FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n01_Tr4_NCI2c_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n01_Tr4_NCI2c_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44
Sequences flagged as poor quality0
Sequence length251
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCAGCTCACCGCCATCAATCCGGCCACGACGAACAAGCTGCTCGGCCTG12.272727272727273No Hit
GAAGAAGAACTTCACCAGCTCATAGCCGTTCTGCTGGGCGAGCACGTCGG12.272727272727273No Hit
GAGCGAGTCACAGCCCTGGCCGCTCTGACCGACAAGCCCATCGTCCTGCA12.272727272727273No Hit
TCCCCGAATTTGCGCTGGTGATGCCGCGCATGCTGCACTGATCGGGAATC12.272727272727273No Hit
GGATGCGGTCGACGTAGTGGATGTCCTGCGCGCCCTCGACCTTGGGCACC12.272727272727273No Hit
ATGCTGTCGAGCGGTTCAGCCTCGGCACCCAGCTCGTCTCGGACGACCTG12.272727272727273No Hit
GTCGTGGCCTGCGGCGCTGCAGTTTTGGGGGCGAGGGTGGTGGCAGACAT12.272727272727273No Hit
AGCCAACCCAGCAGCCGCCCGCTTGCTGGGTGCGCAAGATCCCGTGCAGC12.272727272727273No Hit
AAATACCCCACATACCAGGATTGCAGCCGCTGCTTCCACACCGCACGTTT12.272727272727273No Hit
GATCGTGGCCGAGCACTCGGCCCGCGTGTACCGACTGGCCTACCGCCTGA12.272727272727273No Hit
CCTGAGTCGGTTTGGGGTACGGGCGGCTCTAGATCTCGCTAGATGCTTTT12.272727272727273No Hit
CATCAAGAAGCCAATCACGCCCTCGTAGACGAACCGTGCGTAGGGATAGA12.272727272727273No Hit
GTAGAAGATGGCGGTGTATTGCTCGGTCATCCTGGCCGTGTAGTTCTGCA12.272727272727273No Hit
AAACACAACCGATGCCGTTGCGATCGGGTTGGGCATCCTGGCGCTCGGCG12.272727272727273No Hit
CCCGTGTGGTGCCAGCCGAAACGGCGCGCGTTGGCGGTGGCCTGCGGGTC12.272727272727273No Hit
CCGCCATCGTGCTCAAACGGCGGCTCTAGACCGCCAGTACAGCCTGACAG12.272727272727273No Hit
CTCTTGGCGCCCACCGAGGTGCCGTCGACGAGGATGTCGCCCTTCTGGAA12.272727272727273No Hit
TATCAGCGGAGGCCACGTCGCATTGGATGACGGCGGGTGGTTGGTTGGCC12.272727272727273No Hit
GGTTGGCTGCGGCCGCCGTCGCTCCAGGTTCCGGTAGCAGCGGCACCATC12.272727272727273No Hit
CCTTATGGGCTTCTCCACGTCGTCCCTTTTTGCGCCCAGCGAGCACTTGA12.272727272727273No Hit
ATCTTCAATGACTACATCGATGCGGCGCTGGCCGGCCTGTTCATCTTCGT12.272727272727273No Hit
GCTGATGCCCATGTCGATCTCCTGGTACGTGAAGCCCTCGGAGTCCGTCG12.272727272727273No Hit
GGCTTCAGCGGCAAGGGCTGCTCTGGCGCCGGGCTGGCGAGATCGGTCTG12.272727272727273No Hit
GTGCATGACCGAGGCGACGTGGGTGTCGTCGAACTCCAGCGAGTGGATGA12.272727272727273No Hit
ACATCAAGGCGTTTTTCGCGCTGGCGCTGGTCGGCTGCGTGGGCGCCTGG12.272727272727273No Hit
GTCGATGCCATCTTCGAACCGCACGAATGCTCCACCGCCCGCTGGCTGCG12.272727272727273No Hit
GCCGTCGACTTGGCGGTAGATTTGCTTGCCGTGGTCGACTTGGTCTTGGC12.272727272727273No Hit
ACTAGCAACAGCGTGTGCCTACAGTGAGGTTTCGATCTCCACGCCCTCTC12.272727272727273No Hit
ATGCAGTGGCGCATGCTCGGTCGGCGCGGTCGCCACGCGAGCTGGACGAT12.272727272727273No Hit
GAATTAAAAAAAGCCCAGTCTTCATCGACTGGGCTTTTTGCTTTGCGCCT12.272727272727273No Hit
GCCTGATGGGATCGGCCAACCGCGACGCGACGGTCTTCGCCGACGCCGAC12.272727272727273No Hit
GGAAAACAAGATCACGATCAAGGCAAGCTCCGGCCTGTCGGAAGCCGAGA12.272727272727273No Hit
CAAATTGACTAACATCAGTAGCCTGCTGCACATTGGCCACCGAGCCCCTG12.272727272727273No Hit
GGATGAACTGGGCCTGACCTATCCGCAATACCTGGTGATGCTGGCGTGCT12.272727272727273No Hit
CTTCAGACCGCCCATCGGGAAGTATTTGATGGAGCTGCCGCCAAAATCTT12.272727272727273No Hit
CTGTGGTTCCTGGTCGCGATCGCCGTCGGCACGGTGATCAGCCTGTCTAT12.272727272727273No Hit
ATCCGGACCGACGTGCCCGACGCCGGGGCGTATGCCCTGCTGAACTCGCT12.272727272727273No Hit
GTATTGCGCTGTTGACGTGCAGGCCCGCCAACGTCTCGCGCGTCATCCGC12.272727272727273No Hit
ATACGCTTCAGACAGCGCTGATGCGCCAGCAGCGGTCATTGAATGAGTGC12.272727272727273No Hit
CCTGCGGGCCGTCGTCGCCGCGGCCGGAGACGTGGCGCTCGTCCTGTTCA12.272727272727273No Hit
GGCCAGGTCCGCCGGCGGACGCGGACGGTCGAAGACGTCCTTGAGCGGGA12.272727272727273No Hit
GCCGTACCTAACGTACGAGGATTGCCGGAGGGCCGGTTCGGCCATGCCTA12.272727272727273No Hit
GGCACGTTGGTTATTCTGGACGTGTAAGCTGGGCGTCGTGTGCCTCCGCC12.272727272727273No Hit
GCTGCATAGCCACCGCTGGCCCGCCCGAGCAGTGCGCCCAGCCATGGGGC12.272727272727273No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)