FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n02_Tr4_C15I2c_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n02_Tr4_C15I2c_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123
Sequences flagged as poor quality0
Sequence length251
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTATGCCGGGGCGCAGAAGAATCTGGGCCCGGTCGGTATTTCGGTGGT10.8130081300813009No Hit
CGGACTCCGACATCTCTGGATCTGCAGCCTGGTTTTGATGCTTGTGTGTG10.8130081300813009No Hit
GATAAGTCTTTTCCATGACAGCCAATACAGTTTTGCTGATAAATCTCTTC10.8130081300813009No Hit
GGATCCAGAGAGCGCGGAAGGTGCGCTTCTTGATCTTGCGGTCGCGGTAC10.8130081300813009No Hit
CAACCGTATTTCGCACCAGCTGCGATCGCTTTTGTTGCGTCAATCACGCT10.8130081300813009No Hit
CTCCTATCCTTTGTCCAGCTGCTCGTCCGGCGCGGGCACCCGCACCAGTA10.8130081300813009No Hit
GTAGTTCCGCGCTCGCCTCTCTCACAGCAGGTCTCCGTTTCCGCTGCCAC10.8130081300813009No Hit
CCTCAGGCGAGTCCCAGCACGGCAGAGCACAGGGCGTTGACGACCCAGGC10.8130081300813009No Hit
GTTTCACTGAAGCGGCGTTTTAAATTGAATTCCTTGTCATTCCTTGTTTG10.8130081300813009No Hit
AGCCATCCGTTAGAAAGCAAGTCTCCAGCCCGTCTCCCCAGCGGGCTTTT10.8130081300813009No Hit
GCCTCATGCCTAACCCGAAAGTCGGCACCGTAACTCCAGACGTAGCCAAC10.8130081300813009No Hit
GCCCAGCATCTGGCCGAAGTCATTCGCCCGGCACTGGCCCGTGGTGCGGT10.8130081300813009No Hit
ACTACTCAGCATATTCGGCTGCTGTTACCTCCTTAGCAGCCTTCCATTCA10.8130081300813009No Hit
ACGCTGGTCACGTAGACGCGGAGCTGCGACCCGTCGGTGTACTCCTCGCC10.8130081300813009No Hit
GGGCCAGGTAGACTGCGGCCCCGTCCAGGTTGAAGCTGTAGCCGGTGTGG10.8130081300813009No Hit
TTATTGGAACAGCGCCGTTTCCGTTCCCGTGCGGTCGCTTCTGGCAAGAC10.8130081300813009No Hit
AAAAAGGCCCGGAGCGTTCGCTCCGGGCCTCTTCCACGTCTGACCTCCGG10.8130081300813009No Hit
GTCCGCGCTGGCAATCCCGGCATTGGTCAATCAGCCTGCCCCGCAAGCGA10.8130081300813009No Hit
AATGTGGCCCCTCACACTTCACCTTGCGTCAGCGCCGCTCTAAAGAGCGC10.8130081300813009No Hit
ACCATGCTCGGTGCCGATGTGGGCACCGCCTTGATGGCACGGGTGCTCAC10.8130081300813009No Hit
GGGCCAGGCCGTGCGTGTCCTGGCCGAGCCGCCCGGGCCAGTCTGGGACT10.8130081300813009No Hit
GTGCGCGCGCTTCTTGATCGCGGGCGTCGAAGAAGCGGCCCGTGGCCAAT10.8130081300813009No Hit
TAATATGATGAAATACGATCATCGTATTCTTTTAAATAACGATCCACATG10.8130081300813009No Hit
GTCATTGCCCACTTCGATCAGTACGGCCCCGAGGCCTCGGGCATCGCCGT10.8130081300813009No Hit
CTCTGCGGCTGGCGACGCCGACGATGCCATGGTTCCGCGTGGGCGCGCCG10.8130081300813009No Hit
CTCCTTAGATATTCGCCGAGAACGTATCGCAATCCTCGACACGGCCGCTC10.8130081300813009No Hit
AGCCCCACACGTTGTCGGACTGAAAAACACAACATCATCCATCATCAATC10.8130081300813009No Hit
GGAATCCGATCGCGAAGACAACCTAAATGCGGGCCCCCGAGACGCAGCTG10.8130081300813009No Hit
CCCGAGATTGATGAGCCTCCTCGCCGCAGCCGCAATCGGTTTCATTCAGT10.8130081300813009No Hit
GGAAATCTTCGGTGCGGCTAGCTTTGTGGTCCGCTGCGCGAATCATGACG10.8130081300813009No Hit
AGAGAGCGCCGTCCGCTGTGCGATGAACCCCGCTTTATCCCCGCCTGACG10.8130081300813009No Hit
CCTTGCAAAAGGGCGCGTACCTGCAGGATCAGATAAATTTCGACGATCGC10.8130081300813009No Hit
GCGTCATGAGGCGTAAAATAGCTGGATTGTATGGCTGGTAAAGATCAGAC10.8130081300813009No Hit
CTAGCTGCCAGCTTCCCCATCCACCGCCTTCTCTCCCCCCTCGCTCCCGT10.8130081300813009No Hit
CAACGATATTCGCGCACCTGAGCAATACGACGTTCAAACCGCTCCCGTGC10.8130081300813009No Hit
CCGAACTATCTGGACCTCCGATCCCGCGAAACCAGCCCGGGATAGCTGGC10.8130081300813009No Hit
ACCGTACGTCGCAGTCGTACACAACGAGTGTAGGGGCAGCGAATCAAACG10.8130081300813009No Hit
GGATTGATGTCTTTAATCGGTGCCGCTTCTAAACCGTCTAAGAGTTTAAT10.8130081300813009No Hit
GTCTCAGCCTGCACGACCATGCCATCCGGGCAATGCTCGGCGTGCGGTAG10.8130081300813009No Hit
ACGTACCGGTCGACGACTCGACCTATGAAATGAGCATGGACGACTGGATG10.8130081300813009No Hit
CCCACAAGGGATCGCGCTCCATCAGGAGCAACTCGGCCGAATGCGGGAGC10.8130081300813009No Hit
GCATTGGGTGCCGTTACACAGCATCTAACGAGGTTGCAGTGCGCACGCGG10.8130081300813009No Hit
ACATACAATATTACCTTGAAATTCTAATCCTTGTCTTCCAGCACGTCCTG10.8130081300813009No Hit
CGCTGAAGGGGTAACCGAGGCTGATCTCACGGTCATCGAGAGTGCCCTGG10.8130081300813009No Hit
GCAACTACCTCTTCATATTTTCGAAGCACACGCAAAAAGGTCCGCACTTC10.8130081300813009No Hit
GCTTTATCGTGACCACCGACTCCATCGTTGGCGCCCTGAAAGCCACGTCC10.8130081300813009No Hit
CGCTTTTGCGAGGTCTCTCCTCTTTCTAAAGATACTTGCGCTTTATTCGA10.8130081300813009No Hit
TGCGTGACCACAGCAGCTGGCTGATCGCCGTGGTGATCCTCCTGCTGCTG10.8130081300813009No Hit
ATATTTGCATGCATAATGAAATTACCCCTCATATCGGTTATTATTTGGTC10.8130081300813009No Hit
CCGTAATATTGTGGTGATGGGTGTTGCCAATAAACGAAGCATCGCTTGGG10.8130081300813009No Hit
ACCTCGTACTCCGCGGCGTACAGTTCCTCGATGTCTTCGCGCTGGAAGAA10.8130081300813009No Hit
TCTTTCACCTGATCGATGACATCAAGTGGAGAAACTTGCCGAATGCTGTA10.8130081300813009No Hit
GATCACCAGATCGGCCGATCCCCAAATCACGTACAACGCAAAAAACGCAG10.8130081300813009No Hit
GTCCGCAAAAATGCTGGGCCGCGCTGATTGCCTCGCTGGTCTTTTTGATC10.8130081300813009No Hit
GTTGGATGGCAGTCCCATGGCTATCCTTCTGCCTATTCCGTTCTTTAGCT10.8130081300813009No Hit
GCCCGCGCCTGCTCCACCAGCCCCAGTGCTTCCTGATAATCCAGCGCAGT10.8130081300813009No Hit
ATCATATAGTTTCTTGCACTAGCAGATCTTAGCTCTGACTCAACGATTTT10.8130081300813009No Hit
GCCCTCCTTTGTATGAAGGAGATACTCACCCCATGCGGTTCAAAACCCTG10.8130081300813009No Hit
TACGTCAATATATCTTTCTCCACCATTTATATCGAAAGAATTATCCGATT10.8130081300813009No Hit
GTATTGGGCGCGGATTTTCAACAGTGCACCGGGCGTACTGCGCGATCCCA10.8130081300813009No Hit
ATTGAGGACATTAATCCTCCATTGGAACCCAGCCTTTACTCTGTCTCTTA10.8130081300813009No Hit
GATCCGGAGGCCGCTTGCGCACCGGATGCACTCCTCCTGCACTAGAGCCT10.8130081300813009No Hit
AGGTAATGAAGCCCGGGAATTTCAGGCCCATGCCCGCGTCACGGGCTCTG10.8130081300813009No Hit
GGAACAGAGCGCGCAGATCAGCGTCGCAGACACGGGACCGGGCATCGATG10.8130081300813009No Hit
CTCACATGTCGATGCTGCCGGCGCCGTCGCTCTTCGTGGCTTCCGATTAC10.8130081300813009No Hit
TTGCCAAAGACTGGAGAAGAATGGCTACGTTATTTGATGTATGCAGGGAT10.8130081300813009No Hit
CGAGCAACACAATAATCCCCAATGCCGCCTTGAGATCCTTGGCGGTGTTA10.8130081300813009No Hit
ATTCATTTATGCCAGGCAACGATGCCGCCCGAAAAAACCCCGGAACCATC10.8130081300813009No Hit
AGCAAATTGAGGCGGAATGTTTCCTAGTGCAATTTGACTACCAATCAGTA10.8130081300813009No Hit
CCCGTCATTCCCGCGAAGTCAGGGACCCAACCGATTCTAGTGATTCTGGC10.8130081300813009No Hit
GCTGAAAACTTGGTTGTATCAAAAAAATCTAGCGGTATAAAAACAGAGAA10.8130081300813009No Hit
CTTCTCATGTTTGTCTCTCCATGTGTCATGCAGGGCGCGCTCACGCCGAC10.8130081300813009No Hit
CATATAGAAAGTCGGAACGAAAAATATCAAACAAACAGGCCGAACATTCA10.8130081300813009No Hit
CTGTGCGACGTTGCGATATTGCGTTTGGCTGCTGTAATCGCCGTAGGAGT10.8130081300813009No Hit
AGCGGGGTATTCGATTGGGATGACCCATCGCAAAACGCTCGTCCGCGTGA10.8130081300813009No Hit
GTATTACGACTACCAAGAGCCGATTAAAAAAATTGTGCCTCATCGTGTAC10.8130081300813009No Hit
ATATCGGATGAAACGGGCATCCACCGCATCAGTTCAAACCGCTCCCGTAG10.8130081300813009No Hit
GTATTTCACACAGCTGCTCATGCTGATTGTCTCTTGTTTACTAGGGATGA10.8130081300813009No Hit
GGATACACGTTTTACTCTGGACAGGCGTCGGGCCGATTCCCGGCATTGCC10.8130081300813009No Hit
CTCTACGAAATAGAAAATCTTATAAATTTCTTTGATTTTATCTTTTAGAA10.8130081300813009No Hit
CCTGTCTACAGAGCCTCTGAAAACAAGCAACCGGTCTTTGTTCAGCGCAA10.8130081300813009No Hit
GTTCAAAAGAAATGAATGCAATTGTTTATACCGAATCGCCAAGACAAGGA10.8130081300813009No Hit
CCATTGAGTAGCCTGTAATATGGCCTTGCTGAATTTGGTCCCAAATATAT10.8130081300813009No Hit
ACCATGAGCAGCCTGAACATCACCCCGTTAAGCTCGGCCCTCGGCGCACA10.8130081300813009No Hit
GCTCGCACACGCTGACCATGGTCAGGGCGGCAAGCCGGTGGTACTTGGCT10.8130081300813009No Hit
GCTTCCATTCTTTATTGTGGGCTTTGTGAGTGCGTTTATTGTGGCTCTCT10.8130081300813009No Hit
GCGCAAGGCAAGTCTGCGCGCCATCCCAGGCTCACCCAAGGTGAGGAGGG10.8130081300813009No Hit
ATCATCGCCTTTCTGTTTTACAAAAAGCAACAAAATCAAAAACTGGCGCA10.8130081300813009No Hit
GCCTTTATCGCGGCGTTTCGCCAACAGTTTGGTGAGACGCCGGGAGAGTT10.8130081300813009No Hit
AAAAGATACCGTTCCTGCCGGTCGATAAACTGATTTCAGAGGATGAGATT10.8130081300813009No Hit
GCCAAGGCGACCGACGTCATCAAGACGCTCATCACCGACGCCAAGCACTG10.8130081300813009No Hit
GTACCAGGTGCTGTCCATGAAGGAGGAATGGTGACCGATTCTGCTCGTTC10.8130081300813009No Hit
ACGTTGGGGCGCTTGCGTGCAGGCCCGGCCGCAGCTGCTGGCACTGCATC10.8130081300813009No Hit
GGATAATGCAGAATGCACAGAAATTGTTACCCCCTTCTTGGATTTTTAAT10.8130081300813009No Hit
TAGCAAGACTATGCCGCGCTGTCCCTGATGGTGTTGTAAGGAAATCCCTA10.8130081300813009No Hit
GCTTTATCCTCTGCTCATCCGCAGCGATGGTCACATATGATACAGGCGTA10.8130081300813009No Hit
GAGGAGAACCGGGTGTTCGCGAATCACTTCAGCGAGAACGTCCCAAACCT10.8130081300813009No Hit
AAGGTGAAGTAGCCCACAGCCACTGGCGCTTGTCCGGTCTCAGCCTCGAC10.8130081300813009No Hit
CTGGTAGGCAGCGCCATCACGCAGCAATTGCGTAGCCGCCTGATCGACGG10.8130081300813009No Hit
TTTCTATTCTCTCTTAAAATGCGTAATTGTTCACCAAAGTGATTCATGTC10.8130081300813009No Hit
AATGTTATCGGCGTTATCGCAGGCTTCGCCCTCGCCGACCGTACCTGCCG10.8130081300813009No Hit
CTATAATATGAATAGACCTTTTATCACAAAAGGAGTGATGGAGATGAAAA10.8130081300813009No Hit
GTCTCACCGTTTCTTCAAGACAGACGGCTGGTGCCTCTTAAACAATTTTC10.8130081300813009No Hit
CAGCGCGCCTCTGCGCACCGGCAGAATTACACCGTCACCATTCACGATTC10.8130081300813009No Hit
ATAATATCCACAACCTTCATTCTTGGATTGAGGGAGGCATATGGGTCCTG10.8130081300813009No Hit
ACCCAGCGCTTTATAAGAAGAGGTATTGGCTGGCATGGCTGCATGGGATG10.8130081300813009No Hit
GTCGTGGGTCATGACCCTCCCCGGGGGCGAAAGCAGTTCATGCAGCCGTT10.8130081300813009No Hit
GCTGAGAAGCGCGTCCATGGCCTGCCTCTCCAAACGCGGGAAGTGATCCC10.8130081300813009No Hit
GTTTTCTGGGCAGCGTTGACGGGCCTTGCGATGGGTATCAGCGGTCTTGG10.8130081300813009No Hit
CTTCACGTCGATCCGCCGCCCCTGTACTCCGCAGCACACCATCTCCGCGG10.8130081300813009No Hit
CGCGGGACCTGTAACCGTTACCGCGGCTGCGAACGAGCGCGCCTAAGATC10.8130081300813009No Hit
GGCTAATAAACCACCACAAAAAAGGGGAATCGACCAATGCTATGGATTTT10.8130081300813009No Hit
GAGCAACTGCGCTGACCGTAGGTCGACAGCACCTGCCGGATCTGCGGCCA10.8130081300813009No Hit
CCTTTGGTCTGCCGGTAAAATGGATATCAGAAGAAGAAAAAGATCAGGCT10.8130081300813009No Hit
TAGATAGGGTATCCACCATCTGGCCACTAAATAAATATAAATTTTGCTCG10.8130081300813009No Hit
GTATTCATTGATGTGATGGGCTGCGACTTCGATGATGTCGAAGCCAAGCT10.8130081300813009No Hit
ATCTAGGATTTTGCCCAGGAGGTCGGGGAGGAAACCAGGCCCGACTACCT10.8130081300813009No Hit
TTCCTGCATCATATTGTCCGCGGACAAAGAATTGATCCACTTCATCTTTC10.8130081300813009No Hit
TCCCTAATCTCCCCTTCCCAAGCCTGCACGAAACTGAGCAGCCAACCCCG10.8130081300813009No Hit
CCCCGACAAGGATCAATTTTCGCGTCCGGTAAGACGGGCGATGGCTTTTT10.8130081300813009No Hit
CTAATAGATATGCAGTTCGAACTGGTGGAAATTCCCGTCGGGAAATGCGG10.8130081300813009No Hit
GTCTTAAATGGTGTAACCCAGGAGGGGTCAAGCCTTAGCCGAGAAGACGA10.8130081300813009No Hit
GCACAAGATGGACCGGTGGTACGGTCCGCTCCCGCTGCACTCACTCGTCT10.8130081300813009No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)