FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n02_Tr4_C15I1b_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n02_Tr4_C15I1b_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76
Sequences flagged as poor quality0
Sequence length251
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTATAATATTTTTAAACCATAGAGAGTTATCTAAATACCAATCAATTG11.3157894736842104No Hit
TCTTATACGTGGCACCCCCAGTCGGAGCGATTCGGCTGACTTTCCCGTTG11.3157894736842104No Hit
TGAGCTCGGGGGTATACGCCTCCTTTGATCAGCTCGCTTGGGCTCACGTA11.3157894736842104No Hit
GCAAATGAATGGATCACGACCTCTGTTTTATTAAAATGGCTTAAATCACT11.3157894736842104No Hit
GCGCCATGGTGTCCTGCTGGCCCATGTCCTTGCCGTCGAGCTGGCCGGAC11.3157894736842104No Hit
ATTGGTATAGCGACCGAAACGCTCGACTGTCCATTGATAGCCCTGGGGCA11.3157894736842104No Hit
TGGTGATGATGCTGCCGTAGAACTCCGGCGAGGCGTCGAGGTTGTGGTTG11.3157894736842104No Hit
CTTCACACCAAACGAAGACGGTGAAGATGGCGAATTAGAGCCAATTGAAA11.3157894736842104No Hit
GTTGAATATCAGATCGGCATGAGCCGTCTTACTGATCTGCTTCCAATTCA11.3157894736842104No Hit
GTCGTCGGGGTTTGTGGTTTTATAGCAAATAAGTCTTCTACTCTCGGCCA11.3157894736842104No Hit
ACTGGACCTGGGATCCGGTAGCCGGCCAGTATTTCTGGCACCGTTTCTAT11.3157894736842104No Hit
ACCGTTTTCCTTTCAGATCAAATCCTCGATTACCACGTCGTGATAAAAAT11.3157894736842104No Hit
GCAGTAGGCATCCGTCAGGAAGATGACAGTGAACTGGTCACATTCACCTT11.3157894736842104No Hit
GCGTTTCAACTCTCCGCGCTGCTCAAGCTGCTGCACGAAATCTGTCTCTT11.3157894736842104No Hit
GTGGGTAACAGCCGGTTCCTTGAACAGCGCGTCGTAAACATGCAGAAGAG11.3157894736842104No Hit
TTCATATAGAGCTTCATACATATAGCAGCCGCTATGAGCACCACATTCAA11.3157894736842104No Hit
GTTGTATTTCAAAAAGCTTCAATAGTTTCTTCACATAAAAAGGGGCAGGT11.3157894736842104No Hit
ATCAATAACTCTTTGGAGGCATGGTTATGTACAGTTCATGCAGGCAACAT11.3157894736842104No Hit
CGTACTCTAACCCCTTGCCTAGTGCGCCTACGTGCTTGGCATTGGCGCCA11.3157894736842104No Hit
GTCATGTTATGATATAGGTATTAGAAACGCTTATGCTTTTTTTGTCGGGT11.3157894736842104No Hit
ACGCAATGGCTATGAACTGGCGCAGGCGATTCGCGACGAAGAAGCCGCCA11.3157894736842104No Hit
CTACGTGAGTTACGGCTTGATCGCGCGCGAAGTAGAGCGTGTCGATTCCG11.3157894736842104No Hit
GGATTTGGTTCTTCGTGAATTCGGTAAATGAATGGAACATTCATCCAGCG11.3157894736842104No Hit
CATTTTGCCAAGATGATGCCCATATTTTTTTAAGAGAAGATCAAATCGAA11.3157894736842104No Hit
GGAGTAGTTTAGCTAATGTAGATGCACGGTGACCCTTCTGAGCAGCGGCG11.3157894736842104No Hit
TCCTCAAGGAGGTCCACACCGACCGCTTGGCCGTCACCCTGGACAACTAC11.3157894736842104No Hit
ATTCAAGAATACAGCCATGAATACAGAAGACCTCATGGTGTCTACCTGTC11.3157894736842104No Hit
GAGCTTGAAGAATCCTCTGAGTTTCCCTCCGCAGGTGTTGCGCCGTCAAC11.3157894736842104No Hit
GTCTTCGATGAAGCCCTCGGCATCGGCGCTCATCGGCAAGAAGAGGGCCA11.3157894736842104No Hit
ATACCAATCCTGCGGGCACTCGTGGGGACGCATAGCTCAGTTGGTAGAGC11.3157894736842104No Hit
GAACAGCGCGACCGATGGGGCGAGCCCCAGCTCGCGCACCGCGTAGGTCG11.3157894736842104No Hit
GAATCAATGTTTGATCAATCAGTGCTGGCTCATTTCCTAAAATATGATCC11.3157894736842104No Hit
ATTCTTAATAATCTCAGGGACCCGGAATGCAATGGAACGTATCGTCGTCG11.3157894736842104No Hit
CCGGTGGCGATGATATGCGCCACCAACGGGCCGTGCGCCACCGCCACGCC11.3157894736842104No Hit
CCGGATCGCCATGGCCGTTCTCCATGAGCAAGGACCGGACCCGATCGGTC11.3157894736842104No Hit
TCTTCATATTAAACCAATTGCCAGCTTAACAGACGGCATATACACACCAG11.3157894736842104No Hit
GACCAAAGCGCAGATCCTGGAAAAAGAGGGTCGCCTGCCGGATGCGGTGA11.3157894736842104No Hit
ATCTAGTATTCGATCAAGCTGTACACCTTTATGGATACTGGTTAATTCAG11.3157894736842104No Hit
CTAAGGAAGATGGCTTATACCTTTCCTTTTGAAAATAGATCTGTGGTGGG11.3157894736842104No Hit
ATGCTGGTCCATGGTGTAGGCACCGGACAGCAGTTCCTTGAAGTTGGACA11.3157894736842104No Hit
GCATATATCCTGACGCGCATTGGCTACAATATATTTACTACCTTATATGT11.3157894736842104No Hit
GCACTTATCACCATCCCGTTATTGACCATACTATTCTCCATATACGAAGT11.3157894736842104No Hit
GATTATCGCTACCTGCGTAACGACGGCAGCCTGGCCTGGTTCCACCTGAC11.3157894736842104No Hit
GTCCAATTAGAGGATCCTAATATTCCACTCACGGCCCCGGGTTTTTTTTT11.3157894736842104No Hit
CTGAATGACACTGGCTCCGGCATATCCTCTGCTGTGAGAACGGCATGATT11.3157894736842104No Hit
GGATCGGGCTGTAGACTCGCGGGGTGTTGCGGTACGGGACGTGGACCAGG11.3157894736842104No Hit
AGCCCGTTCCGATTACATGGTCCGCGCCCGCGAAGACCTGGCCAAAGCCA11.3157894736842104No Hit
GTTCAAAGTTTGCTTTTGCATGGGAAACACTTAAAACGTTTGATTGGTGC11.3157894736842104No Hit
GCTGAACGAAGGGCACGAACACTTTTACCTGAGCCTGCAACACCGGCCTC11.3157894736842104No Hit
ATCGCTCAGGTAGGCACCATCTCGGCCAACTCCGACAACTCCATCGGCGA11.3157894736842104No Hit
CACATGAAAAAATGGATGATGAACATCATTTTGTCTCATCGGATTCATTA11.3157894736842104No Hit
GATGGAGGGTGTTTGGAGGGTAGGCGTCTTCTTTTCGTTCGCCGAGGCTG11.3157894736842104No Hit
TTTTAAAATAACTGGCTCCGTGATACGTATCAATGAGCCAGCTTAACTCA11.3157894736842104No Hit
AGTTCACGCCATGGGCCAGAGCCAGGTACATCAGGCAATAGCCGTGGCAC11.3157894736842104No Hit
GTCTATAGACGCCTGAACATCTTTTTGGTTCTCAGCAATTAAATAAGCTG11.3157894736842104No Hit
GTACTGGGCTTCGGTGGTATCGCAGGCACCGCAACCGGTATCGCCAAAAT11.3157894736842104No Hit
TAAATACGCACGGTGCAGCAGCCAATTGGGTTGCTTCATCATGACACCTC11.3157894736842104No Hit
CGGTAGCATAACCAGATCGTCATACAGGTGAAGCGCTTCTGTCGCGATGG11.3157894736842104No Hit
GAGTTCAGGACCTTTCTTTCAGTATGGTCAACAGCCCAGACAACGGCCCA11.3157894736842104No Hit
GGACAACCTGGGCATTAAAGACGGACAGCTGGTGTGGGTGCATTCCCGTC11.3157894736842104No Hit
GACAGGAACAGCGTGGCCAGCTGGTTTCGCTGCTGCCGCGCGCGCAGTGC11.3157894736842104No Hit
CCTACTGGCCGCGCATCAAGGAAATCCTCGCCAAGTACGACATTCCGTTC11.3157894736842104No Hit
CAGCTGGTCCTCGCTGCTTGCGATTGACGGTATTGGCGTGCTTTCGTGGG11.3157894736842104No Hit
GTTGTACGTCCTGGATCCGGTGCCGAAGGGGCGGCTCGCCGGTTGCATCC11.3157894736842104No Hit
GACATAGGATGTCCTTCGCTACATCCACCCCGCGCCTAGCTGCCCTCGTT11.3157894736842104No Hit
CCCTTTGATCTACAATGTCGCCTTGCCGCCAAAAAACGGATTTTAAAAGC11.3157894736842104No Hit
TTCTCATAACCTACCAGATACAGCCTCCCCACCTTCTACCTATTTCCTAT11.3157894736842104No Hit
GGGCCTCAGTTGTATTGTTGTGTAGAGCAATTTCAATTGAAAAATATTCA11.3157894736842104No Hit
GGCCATGGCGTTGAGTCGCATCGTTTTATCCTTGACGATTGAGGCCGTGT11.3157894736842104No Hit
ATCCCCACGTAAGCGAGACGGCGCTCTTCTTCCATCTCGGCATCTTCCAT11.3157894736842104No Hit
ACTTACTGCCGCTCTTAATTGGTTACACAGGCGGTAAACTCGCCTATGAC11.3157894736842104No Hit
GGCTGCGTCCCCAGAATCGTCACCCGCTCGCCATACCGCGAGTGCGGGAA11.3157894736842104No Hit
TTTCTCTAGTTCAACACATTATGCACCCCAGACGGCCCTCACGCTCCCCC11.3157894736842104No Hit
ATGAAGAATGGCATGAGCGTGTGAAGTCATCTGCACAATGGTTAAAAGAA11.3157894736842104No Hit
GGCATGAAGCGCTCCGCGGAACCGGCACTGGTGTTGATCGCGACAATGGA11.3157894736842104No Hit
GGTTGACGTCGCCGCGCAGGTGCTTGGAGGCCATCACGTCGTATGCTGTC11.3157894736842104No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)