FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n02_Tr4_C2I3c_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n02_Tr4_C2I3c_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33
Sequences flagged as poor quality0
Sequence length251
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAATATCGGTTCCGGCTCGAAAGCGCCTCTCCACGCATAACGCCCAGA13.0303030303030303No Hit
CGTCAATAACGATGAATTCGGCATCCTCGGCACCGCGTTCAACCGTTTCG13.0303030303030303No Hit
GAAGGAAAAGAGTCGTGCTGATCGGCATGTAACGCTAAATACTGATCCTG13.0303030303030303No Hit
GCGCGGCCTTGGTCAAAATCGTGAACAGGCGTTCATCACCGCCGAGCAGT13.0303030303030303No Hit
CCTTATGGAATCAACGAGCAGTCACTAGGCGAACGATTTAGTGCCCCTTC13.0303030303030303No Hit
ATTACGATCTGCCGCAATATCGCCCGTTCTGGGGTGAAGTCGAAAAGCTC13.0303030303030303No Hit
CTTTTTATATCATGCACTATGGCTAAGCCGGACCCCTGCTCATTTCCCTA13.0303030303030303No Hit
CCTTCGTGTTTTTCCGCTGGCTTTCCTGCAGCTCCACCGGCATTTCATCC13.0303030303030303No Hit
GCTTCCACCACCATCCGGTGTCGATCGGCTGTGCATGGATGGACCCCATC13.0303030303030303No Hit
CTTGTCGATCAGTGCCTGCAGCATTTCACGCTCGGGCGAGAACCAGAAGC13.0303030303030303No Hit
GTATTATGAGAATTTTCACTCCGGTGAGCGGCCGTCGTAAAACCTGTCTA13.0303030303030303No Hit
CTGTCATGTAATTGCCATCCAGCTTGATCGCCAGGATAACGTTAAATTTC13.0303030303030303No Hit
GCTCCAAATCGCGCTGCGCCGCGCTGTCGAACAAGGCCAACGGGCAATGG13.0303030303030303No Hit
GCCGGACTCTAATCCAATCTGGAGGAAATTCGCAGTGGCAGGTCATTGGC13.0303030303030303No Hit
GTGTAAAATCTCTTCCTGTGTATGTTCGTAACGGAACGACACATCTTTAA13.0303030303030303No Hit
GTAAATAGACGCAACAAGACCTGAAATGCAGTGAAAGCCATCCAACTTCG13.0303030303030303No Hit
CGGCGCGACCAGCATGAAGAACAACACGAGCCACAGGGCAAGCTCCGCCG13.0303030303030303No Hit
GTCATGATCATGCCGAGGCTCGCATCGTCGGTCGGCACCAGATCGCCGCC13.0303030303030303No Hit
ACCGATGCCTTATTTTTTCTAGGTTTAGCCGCCGTGATTTCGACTATTTC13.0303030303030303No Hit
CGCTCCTCCTGCGATCATTTCCTTCAGAATGGCGGCAAGCCTCTTGCCAC13.0303030303030303No Hit
CATTAACATTGTATTGAGCAGAAAACGAATCACTGCATCAATCCATCTGA13.0303030303030303No Hit
GCCTATGCTTCATCCAATGTGGCAGTTCTCAACCACACCATGCGCGTCTC13.0303030303030303No Hit
GCTGCCATCAGCGCTGCGCTTGCCGAAGCACAGAACGCTTTCGCTCCCCG13.0303030303030303No Hit
CCAAGGAACCGGCGCGGATGGTCGACTTCCTCTCGGTCGGCCCCAACGAT13.0303030303030303No Hit
GGAGACGACATCTGCGCTCTCTTGGCGCACCTTTCCCGCTTCGACGTACA13.0303030303030303No Hit
TTTCAAAACAAGGCCCATGGCGCGCCCGTTGGATGAACCGCCAGGAGTTG13.0303030303030303No Hit
AATTATCTGAGCTGGCAAAGGAAATAAGTGATACACAGTCACTGGAAGAA13.0303030303030303No Hit
TCTTTTAATTCATAAGCAGAGTTTGCATCCGCCATTAACGGGCGGTGAGG13.0303030303030303No Hit
GAATGAGAGGGGTTATCCATACATGGAAGTCCTAGCGCTCGAAGCGGTTC13.0303030303030303No Hit
GAGCTGGATCGCATCCCGCACGAGCCGCCCGCCTGGATGCGGCGCCCCGT13.0303030303030303No Hit
GCCCTGGCCTACCCGATGGCATGGCTGGTACTGCTGGCGGTAGCCCTGGG13.0303030303030303No Hit
GCATATAGGCGGGCGTGCTCCCCCGTTTGCGCGCTTTATCCTCGACGATG13.0303030303030303No Hit
GCATAAATCAGAGCCGGTTGCCTCAAGATCGAATGTGCATCGTCCGAGAA13.0303030303030303No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)