FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n02_Tr4_C4I2c_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n02_Tr4_C4I2c_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95
Sequences flagged as poor quality0
Sequence length251
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGGAAATAGCCGATCGCCACTTCCGGCATGCCCAGTCGGGTGCGTTCG11.0526315789473684No Hit
GTCAGCATGGCATCACGGTAAATGGCGTAGTGCGATTTGGCATCCACGCA11.0526315789473684No Hit
TCTTTCGAATGTCCAGTAGATCAGACATCTCGGCCCTCTGCATGCTCCTT11.0526315789473684No Hit
AACAGCAGAAAGTCAGAGGGCCTAAAACCGCAGGCTTCTGCCTGGGTGAT11.0526315789473684No Hit
GGTTACTACGGCGAAGGCCAGCTGGACACGCAGGGCGACCCCTCTACCGC11.0526315789473684No Hit
GCCACACTCATAGCTGACGATCCGAACCTTGATGCCGACCTTTGCCCAGT11.0526315789473684No Hit
CATGCGCGAACATGGCACTGGATTCTCTGACTTCCAGCTCTTAGCGCTCG11.0526315789473684No Hit
CTGGGAAACTCAGCACTTCTACTTTTTGAGGCAACTCGTCACGCTAACTT11.0526315789473684No Hit
GACCAGTTCCTTGCGATTGCCTCGGGCGCGCTTGACTGCCTGATTGATCC11.0526315789473684No Hit
CATAACCATCGCGCACATGTTACGCTGCACGCCGGGCCGTGACCAGCCAA11.0526315789473684No Hit
GGTCAGCGTGGGCTTGGATTCGGGCTTGAGCAGCGTGTCCACGGTGCCGG11.0526315789473684No Hit
TCTATCAGGACACCCACGCCATGGCACCTTTGCTCAAACGTATTTCCTGG11.0526315789473684No Hit
GGTCAGGGAATGCTCCAGCAGGAACGCCTGCCCTCCGCCGGCATAAGCAC11.0526315789473684No Hit
GCGCATCGTCACCCTCGCCGAAGCGCACCAGAATCGCGCCACGTCCGGCA11.0526315789473684No Hit
GGAAATGCGCGCACAGCAGTTCGGCCACCGCCTCCACCCCCGCCAGCACC11.0526315789473684No Hit
GCATACGGCAGGCCGGTCAGTGGCAACGTGGTGACGAACGATTCGTTGAT11.0526315789473684No Hit
GTACAGAGGACGCCGGTTGACAAGGCGGGACCGGCGTTGCTTTTTGCAAG11.0526315789473684No Hit
CTACTTCGGTACCTAACGCTATCTTTTTAACTACCCTCCTCACTCCCCCA11.0526315789473684No Hit
TCTCGGTGTATCGCGAGCACTCGAATTTTTCCAACGGCATGCACCCTGAT11.0526315789473684No Hit
GGTGGGGGTGACCATGCGGCGCGGCGTCTACACTCCGGCACTGGCGCTCC11.0526315789473684No Hit
CGGCATTTCCCATGGATGCCACGGTGTAATCGACCATGGTGCCGTCGTAC11.0526315789473684No Hit
ACACAGAACATCGATCCGCGCAGTATCGGGCTGACCGCGGATCATCTCGC11.0526315789473684No Hit
CGATTTGCTCCTTGATCTTGCCGGTGCTCTGCCACTCGGCCTTCTCCGAC11.0526315789473684No Hit
CCCCTATGTTAGTTGGCAACGCATGATACGCATTGGTTCCCGCTCTACCG11.0526315789473684No Hit
GGGCGGAGTAGATCCGGGGTCCGCATGTTAGAGGGAGGTCGTGCCTCGCC11.0526315789473684No Hit
GGAATCTTCCATGGACTTAACGTACGTGCCGCTGCCGCCGTTCCACAACC11.0526315789473684No Hit
AAGCAAAAGAGAGATGAATGGAGCGCTCAACGCAACGACCACCGTGCGGC11.0526315789473684No Hit
CAACGACGCCCTCGCCTGGCCCATTGCCGAAAGCACCAAGCGCCACACCA11.0526315789473684No Hit
GTATATGGTTCAAAGTAGGTTTTCTTTCTGCGTTCATGATTCTAGACAGT11.0526315789473684No Hit
GTGCGGCGCCCGGTTGGCCGAGATGACCACGTATCTGCCCCAGACCGTTT11.0526315789473684No Hit
ATCTTGACGTTTGACGGCCGTATCAGGCCAGCCCCAAGTGCCCGCTGAAC11.0526315789473684No Hit
CTGTGAAACGGGCAAGTTGACACGCATTGAACATCCACTCCCTGGTAAAT11.0526315789473684No Hit
CTGAAGTTCTTCGACACCACCGAGCGCACGCCATCACGCAGGATCGAGAC11.0526315789473684No Hit
GATACGCACCGGCTGGGACCGGGAGAACAAAAACGGGCTCACCGTCGCAA11.0526315789473684No Hit
GAAAGCCTCATTCACATGCACGTCCATTAGACGCTAACCTGTCCCGCAGG11.0526315789473684No Hit
GATCGGAACCGACCACCTCTCCCCTTCAGGCTGAGCAACGATGACCGACG11.0526315789473684No Hit
GTCTTGATGACTCATGCTGTTCCCTCATCATGAAGAGTGGATGTATTGAC11.0526315789473684No Hit
GGATGGGGTACGCGCCGAGTTGCCGGACGGTTATCTGCTGGAGGTTGGCG11.0526315789473684No Hit
ACCCAGTATGGATATGTCCAGAGGCTTCGCCGGATTGACCCCGATGATAT11.0526315789473684No Hit
GAAGGGGGGAGGGTATTGTCAGACATCTTCAACCTCTGCGTCCCAGATAA11.0526315789473684No Hit
CCGCAGCGCGGAAATCGCTCTCTCATGCGCTCGTCTTGCTTTAGCATCGC11.0526315789473684No Hit
CACCTGGAACCTGCCGTTCCAGGTGTGGCCGTTCGTGGCGGCGGCCGCCA11.0526315789473684No Hit
ATTCGACACTTTCGACGTCGGAAACACCGTTTGAATGTGGCCGGTGTGCA11.0526315789473684No Hit
GGCCGCGACCCTTGAAGGCGCCAGCTCTCAGGCAGGCCCGGCTCAGCGGC11.0526315789473684No Hit
TGGCCCAACCACCCCTTGCTACCACCCCCGCCCCTTCAGGGGAACGATCA11.0526315789473684No Hit
TCCCACCAGTGGTTGTAGGCGCTGGAATTACGGAAGCTGGTCAGCACCAC11.0526315789473684No Hit
CCTCGGCACGCAACTCGTTGCCGCGCCGCTCAAGCTCGATCCGGGCGGCG11.0526315789473684No Hit
CAGGAAGTGAGCAAGATGATCGCCGACACCGACCCGGCCATCTGGCGCGC11.0526315789473684No Hit
GCTGTGAATCGTCCACTCATACATCGACACTATGTGCTTTTGCGATGGAT11.0526315789473684No Hit
CGATACAGGTTCACCATGATGCTGGCGAACTGGCCGGCCTGCTTGCCCTT11.0526315789473684No Hit
GGCTCATACAGCCTGTGCACAGAGGCAAGGGGCTCAAATCAGGTCTTTGG11.0526315789473684No Hit
GTATTCGGTTAGATCCAGCGCCTGTTCGACGGACAATTGCTGTTTGCCGA11.0526315789473684No Hit
CCAGGACCGTCACCTGCGTTCCGGGCACCGGCGTCAGCTTTTTTGCCGTG11.0526315789473684No Hit
GTACGGTGCCGCAGATTATTGCCCGGATACTGGAAGAGCATGGCCTGCTG11.0526315789473684No Hit
CTGAGGAAAAGAAATAAACCGAGATTAGCTTAGTAGTGGCGAGAGAAAGG11.0526315789473684No Hit
CCGTGCAGCAGGACGTTTGGACCATCGATTTCCTCGAATGGACGCCCGCC11.0526315789473684No Hit
ATTATAGATAAATGCAGTATTGATTTTCCTGCAATCAACTTGAAGATAGA11.0526315789473684No Hit
GGTGGGCACTGCGGGTGACCCCGCCCATCGGGCTTCCTGCGGAACGGTGT11.0526315789473684No Hit
GCTGCATAAAGGCGCAGCAAGTTACCTGCTTGTGGTTTCAAGCCCCAGCC11.0526315789473684No Hit
GAACAAGAAGGCTTATCGCAAGATCGCTCAGAAGCTGCACCCGGACAGGA11.0526315789473684No Hit
CCCTCACGGCACTGGCTCATTATCGGTCAGTCAGTAGTATTTAGCCCTGG11.0526315789473684No Hit
CCCCACAGCAATACTCTGTCCAGCGCCTTGGTGTAAGTGATCAGGCTTTG11.0526315789473684No Hit
GTTCAGAACCGGAGCCAATGCTGCTGCGGATCCCAACTCCACCAGAATCC11.0526315789473684No Hit
GGCACGACCGAGTTGGTTCTCAATGTCCGGAAAGGTCGAACGGATGAGCC11.0526315789473684No Hit
CTGCCCAGTGGAATCACCTTGCGATCGATCGCCACGCTGTAGCCCGCCGT11.0526315789473684No Hit
GGGCATGATCATCTCGTCAGTGGATCAGAATAACCCCAGCGGATGGGCGT11.0526315789473684No Hit
TTACCAAGCTCTCTATTACTATTTATTTCAATTCTCTTCCGCCCTGCCGC11.0526315789473684No Hit
ATCCAACATTGGGCAGGAGCTCGCTCACATCGTGCGAGCTGACCCGAAAA11.0526315789473684No Hit
ACCCTGGGCGGCAACCGCGGCGGCAAGTTCGCGACCTACCGCAACCTGTT11.0526315789473684No Hit
GGGCAGGCTTCGGTCAAACCTGAGCAGTTTCGCCAGGTCTACATCCCGCT11.0526315789473684No Hit
AAACCGGGATCGCGTGTGGTGCGCAGGGTAAACAGGTTGGGGGTCACCGG11.0526315789473684No Hit
CTTGAATTGGGTTCCTGCGTCCTTGATAACCAGACAGGTGAGTTACTGGC11.0526315789473684No Hit
CAATCGATGCGAAGCAGCTGCCGTGCATCACCCACAGGCCATGCAAAGCA11.0526315789473684No Hit
CCACCTGACCAACACATGACGCGGCTACGTCGACTTCACCCTGCCCCCCC11.0526315789473684No Hit
GGCGGGACGCGTCCCTGAGCAATCGGCCGATGATGAGTGCGCGCCATCTG11.0526315789473684No Hit
GCATTGAACCTGTCGCTGGAAGTCAACGGCGCAGCGCGCAAGCAATCGTT11.0526315789473684No Hit
TGTACGCACGCTGCTGGGTACGGGCGTTGACGACATTCTTCTGGTTCCGC11.0526315789473684No Hit
ATACGGGCCTGTCGGCACGCCGTAGAACCTCAGCGTCTGACCGAAGACTT11.0526315789473684No Hit
GGAGAAGGGGCTCGACATCAAGGTGGACTGGAACAGCATCCTTGCGACCA11.0526315789473684No Hit
CGCCCCAGCCGTTCGACTTCCGGGCGACCCGGATCAACCCGTTGTGGACG11.0526315789473684No Hit
AGATATGCGGATCCTGTGGGAGGCGCCACCACGTCTTCGACGTAGCGCTG11.0526315789473684No Hit
ATGGAGTGATGCATGGGTGGTTTTGTGGCGGGGGACGAGGGTGTCGCATA11.0526315789473684No Hit
GTCTGGCCCGGTTCCAGGCTCCGCCATGCACTCCGCACACCATCCTCACC11.0526315789473684No Hit
CGTGGATGCCGAACTCGTCCAGGACGAAAACCTCGTGGCAAAGCCTGAAG11.0526315789473684No Hit
GGTTGCGCTGGCGATGAAGTTGCCGCCGCTATCCACCGCGGCGCCCACCA11.0526315789473684No Hit
TCGCCCAACTGGGCACCGAACTCTTCGATCGTCGCGCACTGCAGATCCGC11.0526315789473684No Hit
CTTCGATGGCCGAAGGCCGCCGCGACGGCAAGCAGTGGATCGCCTCGCTG11.0526315789473684No Hit
CGTCGATACTGCTCAGTTCATCAGAAAATGGCAGGGGCGGATGCACGGGG11.0526315789473684No Hit
GACCAAAGCGGATGGCCTGCGATTCGCTCTGCCCTCTGGCGAGCGCCGCT11.0526315789473684No Hit
GGCTATTTGGCGGTAAAAGCGCTGTAGCTGTTCACCAGGTTGCGGTCTTT11.0526315789473684No Hit
TTTAGAGCCCCCGCCCCATCTCCACGCACCTAATAAGCATCATCTGCATG11.0526315789473684No Hit
TTCGAACTGTTCGCGCGAGATGGTGGCGGTGGTGTCCCGGCACCCATGTT11.0526315789473684No Hit
CGCTCGCCGGGCTGCAGGGTCACGAAGGGCACGCTGCGACCCTGATTACC11.0526315789473684No Hit
AGAGAGGGAGAGCTGTGGGCGGGAACGGACGACGACAAACCCCACGGAAA11.0526315789473684No Hit
TTCCGGAGAAGGCGATGAACCAGGCGCTTGCGAAATTGGCTCAGGTGGCG11.0526315789473684No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)