FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n02_Tr4_C14I3c_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n02_Tr4_C14I3c_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83
Sequences flagged as poor quality0
Sequence length251
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCATATATCTGCTGCCTCATGCGGGTCACCACCTCAACCAGCCACAGC11.2048192771084338No Hit
GAATAAATTCGAATCTAACTTAGAACAGCTAAAAGAAATTGAAGTCAATG11.2048192771084338No Hit
ACACTCATAACCGGATCGGCTAAGCGAGGTCGCCACTCTCGGACGCCTGG11.2048192771084338No Hit
GCTATTTGCTGCGCGCCTCAGATCCTTGCAGCCGTTTCGTTTGTCTCTTA11.2048192771084338No Hit
ACTCCATTGCGGCTGCACAAGCAGCAGCGCCACCAGCAGCACAAAGAGAA11.2048192771084338No Hit
TCCAGGTGCTGTTCGACCTGACGGTGATGGCGGCCGTGCTGGCGATGCTG11.2048192771084338No Hit
GTTTAAGTCTTGTCGCCTTCGAGGACGCTTTGACTCAGGCCTTACCACAG11.2048192771084338No Hit
CCCACAGTCCGGGCGACGTCACTCGGACAGATCGAAGACTCGCCCCGCTT11.2048192771084338No Hit
CAGGTTACGCGCCGTGTTGGCAAGCTGGCTGCGTACATCCGTCAGCTTCT11.2048192771084338No Hit
ATCCAGGCTTTGACGGTGCCCAGCGGCGTGTCGAGGCGTTGGGCGATCTG11.2048192771084338No Hit
TTTACCGCCTGCCCAAGGAGCGCTCGCTGCTCGAGGTGCAGCGCAACTAC11.2048192771084338No Hit
CAACTGCGCGCCTGTTTTCGCCCCGCCGTCGCCCTGGCTGCCTCCGCGGG11.2048192771084338No Hit
TTCCCATGGCGTGCTCAAGGCCTTGATGACCGGCATGCAGGCCGCGCAGG11.2048192771084338No Hit
GGCAACAACTGGTCGAGCGCCAACATTCATAACCTGATTGCCGGCAGCGA11.2048192771084338No Hit
GTTGATCTACTCGCTGCTGCACCTGACCGGTTACGATCTGTCGCTCGACG11.2048192771084338No Hit
GCTACACCGTCCGCGACGCGAACACCCTCGCTGCCCGCGGGCGGCAGGCA11.2048192771084338No Hit
CCCTCCCCCGCTGGGGGAGGGTTGGGGTGGGGGCACGGCGGCGCTCGATG11.2048192771084338No Hit
TTCCTATTGTGGGCATCGGCGGGCTTACGCATTGAAATGAGTCACCAGGT11.2048192771084338No Hit
CCCAAAAATCGAGTGATCATTCTAGGCTTGCCCGCAAAGCCCGTCGACCC11.2048192771084338No Hit
GATCCTCGAAACCATCGCCCGCAGCCTGTTCCGGCCCAAGCGCTGGCGGA11.2048192771084338No Hit
GTGAATCCCGGTACGGTGTGGAACAAAAATAACGAACGATTTCACTTGGA11.2048192771084338No Hit
TTCCCGCCCAATTATTTGCACGAAAGCTGGATGGACTATCTGTACTGGGA11.2048192771084338No Hit
GTATGAGGGTGCGGATTCGCTCGGCCTGAACTACTGGCAGAAGACGCGTC11.2048192771084338No Hit
GTAGAAGACAGCGGCGACATCGACGCCGCCCTGCACGAGTACGAAACGCT11.2048192771084338No Hit
ATCCTGGGCCGGGCGCGCGTCTGCCACCTCGACAGGTTCGCGGGCGTTGA11.2048192771084338No Hit
AGTTCGCTGGGATGCTGCATATCCGCAACTCGACTGCCCGGAGCAGCGAC11.2048192771084338No Hit
CTGCTGATCACGCTGGCCCAGATCGTGTGTGCCGTCATCGCGGTGTACTT11.2048192771084338No Hit
CCTTTAGCTGCTTCGGCAGAAGGTGATCTTTCACAATTTCTGCTTTTTCA11.2048192771084338No Hit
ATTATGTGTGCCTTGAAGGTTCACTCAAACTAAAAGAGATCTCTTACATC11.2048192771084338No Hit
AGTCTGCCTTCTGCCGCTGACCGACGACACGCGCGGCATCCTGGATCCCG11.2048192771084338No Hit
CCGATGGGCGAAGTAGATGCCCACCGGCGAAAACACCTCGCCGTGCGCCG11.2048192771084338No Hit
GTTGACGTACTGCGAGGAATCGATGCGACGGATGTCGAGGTTGATGCCGA11.2048192771084338No Hit
TTGAAGAAGTGTATCAAGACTTTGGCTTGAATGACTACACATTCCGCTTA11.2048192771084338No Hit
GTTTAAGCATTAAACCCGGGCTCCAGCTTCGACCTGCTCGCTCGTTTTAC11.2048192771084338No Hit
GTTCGACATGCTGTTCTCCACGGCCGACTGTCGGCCTTCATGTGGCGATT11.2048192771084338No Hit
TTACATATCCATCCTCACATGTTAAGGCACACCTTTGCTACGCATCTTTT11.2048192771084338No Hit
GGCTTGAGTACACCGGGAGGGTCACCTTGGTGGTCGGGGCATCGGGGCGC11.2048192771084338No Hit
GTGTACAGCCGATCTTCGGGATACAGATGCCGCAAAGAGTCCGTCCAGCC11.2048192771084338No Hit
GAGCACTCCTGCGTGATCCGGAGATTTTATTGCTTGATGAACCTTTTGGT11.2048192771084338No Hit
CTACTGCTTCTCTATTGCCATCATCACATAAATAAATTTGAACGCTTTCC11.2048192771084338No Hit
AAAGCGTTGTAATGACTGGACTGGGAGTTAATTCTCCACTTGGAATTGAT11.2048192771084338No Hit
CGTTTTGTGCTGTCGAGTACATTTTCTGCCACTTGAATGGTAATCTCATT11.2048192771084338No Hit
GTTTCGAACAGCGCGCGGTGGTTTTCCCAGCTCGGGTCGCTGATCGCCAC11.2048192771084338No Hit
CATTGGTTGTTCACCCCGTCGTCGTGACCACCCTCCATCACATCTAGGAC11.2048192771084338No Hit
GTTTTTCTTGCTCAGGAGAGAACGGCATGGACAGTTCATCATCAGGCGCC11.2048192771084338No Hit
ACGCTGCCTTCTAGATGTCGGCGACTCCAGCGCCCACCCTCCCCCACAAG11.2048192771084338No Hit
GCACAAGCCCCATGGCCACCATCAAGGACGCCGCGCCCAAGGCGGAGGCT11.2048192771084338No Hit
TTAGAGATGCACACGGATGGCGCATAAACTGGCTGCCGCACATAGTTTAT11.2048192771084338No Hit
GTGTTAGGCATGCAGCCAGCGCTCGCTCTGAGACAGGATCAAACTCTTAA11.2048192771084338No Hit
CATCAGCTCGACGCTCAGAAAGAGTTTTTCTAGGCATTTTTCATGCTCCT11.2048192771084338No Hit
CTTTTCGCCCAATTGATAGCGTTGAGCCTCGATGCCCAGAATAATCTCGA11.2048192771084338No Hit
CGAAAAACATATTATTTTTAGACAAGAAAACGGCCAGACAAGTGAAGTCC11.2048192771084338No Hit
GGAAGCTAGTCGGGTTAGATCGACGAAGGCTTTGCGGAGGGCGTTCGTCT11.2048192771084338No Hit
ACCAATTTCAATTCCCTTTGCGTGTGCTTCAATTCGTTTTCTCACCCTTT11.2048192771084338No Hit
GGCGCGCGGAGCTGTGCATCGGCCCGGGGCCGACCTTGTCGAGTGCCTCC11.2048192771084338No Hit
TTCCTCCACCTTCGTGTGTGACGTCCTCCCGCTTTCGGCCGCTTCCGCCA11.2048192771084338No Hit
GAGACGATGGATTGCACATCGCTGATGTTCAGCCCGTAGCGGCCGGCGGC11.2048192771084338No Hit
GATACGCTCAATGCTGGCGGGAGTTATATTGAGCACATTCCCGCTCAAAA11.2048192771084338No Hit
GCGGAAGAGGATCGGCCACTCGCGCGGGTTCTCCAGCGCGTAGTCGATGC11.2048192771084338No Hit
GCTTCCTATGGCCCTTACGCAGCTGGGGAGACGTAGAATGCCTCGAAAAA11.2048192771084338No Hit
GATGAAGCTGGCCGACCTGTCGACCATGACGGTCAAGGCCCAGGTGTCCG11.2048192771084338No Hit
CGGATCGGCCGAAGCTGCCAGGCTAATCGCAGCGCCGTGGGATCTGAATA11.2048192771084338No Hit
CCTTCCTTGTCTTTTCGCTGATGCCGGTGCTTCCGCCCTCTCCCCCGCCC11.2048192771084338No Hit
ACGCAGATCATCGGGAGCCAGTTCGGCCAGGGGATAGAGAACGAACGCGC11.2048192771084338No Hit
AGAAAATCATTCTGCACGCACGCCGCCACAACAAGGCGGTGATCGTGGCG11.2048192771084338No Hit
CAGTCGGGCCGGTTATTCCTCCACACATATTAAGTTGGGCGCACATGTCT11.2048192771084338No Hit
ATGGGCGTTGGCAAACCGGAAGATCTGGTTGAGGGTGTGCGCCGCGGTGT11.2048192771084338No Hit
CGACGAATACATCATTGATCAGTTCAATCAATTCTTTGTCGCAACCGTTG11.2048192771084338No Hit
TAACTAACGTATGGGGGGGGGTGGGAGCCTGTGGTTGTTGACATAACCGG11.2048192771084338No Hit
GGCTTGGTTTTCGGTCATTTCTTTTTTCTGTTCAGTTCCGTTGTTCGGGG11.2048192771084338No Hit
GTGCGGAGGTGGACACGCCGCACTGTATGCAGGCAGGCGCGTCTGCACGG11.2048192771084338No Hit
CAGCAAAACCAGCATAGGCCGTTCCCGCATTGTTCACAAGCAGGTGAATG11.2048192771084338No Hit
GGCGTGGGCAGGGTCTCCGAAAGCGGTGGCGGAGCATCGTTCACCTGCGC11.2048192771084338No Hit
AACCATGGGTGATCGGGATACTCGACGGTCTCAGGAAGCTGTCTCTTATA11.2048192771084338No Hit
TAACAGACATATATAGGGCCGTCAGTCGCCGGTGTTTTGGTTGTAGGTCG11.2048192771084338No Hit
GACCAGCACTTCTTCGATGTAACCGGCGACTTTCGGCCCGATCACCGTGA11.2048192771084338No Hit
TGCTTTTCCTTCAATATCAGCAGCTGCAAACCCTTCATAGTCTTCCACAT11.2048192771084338No Hit
CTGTCTGGTCTTCAAACCACAAACCCTCTCCGAGACATCGGGGAGGGTTT11.2048192771084338No Hit
ACACAATGTCTTCCATTAATCCTGCTTTCACTGCATACGATTCAACCACT11.2048192771084338No Hit
GGATAAATAATGGCGCATGCGGATTGATGAAATGCCGCAGCACCGCCAGC11.2048192771084338No Hit
ATCCACATGCGCTGTGGATAGCTGAAAAATCCCCGCAAAATCCGGAGATT11.2048192771084338No Hit
CTGCAGGAACACGAACATCACGATGACAACGAGGATGATCGCCTCGAACA11.2048192771084338No Hit
GCTTCTGTCATCGGGCCTCAATCTGATGGGTGCCAACCAGCATCTGGCAA11.2048192771084338No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)