FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n02_Tr4_C15I4c_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n02_Tr4_C15I4c_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31
Sequences flagged as poor quality0
Sequence length251
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACAAGACCGATCACAGCTTCAAGATTTTCCGTGCCGAGATGAGCGTCG13.225806451612903No Hit
TAGAACAGTCCGTCATGCATCCTCTCGTTCCCCCTCCTCGATCACCCCCT13.225806451612903No Hit
TCACTGCAGCAGCGACTGGTGCGAAGAGCCATGTCTGCTTCACAGACAAA13.225806451612903No Hit
CGATGGAATCGACATCGGCAAAGCGTTTGAAGAGAACCGATTTGCCCTCC13.225806451612903No Hit
CCGCAAGGTGATCTTCCGGCACATCCTGCCCAACGCGATGAACGCCACGC13.225806451612903No Hit
ATACAGGAAGCTCTACTTCAATGATTCCACATAAAAAAGAAAGATCTACA13.225806451612903No Hit
TCCGATAACGGCGAAAACCGCCGACAAAGCCGTGAAGGCGAAATCATAAC13.225806451612903No Hit
ATGCGGCACGACTCGAATCGGCCGGCTGCCGCCCTGACGTCGCAGGCGGG13.225806451612903No Hit
ATATTGTTCCCAGATCGACTTACTCCACAACTCAATTCGATTTGAAACGC13.225806451612903No Hit
GCGCCAGCATGAACGATACCGATCGGCGCTACGAACAGCGCGGCGATCAT13.225806451612903No Hit
CGCAGACCGATGCGCTGGCCTCTGGCTACGCGATGCCCGGCGGCGAGTTC13.225806451612903No Hit
CCCGTGGATCGTTTTCGCATTGCTTGCTCCTTAATGGACAGCAATGCTTT13.225806451612903No Hit
AACCCTCCGTCGCAGGTGGACCGATGTTGCAGACCATACTCTCTCGTACG13.225806451612903No Hit
GTCGAGAAAATGGAATTAGGTGACGTTGCACCAGTTTACATTGTTGATGA13.225806451612903No Hit
GGCGCTGACCACCATTTCACGCAACAGACCTTCGTTGTTGACCCACAATT13.225806451612903No Hit
CCGCCTGCGCGCGCAGCTCATGCAGCTGCACCCCGCCGGAGAGGAGCGGT13.225806451612903No Hit
GGGTACAGCTGCGGCACGTCCATCACCCACGAGCCCACGTGCGCATCGAT13.225806451612903No Hit
ACCCAGGCGGCAGCAATTCAGGAACAACGACGTTTCTCGCGCCAGAACGA13.225806451612903No Hit
ACAAACCATACCATCAAACCAGTGTGCAGACACCACGGTCTCTACTGAAT13.225806451612903No Hit
GGCAGTCAACGAGAATTATTCGGACATTGCGTCTGGCAATGGGCATGGCA13.225806451612903No Hit
GGACAGGCTGGACAGGGCGGTGGCGATCAGGGTCAGCAGACCCAGGCAGA13.225806451612903No Hit
GAAAGCATGGCGCCGCCCATATTGCCGGCGCCAACGAGGACGAGAGGCCC13.225806451612903No Hit
CGCTTCGCGAGCGGGGGATATGGGTGACGGCTATTCGTCCACCAACCGTA13.225806451612903No Hit
GTAGGCCTGTGCTCTGGTCCTTACGCAATACTGACACACCCCTGCGAGTC13.225806451612903No Hit
CGCTGAACTGCTCTATTTCACGCTCCAGCACTTCCCGCGAACGACGCACC13.225806451612903No Hit
TCCTAGGGGGGTATGACCACACAAGCGGCCGTGACCCGATGCTGCCTAAT13.225806451612903No Hit
AGGTATGGTAATTGGTAAAGGCGGATCTGAAGTTGAAGCACTTCGTAAAG13.225806451612903No Hit
AGTCACAGGGGTAGTCTGTGCTTCCATCACAAGAGCATCTTGCTCTTTTT13.225806451612903No Hit
AATACAGCCTTCTCGTAGCCTGTTAATTTGCCGTTTTCATCTAAGTAAAC13.225806451612903No Hit
ATCGAAGAACGGTCGAAGCTCGCGCGACATGGGCACAGCCAGAATGAAAC13.225806451612903No Hit
AGGCGACATAGGCAATCGGTGCTGCGGCCCAGATCAGGTGTTGTCCGTAG13.225806451612903No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)