FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n02_Tr4_C13I3a_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n02_Tr4_C13I3a_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22
Sequences flagged as poor quality0
Sequence length251
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAACAGACTCTCAGATGAAGGGGTAGCGCATGGCCTGGTGCCCTGGCTTG14.545454545454546No Hit
GAGGACTAGGAATGAGGATAAGAAGAGAATTAGGGTGTCGGCCCATGGGC14.545454545454546No Hit
CTGTGGGAGCGAGCCTGCTCGCGCTAGCGGCCTCTCAGTGCCATGTGTAT14.545454545454546No Hit
CCTCTGCCGGCCCCAACTTGTGAAAACGGATCCTGCTGGACATCGCAAAC14.545454545454546No Hit
AAATCTCATGCACACCGATACTCAATAACAGCGCCACCAATAACCAAAGC14.545454545454546No Hit
CCTGGACACCACCAGCAGCATGGCGCTGCGGAACACATGGCCGTACAGCA14.545454545454546No Hit
CAATATCATTTTATTGCCGGAATTCTTCGTTTAAGCCTGGGTGTTCAAGG14.545454545454546No Hit
GAAACGGCGCACACACAGATGGTAGTCATAACCTGCCCTGCAGTTAGAGC14.545454545454546No Hit
GACCCAAACATGGCGTTCCATCCAACCGACAAACACCTGACCAGAACACA14.545454545454546No Hit
GTGTCGATCAGGCCCATCTCGACTATGGCCTTGTAGGTGGGCAGGATCAG14.545454545454546No Hit
GCTTTCTTCATGCCGACGATGCCGTCGGCGGCCTGTGTTGTGTAGTTCAG14.545454545454546No Hit
GGGGGAGAGGGGGTGGCGTGCGACGGCGCGGCGGCGAGCGGCCGGTGCGG14.545454545454546No Hit
TACGTGGCGACAGCAGGAAGCAGTTGTGTCAGCAGGTCCGGCGCAACCGT14.545454545454546No Hit
GGCCTTCTTCCTGATCGTGGGGCACGAAAATGGTGGTGATGCCGAGTTTC14.545454545454546No Hit
CGTTAGGAGAAGCGCGAAGCGCTTGGAGCCATTCTCATGCTGGCTACGGA14.545454545454546No Hit
TTGTTAATACGGGCTCTTCGGTACAACAGGCGTGGCTAAATGGCCATAAT14.545454545454546No Hit
CCCCTGACCTGTATCTGACCGCCGATCGAGGCGGTAGCGAACTCGCCACC14.545454545454546No Hit
CCGCTTCTGTGTCACGGTTCCAGGGGGTCACTAGCTCGGGTCTGAAGTCG14.545454545454546No Hit
ACTTAAAGGTCAGGAAAATCCGGTTCGTCGATCACCAGCGTGTCTATTCA14.545454545454546No Hit
ATGTACGATCAGGAGAGAGACGTCGAGGAATTGTGCACACGCGTCTGTCT14.545454545454546No Hit
CCTCTTTCAGGACCGGAAAATGGTTAACAAAATCACACAGCCCTCTGCAG14.545454545454546No Hit
GTCGATATAGTGCGGGACATCGTTCAATAAGGGTGGCGGTAGTGGTAGTG14.545454545454546No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)