FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n02_Tr4_C5I1b_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n02_Tr4_C5I1b_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66
Sequences flagged as poor quality0
Sequence length251
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGGACCTGACCAGCGCCACCCAGGCGCCGCGCCAAATGGCGGCCGA11.5151515151515151No Hit
GTTTGATCTTCAGGGCGTCCATGAAGTCGATCAGATCTTTGGCCAGCGCC11.5151515151515151No Hit
GTTTATTACCAGGGAAATAACTATATAAGAAGTGACCGGCTACAGAATCA11.5151515151515151No Hit
GGCGCCCAGTAACTTAAACATCTCTGATTCCTTCAGAAGCCTGGATCTGC11.5151515151515151No Hit
GGCTTCGTTCCCGCGCCGGGCACGCTCGTGCAACGCGCGCACGGTGTCGC11.5151515151515151No Hit
TTTAACTATAGGCCACGGACTGCGTGGCCTGCGGACAGCCGATGAGGCGT11.5151515151515151No Hit
TGTTTGTGGCTCCCTTCCCGCCCTACCGTATTCCTTCCTCTTCCCCTTCT11.5151515151515151No Hit
CTATTTTCTCTCTCATTTTATGTCTATTTTCCTAAAAAAAAGCTGATCTT11.5151515151515151No Hit
GTCCCCCGGAGGCTCCAGGAACATCAAGTCGTCGGACTTGGCGCTGATGA11.5151515151515151No Hit
ATCTATTCTTGGCCGTATTTCATTGCTTTTGTTTTTGTTCCTTTTGCATC11.5151515151515151No Hit
CTCCTGGCCCACTCCCCGATACCAGACACAACCATTAACTCATCGCCACT11.5151515151515151No Hit
CAGATACACCCGCTCTTTTCGCAATATCCCTTATGTTAGGCATTTTTTCT11.5151515151515151No Hit
GCCTTAGGTGCAGCCATCAGTGCAGTCAATGTGCTTGAACATTTGATGAT11.5151515151515151No Hit
GTCGCTCGTTGACCAACTCGATATCCGTGGCCCGCAACACCACACCCGCC11.5151515151515151No Hit
CTTTTCATGTCTCTGAAATCCGTGCTTTCCCTATGCTCTTTTCATCAGAT11.5151515151515151No Hit
ATGCTGTACCTGCTGTACTTCCTGCTGCGCGACGGCGCGGCACTGTCCAA11.5151515151515151No Hit
AAACAATGCACCTCTCCTATGCTGACAGAGCCGCATATGCAGGCGACCCC11.5151515151515151No Hit
GTGCCTCCTGGTCCGACCGCATCGTGCGCATGTTCAGCACCCAGTCGTTC11.5151515151515151No Hit
GCTGATGACGGTGCGCGTGCAATCGGGCGAGCAGCTCGAGGCAGCCGCCG11.5151515151515151No Hit
CTACATTACTGTGAACGTGATTACAAAAGAAATCGATCTAGATCTCCCAA11.5151515151515151No Hit
GATTAATGCATTATCTCCGCCTGATATGAGGTCTATGCCAATAATCATCA11.5151515151515151No Hit
GTGGTGGCCGAAGGCGTGGAGAACGAGGCCCAGCGCGACATACTGATCGC11.5151515151515151No Hit
GGTGTAGCACCTTGTCCAGTTGCTCCTGATTGTCCGCCTTGCTGCCGAAG11.5151515151515151No Hit
CATCAGCGGCATGAGCCCCAAGGATCGTGACATCGCCATGGTGTTCCAGT11.5151515151515151No Hit
AAAACGAATATGTCCTTTGACGACATGTGGACGATGATGAATGATTATCG11.5151515151515151No Hit
GGCCCATGCTGATGCTGGCGATCGCGCCAGCAGTGGACAGGCCGATGCCC11.5151515151515151No Hit
GCCATGGCCACCGGCCAGGCGAAGGCCGGCGACCGCAGTTTCAGGCTGGA11.5151515151515151No Hit
TTTTTCACCGTTGAGGCTGAGCAAGGCGCCTTCAGACAGGAACTGCAGCT11.5151515151515151No Hit
GGTTAGCACTTTGGGGAACATGGAAACCGAATGAATAAGAGGAGTGAGAT11.5151515151515151No Hit
GAGTGGACCAACACCAGCCTGCTGCAGGCGGCGTCCCTCACCCTCGATAT11.5151515151515151No Hit
GTGCTGTCACGATACCTGGTGTGAAGGTGACGGCATCTGTTTGTATCGTC11.5151515151515151No Hit
ATCCTGCACATCTTCATCTGTCATACTTGGATAGAGCGGGAGCGAAAGCG11.5151515151515151No Hit
TCGTCAGCCTGCAGGGTGACGTGGCGATTTTCATCCATGACGTGGCCACC11.5151515151515151No Hit
CTGCATTTCAGGACGAACGAATTTATGAAAATAACAAAGAGTCTGTTCCA11.5151515151515151No Hit
ACTCTATCTCATATCAAAAGGGAAAAATCAGGACGAGATCGACGAAGTGA11.5151515151515151No Hit
ATAGGATCTGATTTCAACGTGAGTATTTTATTTTTGTGTTTAATTCTCAT11.5151515151515151No Hit
TTACGGTTGTTCCTTTATTAACTTCGCTCTCGATATCGATTGTCCCCCCA11.5151515151515151No Hit
GCCTGGACAGCGTGAAATAGCGCACTGGCCGAAAGCCTGAACCTTCACGC11.5151515151515151No Hit
GCTCTTTTCTTCCTTTTCCAAAACAGCGGATAAACCCCATGTTCAGATGC11.5151515151515151No Hit
GACTGTTACTCTTTAACCAATGCCCCACGCGAGGGTAAAGCACCCCACCG11.5151515151515151No Hit
GCTGTAACTGGCGACGGATTCCGGAGCGATATTCAGTGCGACGTTGCTGC11.5151515151515151No Hit
ATATATGGTGCAGTAAGTCCGAAAGTCATCACACATGCTAGCAGTCTGCG11.5151515151515151No Hit
GTTCAAACACAAAATGCGGTTGCGGCTATGCTAAATAAATATCCAAAGGG11.5151515151515151No Hit
CTTTAGCACCTGCGATCGGCACGGCACACGGCATTTATAAAGGGGTGCCG11.5151515151515151No Hit
CATCAAGAGAGTTCGAAATTATTTTAAGCATCTGAACGTTCCAAAACGGC11.5151515151515151No Hit
GAAAAGCAATCGTAGAATTTTTTGTTCGTTTTGGCTGGACTGGATTGATC11.5151515151515151No Hit
ATCCAGCGCCTCGCCGAGCACTACATCAACCTGTTGCACGCCGTCTGCGA11.5151515151515151No Hit
CCGCTCAGCCCGGCCAGTACGCGCACGAAGTCACCCAGCAGCGCAGCACC11.5151515151515151No Hit
ATGTTCTTCACGGCCTGCAGCTTGGGCACGTACATGCGCGTCTCGGCGGG11.5151515151515151No Hit
TCAGTGGCGTGTGCATCAAAAGTGGAATCGCCAGGCTCGACACCAGCAAC11.5151515151515151No Hit
TAGCAATAAATTCTAAGGTACGTTGGCACTCCCTTAATCTTTGCCAGTTC11.5151515151515151No Hit
GTCGATATCCGCGGCCCCTGGCCTGGCGTTGAGTGAAGCGATACCCAGGG11.5151515151515151No Hit
GACCTTATATGGGCAAGAAGACCGCACTGAATTTACCTATCAAGTGGATC11.5151515151515151No Hit
ACCTGGGGGTGATCGGCATCATCGGCATCATCCTGCTGATCGGCACCGCC11.5151515151515151No Hit
GGGGTGATATGCCCCCACAGCTTGTGCAAACCAGTCTCACCCTAATCTGT11.5151515151515151No Hit
GTGCACCGTTAACTGGAGCATCTTGACCGTTGCGCTCAAGAACAAGGGTA11.5151515151515151No Hit
TCACAGAGCGCTTCTTCAACAGGTGTAACACATCCTGAAGATCTGTCCGG11.5151515151515151No Hit
CGCAAGACCAGTTCCATCTTCTTTATAACGCATGCATGCATCATAAATGG11.5151515151515151No Hit
CAATATTATTGCTGGGGCTGTTTTATTGAATTGACACTGTCAAAAGGCAA11.5151515151515151No Hit
TCTGTATTTGGAAGGGATCATTCCTCAGCAATAAACCACTCATCTGTGCA11.5151515151515151No Hit
GTCCCACGGCTCGAGCATGGCGGCGTGGTACTCATAGAACGCGCGGCGGC11.5151515151515151No Hit
GGGGTTTGTTATGTTTATTCGAACGGAGAGCTTTCAACAATTTATTAGAT11.5151515151515151No Hit
GGCCTGGCGTGCCTGCTGTGCCTCTTTTTCGGCCTGGGCGATCAGCCCAT11.5151515151515151No Hit
GTTATACGCACGCAAAAAAGGTGATAAACGGCTATTTATTGCGCAACATG11.5151515151515151No Hit
CACTTGGCTGATTGCAGCTGTCCTTCGTGTCTCTTTAGGGTCAGCGACTG11.5151515151515151No Hit
GCCCTCGCCACAGGTAGCATCGGCTATCCCCTGCCCAGACACAGGCGGCG11.5151515151515151No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)