FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n02_Tr4_C15I3c_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n02_Tr4_C15I3c_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99
Sequences flagged as poor quality0
Sequence length251
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTGTGCGACTACTTCGAAGCCCTGGCGGATGACTGGGAAATCCGCCT11.0101010101010102No Hit
GTTTGAAGGAGTATCGCCATGTTCAGCCGAATCCTGCTGGCCGCCACGTT11.0101010101010102No Hit
AATCAACTGGTTGGCGCCATCGCCCAGGAACAGCTGGAGATGGTCGACGA11.0101010101010102No Hit
CTGATTCAGATCGACACCCTGCGCGTGATCATGTAGCTCGACCAGTGCCC11.0101010101010102No Hit
AAGCTGTCCTCGGTTTCGAATATGGCTATTCGCTTGCCCGCCCGAATGCG11.0101010101010102No Hit
TCGCAAAGGCAATGGATCGCCAGCAAGTGGACTTCCTGAATACGTGCTGT11.0101010101010102No Hit
TCCGGATACAAAGAAACAAAGCACTATTTAGAATGAAACTGCTCCCATTC11.0101010101010102No Hit
TAATAATAAAATACGCTTTCTGACATCTAAACGTTTAGCCGCAGGGTGAA11.0101010101010102No Hit
GCTTCTACACCGTCTTGATGATTCCAGCCGCCCGCTTCGCCTCGGAAAAT11.0101010101010102No Hit
GGAGTTGCTTTGTTAGATCACCAACTGTCACAGGATCGAACACTTCTATA11.0101010101010102No Hit
ATATTGGACGGAAGTGGTTCGCTAAAAGAATGAGCGACCTGAGAAGCGAG11.0101010101010102No Hit
CCACTACTAAGTCGAGCCCCTTGTGGGGTACTGGCGGGGAGGGCAGGCTC11.0101010101010102No Hit
TGCGAGAAGCGCCATTTTAATAGCTGGACAGTCAGGCAGGGTCCTATTTG11.0101010101010102No Hit
CAGCGGATGCGGCACTTACAAGTTGACCGTCATGTTCAACGGCATAAAAA11.0101010101010102No Hit
CTCTATGAAAGCTTAGGCTTTAACGAGGCGACTATCCGCCGTAACTACTA11.0101010101010102No Hit
GCAGCGGCCTCTGACGCGAGTACGTTCTGCTCCACATGCCTGTCTCTTAT11.0101010101010102No Hit
GATAGGAGCGCAGCAGCGTGTGTTTCAACAGGTCGTATGGCGTATGCAGT11.0101010101010102No Hit
GTGGCAAGCTCTCCATCGAATCCTGCCAGCGTTCGATCAAGGCGGCTTTT11.0101010101010102No Hit
ATATTTGAATCCACGCTTTTTGTTGTTTTAAGGCTTGCTGTCCTTCTTTA11.0101010101010102No Hit
GGCCGCCCTATGCGTGTCTCGCTCGCGCCGAAACCATGACGGGTCGCGGC11.0101010101010102No Hit
CGTTTATTCGATTCTGGGTCATAGAATTTTTTCTCGTAGTCTTTGCCAAC11.0101010101010102No Hit
GAGTATAAAAATAAGAATCCCTCAAGCCTTAGAATGTTTCTTCATATAAA11.0101010101010102No Hit
GTATAAGTAGGCAGCACCTAATTGTTCGATAAAATCACCCTTATCCCCAA11.0101010101010102No Hit
GACTCAGCCGTGCGCCCTGCAGTTGCATCACCTTGTAGGCCCGTCTCTTA11.0101010101010102No Hit
CAAGATGTCATAGGCGCGTTCCCCAAGCGGTACGTGAACAAGACGTTCTT11.0101010101010102No Hit
GATCAAGGCCCAGCCGTACAGACCAAATCGCGCATCGGGCAATTGCAGGT11.0101010101010102No Hit
CATCGAAGGCGATGAGAAGGTTGAGGTCGAAAGAACGGAGATTGAAATGA11.0101010101010102No Hit
TCAGCGATCCGGCCGCTGATCCCGCGCTCGTCCATGTCGGCCTTGAAAGC11.0101010101010102No Hit
GCCCGGCTTCTCGCCACGCGGATCGACGCCGCAGGAGCTCGCGAAGCTGA11.0101010101010102No Hit
GGCTACAGCTATCCCACGCTGCAACCGATATTCGACGCCGATCGACTCTG11.0101010101010102No Hit
TTTCAAGCGAAGTCTTTTTATCAGCACCGCTGTCGATTCGGTCGGTGACA11.0101010101010102No Hit
ATCGAGCGTGGGATGGGAAAGCTGAGACACGACCGTGGGGTCCTTGACCA11.0101010101010102No Hit
GCTGTCGGCGGCGGCCTCTTTGGCGGCAATGCGCCCGGACCGGCCAATGG11.0101010101010102No Hit
GCATTGACGCGGCTGACTCGGCGCTTTTCTATGCTGCTATTTCCAACCCT11.0101010101010102No Hit
GCGATATTCGGTGTGATAAAACCAGGCCAGCGCCGGATAGAGCGTGGTGC11.0101010101010102No Hit
ATTCGATCGTGCGCGGCACGACCTCGGTCAAGATCGTGCAGATGATCCGC11.0101010101010102No Hit
GCACAGCAAGAAGCGTTATGGCTTCTTGAGCATGCAGCGCATCGGGCTGT11.0101010101010102No Hit
TCATGCGGTGCGTGGCCAGTACGTGGGCGGGACCAGCGATGGCAAGCCGG11.0101010101010102No Hit
CTTCAGCGCCCTGTACGGGTACGACGTCCGCGGCGAAGTCTTCGGTTCCA11.0101010101010102No Hit
GCTGTAGGCATTGTTGCCGACGAAATCGATACGGCCGATCTTCGTACGCT11.0101010101010102No Hit
GCTATTCAAACCAAAACGACTTATCACATCAAAGATACAGACGAGCAGTT11.0101010101010102No Hit
AGTGTAGCTGGTTCTTTCAATCTATTTCTTAATGTACCTCCCACCATATG11.0101010101010102No Hit
CCTCTTCGTAAAACTGGCATTCATATTGCAAAACCCTATCAACAATGAAA11.0101010101010102No Hit
TTTCAGGTCAGCGTCCGAGACTTGTACGAAGTCGCCGTTTTTGAACGCGG11.0101010101010102No Hit
CTTCACCACCCAGTGCCCGGTTCCAGCCATCACCAGCAAACACTACACAG11.0101010101010102No Hit
GCCGATACCTATGTTGAGCTGAACGTGGAACACAAACAGACCCTGGATAA11.0101010101010102No Hit
CGATTAGCGACTGAAGGAAAGGCACTGGCAGATGAAGCCATTCGATTAGC11.0101010101010102No Hit
CATCGGAAAAGCCTGCACTGATACCTGCGCCGGATTCGACCAGGCACTGG11.0101010101010102No Hit
AGTCAATGTTATCTCCCAGTACGTTGAATACACGTCCAAGTGTTACGTTA11.0101010101010102No Hit
CACTGTAAGCCTTCTGTGTATTATATTTTCTCGTTTCCCTGGTCAAATTT11.0101010101010102No Hit
GAGAAAGACCAGCATCCGTTCAAAAGCAAGATGCCGGATTAACGACTGAT11.0101010101010102No Hit
GCTCAGTAATCGCGAGCAACGCAGCATGGCGCGGGTCGAGAGAGCTGCCG11.0101010101010102No Hit
CTGCGCAAACTGCACAAGGCGGCGGGCGGCCTCGAACTGCCCTGAGGCCC11.0101010101010102No Hit
GTCCTACAAGGGCATGATGCAGCCGATCCAGATCAGCGGCGTCGATCCGG11.0101010101010102No Hit
GGCAGAAGGCAGACTGATCGGCGGGAACCTAAATGCCATGTATTGGATAT11.0101010101010102No Hit
GCCTCAAAGCGTGGCACGAACGTACCTTAATGTCGCATCCGTATAACTTC11.0101010101010102No Hit
GCGTATGCGCTGGCGTCGTTGCGCATGAGCCGCCGCCGATCTTTAACTAG11.0101010101010102No Hit
GCGCGGATCCGCCTTACAGGTTCCGCGCGCGTCATCTTCTATACGATGCA11.0101010101010102No Hit
CTTTTAATACATCGGTGTAAGTGACCACGACGCTTTGCCCGACTTTCAAA11.0101010101010102No Hit
TATGAAATACCTTCCAATGTGACAGTGTTTTCGAGGTCTTGTATGACGTG11.0101010101010102No Hit
GCACCAGGGTCGCCAGGCTGAACCACAGCGAGGTGGACAGCCCGAAACCG11.0101010101010102No Hit
ACGTTCGACCTGCCATTTCCAGCCAGTGCCCTCAGCCTTCCAGACAACCT11.0101010101010102No Hit
CCACAGATTTTTCGCGCCCGCGCAGTCCCCTTCTTGCCGTCCGGTAACCC11.0101010101010102No Hit
TGTTATCTCTTTTAGACGGAAAGACGCGCCTCCGGGCACCGACCAATCGC11.0101010101010102No Hit
TGGATATATTAATTGCTACTGAGGATATTTTAACCGAAGAGGCTATGACT11.0101010101010102No Hit
ATAATGCACGGAGCCAAGAACAGAATTCGTATGGGTAATGGTCGCCGAGC11.0101010101010102No Hit
CGCTAGTAGAAGTCACGGCCAACATCATCTCACTTCGCCCACCCATTATT11.0101010101010102No Hit
ACACTGGTCAACTGGGCGGTACCGAGCTTGATGTGCTGGCTCTGTCTCTT11.0101010101010102No Hit
ATCCGCCAGTGCCTGCACTGGCTGAACCGGCGGCTGGGGCAGGAGTTGCC11.0101010101010102No Hit
AAATCACTGTGTAATCGACGCCTCTAAAAACTCGGCTCTTGCAGCCATTG11.0101010101010102No Hit
ATTCGGGACTTCTCCTTGCTCTGTTTGCACCTTGTCACATTCAAGGGAAC11.0101010101010102No Hit
GTGTATGGCTGCGCGGCCGTGATGAAGACGCTCGCCCACTGCCACCGTCG11.0101010101010102No Hit
TCTCAATATTTCTTACTGTCCTGAGCGCGTACTGCCCGGTCAGGTGATGG11.0101010101010102No Hit
CGTGGTCTGTTTCGTGGTTTCTGGCACATCACGCTCGGCGTCAAATGGAT11.0101010101010102No Hit
CACAGGACGATTGAAATTTCTTCGTCATTTAGGGCTTGCCAAGGTCGGCC11.0101010101010102No Hit
CTAGGTGGCGAACCGGACGACTTACGCAATAAGGATTATTTTCTTATTCT11.0101010101010102No Hit
GGTTTTCGACGACGAACTGCGCACCCTGATTGCCGACATGTTCGAGACCA11.0101010101010102No Hit
GTCTCGCGGCCTTCACCTTCTGGGGATGGCAAGCGGTGATCGTCGGCGCG11.0101010101010102No Hit
CATTTCAGGTAAACGGCGAAAAGCAGCACGGCGAGTGTGGCGATCATGAA11.0101010101010102No Hit
CTGTAGCCGCGTGTTCATCTCCATGAAGAAAAACTCGCCGCGCGCTGTCT11.0101010101010102No Hit
GTGCCGCGAACTGCTCGATGACATCGTGCTGGTCTCGGAGGACGAAATCG11.0101010101010102No Hit
GAACTGGTCACCGAACGCTTTCCCGAACTCGACACACTGGTGCACGGGGT11.0101010101010102No Hit
GGATATCCCTGATCTCATAGAAGAATCAAGCACGCTAGTCACCATGAATT11.0101010101010102No Hit
GCGTGAAGGTCGACCTGATGATCGACGGCTTCGGTTCGCCCGACCTGTCG11.0101010101010102No Hit
ACCGAATCGGGCGTGCTCTTCAACTCCACCGCGGTCGGGGTGGTCTGGGC11.0101010101010102No Hit
TACAGGTGGCGCGGCCCGTTCGGGGTAGTACCATTTGCCTTCAAAACTGC11.0101010101010102No Hit
AGAAGAAAGGGCATCACTGTCATTTTCTTCAATCATTTTCCATGCATGAT11.0101010101010102No Hit
ATCATGACGAACCTGCTGGAAGCGATGCGAATTTTTGTCGCGGGGGGCGA11.0101010101010102No Hit
CACCTCGGCGGCGGGCTTGGCGCGTCTCTTCTGGAAGCCATGCAGCAGCG11.0101010101010102No Hit
GCTGGCTTATCATGCCTTCCTGCGCTGGATCGTGCGGGAGCCGCGCAATG11.0101010101010102No Hit
CTTTTCATCAATACGCCCTCCAACCCGACCGGATGGACGGCGACGCGCGA11.0101010101010102No Hit
GCTGGGCGCCGTGGTGCTGCTGGCGCTGCTCTGCATCCAGGCCGTGCATT11.0101010101010102No Hit
GATCAGCTGTTTGACCTGATCCAGTGCGAAGAACAACGGGCTGCTGCCCA11.0101010101010102No Hit
AGATTCAATAGAGCAGCAAATTATGCATAAAAAAATAACCCGCTGAAGCA11.0101010101010102No Hit
GGACAGAGCTTGAGCGTGATGTCACTGGAAACGGCCTCAAAAATCAAGTG11.0101010101010102No Hit
CCTGATGAGTCAGGCCCCACCTTTCACACTCACAGGAATTGCCGGCGGCC11.0101010101010102No Hit
ATGTTTACCTTAAAAGGGTTGTACTAGCGTACTAGCGATGATGACATCAT11.0101010101010102No Hit
CACTAAGAGAGCACGTGAGGAAAGATAAAATATCCGTTACCCTGTCTCTT11.0101010101010102No Hit
CTTCAATGGCATCATCCGCATCCTTTTCATCTGGCGATTGATCGATGAGT11.0101010101010102No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)