FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n02_Tr4_C11I4c_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n02_Tr4_C11I4c_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57
Sequences flagged as poor quality0
Sequence length251
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCGAATTGAAGCCCCAGTAAACGGCGGCCGTAACTATAACGGTCCTAA11.7543859649122806No Hit
CTACCAGCCACCGCTGAGAAAGAACCGCGGAAAGGTCAGAAACTGCTCGA11.7543859649122806No Hit
GAAGTGTATTGGGGCTGCTATCGCGAGACGGCGGGGGAGATGCGTCTGGT11.7543859649122806No Hit
GACTAGGATCGCACTAACGACAATCAGAAGTAACCCGCAATTGCAACAAT11.7543859649122806No Hit
CACTACAAAACGCCGCGCACGGTAACCGTTCGCGGCGTTCTGTTGTGCAG11.7543859649122806No Hit
GACCATGACCTTGCGGCCGTCCAGCGTGGCGAGGCCGCCGATGATCGCGG11.7543859649122806No Hit
ATCCGTCATATTTTGATCCATGCTATTATTCGCTTTTTCATCCTCTGGTA11.7543859649122806No Hit
TCTCCAACCAGAGCATCATCTACGCGCTGCGGCCTGAGGCGCGCTCACGC11.7543859649122806No Hit
TATATATGGTTAAGCATATATGTTTGGGTGGAAAGAGGGAAGGCGGAATG11.7543859649122806No Hit
TCTTATAAGAAACCTCGTGAATTAAACTGGATGGTTGGTGTAGGTATTTT11.7543859649122806No Hit
CCTACAGTTGGAATGCATTCCACCTGTAGGAGCTGCCGAAGGCTGCGATC11.7543859649122806No Hit
TATGCAATGTCCGCCGAGCGGATATCTTGCAGGCTGCACGAAGGCGTTCT11.7543859649122806No Hit
ATGTTAATGTTGATCCGGTGGCCCTGATAACGCTGATTCAAACTATCGGC11.7543859649122806No Hit
GGTTTCGAGATTCGAGTCCATCGGTTTGATAATGAGGTGTTGAACCTCAG11.7543859649122806No Hit
GGTCAAGCGCTTGCTGATGCCGGCGCTGTACAACCTAAGTCGCGACGGCC11.7543859649122806No Hit
AAAGAGCATATTCGGACTTGGAATCGTCATTTTAGCCGTATGATCACCCG11.7543859649122806No Hit
TCGTTTTTCCTGGCGCCACCCGCTGTCACCGACCAGGGAAATATTGACGC11.7543859649122806No Hit
ATGGTGCCCAGGCCTTGGCATTGTGGCGCTCGGAGCCGTTCGACATTGTG11.7543859649122806No Hit
GTAAAAAAAGGAACGCCCGTGTCGGATTTGATTTTTGCCCGGCTGCCAAA11.7543859649122806No Hit
TGTACGTACTATTTGATTTGAGAGCGTATCGCAAGCGGGCTTAAGGCGCT11.7543859649122806No Hit
CATGACCGGGCTCGTAGTCTTTGTCTTTGCCTGGTGGCGCCACTGCTCCC11.7543859649122806No Hit
GAAAAGGATTGGGAGAAGATTCATGAGATTTCTCGTGATCGATACCAAAA11.7543859649122806No Hit
CTCTTATTTACCCTCATTTTCCTTTATTTAATGCTTTTTTTTCGCAAAAG11.7543859649122806No Hit
GGCTCAGCCTGGCTCCCACGGTGGCCATTGATGAGCGGGGCTCCAGGAAC11.7543859649122806No Hit
GGATGATGACGTGGTCCGGGACCAGGTTCACGAACACCGTGGGCTTGATG11.7543859649122806No Hit
GTCCACTGCGGCCAGCTCTGACTTTTATCCGCCCGGTTCTCAAAGCGACG11.7543859649122806No Hit
TAGGTGGACACTTCATCGTTGACGATATAGGTCAACGCACCTCGGTAGGA11.7543859649122806No Hit
CTCTATATGAGTGCGTGGATTGAAATAGGTAGTTCACTTACTCACGCCCT11.7543859649122806No Hit
GCCTACTGTTTCACGGATTTTCTGCATTTCGATTTCAATGAAGTACTCCA11.7543859649122806No Hit
CAGGTGTTGCCTGCAGCCCGGCCTGTGCATGATCCTGCGGCACAGCGCCC11.7543859649122806No Hit
CGTTAATTTCATTAAATGTGTAAGAAGAAAAGCCCCCATTAGCGAAATCC11.7543859649122806No Hit
ACGAAACCCCGGCTTTTTTTCGCCCCTGCAAAATGGTTATATCGACCTAA11.7543859649122806No Hit
TGACTCGCCAGGCGCGTCATACTCGAGTCGCTGCTGACACCGCTGCAGGG11.7543859649122806No Hit
AAAAACATGTGTAAATTGTCTTTTACGGACGGGGACGGATTCCACCAGAC11.7543859649122806No Hit
TGCTTGAATAAGAAGCCCGGCTCTAGAGCCGGGCTTTTTTATGCCAGATT11.7543859649122806No Hit
CTTTACGACCATTTATCTGATTCTTGCCATCGTGCTTGCCTACTTATTCA11.7543859649122806No Hit
GTGGTCGATCAGGTCGCCGATCTGGATGCGGCGGTGTACACCATCATTCA11.7543859649122806No Hit
CAATTAGTCCGGCTGACCACCCGGATATCCGTAATCCCTACCCGCTCGGC11.7543859649122806No Hit
CATACCAGGTACGTGTTTTGTATTTTTTAACTGATGACCCAACCCATAGG11.7543859649122806No Hit
CCACTCATTGATACGTTTGAAGTGATTAAGGAGCAGCTGAGCCGAATGGC11.7543859649122806No Hit
ATGAATTTCCTTCTTTAAGAATAAACTTCTCAGAATAAATATACTCATCG11.7543859649122806No Hit
ACCCTCACCTGTATCGTACAGTCTCCCTTTTATCCAAGCAGATGCTGCTA11.7543859649122806No Hit
GTTGATGATGGCCGAACGGGCCGGTGGCATGGAGTTCAAACGGGTGCTGT11.7543859649122806No Hit
AGTGTTCGGGCTTGTGTGGGGGTTTTTCTCTCTGCTGTTCGGCTTCGTCT11.7543859649122806No Hit
TTCGGGATGCTGGTGCAGATCGTGGCGGATGGAGACAAGTTCGGGAACAT11.7543859649122806No Hit
CCTGAGTTCCTCGTGGGCTCGCTCGCGCCGGACGGCTGCCGTGGTTCCCT11.7543859649122806No Hit
CAGCCAGGCATTATTCGGCTGACACTCCGCTATCGCGAGCAGGCTCGCTC11.7543859649122806No Hit
AGAGGACTCGCCGGGTGACGAGAAATCTTGTTCCCTACGCAGGCGCTAAC11.7543859649122806No Hit
ATCATAAGTGACAATCCATTTCCTGTTTCTTAATGTAGTCTGTATTTTGT11.7543859649122806No Hit
TAGCCCACCCCCCTTCCCCCGCTCCCTTTTCCTCCCTCCCCCTACCTCTC11.7543859649122806No Hit
CACTCCGGGTGTGGGGCTCTCCTGTAATGGAGACAGTGACAAAACGTTTC11.7543859649122806No Hit
TAATAAAAAAGAATGAAAAAAGTAGTAGGTGATGAAAATGAATTGGAGAA11.7543859649122806No Hit
CATAACGCAGTACCAAACAAAACCGGCCATGCCGATCGCGGTACGGGTCG11.7543859649122806No Hit
AATAACTGGTTTCTTCGGTTGTATGCGACCCCTCGGTGCCGGTGGTGCCG11.7543859649122806No Hit
ATGTCGAGCTGAAGGGGTGTCGGATCAGGTAGCCCCAGCTTCCGCCAGAG11.7543859649122806No Hit
GTTCAGTGCCGCGCAGCTTGCCGTAGTAGCGCTTGTCCTCGCCAAGAATG11.7543859649122806No Hit
TGCCAAGGGCGGCATGCCGGACGATCTACCGCTGATCGCCGCCGAGCGAA11.7543859649122806No Hit

[FAIL]Adapter Content

Adapter graph