FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n02_Tr4_C4I1b_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n02_Tr4_C4I1b_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35
Sequences flagged as poor quality0
Sequence length251
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTACTCATTTTTGTGGGGTCGGTGTGGCACTGGCAGAAAGGGTGCCGCT12.857142857142857No Hit
GTCGCCAGATGCGCAGAAACGGTGGACTGGGAAAGCCCGGCACGTTGATC12.857142857142857No Hit
TCGCTATATGCACTTGAGCTCGAACACCTCTATCCCCATGCTTCAAGTGA12.857142857142857No Hit
AAATCTCGTTCTCGTGGGCTCCGCCTGCGGAAGTAACATCATGCGGCTGG12.857142857142857No Hit
GCCCCGCACCTGTCATGCGTGGGTGACAGCAAGGCTGACCTGCGCGGTCT12.857142857142857No Hit
GGTGAAGAAACGGCCCCTTCGGTGAGGATGAGCTGACGTCAGTGACCGCC12.857142857142857No Hit
AGATTCAGCTGATGGGCCTGTATGGCGCCTTCCTGACGGCGGACCTGTTC12.857142857142857No Hit
ACGATCTTCTGGTAATCGGCGGCGGCATCAACGGGGTCGGCATTGCCCGT12.857142857142857No Hit
TTGCAGATGCAGGCAATGGCTGCGGTGCGGTAGTCGCCATATGCCCCGAA12.857142857142857No Hit
GGACTGGTACCTGGCCGCCGGCGGTTTCGTGCGCCGCAACGATTGTTACC12.857142857142857No Hit
GCTGTAGCGAGTATTAATGAGAAGACAACTATTAATGCGCGGCCACTGAA12.857142857142857No Hit
GTCGCAGTTTCTCGTTGTGCCGCCCTCTCAAGCCCCCTAGGCGTCCACCC12.857142857142857No Hit
GCCTTGCGCAGTGGCAACGGCAGCGGGTTGCTGTCGCGGGTCGTCAGTTT12.857142857142857No Hit
GTCGAGGAACAACCCAGCACTATGCGGATGCGTTTTCAGTCGCTGAGCGT12.857142857142857No Hit
TGCAGGAAGTCACCAAGTTCCGCGCCGCAACGCATCCGCTGGAGTACCAG12.857142857142857No Hit
GCCAACACCGGCATCAGGCGTGGCTGGACTTCCTCAAGAAAATCCTGTCT12.857142857142857No Hit
TCATTCTTCGACGGTTGGATAAGGCAAATCATGCAAACCAACCCGTAGAA12.857142857142857No Hit
ATTCCAATGCGGTCTTCAGACCCAGGAGATACACCCATGAGCAGCACTGG12.857142857142857No Hit
CTTATTGGCTCACCCAGCAAAGCGGAATACCGGTAATCATGGATCGATTC12.857142857142857No Hit
GTAGGCGATCTTGGCAGGGACCTGCAGTTCCACTTCGGTACCGGTTGCGA12.857142857142857No Hit
GATTGCATCTGATACACCGCGTATTTTCGTTCTGCTGCCGGTTCCCGGCA12.857142857142857No Hit
CACCCATTTCGCGAAGAGCAGCCGCAGCGCGGCGGATATGGAGGCCGCGG12.857142857142857No Hit
TCAGAACACTGATCGACAGAAAAGCGGCCATGACATGACGGCGACAGAGA12.857142857142857No Hit
GCGAAGGCATCGACAAGGAGCTGGCGAAGAAGATCGTCGCTCACATCAAG12.857142857142857No Hit
CCCTTGTCATAGCTGCCTGGTTTTGCAATGAACGCTTTCGCGGCATTCGA12.857142857142857No Hit
GGATTAACCCGGCCCAGGAACCCTTGGCCATTCGCCGGACGAGTTCCTCA12.857142857142857No Hit
ACCTGCAATATGCCTATTACGCGGCGGGCGGGGCGCTGACACGTATCAAC12.857142857142857No Hit
CATATGATTGACCCGGCGTTATCAAGAAGGACGCATCGACGTCCCGCCCA12.857142857142857No Hit
CTTCAACATGTACCACGAGATTCCATCGGTCGCGAAGAAAGCAGCCTGGG12.857142857142857No Hit
TCACTACACGCATCTTTTCTCCCGCTGGAATCGTCTTTTGTCAGCGTCAG12.857142857142857No Hit
TCGAATTCCTTGCCGTCGATCACCGGCGGAGCCCCGAACCGCTCGAAATG12.857142857142857No Hit
ATAGGAGTGTATCGAGGCACAGTAAGGGCAGGGCCCCCGTGCCGGCAAAC12.857142857142857No Hit
GTTGACTTGCTTTCTGAGTGCCGCCTTTGCTTGGCTGTTTCTCAGCCTGG12.857142857142857No Hit
ATCCTGTCCTTGCGACCCAGCGCATTGGCCGACAGCGGTGCCAGATCCTG12.857142857142857No Hit
GGAATTGGTGGAGCTGTTCTTCGAGGAAATCAGGCACGCTCTGGAAGACA12.857142857142857No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)