FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n02_Tr4_C10I3c_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n02_Tr4_C10I3c_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81
Sequences flagged as poor quality0
Sequence length251
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCTTTGTCTTTCTCCCCTTATTTTCTCACTTCCCTACCCCTTTCCTCC11.2345679012345678No Hit
CTCTCAGATTATCTCCTCATCAGGCTAATCACGGTTATTTGACCCGGTTT11.2345679012345678No Hit
GTGTACGAGTGGACTGTCGCACACACCGCCGGCTCCACGTACGCGGGACT11.2345679012345678No Hit
GAAGTACAGCAAGGCGATCTTCGCGCCGGCCTTGCTGCCGAAATGCTGGT11.2345679012345678No Hit
CCTGCAAGCAGATCAAGGTGAGAACCGGTATCACGAGAACCGATATCCAT11.2345679012345678No Hit
AATTACGGAACATGCTCTGCAGTCACTCCCAAATAGTTATGTCTTGAATA11.2345679012345678No Hit
TCTATACACTGTCGCTTTGACAGCGTGAAGGATGTTGGGAAGATCAGTGA11.2345679012345678No Hit
GTTGTACTTCCTGGTGAACATCTTGCAACACATCCCGTCCCCGTGGCACC11.2345679012345678No Hit
ACAGGGTCAACGCCGAGCGATCCATGCCAATCAGTGGCACCTGAAGAAAA11.2345679012345678No Hit
CGATCAGTGTAAGCGCTGCCGGGATGGCGGCGCTTTTTTTTGCGCGCTGG11.2345679012345678No Hit
GCTGGCCACCTGCGCGTACGTCACGGCAACGCCGATGAACCCGCGGCCGT11.2345679012345678No Hit
ACATCATCGAAACCTCCGGCGTCACCTGCGTGATGGTCACCCACGACCAG11.2345679012345678No Hit
GTGGAGAATGCCGAACGCGGAGCGGCGGGACGCGCTTCGACCCGCTTCGG11.2345679012345678No Hit
CCATGGAACATGTCGATCACATCCTGATCGTCGACGACGACCGCGAGATC11.2345679012345678No Hit
ATGGCATGTATGGCAACGAGCCGGCGAAGTGGGACAGTGAACTCAAAGGC11.2345679012345678No Hit
GCACTCCTGCGCTACTTCAACCCGGTGGGCGCGCACCCGTCCGGCCGGAT11.2345679012345678No Hit
GCATGACCTTGTCGCCCACCACCAGCCGGGCACTGCCCTGTCTCTTATAC11.2345679012345678No Hit
GAGAAAATGGACGCCAAGGTTTTCGTTCAGTCTGCAGATGCCAACGAGCA11.2345679012345678No Hit
CCTCTATACCCGCTACAATGCCGACGATTACCGCGTCGATGTGGGCGCTG11.2345679012345678No Hit
AAAGCGCGCATTGGGGGGCGATGAGCGTATGGTGCACGAGCGCACCCGAC11.2345679012345678No Hit
CCGGATACGAGGCAGGCAGGCCCATCGATCCCCGGGCGGCCCGCCGGATT11.2345679012345678No Hit
AGGTTCACCAGGACATCGGCCACTTCAAGCGTGACAGCCAGTGGCACACC11.2345679012345678No Hit
CGCATGGGCTGCGCGTTGGCCGTCTTCTTTCACTGTCTCTTATACACATC11.2345679012345678No Hit
GTACGGCACCGTCGCGCGGCGCGCCCATTGAAAGGCCAGATGCGGCCAGA11.2345679012345678No Hit
ACTCAGGTGAGGACATCAACGCGTCAGCCCGAACGTCACAAATGATAATT11.2345679012345678No Hit
GGGCAGTACCCGCGGCCCATGGAATCAGGGTGACGACTGGGAGTTCGTTG11.2345679012345678No Hit
TGCTCAAGCTGCTGGGCCTGGAGAAACCGGCGGAAATGACCGGCACTTCG11.2345679012345678No Hit
AATAAGAACTGTTCGGTCGAGCTCATCGCCGATCTCGCGCGTCCAGATTT11.2345679012345678No Hit
GGCTCAGCGTCACCCCGGACAAGAAACTGGTGGTGGAGAGCAGTGGCAAC11.2345679012345678No Hit
AGATCGGCCAGTGGACTGCCGGACCTCGGCGTGCAGATGGCCGATCTGCT11.2345679012345678No Hit
CTCTGGCACTGGCGTTGTCGCCGAAATTCGACCTGCGCAAAAGCTGGTTC11.2345679012345678No Hit
GTTCTTCCGCCGCTTGGGCGAAGAGGCGGCCGAACAGGCCGTGGCCCGGC11.2345679012345678No Hit
CCATCGAATACCCCACCAGCGATCCAGATCTTACTCCGCCAGCGCCTTGT11.2345679012345678No Hit
CTTTGATGTTCATGTGCCGTTCGGTCATCAGGTATAGCGGGATCCAGGCG11.2345679012345678No Hit
GTTATCAGGTGGGTACGCGCAACATCGTCGACGTGCTCGACGCCCAGCGT11.2345679012345678No Hit
GGCGCATTTTCTGCGCGGGTTCAACGAAATATACCCATGGTGATGATGGC11.2345679012345678No Hit
GCTATATGGTTCCCCTGTTTCCGATAGCGTAACCCTTCCTTATATCAATA11.2345679012345678No Hit
ACTTTTTACGGCGCTGACATCAGCCAGTTGCGGGCTCTGGCCAAGGCAGC11.2345679012345678No Hit
GTAAACAGAAGAATTCGGCGATACATGGCGGCCTCCTGGACCGCTGTCTC11.2345679012345678No Hit
ACCTTGGGGATCGTGCAAACACCGGCGATGCCGCGGCGTTTGCGGCATTT11.2345679012345678No Hit
GGAGTTCTTTATCGCCTCAGCGGACGCGGACTTTACTTCGCTGATTCAGC11.2345679012345678No Hit
GTTCAAGGCCGTGGCCGAACAGACCAACCTGCTGGCGCTCAACGCCGCCA11.2345679012345678No Hit
TCGTACGCAACTGCGCCGACATCGGCACCGGCACCGGCCTGCTTGCTTTC11.2345679012345678No Hit
ACCCACCGGTCTGAATCGACGAATACATCTGCGCCACCTTGTCCTCGCGG11.2345679012345678No Hit
ATGTTTCACCACACGGTGTGTTGCCCGCGGTCCTGCCCTGTTGGCCGCAT11.2345679012345678No Hit
ACGTCTGCCTGGACGAGTTCACCGACCACGGACATTGTGGCGTGCTCGAC11.2345679012345678No Hit
GCCTTCGCGCAGCATGATTAGGCAGGGAACGAGCATTCGCTAACCACTGT11.2345679012345678No Hit
CTGGAAATCAGCGCCGCAGCAGCCGACTCACCCCGGTCCACCGTCCTGGC11.2345679012345678No Hit
ACTATCATGCGCGTACGCAGTTGTTCAGCGCCGGCGACATGCTGCTTCCC11.2345679012345678No Hit
TGTCAAGCGAAAGTCGATCGTAGATACAAAGCAGGGCGCCGGCTGCGGGG11.2345679012345678No Hit
GTGGTGCGCGAGGCGATTTCCAAGCTGCAGGCGTCCGGGCTGGTCGAGAC11.2345679012345678No Hit
GCTCCGCCACCGTCGGATCCGGCGTCCGCGAATCCGCCGGTGGGGCGGGG11.2345679012345678No Hit
CTATACTTCCGTGTCGGCGGACTTTAAACAGAAGACTATCCAGAAAATGA11.2345679012345678No Hit
TTCTACGACCCTGCCCAGGCGTGCTGGCGCATCCGCCATCCCGTTGACCC11.2345679012345678No Hit
TTTCATATATTAAGTGAGCACCCAGTGGGTTTAGACGATCGACTCCGTCC11.2345679012345678No Hit
TAGACACCAACTCCCGTCACAACCCAAAGCATACCCTGTCAATGGGGCGT11.2345679012345678No Hit
GCTACAACCTCCGCAAGCACGCCGCAAGCGGCGAACTTCTCGCCTTGGCA11.2345679012345678No Hit
CATCACGTAGTTCCGACTAAGCAAGAAACGCTGGCACTGATTGATTCCGC11.2345679012345678No Hit
ACTTAAAGCTCCCGCGAATGCGGACCGCTGCCCAACAGAACTGACTTGCT11.2345679012345678No Hit
CCTGGGGCTGAAGCCGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTG11.2345679012345678No Hit
AGTACAAGGACGCGATCAAGTACTTCATCTCTTTCGAGCCGGGCGAGTCG11.2345679012345678No Hit
GTACTGCCCTTTGCTCGCAGGCCGCGCCTGCAACTCGTCCACGATCATGC11.2345679012345678No Hit
GGTCCCCAGAAAGGATACCGGCAACGGGCGCCGCAGCCGGGGTCGGCGGG11.2345679012345678No Hit
CTTAAAACACGCATAAGCTTATAAAAAATAATCTTGCTCAATTGCAAGAC11.2345679012345678No Hit
CCGTCATCCCATTCCACATACGCCACACGCCTATTCTTCTTTCATTCTGT11.2345679012345678No Hit
GCACCTACCAGTCGCGGCGCGGCGCATTCACTCCCCGGCCGCAAGTACCG11.2345679012345678No Hit
GGTCCGTGCCGTCATGACCGAACTCAACGACGAAAAGATCGACATCGTCG11.2345679012345678No Hit
CTTTGTACCCCAGCCGTCCGTCATAAGGCCCGAGGCACTGCAGCGCTATG11.2345679012345678No Hit
AGTTTGAGGAGTGGAAGAAGAAGCGAGCACGCAATGGCCCCACCGCTCTG11.2345679012345678No Hit
GTGTTTTTGGCTGGCTCTGGCAACCGTTGGTGTGGCGTCTGGTGTGCACC11.2345679012345678No Hit
GCCAACATCCATGGCGGCGTTACCCTGCTTGCCAACTCGGATGTCTGTCT11.2345679012345678No Hit
GGTTATAATGCCCGGTCTTTTGTGTCAGGCAACGTCTGAATCGTTACCGC11.2345679012345678No Hit
GGTGCATCTCTTCTCTGTTGAGGGGGTATTACCGATCCTGCAACGTACAG11.2345679012345678No Hit
GTACCACCCGGGCGGCCTCGATTTCGATGGCACCAACGTCTGGGTGCCGG11.2345679012345678No Hit
GTTCATCGTCTGTCTGGTTGTCTTTACAGCATTCCTTTTCCTTTTCACAA11.2345679012345678No Hit
GTCGTCAAGGAAATGCTCTACATAGCCGTATCCGTCACCAATGCATGCTC11.2345679012345678No Hit
CATCAGCACTTCCTCGCGGTTCAGCGCGCCGACGTCCACCTCGGTTTGCT11.2345679012345678No Hit
CGGCAGCACCGGCAACGCCGCATTGACCGCCAATGCGCTGCCGCTGCGGG11.2345679012345678No Hit
ATATCTGGGGCATTCCCGGCGTCGGCCCGGGCCTGTTGCAGCGCTTGCAG11.2345679012345678No Hit
AGAAGCGTGTTTTGTGGGCTCGATATACCCGTCGCGCTGGGTATACGACG11.2345679012345678No Hit
CTACGGTATTTGCGCTCTCCCCTCCTGCTCGCTATCCTGCGTCCCCACAC11.2345679012345678No Hit

[FAIL]Adapter Content

Adapter graph