FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n02_Tr4_C3I1b_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n02_Tr4_C3I1b_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41
Sequences flagged as poor quality0
Sequence length251
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTTCCACCTCATGGACAGCGCGATTGATCATGTCAATGACATCTAGTC12.4390243902439024No Hit
GCACGGGACGCGGCGCGCTTGGCTTGCGGTTAAAATCGGGCTCATTATAA12.4390243902439024No Hit
GTGCAGAAGCGCTCGGCTTCCTTCACGCGCCACTTGGCGTAGGCATCGAC12.4390243902439024No Hit
GGCTTGAAGCCCGTGCACCGTCGCGTGCTGGTCGCGATGAGCGAGCTGGG12.4390243902439024No Hit
TTCCGGTGCGCAGTCACGAGAGGCCCGAACGCGCGCTTCGCGGCTCGCCG12.4390243902439024No Hit
GAACTGCACCCTGGCGCCGATGTGGGTGCGCAAGATGCCCAAACGCAAGG12.4390243902439024No Hit
GTATTTGAGCAATACTGAGTGGGTCGCTCACGTGAAGAGTGGCAGCTACC12.4390243902439024No Hit
AATATAGAGCGGCCAATAGGCTTGAACATCGGTGCCCGTTATAACTTCTA12.4390243902439024No Hit
CATTGAGGCTGGTTGATGTAATCCGACATCTGGAGTTTCAGGAAACGCGC12.4390243902439024No Hit
GAAAGATGCAGTCTGGCCGTTCCGGACTTTTCCGACGTCAGCCTCGTCAA12.4390243902439024No Hit
CATTCCACGCACCCCGGGCCTGGGCCTGACACTGGATGAAGAGCGTCTGG12.4390243902439024No Hit
GACGAACGCGGGGAAGGCGCCGGGGAAGGCTTCAACTTCAACTATCCGCT12.4390243902439024No Hit
GGAATACCGCGATGCGGCGGGCGTCGACGAAGGCATGAACGGTCTGTCGA12.4390243902439024No Hit
GCACTGGCGTTTCGTCGGCGTTTCGCCGGCGGTTAAGCAGTATCTGCTGG12.4390243902439024No Hit
GGTATTCCAGGCGCTCCTGCAACTCGACCTTGATCCCCTCGATGGCGTCG12.4390243902439024No Hit
ACCCAGACAGAGGAAGTCCAAGGCGAATTGCTCAAACGAAGCCCGCGACA12.4390243902439024No Hit
ATGTATGAGAGAATGAATAAAAGGCAGCATGAAAAATAGAGAAAGCAAGC12.4390243902439024No Hit
TGTAATAGCTCTGGAAGGGCAATGACAACTGCTAGTGACGTATCTTTTAA12.4390243902439024No Hit
AGAAGCCGGGCTTCAACCTTGGCTACATCGCCTACAACGTCCGTCACAAG12.4390243902439024No Hit
CGCCTCGACCATGTAATCCAGCAACGCCTCGGCATCCTGACGCCAGCGCG12.4390243902439024No Hit
TCCCAGTTCCAGCTAAAAGCAAGCAAACAACTAGTAATAAAACCTTGCCT12.4390243902439024No Hit
AACACTCGGTTAGTTGTTGGCCAAAGCGCGTGTGCCGACCCGTTTCTGCG12.4390243902439024No Hit
GCCGTGTTCTAGGGTTTTTGGCGTATTGTGGGCACCATCGGTGATTTTCA12.4390243902439024No Hit
TTTTCGTTCTGGGTCGCGACGCCGGTGGCGCAGTTGTCCAGGTGGCAGAT12.4390243902439024No Hit
GACAAGAGCGGAGACGAAAATTTCGGATTTAACGCGCATGCTTACGCATC12.4390243902439024No Hit
GTCTAAGCGCGAGTGGCTCCACCAGGCAGGGTAATCTGCGAGAGTCGGTT12.4390243902439024No Hit
CCGATGCCTTTCGAGCAAGGCATCGGCGTCCCAGACCGTCATTTGAGCGA12.4390243902439024No Hit
GGACGGCGACGGGGATTGGTGAGGATGTTTCCGGGATATGGCTGGCGGGA12.4390243902439024No Hit
GTACTTTTCGGTGTGCAAGAACGTCGCCGAGCCGCCGAGCATGAGCCGCG12.4390243902439024No Hit
CTCAAAGGCTGGATCGTAAACGCCAGGACCGGCATAACCGAGGTCACCGC12.4390243902439024No Hit
TCAGTGTGCCGAAGGTGCGGGCGGGCATGCGGGTGAGTGCGACCATTTCC12.4390243902439024No Hit
GTCGCGTTGTCTCTTTCGTTTTTTTTTTCGTGCCGCCGCGCCCTTCCTCT12.4390243902439024No Hit
CTCCCACTCAACAACTTCATTCTTTATATGAATGACGAAATAACTAGCTA12.4390243902439024No Hit
CAACAAAATCGTGGTCATGAGTTTCATCTACACCAGTTGCACCACGGTCT12.4390243902439024No Hit
GTACACCGCAGTCTGGTCGTTCATCGAACGGTTCCAGGTTGCACCGGTAT12.4390243902439024No Hit
GGCCCACACAGTGAAAAAGGTTTCAGGAATAGCCGCAGCCTGAATCCAGT12.4390243902439024No Hit
GTGTTGAAGGTGCCGGAAGCGTCGGTGACCACGAACACTTCGAAGCCTTC12.4390243902439024No Hit
CCAGTCTTCAAAGCGCGTTGGACCCAGGCGCGGGTTGCTGCTGCCGGGGG12.4390243902439024No Hit
CTGGGCGAATGCCGCCAGTTCACGGTACTGAGCCAGAGCGGTACGGATAC12.4390243902439024No Hit
GTTAACTCTTCACCTCAACATGATCCAGCGCTTGATTCACCGCCAATTCA12.4390243902439024No Hit
GTATCATTGAAGGCAAAACGGCGATTCTCATCGATGATATCATTGATACA12.4390243902439024No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)