FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n02_Tr4_C12I2c_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n02_Tr4_C12I2c_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65
Sequences flagged as poor quality0
Sequence length251
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCTAGATCCCGAATGTTTTTCATCGTTTCTTCCTCAATGTCCTCTACC11.5384615384615385No Hit
GGGCTGGTCTTCTGGGATCACCCTACCCGACCCCCGCTCCCGCTGCCCGC11.5384615384615385No Hit
ATTTTAAAATGGGGCAAATGAGGGAACAGTGCACTTAGAACGTTTATGTT11.5384615384615385No Hit
CTCAACCACAGGCTTTGACAATGCAGAGCGGATGAGGCTCGAATTGTCTG11.5384615384615385No Hit
TCCAAGTGCTTCACTGCCCAAGCAGCTTCACACATCAAGCGCATTTCCCT11.5384615384615385No Hit
ATGTAGGCTGTTGATCTTCTTTCAAGGTAGAATCCCTCCTTCAATATCTC11.5384615384615385No Hit
CCTAGGGGGTTCCTTGGCCGGAATTTAGGCTCAAATGACCCTTCGGAGAG11.5384615384615385No Hit
ACTTATAGAAGAATCAGACAAACTGGACGTCAAAAATGCGATTGATGTTC11.5384615384615385No Hit
CCTCCATCCCTTTAGTGTGGAAGATCCTGCCGATATGTTTGCGCCTTTGT11.5384615384615385No Hit
GTTATTTATCGAGCTGGATATAAAATAAGTAGGCATGTGACTTATTCTTC11.5384615384615385No Hit
GAACTAAAGCGCGATTTCTATTTTGAAAATAGGGGCATGTCAGAAGAAGA11.5384615384615385No Hit
AGATATTTTAGGCGGGCAGCCAAATGCCATCTTGAAAGACGATGCGGCTT11.5384615384615385No Hit
GTATAGAGAGATACAGTTAGAAGTTGTGGTCATGTGGCTCGCTTTCTAGT11.5384615384615385No Hit
CACCTGATCTTAGTGCGATGATTGATTTCTTTGTGGAAAAAGGAATTGTG11.5384615384615385No Hit
CATTTAAAGTGGTATGAAGAATCGGGCCATGTTATCACGCTTGATAAAGA11.5384615384615385No Hit
CATCCACCATTGCCGTCAGCACATCGGCTGGCACGTCATCGGCGATGAAA11.5384615384615385No Hit
TATCTGCCTCATCCCCTACCATCTCTCCAAACTGTGTGAAGGCATCCTGC11.5384615384615385No Hit
TAATCAGTCTAATAGATTGAACAGTTGAAAAAGCGCTTTCTTTTGAAATA11.5384615384615385No Hit
GCTAAATAGATAAACTTTGTAGCTAATAAAGACGACAACACCATAATCAA11.5384615384615385No Hit
GACTAACGGCGATATGGACTTGACTAGGCATGTGGGGTTCGTATCGGTAT11.5384615384615385No Hit
GTCGACAATCTCGGCAAGTCTTTCGGCGGCGTCAAAGCCGTGGACGGCAT11.5384615384615385No Hit
TATTTAGACAGCTTAAATAATGGGCTCACATCTGCCATTGAGCGTTTCAA11.5384615384615385No Hit
TCTTAACACCTCAAGAAATAAAAGAACTCATACAACTTATGAAACACTTA11.5384615384615385No Hit
GGCGATGGTTGACCTGATGGGACAGTACGAGCAACACGGCCTGCAGCTCG11.5384615384615385No Hit
CCACAACCGTCGCAGCAAAGGGCATGGCGACAAGGTCCCGCTGACTATCG11.5384615384615385No Hit
TTCCTGTACACACGCTCACCATTTTATTAGTCCCATTCAGCCCCACATTA11.5384615384615385No Hit
GCCATTAATGCATCCATCATGCGCATAAAAATTTCATCGATCCGTCCACC11.5384615384615385No Hit
GGAGTGAGGAGATTTGTCAAACCTTTGATATTCCGATTCGTATTTGCCCT11.5384615384615385No Hit
ATACTGAACCGTTCATACGGATGAGCGCAGAAAGCTCTGCTTTTGTGCAG11.5384615384615385No Hit
ATATATAACGGAGCGACTCAAGTGATCAAGGAACTAAAACATCAATTTAT11.5384615384615385No Hit
GCGCTCAAGAGCCTCGACAAGACAGGCCGGCTGCTCTACGTGGGCCCGCT11.5384615384615385No Hit
TGTATCGCCGAAATTTCGTAATGCAGCCCATTTGCTTCTGCGGCTGGCTG11.5384615384615385No Hit
TTTTTAAATTTTCAGCGGGTGTGTTTCGTCTCATCTCCATTGTTTTTGCT11.5384615384615385No Hit
ATATTGTACTGCGCCTCCGTCAAGGCGTCAATGTTTTTTCATGTTAATCC11.5384615384615385No Hit
GTGTTGAGATTGATAACGGCTCATGGCCGGTCCCGCCTATTTTCTCCTTT11.5384615384615385No Hit
AATTAAAAGAGATTGAAAAATGGGAGAAAGACCAGCAAAAGGTATGGTTT11.5384615384615385No Hit
GTGTCTGACTTCTATCACTTTTATAGCCGTGAATGGATATAGAAAAGGGA11.5384615384615385No Hit
TTGTAGTACTGATCACGTATAAAACGAGGAGTAGAATGCCCCAAAACCAA11.5384615384615385No Hit
TTCCTCCCACGACTGCCTCGCTTTCGGTCCCGGGGCCGGCTTCTACCCTG11.5384615384615385No Hit
TGCCAACAGCGACAGGACGAAATTAACTCACCGCGCAGGTTCGAGGCTGT11.5384615384615385No Hit
AACATATTCTTGTCGCTTCTGTCCACGTCGTTTATTTTGTACGGTCTTTA11.5384615384615385No Hit
GTTAAATAAAGCATTCCTTTCATATCACTTCACATCCTTTAAGACATATT11.5384615384615385No Hit
GAGGATGGAATTCCGCATCTTGATATGGTGAGAATAGAAAGATAGGCGTG11.5384615384615385No Hit
TTATTGTTTCTTTTCCATCGCGGACAATCTTTTCATCGCCTTTAATTCGC11.5384615384615385No Hit
TGTTGGTGTGTTCATTCGTCGCATGGTTGAGGAGGAAGTGCTGCCAAGTC11.5384615384615385No Hit
GTCCTACGCCCCTCAAAGACCTAACCGTCCGGAGGAACGCGCCATCTTGG11.5384615384615385No Hit
GCTCATCTTCTTCTTGGACGAGGCAGTCGCCTTGGTGATTGACTCGGGAA11.5384615384615385No Hit
TGTCCAAATTCATGTGTGAACACACCTGCTGCGCCATCTTCAGGCTCAAT11.5384615384615385No Hit
CTTCTAGGCTTGGCTGTTTATCTCGGTTGTACCAATAGCTGCTATCCCAT11.5384615384615385No Hit
CGTCAGGTGTTCCACTATGACTCTTATATTTTAAATTGTTTGATGAGCTC11.5384615384615385No Hit
CTGCTGGTTGCCGCGACGGCGTGGTGCGCGCATGCCCTCGGTCGCCGGGG11.5384615384615385No Hit
TCATACACTTTATACACTGGCCAAGCTGCTTTTTAGACAAAATGAGAAGA11.5384615384615385No Hit
ATGCAGATTGTATCACGATGGATGGGCGGCAATGGCTCAACCAATCACCA11.5384615384615385No Hit
GATATACCGAAGTGGGGAACTTATTTACGCGAGAGATGGCGTGGACGTTT11.5384615384615385No Hit
CTCTACATCTGGCAGTGCCATTTGCTGAATCACAATGATAGCTGTAAAAA11.5384615384615385No Hit
GTGTACGACACCCTAGAACCGTCAGGGCGGGCGACAACCATCAAAGTCCA11.5384615384615385No Hit
AGTAACAGGGCTTATCCCCATAGATCTAATTTCACGCATCGAGAGAATTC11.5384615384615385No Hit
ATGTCCGGCTCTCTTTGCTGATCTCAGTCAGACTGACGGAGCGCGCATAA11.5384615384615385No Hit
ATATCCTGAACATCAATATCAATCCCAATGGCTCCCGATCCGTCCTTCAG11.5384615384615385No Hit
CTCTTATAGCTTCTGACAGCGCCCGCTCCAAATAAGGTCATCACATTAAT11.5384615384615385No Hit
CTTTTGGTCTGGCCGGCGCTGAGGATGCGTTCGACGGCGACTCGTCCGCT11.5384615384615385No Hit
TAGCTGTTTCTGTCGATTGGCGCTGGTGCGTCTAGTTTGTTTTTGATTCC11.5384615384615385No Hit
CAGGCATTGTGAATCAATTCTCTAGATTAGAATTAGCCAGGGTTTCATCT11.5384615384615385No Hit
GTACCAATTGGTGCGCATGTACCGCATCCAGTTTTCCGCCACCCCCAATC11.5384615384615385No Hit
AAGAATTACAGGCAAGAGAAACATTGGCTGAACTTAAAACCATGCTGACA11.5384615384615385No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)