FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n02_Tr4_C12I1b_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n02_Tr4_C12I1b_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42
Sequences flagged as poor quality0
Sequence length251
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCTGGCTGCACGCTGGTCTTCATTCCGGCGGTGGGCGAGCTTGTGATC12.380952380952381No Hit
AGATAAGTTGCCCTCTTTTTTTAAAAGGAGGAAGGTGGACTCATGATTAG12.380952380952381No Hit
GCAGTAACAATGCAAGTATGAGATATGGGGGGTGTATAAATGAATCAATA12.380952380952381No Hit
GCGCTCCCCCGCTGTGGGGTGCGGAGTCCGTGTAGCCGAGGGAGGAAACA12.380952380952381No Hit
ACGCTGCACTGCTCGTCCTTCTCGAAATGTCTGGTGGCGGGGTTTCGCAT12.380952380952381No Hit
GTTGATAATGGCATGCTCACCAATCACTGCATCTGCTTGCACAACAGCCC12.380952380952381No Hit
CATCACGTGGTGATGCTGAAAAGCATGTAAAAAAAAACGACAGTGGTCAG12.380952380952381No Hit
GCTAATTACAATGCGATCCGCAACTGGCTATATCTTGATATGGCGGTATT12.380952380952381No Hit
TCCGTATATCCATCGTCAAATGTGATAAGAACATACTTTTCACTTGGTGT12.380952380952381No Hit
TTGCAATATCCTTCAGCTGTGGTACGGAAGCCATGACTTTATCGATTCCA12.380952380952381No Hit
GTGGTACACCTGTTGGTGCGCGGCTACCTGCAACCGGCGAGCGCGCGGCA12.380952380952381No Hit
GGTTAACGTGATCGGCTTCAATTCGCTTCCTCCTCTTGCATGTCATTGGC12.380952380952381No Hit
CATGAGTACGTGGTCGTAGACCCGAAACCAGGTGATCTACCCATGTCCAG12.380952380952381No Hit
CGCTGTGATTGGTTTTAACAGCGTGAAAATGATTTTCCAGTAGATTTGCC12.380952380952381No Hit
GGGCGGAAACGGCCGCGGAGTTCACCAGCACCCGCCAGTCGTGCAGACGA12.380952380952381No Hit
CACTGTGACAAGAAGCCCATAAGTGCCAATTCGGTTGGCAATAAAGTTCG12.380952380952381No Hit
GTCAGAAGCACCCGAATGCAACCATCTATCTCACTGGACAAGCGCTTGCC12.380952380952381No Hit
CCTCGGCGTCGCAGCCAATATGGCTTACGGCCTGCAGCATGTACTTACCA12.380952380952381No Hit
CGCGTAGTGTACTATGGACTGGCTTGAACTACTTATACCGCATGGCGTCA12.380952380952381No Hit
GCATGGCGGCCTCGTCGAGCTCGAAGTCGGCCGTGAGCGGCACGCCGGCG12.380952380952381No Hit
GATATGGCCCCTGGGCTGTTGCTCACGGATATCGCTTTCGCCCAGCCGTT12.380952380952381No Hit
CAGCAAGCGAAAGAAGAAGCTGCTGCACCACAAGGTTTCAATACACTGAA12.380952380952381No Hit
AAATAATAGAGAGCTTGCGACTTTTCATCAATGGAGAGTAGTTCTTTTAG12.380952380952381No Hit
AGATAATAGTATGGGAGCAATAGCAAAGAGCTACGAGTCAATACGAAGTA12.380952380952381No Hit
GGATTATCTTGGACCATGGGCTTCCTTTGTGACAGGATGGACGTATTGGT12.380952380952381No Hit
GTATTATAATTCCTTGTTTTTTGTCAACTGTAAATCAGGCCGAAGCGTGA12.380952380952381No Hit
GTGCATACGAGCTTTGCAGATCTTCGTCCGAAAATCAAGCAAGACCCAGC12.380952380952381No Hit
GATAAACATCGATGCAAATAGCATAGCTAAAAATAGCCCAACATGTATTT12.380952380952381No Hit
CTCAAGGACAGGTATGTCCTGTTTATTAGTGTGACCACTCCCACAAACAG12.380952380952381No Hit
GGGTTGAAGTGAAACCAAGGAGGCATTGCCTCTTTGGTTTTTTAAAGCCC12.380952380952381No Hit
GATTACGGGTATTGCACATGTCAATGATGTCACACAAATCAAAGTCCCAG12.380952380952381No Hit
GAAGATAACCATATCAACGTCTTCTGGACGTTTAACTGGAGAAGGTACTC12.380952380952381No Hit
TGTCGCGACATTGCATGATGAACCAAATGTTAAAAGTGATGCGCCTGATA12.380952380952381No Hit
TTACAACGCATCAGGGGTAACAAAGCATTTATCGCGAAAATTCCCTGTTT12.380952380952381No Hit
CATTAACAGTGTCGATTCCAGTGGCGATTACGCATCAGAAGGTCAAAGAA12.380952380952381No Hit
AGTTAGAGGTTAAGTTAAGCGAAACGGATAGAAAATTAAGAAAAACAGAA12.380952380952381No Hit
TGTGGCGACCCCGACTGGGCATTTATTCGTATGGCACACTTGTGCGCGAA12.380952380952381No Hit
TCGCCAAGCAGGGCGGCACCATCCTAGGCACGTCCCGCACCAACCCGTTC12.380952380952381No Hit
TTTAGGTGGTGAATGGACACCAATTGAGTGGCCAGAGAATGTTCACGTTT12.380952380952381No Hit
GGGCACGGCCTGGCGTCGAGCGGTGGCGTTGGGCGTTAGGGAAAGCGGAT12.380952380952381No Hit
TGATCATGCCATAGTCTATGATTTACGTTACGCCGTTGGTCTATAGTATG12.380952380952381No Hit
CCATTTTTATTTGTAGCAGAAGGATTTTTAGCAAGTGCTTTATTGCATGA12.380952380952381No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)