FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n02_Tr4_C4I3c_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n02_Tr4_C4I3c_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40
Sequences flagged as poor quality0
Sequence length251
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTCTAACCCTTCACCCCTCTCCTTGTAACCCCCCTCGTCCCCCCCCC12.5No Hit
TGCGTTCGCGGTCCCGGTGCAGATCCGCTACTGCCTGACTGTTCTCCACT12.5No Hit
GGCGCGGGGATGCATGACATTTGCGATCCTCCCACTTATCTGTCCCTTAT12.5No Hit
ATCGAAACGTTCTGGCAGCCGCATGAAAAAGCCGGCGTGGAGCGTGCATT12.5No Hit
GTCCTTGATCGACGGCACGATCACTACGTCCTCACCATCCACCCAGTTAG12.5No Hit
ACACCAGCCAGCGCGGCAACGCCGACCAGCCGAAGCCGAATGCGGCCATG12.5No Hit
CCCCAACCCCTCTCCCCCGGGAGAGGGGTTGGGGTGAGGGTACGGGGCGA12.5No Hit
GCAAGACACACCGCCCCGCTAAACTAGGTGTCTGTTGAGCTTGCCGTTTT12.5No Hit
AGCGATGTACGCCGGTTCCCTAGGCGGGGGTTTTTTCCGGGTACTGGCCC12.5No Hit
AACCTGGTCAATCAAGCTCCGTCCTCCACCGACGGTTTCATCCAGAGTGA12.5No Hit
GTATTCACCGATGACCATGGCAGCGGAGGTCGCCAGTGTCACGCCGACGA12.5No Hit
GCTCACCCATTCGACAAGGCGGCCTCTGCGGCTGCCGATCGCGGCGCGCG12.5No Hit
ACCACGAACAGCAGTGAATAACCGAAGCGCGACCCTGCTTCAATGGCTGT12.5No Hit
ACAACGTCCCGGACCCCACCCCGTCCCTGCCGCGCACTCGGTTCACCACG12.5No Hit
GGCCAGACCGCCGGTTCCCAGCTGGATGCCGCCGTACCAGCGAATGACCA12.5No Hit
CAACAAGATCCTCGCTTCTGTCCCGGCTCTGCGCGCAGAAAACCCTCATG12.5No Hit
TGCCATACCGGGAAAATCATGCAGATCAACGTCTTGCGAAGCCTGATGCA12.5No Hit
CAATACCCTGGTCAGCGTGCACTCGGACGCATTGGCCCATGCGCATAGCT12.5No Hit
TCGGGAAGCTGACGGTGATGGCGAAGTTGGAGGTCCACTGCGCGGCACCG12.5No Hit
AGACGCCGGGCTTCTCGATCCGCGTCCAGGCTTCGTGAATGAACCCGGGT12.5No Hit
TCTTTATATAGCTTGACCAAACGCTGGCGAAGCGCGAATCTCGCAGGCTC12.5No Hit
GATGCTCCGCGTCCGCTCTATAATGTGGCGCAGATGCGTGGCCAAGCTCC12.5No Hit
ATGATCTTCTTGTCTTCCTTGAACGTCTGGACCGGCACGTCTTTTTCGAC12.5No Hit
CTCCAAAGGAAGCAGCTCTATGCCATTCATAGTCAGGGAAGGTGATACGA12.5No Hit
AGTGACCGCGTTCACCGCGCCGAGCACGCTCGGTCATTCGTGTGTGTAAA12.5No Hit
TTCCAGCAATGGGCAGGCGAGGGCAGTCGCTTCAGAATCGACTTGCCGTT12.5No Hit
GCCGCTGTAGCTGCCGTCGGTGCCGGCACCGCCCTCGCGCCAGGCGCGCT12.5No Hit
GGCGAATGCCCTGCGTTGCGGCACAGGCCGGGATAAACGCCGTTATATTC12.5No Hit
GCGCCCGATGTACGTGGACCTGTACAAGCGCTCGTTCTCGCGCGAGGCCG12.5No Hit
ACTTACAGGTGCCGCCCTTGTGGTGCGAACCTGTTCCACCAGTTGGATGC12.5No Hit
GAGAACAACAAGTCGGAGCTGGAAATCGTGCTGAAGTATTTCGAAGGCCG12.5No Hit
ACTAACTCGTCCAGTCGGCTCGGGCTGGCCTTATGTACTTTTCTGCCCCA12.5No Hit
TCGCCGATGTGCCACCCTATCACTTCATGGCGGCCGGCAACGACGTCACG12.5No Hit
ACCGAACACCCCGAAGTCGTCGGAAAACTCTCCACCCTCGACCGCTATCT12.5No Hit
TCACCTACCGGGGAACTTCGCATCACCCACCATCGTCCCTTCCTGACCGC12.5No Hit
GTACATGCTAGAAGATTTGGTGCCTGTGTCTAAAGAGCTACTTTCAGGTT12.5No Hit
TCACGAGGATTTGCTGGCGCCGTTGGATGTTGCTGCCATCGGTTGTGTGG12.5No Hit
CACACATTTATTCGCGATCGATGGCCTACAGGCACTGCTCTAGCAGGTAG12.5No Hit
GACTTTGAGTCGTCCTTTACCTCAATCTACGCTCAACTCCCCTCCGTCTT12.5No Hit
ATGTCCATCTCTCCTGATTCCGCATCCGCTCCCGCCCCGGGCCGGCGCCG12.5No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)