FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n02_Tr4_C10I1b_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n02_Tr4_C10I1b_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58
Sequences flagged as poor quality0
Sequence length251
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCCAGGTGACTGCGCAAGCCGTGGCTGATGGCTTTGGTGCAGATGAAA11.7241379310344827No Hit
GCGGGAGGGCATGCGGTGGTCGGACATGTCCTCGGCGGAAACCTTGGGCA11.7241379310344827No Hit
CCACATCACCCAACTAGCCACACCCAAGGCGCCGCGTCGCTGCACAGCCT11.7241379310344827No Hit
GGGTGTGGATCGAGATACTCGCTTCACCCCGACCAATGTGGGAGCTTGCC11.7241379310344827No Hit
GGCGGGGTGGGGGGTGGTCGTGGGTGAAGTAGAAGTAGTACTCGGTCGGT11.7241379310344827No Hit
GGTGGAGTAGATCTCCTGGGTGCCGGGAACGTCCTCGGTGGTGATGGTGA11.7241379310344827No Hit
GGCCTTTTGCTTCGTTCGGTAATACACCTCCGAACCTAGGCGACGTTAGA11.7241379310344827No Hit
ATTCCATAGCCATGGCTACATTCCCAGTCTCGATGCCTGGGTCGTGCTCG11.7241379310344827No Hit
TCTTAAGGCCGAGAGCTGATGACGAGTTACCTTTCAGGTGACGAAGTGGT11.7241379310344827No Hit
GTCTCGAGAACGCCTACCCGGCCTTCATGCGCCTGTTCTCAGGGGCGACC11.7241379310344827No Hit
CTACAGCCACTACGGCTGCAAGGAAAACCTGCTGCGCGACGTGCTGCAGG11.7241379310344827No Hit
ATGGCGGTTGTTCGGAAGCGTTCCCTCCGGCCCCCGGCCCCACCCGCACC11.7241379310344827No Hit
TCTGGTCGACCGGTTGTTCACTGTCTCCTCAGCACTTACATTCCCCCCCC11.7241379310344827No Hit
CAACACCATCATCTTCCTACCCCCCCCTCCCCTGTCTACTTTCACCACAC11.7241379310344827No Hit
GGCTTGAATGTGGTCGGCCTGATGAACACCCAGTTCGCGGTGCAGGTCAA11.7241379310344827No Hit
AAATCAATAAACGACATACAATGAATAAGTGTGACGTCTTGATGACATTT11.7241379310344827No Hit
GTGTTAAGGTCTTCCTTGATCAGATTGTTCAGCTTCCAATGCCTCCGGCG11.7241379310344827No Hit
TTCCGAAACGGTGAGAGGACCAGACGCAATCTTATTCCGAATGTCATCGC11.7241379310344827No Hit
ACGTAAAGATCGCCAAACGGACCCGGGCGGATCGCCGCCTCGGTCAGGGT11.7241379310344827No Hit
GTGTTACAGTTGGAACACTCAACGTCGAGATGACAGTGGAGCCGATAATG11.7241379310344827No Hit
TTGCCCAGGTGCTGGACTTGAAGGACTACCAGCAGGCGCCGTACCTGGTG11.7241379310344827No Hit
TAACTTTCTTTCCTTCTTCTATAGCAACTCTCGTCCGCCCTTCTATCCTC11.7241379310344827No Hit
GATTTGAGCAACTCGACCGGAACCTTTCGGCCGCTCGTCGTTAACCAACC11.7241379310344827No Hit
ATCCTGGGCAGAGGCGGCAGCCACAACCTCATGGGCCGCGCCTTCATAGC11.7241379310344827No Hit
GTTCATGGGCTTTGCGCGGGCCGGAGATCATGTTGACGTAGGTGGTGATC11.7241379310344827No Hit
TTACTTCACGCACACTCTCTCGCCCGGTCTTATTTCGATTTCCCTTGGTC11.7241379310344827No Hit
GCAAGACTGGGCCTGATTACGCCGCCCTGTGCCGTTTGGACACCGCGAAG11.7241379310344827No Hit
ACAAAAGGTCATCAGTACCGCTGCCTCTCCAGATAGCCAGATGCCTTGTG11.7241379310344827No Hit
TACTATCCTGACCCTCCAAGAGGAGAGACAGGGCTTCTCCTCCTCAAAAG11.7241379310344827No Hit
TTACTGCCCCCCTACAGTGGCCCCTTTTTGCATTCGTCGACCAACCACCG11.7241379310344827No Hit
TACTCCAGCCCTCAGCCGAGGGCCAGAGGCTTGAGGCTTTTACAGCGGCG11.7241379310344827No Hit
CTACAGCTTTTTGTTTATTAACTTCCCTGTCCTTGCGCTAACCTTTGGGA11.7241379310344827No Hit
GTTTCCGCGCCTGCCAGAAGTTCAGGCCCGAGGGGCAGCTGGACGAATCC11.7241379310344827No Hit
TCAAATACAAAATATACAAGTATTTGGCTCCTATCTCTTTTAGTTTTTTG11.7241379310344827No Hit
ATTTGAAGGCGGCCTCGCTCGGTTTAGCCCACAGAGCGAATCCATCGACC11.7241379310344827No Hit
ATTGAGCGCGCCACCGGCCAGGGCCGTCAGCGAAACGAACAGCAGGAACG11.7241379310344827No Hit
GAGAAAATCACGTCCTGCTTGCGCGAACGGGCCATATTTTCTATGCCCAT11.7241379310344827No Hit
AACTTTCCAACGAGTGGGCTAGCCGGCAAAACGGCTCATCGGCGGCACAC11.7241379310344827No Hit
TCGATGGACACCTCCAAGGGCGGCAATGCGATTCCGATCATCTTCGGTAT11.7241379310344827No Hit
GATGTCCCCTTTCCTTCACCGCCCCCCCGCCCCCTTCCCCCTCCTTCGCC11.7241379310344827No Hit
AGTATAGGATTTCCTTGTCTCGAGTCCAAAAGCCGCCCATTTATATACTC11.7241379310344827No Hit
TTGTTCTGGCGTTCTTGTTTAGGTGTCGCTAGCTCGCGCACCTACTGTCT11.7241379310344827No Hit
GGTTTCCGCTTCTTCGAAACCCAGACCTTCTATCAGAAGGGCACCGAACT11.7241379310344827No Hit
CTAAGGCTCCGTTGACCGCCGGGACAGAATGGATGCGGCACACTGATTGT11.7241379310344827No Hit
GGTTGCGTGAGCTCCTCTCCTCAGCTCCCGGGAGCATCGACGCCGGCACC11.7241379310344827No Hit
CTGGTAGCTGCTTGCAAGGTGCGGTTTGGGGGGCGCGTCTACGCCATTAC11.7241379310344827No Hit
GTACAAATACCTGATCTTCGGCATCGCACTGGTCCTGATCATGATCTTCC11.7241379310344827No Hit
TGATTAAACAGCACCTGAGCCTGCTCGTAGAGCCTGGCGCGTTCGGCCGG11.7241379310344827No Hit
CACTGACCGAGGAATGCGCATTCCCCACCTCTCGGATGATCCACGCGCAA11.7241379310344827No Hit
GAAGTTAGGTGATCTTGCCCTGATATGCGCACAAAGAACTTCGATAAACA11.7241379310344827No Hit
AACCATGGTCATGAAACTCCACCGCAGAAACTTCCTGGGACTCGCATGCG11.7241379310344827No Hit
GGTCGGACCTATGAACGACGCGCCGATCTTCTGGAAAGCCGCTCGCTTTC11.7241379310344827No Hit
CTTTACTACATGGCGCGAATGCTGACTGCGGGCGGGGAGCCGCTGTTCGT11.7241379310344827No Hit
GGTCGGCCGCGTGGCTGCTGACGAAGGTGTAGTCGGAATGCCATCAACGG11.7241379310344827No Hit
GGAGACCCGTATGATGTGAGCGGGGGCCGGCACCATCCGAGGGGGACCGG11.7241379310344827No Hit
GACGTGGTGGGTTGGGATGGTGGGTTCCACCGCCGCCAGGGAGGGACTGT11.7241379310344827No Hit
CACGAACGGGATGACGTTCACCGTCACCGATGATGCCGGTTCGGTGGTGC11.7241379310344827No Hit
GCCGCCGACGCCCCGGCTGCCATTCGGACGCTGCCGACGATAGTAACGGT11.7241379310344827No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)