FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n02_Tr4_C14I1b_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n02_Tr4_C14I1b_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42
Sequences flagged as poor quality0
Sequence length251
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTTAGGGCAAGGTTTTTCCCCATAACGGCTAGTCCAACGACACCGATT12.380952380952381No Hit
TTCGCGGCTGATGCGCGCCTGAGCCGCCTTCGACAGGTTGGCCGGTCCCA12.380952380952381No Hit
CCACTGTTGATCACCAGCCTCACCATTATCGTGGTGATGCTGGCAATCGC12.380952380952381No Hit
GCATAATCCTTCACTTCATCAGAACGTCTTGCATACGGCTTTTGGAATGG12.380952380952381No Hit
GCACCTCCTCCAGGCTGACACCCGCCCGCACGGGTGTCGCTCCAGTCCTC12.380952380952381No Hit
TAAGTCGCGTTTCGACGCTGCTTTTATGCCTTCTAATGCCTCGCATCCTC12.380952380952381No Hit
CACCCAGGCCCGGTTCAACAATGGGAGATTTGCCAGCGTTGATCATGTCG12.380952380952381No Hit
CATCAGAAAGGTGTTTTTTTCGGGTGCCTCGGACAACGCATAGGGAAACC12.380952380952381No Hit
GTGTAATATATATGCTCAAGAAACTCATCACAGAGAAGCATTGATTTTAG12.380952380952381No Hit
AAATATAACGGCAGCAATAAAGTATCCTTCTAATACGCGATAGTATAAAG12.380952380952381No Hit
ATTGATGGCCGTCCATGCCCGGCATATTGACGTCACTGACCACCAGACTG12.380952380952381No Hit
GTTCCTGGTCGTGCGTTTGGGTGTGCGTGTGCTTGGTCATACAGCTGTCT12.380952380952381No Hit
GGCCTGACCATCCGCCATGCCGCCCAGCATGCTGCCCTTCAACTTGCTGG12.380952380952381No Hit
TCGTGAAGACCAGCGCGTAAAGAATACTCCCAGACATTTTCATCCACTTT12.380952380952381No Hit
GGGTGGCACGTGGTTCAGGCTCGCCAGCAGCAACACCGCGCCCTGTTGCG12.380952380952381No Hit
TTTCCAGCGTGTCCTTGCCGTCGGCCGCTGGGGCCCGCCCTTCCGCGCGC12.380952380952381No Hit
CAACTGAGGTCCGCCATGACTGTTTCGAACAAACTCTCACGCAGCGAAAT12.380952380952381No Hit
CTCCTCGGTTTCTCCGTGCCCCCTCCTTCCCCCCCCCCCCCCCCTCCTCC12.380952380952381No Hit
GGTGCAGTCTAGAGGGTATGGCGGCTTTTCTTCAGACAAATAAAAAACGG12.380952380952381No Hit
GTTTAAGTTTCAGATCGTGCAAATTGTTACATTGGTCGGTGATGATATTG12.380952380952381No Hit
CTACACTCGATGATCTCATGACGAGTATGCCTGATTACCATTTCATCAAA12.380952380952381No Hit
GTGTTGGGTCGAACCGGCCTTACCGCAAGACCACATAGCCTTCCTGCAAT12.380952380952381No Hit
GTTGATCCCTCTCTGCAAGAGCGGCTAAACCGCCCCCACCCCCCCCCCAC12.380952380952381No Hit
GGATGTTGGTTCTGGCGTTGTCACAACGACCACCTCTCCTGCCGATCTGT12.380952380952381No Hit
TGCACAGCTGAACAGCTGCATCGGCGAATGCCAGCGCCGACTGCGTCACG12.380952380952381No Hit
AAATGAGAGAAAGTAAAAAACTCCGACGAAAGCCCATCGGTGATGTCTGC12.380952380952381No Hit
GATCAGGAAAGACGCGGACCGATGGGCCGCGGTGGTGAAGCGCGGCAACA12.380952380952381No Hit
TTTTTATTAATATCTTCGCTTGATCTGCTGCCCTTTAGCACAATACCATC12.380952380952381No Hit
CCTGAGCAACGGGCGCATGCTGGCCGACTGGCACGATGCGCGGCTCTTTG12.380952380952381No Hit
GTGCTAGGTATGCTCCATAACTGCTTACCCCGGATTGATAGCCGGTCTTG12.380952380952381No Hit
AGATTTAGGGATTCAGTATCATTTGTCCTTATTTCAATTTTATTTATTAA12.380952380952381No Hit
GCTGAAAAGCCTGGCGGTGCACCCGGTGCAATACGCGCTGGTGGGTGTGG12.380952380952381No Hit
TGATGATCGTCTTCAAAACATTTAGAGGCCGTCTGAATGTCGATCAATTG12.380952380952381No Hit
TCAAGCTCGTGCTGCACGCGATGCACGTCAACGAGCTTCCGTTCATCAAC12.380952380952381No Hit
GCCTTGGTTTTTGGCGTGATGCGCTCGATAACGCTTTCCGGGTCAAGGCA12.380952380952381No Hit
GTTATGAACGTTTTGGGCTTCCGTGCCCCAGAACTTTGCGAATCCCGTGG12.380952380952381No Hit
GCTACTATATGATCTATGAGCTTGTGCAGCTTTATGGACGAGATGTCATA12.380952380952381No Hit
CCTTTGACTGGCGCGGTGATCTTGCCGTCCTCGATCAGATAAGCCTCACT12.380952380952381No Hit
GCTGTAGACCACCGAGATGCCGCTGGCGCGCACGCCGGCCGGCAGCATCT12.380952380952381No Hit
CAACAACCACTGTCGGCACCGCCGCCTGCGCCGGTCGCAGTCTCACCGGT12.380952380952381No Hit
GGTAATTCCCGATGAGTTTCAATCTCGCCGACAAATCCCTCGCAGAGCGC12.380952380952381No Hit
TCACCGAACTTCCCTTACCCGAAATCCATTTCGCCCCTCATCCCGTCTTT12.380952380952381No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)