FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n02_Tr4_C13I1b_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n02_Tr4_C13I1b_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69
Sequences flagged as poor quality0
Sequence length251
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTTCAAGTACGTGCTCGATTACCGCGACGGTGAAGTGTTCTGGTGCAC11.4492753623188406No Hit
GGAAAATAGTAAAGTGGACTAGCGCGATGACCCGCGCCTTCAAGTCAAAT11.4492753623188406No Hit
TCACTACGGTTTCACCTTCGATGAAGACGTGGCCGATGACCACACCATCG11.4492753623188406No Hit
TTTATCACCTGCTCAAATTGGTCTTCTTATTGGGTCTGGTTATATCGGAC11.4492753623188406No Hit
GCATTGTTGATGGCGAAGATCTGCACTTTCCAGCGCTGCACATCGCCGGG11.4492753623188406No Hit
GTATATGGGTCTTGGACGCTATGGATGGGCCGGCGCAAGATTGCTCCCAC11.4492753623188406No Hit
GCCATGGCGTTCAGCCTGAACGCCATGGCCGGCGCTGGCTATCTGGCCTG11.4492753623188406No Hit
CGCCCGGCCTGCTAGCCGTCGACGTCGTGCAGGTGATGGACGACGTCGTG11.4492753623188406No Hit
ATCACCACCCGCGTCGAGGCCACGTTCAACGCCAGCCGCAGCAGCGTCGC11.4492753623188406No Hit
GACCCTGAATCGAAGGTGTTCCTACCCTCACCCTAACGCCGAGCGTAAGA11.4492753623188406No Hit
CTCCCAGAGGGAGAGGGGACCGAATGGGGGATATTCAAGAGATACGCCGA11.4492753623188406No Hit
CAGCAGAACCGCGACCGCCACTACGATCAGCGGTATGCCGATAAAGCGGC11.4492753623188406No Hit
GACGAAGCGGATCGTGGATATTTGGGTCTTGTGTATCATCGATTGATTAT11.4492753623188406No Hit
TACCAATACTTGCTAGTACTGCCGTTAAGACAACTTTCAGCATTTGAACA11.4492753623188406No Hit
TAAGAGATATCTGGATGCAAGGGTCGTACTTGTGGTGAAAATAGGTTTAG11.4492753623188406No Hit
ATATTGGAGCATATCGTATGCGGTAAAGGTCAGCTTTCCTTCAACAGGCG11.4492753623188406No Hit
ATATAATATACGCTTATCCACCGTTTGCTCCATTGTCTTTTTCAACATAT11.4492753623188406No Hit
GATTGGTTGATCTCGCCCTTTTCGTAGCTTTCAGACAGGACTGTCTCTTA11.4492753623188406No Hit
CAACTATTCCAACGGCGTCAACAAGAACGGTTAACTCGACATGGGCCTGC11.4492753623188406No Hit
TCCCTGAAGAATATACAAAAAGCTTATTCAATGTCTCTGAAACATCTTCA11.4492753623188406No Hit
GCTCATGACAGGCCCCTCGCGCGGCCCGACGCCCGGCCGCAAATCCACAG11.4492753623188406No Hit
TATCACCCATCCTCTCCATTCTGAACTTCACCCCACTCCCGTTGTGTCTC11.4492753623188406No Hit
GCACAAAATACATTCCAAAAGCTTGGACTGGAAGACATTCAATTATTAAG11.4492753623188406No Hit
CCCAGAGTGTTAAGTCTTTTCGAACCGTTAAACCTGTCACGTTTTTTGTT11.4492753623188406No Hit
CCCTCGGCCTGCATACAATACCCCGAGGAGGTGACCTTCGATGAAGGCTT11.4492753623188406No Hit
GCCGTACCGCCCCGTGATGTAGTCTTCGGTCTGCTTCTTCGCCGGGTTGG11.4492753623188406No Hit
GCATCAGCCCGGCATTCGATTTCCAGCAATTTACCCGTGCGTTGCACGGT11.4492753623188406No Hit
TATGAAAACAGTACTCGAGAGCGCCCTCAAAAGTCCACCCAGTCCCAACA11.4492753623188406No Hit
ACTATCTGTTGGTGTGTGAAATGCCGGGGACCGTTTTTTTGCGGGTGAGT11.4492753623188406No Hit
GGCTTATAATGCTACTTGGGGGTCGAGTGTATCGCATTCGCCGGATCAGA11.4492753623188406No Hit
CTCTTCGTCTCCTCCTACCTGTTCTCTCCCTCCCCCTCTTCCTCGATCCT11.4492753623188406No Hit
GAAGTCGGTGGCGCTGGCGACAACTATTTGTTCCTGTCGTCGATGATCCA11.4492753623188406No Hit
TTTTTGCACTAACGTGTCCTTCTTTTTGGATTTCTTGCTTGTGCTCTCAA11.4492753623188406No Hit
GTATTGGAGCAAACTCTTATCAAGAATAAGCAAGCCGGGTTACCAGAAAT11.4492753623188406No Hit
GTTATGGTTTTCTTTCCGAAAGGGCATCGTTTTCGGAAGCGGTTGAGGAA11.4492753623188406No Hit
CTTCAGATCAGTGCGGCGCCTGACCCGCGGTATGCCAACTCGATCGTGCC11.4492753623188406No Hit
GCCAGATACTGGTCATTTGGGGCTTGGCGTTGTTGTTCATGATGCCGCGC11.4492753623188406No Hit
CATCTTGTGATAGTGCCTGAACTGTAATTGCAAGAGAATAGCCAGCGACC11.4492753623188406No Hit
GCCCTAACCCCAGCCCTCTCCCGAGGGAGAGGGAGCCAATTTGCGGGTTT11.4492753623188406No Hit
AGATTCTTGGGTTTTATATCCTTGGTCGGAGAAAGTTGTGATGAAGACGG11.4492753623188406No Hit
CGCGGGTATCACTACCACAGTTTTGTGGGCGTGCTTAAGATGGGTCGCAG11.4492753623188406No Hit
GGATTAAAGCGCTTGCGATCTCCGATGATTCGCAGACTCATCGCGCATTC11.4492753623188406No Hit
CAGTTGTATGATCCTAGCCTACATTGGCCACCGCACTCCCCCGTACTAAA11.4492753623188406No Hit
CCACCAGCCAATACCAAGTATACTGCCCTACTCCCCAACTTACCTCCGCC11.4492753623188406No Hit
CGTCTACTGCTCCGAAGCCAGCCCCGCCGGCTTCGACCCGAGCCAATACA11.4492753623188406No Hit
AAGGGCAACGCCGCCGAGAAGGCTGCGACGGGAAATGGTCAGTTCGGGAA11.4492753623188406No Hit
CCCACATTGGATTGATGTTAACTATAAAAAATGCTCCCATTTGGGAGCCT11.4492753623188406No Hit
TCGCTGTACACCGGGCTGAACATCCTCAACACCGTCGACATCAACATCTC11.4492753623188406No Hit
GGCAATGACACCTACATCGTCGACAACGTCGGCGACGTGATCAGCGAAAC11.4492753623188406No Hit
CGGTGGAGGAATCTTACGCTCCCCGGCCGTCCTACGGTGCCCCGCAGTGG11.4492753623188406No Hit
CCCATGGCCAGGATCACCAGGGTCAGGATGCCGCTGATGTAGGCTTTTGG11.4492753623188406No Hit
GCTCGACCGCAGTGCGATCTACCGGTGTCCACCAATTGGCATTGTTGCCC11.4492753623188406No Hit
CTGTTATATATTCATGTCGGGTGATACTTGAGCTGATGACTACAGGCTCG11.4492753623188406No Hit
TGTCTTTAGTACCGTCTATTGTTGGGCCCCCGTTGATGTCTGGCAGCCAG11.4492753623188406No Hit
GCTCGATGGACACAGAACAAACTTGCATAATGAACAGCTTGCCGACGGCC11.4492753623188406No Hit
GATTTTATATTTGCAGTCATTGGCGAGGAATTTGGTTTTATCGGCGCCTC11.4492753623188406No Hit
GGAAAAAGGCAGCTCACGTTTTCGAAGTGCATAAATTCGATTACCAAGAA11.4492753623188406No Hit
GACTAGGCCCAAGGACCTTCAGACCGAACTGAAGGGGCTGAGCCAGGCGT11.4492753623188406No Hit
GTTGACCAGACGATCAAGGAATTCGTACATGCGTGCGCCACGAAGGGTAA11.4492753623188406No Hit
GCTTTTTGACCGTCGAAGGCGCCGCACGTGAACAAATGGGCGAGCTGCTG11.4492753623188406No Hit
ATCATGGAGACCCTGTCCGGCCTCGCAATTGCGGTAGTTATTGCTGTTTC11.4492753623188406No Hit
CATTGGGCTAAGACAGTCTGGAATCATCACTGCTGATCCACAAAATCTTG11.4492753623188406No Hit
TTGCTACACTCGCATTAAAGTAACTGTAAACATAAAGAAAAGCGCCTCGC11.4492753623188406No Hit
GAATTACGCCGAAACCGATCAAGCTCCGCCGCAGCTGTTCCAGTTCGGCC11.4492753623188406No Hit
GTTTTATGAAACATAATTTTCCATTTGGGAAACAAAAAAACTTTTCTCAA11.4492753623188406No Hit
GAACCGACCGCGCCTTCAGCGGCGGGGGCCCCTCCGTGGGGGTTGATGAC11.4492753623188406No Hit
ATGGAATACAAGCGCCCCAGCGAAGTGCCGCGGTGAAAAAGCCTGTCTCT11.4492753623188406No Hit
TGCCGTGATAGTCCGGCGCAATGTCATGGGCCCCGGCGTAGTTGAGGGTC11.4492753623188406No Hit
ACAAAAAGATATATTATCCTCTTTTATTATTTCATTTCGTTACTCAATTA11.4492753623188406No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)