FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n02_Tr4_C12I3a_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n02_Tr4_C12I3a_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8
Sequences flagged as poor quality0
Sequence length251
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGATGTTCTGCTGACACCCGCTGGCGGAATGACCGAACTGCTGTTTGGC112.5No Hit
TAGTAAAAAAGGAACAAGAACTAGATCCAGATTTTTCTCCGCAGCGGGGC112.5No Hit
GCGTGGTGTCCAGAACGCCGGAGGATCCGTCAATGCCCATTTGCACGCCG112.5No Hit
ATATTTATCTACTCAAGCAGTTGCCCATATGATTGCTTTAATAACGTCTT112.5No Hit
GATCTGGACCGACGGAACACGGTCGGACGAGATCGCCGAGGTGTCTACGA112.5No Hit
TGCCTCAGTCACCTTGACGGGCCGGAGCATCGAGGTCGAACGCAGTGTGC112.5No Hit
TCCATATCATAAAAAGAAGGCCCCATGGTTTTCACAGGAGCCTTTGAAAA112.5No Hit
TCATTACTTAGAGCGCATTTCACAATTAACAGGTATTCCACTTTCTATTT112.5No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)