FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n02_Tr4_C3I4c_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n02_Tr4_C3I4c_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41
Sequences flagged as poor quality0
Sequence length251
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTGATGGATCTGGCACTGCCAGATTTCATCGTGCAGCTCTTCCGGCA12.4390243902439024No Hit
TCCTTCAGGAACAGTTCGAAGGTGTACGGGTTGAGCTTCATGCCCTCCTC12.4390243902439024No Hit
TATGAAGGAAGAAACGCCAACTTCACAGGCGAGGGCTTTGCTCAGCAAAA12.4390243902439024No Hit
AATAAAGACAATAAAAAAACCTGTAAAACCTGCATAAATCGTACGAAATG12.4390243902439024No Hit
GTGATGACCGCTGCCATGAGCATGATCGTGGCCGCTGTGGTCATGGTGAT12.4390243902439024No Hit
GGAAGAGGCCACGCTTGACAATATCGCAGCGGAAATCTCCCCCTTCGAGC12.4390243902439024No Hit
GTCGGACATGGTTTTTCCAGAGCCTTTGGCTGATGAACCGGCAACGCATT12.4390243902439024No Hit
TTCAAAGGTTGTCCGGCTCATTTAAATGAATCAGGAGAACTTTCCTGTTT12.4390243902439024No Hit
GTATACCTTTGTGAAAGATGCCAAGCCGGGCGATATGACCGGCGACAAGA12.4390243902439024No Hit
GATCAGCAGCGGCTTGAGGTTGGCGCGGGCAGCGTAGACGGCAGCGCTGT12.4390243902439024No Hit
GCTGGCTGAACAAGGCATCGCCGGTCAGGCATTCGGAGTGCAGACGGCCG12.4390243902439024No Hit
GTTCATAGGACTCACCGCTTACTTTAGCAACAATATAAGCAAGAACAGAA12.4390243902439024No Hit
GGCCTTGGCCTCGGCCTTCTCCTGCTGCTGACGGTTCAGCTCCTGATCGC12.4390243902439024No Hit
GATAAGCCATCAGGTCCTCTTACCTCTGCGATTAGCAAAAATCTCAGAGG12.4390243902439024No Hit
GTACTGGCATGGGCCAGATCCACAGCCTGATCGAGCATTGCCGGGCCACG12.4390243902439024No Hit
GCACCACGCGATGCCGTCGACCGCGAGCGGCTTCTGCATCTACAACGACG12.4390243902439024No Hit
CTCCTACTCCCGTGACGAAGCGCGTCGCGTGCAGACGATTCGCATGGGCA12.4390243902439024No Hit
CACTGGGCTTGAGACAGATCGGCTTGTAGGCGTCCAGGGCAACAGGTTCA12.4390243902439024No Hit
GCCGTATTTAGAAGTAGAGCCGCGAAAATACCGTTTTCGTATTTTAAATG12.4390243902439024No Hit
CCATGATTGAGTGCCAGGCCCCTTCCTGGTCAAGGCGCAACCTTCCTTGA12.4390243902439024No Hit
GCTGTAGGTTGCTCGTCCAAAGGCGGCGACAACGCCGGTGAAGGCGCTGT12.4390243902439024No Hit
TCATGACACTGTCACAAAGCTGGCTGACTTCTTCCATGATGTGGCTTGAA12.4390243902439024No Hit
GAATGGCCGGCTGGTTGGCGATTAAAAGCCGCGATTGTACTGCATCACGA12.4390243902439024No Hit
AAATTGCACTAGGAAATATCCCGCCTCAATTTGCACTTCCTGCACTATTT12.4390243902439024No Hit
GACTCACCACGAGCGATCCGTCGGCATCGGTGATGAGCATCCCGAGGACG12.4390243902439024No Hit
GCGCCACCTTGCTCAAGCCGATTGCCAAGGGCGAACACCACGAAAAAACC12.4390243902439024No Hit
GCCCTGTTCCACGTGGAACAGAGCGACTGGCGTTTCCGTCTGCCAGCCTT12.4390243902439024No Hit
CTACGTCTGACGCCATTAATTTGATGGCACTCTTGTAGACAGAATCACAG12.4390243902439024No Hit
GTAAATGGTTTCCCTGTAAAAGTAAAACCAGGGGCTCAAGAAAAACATAT12.4390243902439024No Hit
GGATGGAGCAGTCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGG12.4390243902439024No Hit
CGGCCAACGCCCTGTCCTGCCCAAAGAGCCATTTGATCAGGGTTTCCCGC12.4390243902439024No Hit
GATCACGGCCATGGCGCTGCGCATCAATGGCATGTCGCACTGGATAATGT12.4390243902439024No Hit
GCATCTCCACTGACCCGGTGCACGGCTTCATCTTCGTCAACGACATGCGT12.4390243902439024No Hit
GGTGAAAAACCAACCCCATCAGAACGGCCTCCAGTTGAATGGTTTATTGC12.4390243902439024No Hit
GGTTGAGTTTGGCCGGTGAAGGCAAAGTGAGGCGTGGAGCGGTCATGCGT12.4390243902439024No Hit
GCGATATACGTTAACGCGGCAACGGCGATGCCGACCAGCGGCGCGACCCA12.4390243902439024No Hit
CATCAGTACCGCCATGTCGGTTGACGCGACGCCGCCGATGCCCAGATGCA12.4390243902439024No Hit
GTAAGCGCTGTGAGGCGTTGAGCTAACCAATACTAATTGCCCGTGAGGCT12.4390243902439024No Hit
CGAGACTAGAATCGGGATCTTTGCAGCACCGTACCGCAGAAGCCGCCGTT12.4390243902439024No Hit
AGACCTGACCGTGATCCAGCGTACCGAGCATCGCCCGCACATCGGCGGCC12.4390243902439024No Hit
GTAGAAAAGGAGCGGGCCTTAATCATTGGCGCCGGATCAGCTGGAACCAT12.4390243902439024No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)