Hit
CGTTGGCGTCGTCGCGTATGCCTGTCTTTTCGATACCGCCCTTTCTGGCC10.7936507936507936No Hit
GCTCTTGATTGTGTGGCTGGTCACACACTACCTGGAAACGTCGTACACTA10.7936507936507936No Hit
GGTTTCAACTTTGCTTCTATCTAAATCATCTTTTACATCAATGCCGACGA10.7936507936507936No Hit
ATCAAAATCAAATCTCCATCTCACTTTTCCGTCAGCAGACTGCCTACCAA10.7936507936507936No Hit
GCCATCGTCATTCGCCCGCTGTCCCTGCGCCCCAAGTTGCGCCTGACCGC10.7936507936507936No Hit
CCACGGCACCGAGCTCCGCACGGGTCGCACCGAACTCGTGCATTTACCGC10.7936507936507936No Hit
CTCGACGCACACCGTCGTCGGCTTTGCCTCGCACGCGGTGACGCCGCCTC10.7936507936507936No Hit
ACCAGATTTCCTGCAGGGGCTTCTGCAGATTCACGATCAACTGGCTGCCG10.7936507936507936No Hit
CTACGCCAGCCTCGTTGGCCGCGCCTTGCCGTTCAGGAATGGGCCGTTCA10.7936507936507936No Hit
ATGCCGCCCACCACCAAAGAGATCACGGCAATGAGCGACAGCAGCAGCGT10.7936507936507936No Hit
GTTCACAGGTATACACGGCTTATCGAAAACTGAATCCAGTTGGGGGAAAA10.7936507936507936No Hit
GGTTATGCGTTGCTGCCAGGAAGGATTGCGGCGGTGTACGAGAACCGGGG10.7936507936507936No Hit
TGTATGATGCGCTTAAACCAGGATCTCGATAACCACCCGCAGCATCGACG10.7936507936507936No Hit
TAACATATCAACAGCCGCTGCCATCGTTGCTTCGGCTGCTGGGGCGAAAA10.7936507936507936No Hit
ATTTACTATATGTTCATATTTTTGTCATAAAACATAAGCGGACTGTCACT10.7936507936507936No Hit
GTTCGTGGTCATGCAGTGCCCGCGCACATCATTACGTCCGCTTTAGAGAA10.7936507936507936No Hit
CATCGAGACGCTCCGCCAAGCCGGTATCACCAAGGAAAAATGACCCGACC10.7936507936507936No Hit
ACACCTGTCTGTCCGACGCGCAGCTCGCAGTGCTCAACGCCTCGTACGGG10.7936507936507936No Hit
CTCGAATCCCGTGCCCGAGCCCAGGGGTTGACGACCTTGTTTGTCCTCAC10.7936507936507936No Hit
GTACCGCATGCCCGCTGCGCGGTTGAACACCAGCTTGCCGTCGGCGCCGC10.7936507936507936No Hit
ATCCACTGTGTTGCCGATTCCTTGCTGATCGAGCGCTTTACCAAACATCT10.7936507936507936No Hit
CAACTACACTCAATCCAACAAAATAAAAAAACCAACGTAAAAGATGTTCC10.7936507936507936No Hit
CTTCGAAAGGGCCATGGCGCGTGCCTGTGCGATTGGGAGAAAGACTCAGT10.7936507936507936No Hit
GAAAACTGAGTCTCTTTCAAGGGCCCAGTTTTTTTAGGAGGGAAAGTTGT10.7936507936507936No Hit
GGCCAGTGGAAAGCGCTTGAACGTGTATGCGCCGACTGCGCTGGAGGTCC10.7936507936507936No Hit
GGATGAATATTGCGAGTGGTCGATCACCCGCGACCCCGAAGGCAACATGC10.7936507936507936No Hit
TACATATATTCGTGACGTTCATACAGGAACAAAAGAATTTAGAGAGCTAG10.7936507936507936No Hit
CGTCTGAACCTTGCAATGCTCAAGCAGCGCTATTTCTTGGGCAAACCTGA10.7936507936507936No Hit
TCGTTATTCTTCGCTCCGCAATGGACCGGAGCGCTCGACTCCCCAGTCTT10.7936507936507936No Hit
GTTGGGATCGCTGCGGGGGGGCAGTGTATCGGCGGCTATCCGGTACGTCC10.7936507936507936No Hit
GCTCTGGTACGTCTCAAGGGCGCGGGCAAGTTCGACAGCGAGGACGACGC10.7936507936507936No Hit
CTTTAAACATGTCCATATCGTATGCTTCTTTATAGAAGAACCAGTTTTGT10.7936507936507936No Hit
TGTCTATACAAGTGGTTGCGAGAGAAAACGTAGGTCTTCGAGGACGAAAA10.7936507936507936No Hit
GATGTAAAACGCGTAAGCGGCGAGCATTACCCAGAACACCATGAACAACC10.7936507936507936No Hit
CTTCAATACTGCGACGAGTGCAGCCAAAAAGAAGATCACCGAGTAATGCG10.7936507936507936No Hit
GTATGCATCAGGTCAACACTCTGGTGCTGCTGTGATCTGCCGTTACCCAT10.7936507936507936No Hit
GTATTTATGAATCTGTCCGCCTTTTACGCCTGGCACATGGATAAAGAGCG10.7936507936507936No Hit
ACAAATGACAGTTGAAAACCGACATCAAATATATAAACCGGCTGAATGGC10.7936507936507936No Hit
GTCTTAAGTTGCTCGTGCATGCTGTATCTATTTCCAGAGACTCCCAGTAT10.7936507936507936No Hit
CTACAATGAAGGCTCTTCCTTATCTCCGTTAACGGTGTATGACGCATCAT10.7936507936507936No Hit
GGCCTCTTCTTTTCGGCTTTCAACCGTTCCGTTTGAAGATAATTCAGCCT10.7936507936507936No Hit
GGATGCAGGAATTTCATGCGGGTCACGGTCAGCAGCGCCAGGCCAATCAC10.7936507936507936No Hit
AAATAATATCGAAGACCACTGTTGTGCCGAACAGCCTTCTAGCTAAAACG10.7936507936507936No Hit
GTACATATCGCTACAACTACAGCTACATGCAATACTCTTCCAAACAGTAA10.7936507936507936No Hit
GTTATAAAACTCGTTTTCTTTCAAGTCTCCTTGCAAATAAGTATTTAGAG10.7936507936507936No Hit
GCGATAGTCCTGCCAGGCCAGCGACGCCACCAGACCGATGCGCATCAGCA10.7936507936507936No Hit
GTGGAGAACACACAGGGCACGCCGAAGTTGCGCCCCGGCGTACGGGAGAA10.7936507936507936No Hit
GTGCTGAACGATTTCCTTTAATTCTTCAGGATTAAGAGCAAATGAATGAT10.7936507936507936No Hit
CTGATGGAATGAAGGGGCTTCGGCATTTACAGAAGGGCGATAAAATTGGT10.7936507936507936No Hit
GTCATCTACTTTGCCGAAGGCGCTGACGCTTCTAACTGCAGCGTCGATTA10.7936507936507936No Hit
CTCCACTCCCACCTCACCACTCTCAACGCTGTCACCCTGCTGCTCCTGTC10.7936507936507936No Hit
CTCAGGCACTGCCATACGCCCTGCAACCCAGCATCCTGATCATGTTCGGG10.7936507936507936No Hit
CCTAAATGCTCTTTTGGTGTCACATAGCACAGCATGGCGGTTCCGTACCA10.7936507936507936No Hit
GCAGACACCTAGCGTCACTGGCATACCATCGCCCAGCGCTATGCGCTGGA10.7936507936507936No Hit
CCACCCTGCGTGCCGGGGCGGCCAGTGCGCCGCGTTACAGCGGCAGTGAT10.7936507936507936No Hit
GTTCATGGGGGTGCGGAGTTCGTGGCCGATCTTGGCCAGGAACCCGGCCT10.7936507936507936No Hit
ATGCTGTTGAGTTGCACATGGTTGCCGCTGGTGGGGTCGTAGAGTTCGAT10.7936507936507936No Hit
ATTTTGAATCGTCTCCACAAATTGCAAACGATGCTTCACTCCACTAAATG10.7936507936507936No Hit
ACACTGGAAAACGACGTTCCTTTTTCGGACCGGCGTTTCAAGTCACCATT10.7936507936507936No Hit
GAGAGGTGAAGTCTGGAACCGTCGCAAGAACGGTGAAATCTACCCGCAGT10.7936507936507936No Hit
GTCAGAACATCTAGCTCCATTAAGAGTGACTTCCAGCGTTTAATCCCGTC10.7936507936507936No Hit
CATGTGAAAATTGTATTTGCTGGAGAAGGGACAAGTCGGTCGTCCTATGA10.7936507936507936No Hit
ACCTTCACCGGCGTGACCGACACCGCCAGCCTGGCCGACCAACTGAAGTC10.7936507936507936No Hit
GAGACCACGGTGCGTTTGTTCTACACATCTTACTCGGAATACGACCCGCC10.7936507936507936No Hit
ATTTCATACACCGCCGCATCATCGCGCGACGCGTTGCCGACCCACAGTTT10.7936507936507936No Hit
ATCATGATGTTACTTGTGCTTTTGTCTTGTCTGGTTGCGATTTTCCACTT10.7936507936507936No Hit
GTCGGCAAGCTTTTCTTTGAAATGAATGTCATTTTAAAGGGGCACCTAAT10.7936507936507936No Hit
CGGTGACCACCACGAAGACCAGATACAGGGTCATCGCCAGCATGTAGTAC10.7936507936507936No Hit
CCCTAGATCCATGCAGCGCACATTTATCCGCAAGCTGTCAGGGGGAGAGC10.7936507936507936No Hit
TTCAAAAACAAGACGCAGTTCATTTATTACAAGAACTATTTCAGGTGCTC10.7936507936507936No Hit
GAAAAGACCTCCTTCGAGCAAGTATTGGGAAACAAGAGCTTCCATCTATA10.7936507936507936No Hit
CTTTTTATGATAATATAGACCTAGTAATTGATCGGAAAATGGGAATGCAA10.7936507936507936No Hit
GAGTAAAAAGACGAGATGCGCAAAAACTCCGCTCCACATCCAAACCGACT10.7936507936507936No Hit
GACCTCAACGGCGCAGCGTGAGTCCCTGCACGATAATCAACGGATTCCCA10.7936507936507936No Hit
GGATACGACATCCTCTAGCTTGTGCGCTGGTGGAGGGGGGGGTTGGGGTT10.7936507936507936No Hit
GCCTTCCGTCAACCTTTTCGCCATTGGCTCTCTTGCCAAGGCGCAAGGCT10.7936507936507936No Hit
AACAATGACAGCTCTCTTATCCGGCGGCGCTGATATCGCTCTGACCGGAT10.7936507936507936No Hit
GCCATATCTTCCGTATTGCCAGACATACTCGCAAATGCAATTAGTGCTTT10.7936507936507936No Hit
CTAGTATTTTATGGGGCTTTTCTTCCAACAACGTTGGCATCCGATTTCAG10.7936507936507936No Hit
CATTAGAATCGGCCCCAGCCAACCGGTGGACCGCTTGACCGCCGCCATTG10.7936507936507936No Hit
TTGCCATACTAAGCACAATAATGGACGTGGAGGTAATTTGAGTAAGGGGA10.7936507936507936No Hit
GCAGCAGGCGGAGGGTTGCGGGCTGGTAATCACGCAACACTTCCCCGACA10.7936507936507936No Hit
ATCGTCAGCCTGCGCATCAACCCGGCCCACCGCTATGGACCTTTGGGCGG10.7936507936507936No Hit
GAATAAGAGCACGTGGTGGATGCCTTGGCAATGATAGGCGACGAAGGACG10.7936507936507936No Hit
GGTGTAATAAGCATTGCAACAACAAGAATAATACCTACAGTTTGCAGGGA10.7936507936507936No Hit
ACCGCGAACCGATCGCGCGCGAACTGGTGGCGTTCGTGCTGGGCCGCTGC10.7936507936507936No Hit
AATTCACGCTCTCATGACCCATTCAGGGCGGGCCGCTCGAGACGGGAGAG10.7936507936507936No Hit
TCCACTGGTAGCCGGTGACCTGGATATCGCTATCCGACTCACTGCTGTCG10.7936507936507936No Hit
AAACGGATGTAGACTTGATCATTTCAGAAGCCTTCTCAGAGCAGGGAGTT10.7936507936507936No Hit
GAAATGCGCCCGCCGCTGAGCGACATCCAGACCGCCGTCAGCACCGGCTG10.7936507936507936No Hit
GCTTTGAAGGTCCTAAAAATGTCAAAGATAAAAAGCAAGATATCATTACA10.7936507936507936No Hit
AGGTATGTCCTGCTGGAGATAAACTGCTTGTCATGTAAAGACCGAAGAAA10.7936507936507936No Hit
CTCTACGCTGATGGGTTCTGTGGCAATGACGGCGCTCATAGGACGCATCC10.7936507936507936No Hit
GTATCCACGCGCCCGGCAGTCGAGGCGAACCCCGACTCCGTCGCCCGCGC10.7936507936507936No Hit
CAGCTGCCGCAGGAAAGCTCGTGGCGTCGGCTCCTTGTCCCTGTCTCTTA10.7936507936507936No Hit
GCCCTGTATCGCGCCAAGCAGAAAGGCCGCGGTCGTGCGGTCTTTCACGG10.7936507936507936No Hit
TTAGAGGAGGCGCTAAATTGTGCTGTCGCTGTAAGAGCGGTGAAAAATGG10.7936507936507936No Hit
ACTGGCGGGTTAGCTGCTCAGTATTTCCCGTGGGGGTGATCACCGAAAAC10.7936507936507936No Hit
TCACAAAGCAAGGCAGTCAAACATATTACCGTGTCTCAGAAGGAGACACG10.7936507936507936No Hit
CCGGCAATGCCCTGGATTTCGACTGCCAGATCGGCCTGGCGTGGTTTTTG10.7936507936507936No Hit
ATGTAATATGCTCTGTTGCTTTACTTGTTTGTCCTGCGCTTGCGGTCAGT10.7936507936507936No Hit
TTTTCAAGCCGCCCAGTCCCTAATTGGAAACGAAGATTGCAAAGTTTCCT10.7936507936507936No Hit
TTGATAGGCCCCTTAAAAGGTCCGTGGATTACTCGTCAGCGTTATCGCTG10.7936507936507936No Hit
GGCCTGAGCCAAACGCGCCACACCGCCCGCTTCGGCGTGGCCTGGTAACA10.7936507936507936No Hit
TTCCAGCGCCTTCTGCGCATCCAGCACCTGCTGGTACAGCGCTTCGGTCT10.7936507936507936No Hit
GGCCAAGCGCTTTGATCTCATCGACGACTTCATTTGCTTTTGCTTCGTTG10.7936507936507936No Hit
CAAATCCCTTACTTAAACGAAGTAAATAAATCAAACAAAGCTTACAACCT10.7936507936507936No Hit
GTAATGATGCCACTAGGCAATTCAGTCGGTTCAATGATCGTACGGGAGAG10.7936507936507936No Hit
AGTTTCCAGACGAGAATATACAACAGAAGGTTGTCCTGCATGCTGCTGTG10.7936507936507936No Hit
GCACCAGCCCGCGCTTGAGCACCACGCTCTTCTTGTTGAAGGCCGCGATG10.7936507936507936No Hit
TTTTTTATGTGAGTAACTGGTGGCATATTTTTCATCAGCTTTCTCACTTT10.7936507936507936No Hit
CTGCTCGTGAGGGCGTGCGGCTGCTCGCTTCAGCCGGATCGCAAAGCCGC10.7936507936507936No Hit
GGTCCGGAGCCCTGGACCAGGCGCAGTTGACTCCCCGCGTCCAATGCGTG10.7936507936507936No Hit
ACCGGCGGCTACGCGCTGCTGTACTTCGAGATCGTGTCTACCGTTGCGCT10.7936507936507936No Hit
CGGTTGGAGCTTTTGTGAGTAAAAACAATACTCAATCTTCATCCACCAAG10.7936507936507936No Hit
GCCAACAACATTGCCAACGCCGACACCCCGAACTACAAGGCTCGGGATTT10.7936507936507936No Hit
ATATGAGATAGAGTTGCAGCTCTAATAAGATGTGTCTCGTTTCTTTTGTT10.7936507936507936No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)