FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n02_Tr4_C1I2c_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n02_Tr4_C1I2c_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123
Sequences flagged as poor quality0
Sequence length251
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCAAGGTAAAGAAGAGGGGAGCTTTATCATTGAGGAAGATGGCAAAAA10.8130081300813009No Hit
GCCCGGCGGCCATCAGGGAGCTCTAGTTCTGTTCCAAGCGTCGCACATCG10.8130081300813009No Hit
CATTGAATATGGAGCGGGAAACGAGACTCGAACTCGCTACCCCGACCTTG10.8130081300813009No Hit
CGTGAAGAACATGATCACTGGTGCTGCCCAGATGGACGGCGCGATCCTGG10.8130081300813009No Hit
CCTGACCTCGAGCGAGACCCGCATGATGGCCAAGCTTGCCCCCGACTACG10.8130081300813009No Hit
GAGGTAGATCTTGGCTCCGTCGAAGCATTCGGACAGGCGTTCGGCGAAAT10.8130081300813009No Hit
CCCTTGAGGTCGAATTTAAATTCGACGATGCCACTTTCAAGAGCCTTAAC10.8130081300813009No Hit
GCTGCGCAACCTGCCCAAAGTGTTGCGCAAGAACTTTGTGCCGGTGCCGG10.8130081300813009No Hit
GTCATCGGGGGCAAGCCCCCTCCCACATTTGATCTGCTGTGTTCTTTAGA10.8130081300813009No Hit
GCTACAAACATAAACAATACGTATAAAATACGCAATCGCTTTTTGGAATG10.8130081300813009No Hit
CTGCTATCAGGTGCCTGCGCTGGTGCTCAACGGCTTGACGGTCGTGGTAT10.8130081300813009No Hit
CTTCCGCACCGGCGACATCGTGGCCATCGACGATGAGGGGTTCACGACCG10.8130081300813009No Hit
CAATATCTCTGGATGCATCGCCGATTCAAAACACACCCTCTTGGCGAAGC10.8130081300813009No Hit
GTTACGGTACCTGGCGCTATGGAATGAAAGCCACCGGCGCGATGGGTGAC10.8130081300813009No Hit
TTTATTACGCGTACTTGACGGTGCTGTAACAGTACTAGACGCACAATCTG10.8130081300813009No Hit
GTTATATTAAGAAAGATAGGAGGGTTCCATATGTTTAAAACAATACCTTT10.8130081300813009No Hit
AGCATGATGAGCGCACTGATAGCGGGTACCCTACTCTGTAGCTTCCCTGC10.8130081300813009No Hit
ATGCAGAAAATACGGCACCGTACTCAGCTGAAGCCCTGATCGTCTGTGTT10.8130081300813009No Hit
CTTTCCCGCACGTTTACTTTTCACATAAATATTACAGTCATCTGCGTAAC10.8130081300813009No Hit
GAATGGCGGCTCGTCGAAACTGCGCAGTTTGCGCGAGCGCAGCGAGTTGA10.8130081300813009No Hit
TTCATGGTGGGGCCGGTGGCGTGTGAAGTGACCGGCATCAGCTTCGCGCC10.8130081300813009No Hit
CCTCCGAAGCCGGCAACGAGAGTGCCTTGCCCCAGAGCCTGCACGGCGGC10.8130081300813009No Hit
CCGATAAAGAAGTCATTGGCTCGTACGCTGAAGCGCCGGCGCAAGGTACT10.8130081300813009No Hit
CCAAGATGCGCGCCAGTCAGGGCAGCGTGCGTTCCAGCCTCGCCGGGTGC10.8130081300813009No Hit
GATCGATATTGCTGCCGCAGCGTCCGGCAATCGGTTTGATGGCGTAACCG10.8130081300813009No Hit
ATAGTCGATTGCACTAGCAGATGGTTACGATCATAACTTATATTCATTAG10.8130081300813009No Hit
GGATTCATGGATGTGTTTGCCGGTGGACAAGTCCATCACCGTGTCCGAGT10.8130081300813009No Hit
CCCTTGACCTCGTTCATCAACTTCTCGATACGGTCGAGCATGGCGTTGAC10.8130081300813009No Hit
GAGCAGGAGCGATTTATGGGGATGGTCTGGGGTTACCTCAAACGCTGGGG10.8130081300813009No Hit
CTACACAGCCCTCTCTTCCCTGCCCCCGTCTCCCCACTTGCCCTCGTCGC10.8130081300813009No Hit
TCGTTGAACTTGCGCACCGAAGAGGCGCAGGTTGGGGTCTCGACGCTTGG10.8130081300813009No Hit
ATATAGGACTTGGCGACACGCAGGTCGATGTTCTGGGTAGTGACTTTCAA10.8130081300813009No Hit
GTCTTCACTGCAGCGCCAGGTCTTGATGCGCCCGGTGGTTCTGAGCAAGG10.8130081300813009No Hit
ATATAAGCCGCTCAAAATAAGGTGGGTCGGCGTAGCCGATCATCGGCGGG10.8130081300813009No Hit
ACCGTCGTACGTCTCGCCCAACCCGACGATGCCGAGGGAATCAGCCGGGT10.8130081300813009No Hit
GTTCCATTTTTGCTTTCTTGTCTCAATTTTCATCATCTTTCTTTCAGTCG10.8130081300813009No Hit
TTTCAGCGCTATTCAGGGCAACGGCTTCAAGAGCCTGAAAGAAGGCCAGA10.8130081300813009No Hit
CTCCAATTGGATGGCAGTGGCCCCGACCTTTAGGGTCGGCGGCGGGTTGT10.8130081300813009No Hit
CATTCTTACAGAAACGTACCTCCGACTTTAAAGGACGTTAATCGTGGAAT10.8130081300813009No Hit
GACTTACTGCACAACTTCGGCGCCGACTACAAAGCGATTTTTCTGTCTCT10.8130081300813009No Hit
GCGTAAGGAAGCGTTGAGCCATGAGAGATAATCGTTGTCGCCGTGGACCA10.8130081300813009No Hit
CATCTGCACCCAGGCCTGGAATACCGAACAAGATGCGGGGCGGCCGCTGG10.8130081300813009No Hit
AATAAGCAGTCAGGGCCGTCATACACGAAGACGAGTCTATAATTGGGATG10.8130081300813009No Hit
GTCATATATTTAGTAATGGTTCTTATGAAGCAGATTATAAATTTTCCCAA10.8130081300813009No Hit
GTTATAAATATCTTTACATTATTGTAAGTTTTTCTATTCATTAAGTCAGA10.8130081300813009No Hit
GGCTATAAAGAGCCGCCGAACACCCGCGAATCCGCGCTTTTCGCGCTGAA10.8130081300813009No Hit
GTAACAGCGTGGCCAAAATGCTCAGCAGCACAAAAAAGCGCCACAGGCCG10.8130081300813009No Hit
ACCGTAACCCGGCTATATACTCCATCAACGTGGCGCAGACGGCCTCACTT10.8130081300813009No Hit
ATTGAATGAACTGTTGGGCCTCGTCTTTTATCAGCAGCCCGCGTGTCGTC10.8130081300813009No Hit
ATATTGAAGAGCTGAAAACCCTGGTGAACGTGGACTGCGGCACCCGTACT10.8130081300813009No Hit
TCACCATATAGATGGCCAGTGCATCTTCGTCCAGATGGGCTTTTCTCTCG10.8130081300813009No Hit
AGTCACCCGCCTGACCCCACCCGACGTGACCTGTCACCGCAACTACTTCT10.8130081300813009No Hit
TCTTTTTATCGTTTAAAAACAATAAACAATATGTTATTATTGCAACAAAT10.8130081300813009No Hit
GCGTGTTAAATAAAGTGGATTTTAAACAATGCATAATAATATCTCCTTGA10.8130081300813009No Hit
TCCGGAGCTGGCCCTGCTGCCTGTACCCACTGCAGTGCTGTTGCGTCGGG10.8130081300813009No Hit
GTCGGAGGCAGTAAAAGTAAGTTTTCAATCGGGTTGAACCGGCGTCTGAC10.8130081300813009No Hit
CTACAACATGGGTCCCGAGCGCGTTCAGCGCATGAGCACCGAAGCCAGGC10.8130081300813009No Hit
GGCTGGCGCCGGCGCCGACTCACCGTAGGTGGTCATGCCGATCACGCGGC10.8130081300813009No Hit
TCTCCAACGCGCACAAACATCTGCCCTCGGAACTGGTCCCTCTGCACGTG10.8130081300813009No Hit
GCCTTGAATGTAACGACCCACCTCGACCAACGCTGGACACGACAGTTACT10.8130081300813009No Hit
AGATTAAACTCCGAAACCCCCACTGTGAAAGCAGTTGGGGTTTTGTTTTG10.8130081300813009No Hit
GACATCGAATTCGTGCGCGCCGAAGGCTTGGCCTACGGTGACGAAATGCG10.8130081300813009No Hit
CTGCTGTTCTGCACCTTGTTCATGGCAACACCTCCAGCAGGTAGTCGTCG10.8130081300813009No Hit
CACCGCGCGTTCACAACAGCGGTCACTGGACTCAAAACGGGGCGACCATC10.8130081300813009No Hit
GCGGATGAAGCTCGGAGACCGTCAGGCGGCCCGGCCAGCGCTCGCTGACC10.8130081300813009No Hit
GTTGTAGTGGTCTCAAATAAATTATTTAACATGGCAACCTTCTTTATTTC10.8130081300813009No Hit
TCCATATACTGCCAGGAAATAATGGGGATCAGTATGGAATAACGGGCGGC10.8130081300813009No Hit
CTACCAGGCCGTGGGGTTGCACGCCGCCACGTACCTGCTGCCGGTGCCGC10.8130081300813009No Hit
CGCTTTCGGACACGGTCTTATTGAGACCGCGCGAACGCTCCCCGATGCCG10.8130081300813009No Hit
CTTTAGTGGCCTGCGCCACTTCCCCATAGCACGTCGGAAGCCGCCCCCGG10.8130081300813009No Hit
GCAGAGCAAGTTTTGCTGGCGCAATTGGGCGACACCTACAGTCATGTCTG10.8130081300813009No Hit
ATATCGGCGAGTACCCGGGCTACGATCCGCTGCAGTTTATGCTCGATGAA10.8130081300813009No Hit
GGTTAGCGTTAGTCATTATCGTGCTGGTGTATATCCCGGTGGCGATCGAC10.8130081300813009No Hit
CTTTAACACTTCACGTTGGTGTTACTTTAATGGAAACGATTATTGGTTTT10.8130081300813009No Hit
CATTTCCGGTGTCCAGTGTCTATCTCTCAACACCTTTCTTGACATGGTGA10.8130081300813009No Hit
CATTTACCTCATTCGTTGCAACCGCTTTACTCACTGCATCTAATAATAAA10.8130081300813009No Hit
GTGCAGTTCCTTACGGCGGACAGCCCGTGGGGCAGCGCTTCCTGGCCCTC10.8130081300813009No Hit
GCGCGTAACCGGTGAGGAACAGGCTCGCCATCTCTGCGCCGTGGCGAACA10.8130081300813009No Hit
CCATTGATCGTTTCAGACATGCTGGAGATCGACAGCATCCCGGATGTGAC10.8130081300813009No Hit
GTCCAGGACGCGATGAACTCTGCGGGGGTCTCGGAACTCATACCCTGACG10.8130081300813009No Hit
GCACGTGGTGCCGCGATTCGCAGCCGCGTTCGTCGTCCGCCTGTTCCCCG10.8130081300813009No Hit
CCTGGGTTTCGGTGACCCCGGCCGCCTTGGTACCTGACGCTGCGGCCAAC10.8130081300813009No Hit
ACCTCCATCAAGGACATCTTCGCCGGGGAGCCGCAGCTCGTCGCCATCCT10.8130081300813009No Hit
CCGTGCTATCAGATGCTTGCGTCGATTACATCGGTACGCCAAGACGGTTG10.8130081300813009No Hit
TTAGTCTGATCGCCTCGGGCGGGAGCAATGCCCTTACATACACCCGCCCC10.8130081300813009No Hit
GAGAAATACCGGCGTACCGGCGGCTTTGGGGCAAGGGGCCAGAGCCACGC10.8130081300813009No Hit
ATATTCACCATGATGCCGGTAGAGAGGGTCCCTGGCAGCAAGCGTAAACG10.8130081300813009No Hit
CTCATATGGCGTGGAGGTTACGATACCCGGCACAACACCGAAACTGCCCC10.8130081300813009No Hit
ATCCATCACCTTGCCAAGGAACAGCATTGCGATCTGATCGTGGTCGGCAG10.8130081300813009No Hit
GTGCAGACTCTTCCAATCCGCCCCAGAACTTGCCTCCCCCTATCTAGTCC10.8130081300813009No Hit
CACTAGAAGTGAGGCATTTTGCCAAGAATTGGCTGAATGCTTCCTAAATA10.8130081300813009No Hit
ATTCCCAAGCGCGGCCTGATGCTGGCGCGCATGGTCGGCCACATCACTTA10.8130081300813009No Hit
GGGTAATGCCCCATGCGCCCTTCTCGCTCTGCCGTTTGAACAGCGCCCGC10.8130081300813009No Hit
ACATTGAATACACTGAAGAACAAGTGATGATCCGCGATATGGCGCGCGAC10.8130081300813009No Hit
GAATAATCAGGCAGACCGGCAGCGCGCGGATTAGATCGATCAGAATCGCT10.8130081300813009No Hit
CTATTTGCCACCCTGACCGCCGCCTGCGCTATGACCTGGGCGGCGGCGCT10.8130081300813009No Hit
ACAGGACGGCGATGCCGAGCTGAAAAAACTGCTGGCGGTGGCTGACCCTG10.8130081300813009No Hit
TAAGTCGCGAGGAGGTTGGCGCAGAATTCCAGCAGGCCCGGCATGACCTG10.8130081300813009No Hit
TCATGAGTCTGTACCGCCGGGCGTACACGCTCGCGTACGGTATAGAAGCA10.8130081300813009No Hit
GTGCAGGAGTGCGCAGCCCCTCGGCCTTCAACAGCTGTGCGCAGGTGCGC10.8130081300813009No Hit
ACCTACCACACCCCGGCTCTGCTGCGCCTGTGCCCAGTGCAGGCCTATGG10.8130081300813009No Hit
CACTTCACCAGTGCCGTGGTCATTTCCTACTTCTTCTTTGTGCTGGTACT10.8130081300813009No Hit
GAGTTCAAGGTTCCCGTGGTGGTCGATGTGATCCTGGAAAAGATCACCAA10.8130081300813009No Hit
GCGTCACGCAGCGAATACCTGGTGTGCATCGACGGCGACGCGTTGCTCGA10.8130081300813009No Hit
GGCTTCACGCCGACGGGTGCCAGCGCGCTCTATGCAGTCAGCACCTGGCA10.8130081300813009No Hit
AGTCTATTCTGCAACTGCCCACCCCGTTGGCCCTGTCGGATTGGCGAGAG10.8130081300813009No Hit
GGCTGAGCCAGGTCTCCTGATAAATTTCCTCAGCCAGTTCGGCCTTGTTG10.8130081300813009No Hit
GGCCAGGCCGTGTTCCAGGGCTTTTTCGGCGGTCAGAATCGAGCCCCCGA10.8130081300813009No Hit
GTCCTCCAGTTTGCCAATGCTATTCCACTCTGCCCCCCCGCCGTATATCT10.8130081300813009No Hit
ACAAAGGAACTCATCATCCCGGTGATCACCGCCTCGGCACGAGAAGTCGG10.8130081300813009No Hit
GTACGCGGTTTTTCAAAGGCTCGAACAGAACGCCGCGGCTACGCAGGTCT10.8130081300813009No Hit
GCGTACCGCGGATCAGGCGGCGTTACCCGAGCGTCTGGTGGCACGGGGAC10.8130081300813009No Hit
GTGTGATTATTTCGCTATGGGTCGACGGCAATTTCAAAGCGTGGAAGCGC10.8130081300813009No Hit
GTTGAGCTGTCTCGTGGGGGTCGCTGTCCCGTCGGCGGTGGTTGGTCTGG10.8130081300813009No Hit
CTGGTGGACCTGATCGAACTGGCTCAGCGCAACGGTCTGGTGTAGGGGAG10.8130081300813009No Hit
GGTCACCGATTCCGATACCCCAACTGTTGTTGATAATGCGCGCGCCGCTG10.8130081300813009No Hit
GTCACTACTGATAATGGGATATTTTATTCTAAACATGTTATCATCGTATC10.8130081300813009No Hit
TTCATCTCCTGATCTCAGGCTGCCGGCGTAGTGCGGAGCGACTTGAGCGC10.8130081300813009No Hit
CCCATTACAATTAAAGTTTAACAGAGAATATATATCCTAAACGAGCAAGA10.8130081300813009No Hit
CGAAAATCGTATCGCCCCTTATCCGTGGCCTTTCATGGCCCTCCCTCTAC10.8130081300813009No Hit
CTTCTTGTGCTGACACGCATAGGCCGAGCCGGCCGCGCTGAGGGCGCCGG10.8130081300813009No Hit
CAATGTGTGGCGTTGATTAACCGATGATTAACCGGGCGCTATTCTAAAGT10.8130081300813009No Hit
TCTTTTCATGGTGCCAAAGCTCCAGCGTGTCTGCCGCCTGCTCTGCTTCG10.8130081300813009No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)