FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n02_Tr4_C14I4c_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n02_Tr4_C14I4c_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71
Sequences flagged as poor quality0
Sequence length251
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGGGATAGCGGCGAGTTTATTCCTTGATAAAAAATTTGGACTTTTGA11.4084507042253522No Hit
GATCGAAGAAATGCGCGGCCAGGGCTTCCCTATCGCTTACGTCGGTGACG11.4084507042253522No Hit
CAATTAAATCTCAATCCACAAATCAATAATAACCCGCACCCCCTCATTAT11.4084507042253522No Hit
CAGTGATAACACGCCATCAATCACCCGAGGGTCGGCCAGTACGCGTTGAT11.4084507042253522No Hit
ATAAAGAATCTTCTTGGCCATAGGCAGGCCAACCAATGAGAGCAGCTTCC11.4084507042253522No Hit
GAATAAGAGACTAAAGAAGGTTTACAGAGAGGTAAAGGAGATCGAACCGC11.4084507042253522No Hit
GTTTTATACAGGGGATTGGAGTAGGAGGAGAAGTGCCAGTAGCGGCCTCA11.4084507042253522No Hit
CGGTCAAACCGGGGCAGGGCAGTGACGCCGCCGCGGCATCATCGAGCGCG11.4084507042253522No Hit
GCTGGGAAACAAATCAAAGTGTATGCGCCAAAAGGCGTGCACGTGTTTAT11.4084507042253522No Hit
CTTGAGGACCGCTTTTAGCGCGGCGCGATCCTTCTGGATCGCACCCGTCA11.4084507042253522No Hit
GGTGCTGGCTGATGACGTTCTGGGTGATGGCCAGGCCCAGCCCGGTACCG11.4084507042253522No Hit
GGTCCGACTGGTCGAAACCGCTGACCGCTTCGTTGAAGGTGATGGTCACC11.4084507042253522No Hit
TCACCACCCTGAGCCTCATGCCCGTGCAGCACCGCGCCGACGAACAGCGC11.4084507042253522No Hit
GGTAGTAGCAATCGTTGTTGCTCATGCGCTGCAGGGTGAAGATCCCCGAC11.4084507042253522No Hit
ATCATCGAGTGCCCACATGAGGTCGAGGCTTTGGTTTCGGTATTCAAAGG11.4084507042253522No Hit
ACCATACCGTCGCCAAAATACTTCTTATTCAGGTCGTAATACAGGTTCGT11.4084507042253522No Hit
ATTCTGAGCCTGGCGCGGCGCGCGCGGGCAGGGTCCAACACCCGCCCGCG11.4084507042253522No Hit
TGTCATCACTTTCTTCGAACTGACACAATTCCGCCGCATTCTGTCATCCG11.4084507042253522No Hit
TCAATATCGTGCCGAAGTTGCTTTATATCTTCTTTCATTCCGTCTACATC11.4084507042253522No Hit
GCTGTTGCTTTTAAGTGAAATACGCCAGAAACCGTATCTCCTTCTTCTAC11.4084507042253522No Hit
ACATGCTGCTCGACAGCGCCAAGCAGGTCGTGGCGGCCGCAGACCGCGCG11.4084507042253522No Hit
GTTATTGGACGGAGGATCATGGTAAACAGGCGCATCTTCATAAGCCCTGT11.4084507042253522No Hit
GAAAATGATAGATCGTTTTTTGGACCGACACTTACGAACAAACTGCATGA11.4084507042253522No Hit
GTCATGAATGCACCGGATCTGAACCTGTTGATCACCCTCGACGTGCTCCT11.4084507042253522No Hit
AAAGAAAACAGGTGGATAATCGATCCACCTGTTAGATTGTTGACAAAGGG11.4084507042253522No Hit
CTTGCATCTGTTGTCCCATTACAGCTAATCGCTTACTACGCAGCCCTTCA11.4084507042253522No Hit
GTGTTCGGTTGTTCCAGCGTATCGAGCAGGGCCAGGGCTTCTTCATAACG11.4084507042253522No Hit
TTTCAAGGCCAGCGAACGGATCGTGAATCGCAATGCCCTGCGTGGCGCCA11.4084507042253522No Hit
TTATGAACGTTTTGGGCTTCCGTGCCCCAGAACTTTGCGAATCCCGTGGT11.4084507042253522No Hit
GTGCCGAGCCGTATCGGGCGGTGCTCAAGCAATTGCGTGAACGGCTGCGC11.4084507042253522No Hit
GAACGGCATAGCGGTCATCAGAAAATTGCATCGATACATTCTTAAATTCA11.4084507042253522No Hit
ATATCACAAATTGTTGTCACATTGTGAAAATCAATAAAAAATGCTGCTGA11.4084507042253522No Hit
GACTAAATAAATTCCAGCCATGAGACAGGCTCTTAACACAGACGGTGCAG11.4084507042253522No Hit
ATCATGTTCATGAGAAAAAGGATCATAAAGATCACAAGGACCATTTTCAT11.4084507042253522No Hit
TCTTTGTTATGTCTCGGGGTCGGTTCATAAGGTAGTGCTGCCGTTTAACC11.4084507042253522No Hit
CCACACCACCAGACTGCCGAGGACCAAGGTCATCAACAGCGTCGGCCAGC11.4084507042253522No Hit
GGCTGTAATCGCCGTTGGCGCCCTGGCTGAGGCGAAACGGCCCGCCACGC11.4084507042253522No Hit
ATTTCCTCTGCTCTTTTAGTCTAATTCCTTTTTTTCTACTTTCTCCCTCA11.4084507042253522No Hit
TCATCAGTCTCTCCTCCAAGTATGATCATTGTTTGAACTGCTTCTATTAT11.4084507042253522No Hit
GAGAGATCCGCGCCTTCGATGCCGAAAAGGGGGGCTTCCGCCCGCTCCTG11.4084507042253522No Hit
GTCCGCAACGATCAGCGCGCCGCTCTTGTCCAGCGCCACGCCCACCGGGC11.4084507042253522No Hit
ACCTGGACCTGGTAGGCCTCGAACGTGTTGAGTGCGCCGACGTAGGTGGG11.4084507042253522No Hit
AGTACGCATTAAGCGCCTTGTTCTTCTTTTTGATCTGCAACTGCTTTAGT11.4084507042253522No Hit
GAACTAAAGCATGTGAATGAAAAATGGATTCATGAACCGAGTAAAATGCC11.4084507042253522No Hit
GGCCGAGGTGAAGAAAGCCGTATCTCTCCGCCGTCTCGCGCACGCCGCCA11.4084507042253522No Hit
GAACTATGCGGAATTTGGGGATCAGCCAGAAGTTCTCAAGCGTGAAAATG11.4084507042253522No Hit
GGAGTGATTCGAACACTCGACCCCTAGCACCCCATGCTAGTGCGCTACCG11.4084507042253522No Hit
ATCCTGAGTGCGCTTGGCGAGGTTACGGACTTCGTCGGCCACCACCGCAA11.4084507042253522No Hit
GCTGCAATCGCGCGAGACGATCCTGTCGCCGCGTTTTGCACCGGACGGCA11.4084507042253522No Hit
TTATGGAGGAAAAGCGTACCGGATTGGAAGATATAGTGTAGACCGTCTCT11.4084507042253522No Hit
GTTTTCCGAAATCTGGTGTCGGTTGGCGATGAATCGTTTCGCGCAGCGTA11.4084507042253522No Hit
ACGTATTTAACGGGTCGTATCGTGTTCTATCGCTTCTTCTCCCGTTCTCC11.4084507042253522No Hit
CTCAAAATTCATAGTACATCGGGTTTTTGAGTTTAATAAATTATATTAGA11.4084507042253522No Hit
CATTTATATCACTCCAATAATGTATTTTGCAGAGAAAAGCAGAAAAACTG11.4084507042253522No Hit
GTCAACGACCTGACCCCGGAGAAGCGCAACATCGCCCAGGTGTTCCAGTT11.4084507042253522No Hit
TGATTATATTGAACCTTCACTAACTGGCGGCAGCGCCAATTTAAAGGTGA11.4084507042253522No Hit
TTTTCAGGTAGATAAGTCCACTAATCAAATACATGACAAGATATTAGCAA11.4084507042253522No Hit
CGGCCTGACGACGGCGCCGTTCTGGCTGGCGCTGGCCGGCGTGGTCCTGT11.4084507042253522No Hit
GAATATTGCCGCAACGCAAAACAAATCACTTTTGACATTGCGGTGCAGCA11.4084507042253522No Hit
TATTCATTGGCAGCCTGAAGATATTGTGTTCTTAGATTCGAAGGTGCATG11.4084507042253522No Hit
AATCACTATCGATTACTTTTTGAGCTCCCTGGCTATCGCGTTGGACGACA11.4084507042253522No Hit
GTACTCGTCTTCGCCGGTGCGCAGCAGTTGCCGTGCGCTGATGCCCAGCT11.4084507042253522No Hit
CTTCAAAACAGCCTCACCCCATCGGGTGGGGCTTTTGCACATTCTTGATA11.4084507042253522No Hit
AGGCGAGGGCGTCGAAGTCGTGGGCGAAGTCATCCATGTCCGCCGAGGCC11.4084507042253522No Hit
CAGCTATTCAAATCACCCTTTACCCAAACAACACCTGAGCAATGTTCAAA11.4084507042253522No Hit
AACTACGAGTGGGCACTTTCCATCAAGCCGACCGACTGCGCCAGACAAAC11.4084507042253522No Hit
GATCTGTCCCTTGTTTATCCGCTTGTTGTAAAACGCTGTCTCTTATACAC11.4084507042253522No Hit
CCCGAGGACTATGCAGCCAAGCTCAAGCTAATCCTTGACGCAGGCGAGGC11.4084507042253522No Hit
TTTTTGTGTCGTTCATATGGTTTGTGAACGATACAAAACCTTGTGGGAGC11.4084507042253522No Hit
CGCTGACGCTGGCCACCGGAAAGCTTGACGCCCCTGCCGCCAACAAAGGC11.4084507042253522No Hit
TGTGGATACAGGTCGCGGGCCAGTACCACCTGGATCTTCTGGCTCATGTA11.4084507042253522No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)