FastQCFastQC Report
Mon 22 Aug 2022
000000000-KG266_l01_n01_Tr4_NCI1c_T1_rc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n01_Tr4_NCI1c_T1_rc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157
Sequences flagged as poor quality0
Sequence length251
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACGAAACAACATACCATTGGCTAAATCGAGCATCATGCGATCATGCTC10.6369426751592357No Hit
GTCCAACGGGGTGGGGGCCGCCACGAAGCAGCGCTTCACGCTGACGGTGA10.6369426751592357No Hit
CATGAATAAAGCGGTCTCCTCCGATGTACATTCCTACATGTGAAATTCCT10.6369426751592357No Hit
TCGGTCGTGAGCGGTTCGCACATCGCCTTGCCCGAGCGCACGTCGAACTT10.6369426751592357No Hit
GGCGTGCTGTGGCATCCGCGTCGGTCTGGTTGAGCGTCTGGATGGCGTTG10.6369426751592357No Hit
GGCCAATACGCAGGCGCTGTCAGTGCAGGCTGACAGCAGTCGATGCGCCC10.6369426751592357No Hit
CGGTAACGTGTTGGAATCTGTGACATTAAAGATGTATCCGGAAGTCGATA10.6369426751592357No Hit
GCCTCGCCGGCGGATCGCTGAACTACGGACCCGGCAGCCACACCGACTGG10.6369426751592357No Hit
GTCATCTCATTGATAAAACCTATGATGAAATGCACTGTTATCAAAAGAAC10.6369426751592357No Hit
CAGGCATACCTTACAGATGGGGCGGCAATACACCAGCTGGATTTGATTGC10.6369426751592357No Hit
GTATTGAATATCCACATCGAAACCCGGCTGTTGGAGCGCAGTGGCACGGC10.6369426751592357No Hit
ACGTGGCACCGATCCTGCTGATCGCGCTGCCGATGACGCTCATCATCATC10.6369426751592357No Hit
GTGCTGTACCACGCCGCCGTCGGCATGCAGTTCCTGCACGAGCTGGCCGG10.6369426751592357No Hit
GTTATGGGATGAAGTTGAGAAGGCTGCGAAATGAGGGCTTTCTAAACAGA10.6369426751592357No Hit
GGGTATCTGTATGTCCTGACCGATGAGACGGACGGGCAACTGTTGAAAGT10.6369426751592357No Hit
GTACGGGAGCGGGGTGCGCCTCCGGCGTGCCGCCAACGCGGCCGGCAGCG10.6369426751592357No Hit
CAAATGAAGGAAAGCCGTCAAGGTCCTGTCCTTGTTCGTTAGCTAATAAT10.6369426751592357No Hit
TTCCGCGACCGCTTCCGGAGAATATGCCGGGACGCGGACCACCTTCAGCC10.6369426751592357No Hit
GTCTGAACCAAATTGAACAAATTTTTGCGTATCAATTCCTAGTAAATGTA10.6369426751592357No Hit
GAAAAAGCCATCACTCACTATCACAAGCTGGGCATCGAGCCGATGAGCAA10.6369426751592357No Hit
CTATTTAACAATACAGCAAAAGTTTATTACTATTATACAGATGAAAATGC10.6369426751592357No Hit
GTGACGCCAAGCTCTCTTTCCAGTTCCGCAATCGCCTTCGACAGCGCCGG10.6369426751592357No Hit
ATTGTCGACCATCAATTGCAGCAGGGGTCCGACGAAGTGGCCGAGCGGAC10.6369426751592357No Hit
ATATCGAGGCGATCCGCCCGATGAAAGACGGCGTGATCGCCGACTTCACC10.6369426751592357No Hit
ACATTTAACAGCCTATGAAAATTTAGAAGCGTTAAGAAAAATTTTAGGTG10.6369426751592357No Hit
GTCATCGAGGGGGAGAGCCGCACCCCGGCGCAGGAGCTCGTGACGTCGAT10.6369426751592357No Hit
GCCCGATCTCGACGCCGTCTGGAGCCACTTCAAGGCCAAGCGCAAGTTCG10.6369426751592357No Hit
CCCTGCAAGCTGGAAGCAAGCTGTAGCAGCGGCAGATGAATCAAACGGGA10.6369426751592357No Hit
CCTTATGTCATGAGATGTACGTTATTGTATTGATGATTGATTGTTTATTC10.6369426751592357No Hit
GTAATGCGATAAACATGAACGAATAATCAAAAAATGTATACGGTCTTGTT10.6369426751592357No Hit
ACGATCCCCGAAAATACGCCGGTGGCATCGAACACGCCTTCGGCCTGCGC10.6369426751592357No Hit
TGCTGGTGCTGCTGCCCGCGCTCATGGTCATCCTGGGCCGCTGGGTCTTC10.6369426751592357No Hit
CACTAGCAACAGGCTTCGCAGTTGTTGCAGAATTCGGAGCACCAATTGTG10.6369426751592357No Hit
GGACCACTCCCGCACGCGCGGTGGAATGGGGGTCGTAACTCACCGCTCCA10.6369426751592357No Hit
CTACCAGACCGGCAAGGAAGTCGGCTTGGATCCACTGCTCATCCTGGGCT10.6369426751592357No Hit
CCATATGAGCTTGCGTTGACCCTGCGGCAGTTCCGCCAAGGGGATCGACA10.6369426751592357No Hit
TGCGTACCCCCAGCACCGCCAGCGAGGCCAACTGCATGGTCGGCTGGAAT10.6369426751592357No Hit
AGATTGCTCAAGCTGTGGCTGACTTTGAGGTCGCTAAGACCGCCATTGGC10.6369426751592357No Hit
CCATAGGGCTTAGTTGCATAAACTACGATCGAAGAAATACCTTCCCTTTT10.6369426751592357No Hit
CGTCCGGCCCTTCTTGACGATCAGCTGGCGGAACGTCTTGACGGTCTTCT10.6369426751592357No Hit
GTGTGAAGCCGCCGTTAGTCAATCTCGGCGTAATGGCCCTGTTTAACGCC10.6369426751592357No Hit
AGCCAGCTGTGGTTGTCGCACATCGTTCGGTCGTTCGAGCGTACTCAAGA10.6369426751592357No Hit
GGTCGACGCTGATCGGCTCGATCACCGCCGCGGGGCTCTCCGCACTGATG10.6369426751592357No Hit
GGCCACATGCACGGCCGTCACCTTACCGGCAATGGCGGCCTGCACTTCGG10.6369426751592357No Hit
GTATTGCGACCATGCGCCCCGGCGCTGCAGTTCCTCCTTGAGCCGCATGG10.6369426751592357No Hit
GGCTATTCCTGCCACAGTGCGTCGCAAGCGCCACAGCCATCCCTGTCTCT10.6369426751592357No Hit
GTCACGGCCCGCTTGATCGAGACCGACACCGTGACCGAGCGCTCGCCGCC10.6369426751592357No Hit
TGAACCATGTTGGCGAAGTCTGCGCGCAGGCCCTGTACCAGGGGCAAGCG10.6369426751592357No Hit
CTATCCGGCGGCCCACCGGCCATCACCCCTCATCTCGGGTCCTCACACAC10.6369426751592357No Hit
CTGTACATGCACTGCCTATCGCGCAACGCGGCCATGATGCTGTCTCTTAT10.6369426751592357No Hit
GACGTCTGACCGACTTCTGCGTCGAGCGCTACGGGGCCGAGGTCGCGCCG10.6369426751592357No Hit
GATCGACGCTGTCCATGATGCGTTGGCTGCGCTTTCGCGCACTAGAGTGA10.6369426751592357No Hit
CCTTAAGAGAGGTGGCGAAAAAAAGGCCCGACCCTCGAGAACAAGGGCCG10.6369426751592357No Hit
GGATCATACTTGCCATTTGTTAATTCAGCTAACTCTAGCGCATAGGCTGT10.6369426751592357No Hit
TGCAGGGGGTGACCGGGGGTTCGTAGTTGGGGCCGGAGTGGCCGCCCACC10.6369426751592357No Hit
AGGTTATGTTGTCAGGCTTGTCGCTGTTTACCAAACTCTTAAGTTGGCCA10.6369426751592357No Hit
TTTCTATGCTATTTCATGTCTTACCGTGGCTGGGCAATGTCAACTAGGCA10.6369426751592357No Hit
ACCGCTTCCAGCCGGGTGTACGACCCCGGTTACTACATGCTGCCCAATTA10.6369426751592357No Hit
GCGCTACACGGTCAGTTGCCCGGGACTCGTACAATCGGTCCATCATGCAA10.6369426751592357No Hit
GCCACACACATCTCAGGCCGTCAGAGCTATTACACCTTAAGTAAAAAGAT10.6369426751592357No Hit
GCCTCCACTGCCGTGGGTGCATCTTCATGCGCATAGCCGGTTTGCAGCTG10.6369426751592357No Hit
CCAATCACGATGGTCGTTGCGATGCGCCGCTGCTGGAAGGCGACGCGCTG10.6369426751592357No Hit
CCTTGCAATGCAGCGAGCTGTTGATCGTCAGCGGCGAACGCGTCTGGCTG10.6369426751592357No Hit
GTTAATACCTTTGCAAAGAAGGGCGGCATCAAATCTGGTCGTGGCGGCCA10.6369426751592357No Hit
CATCCAGGAGGCCCTGGCCGGCGGGCTGTTCGAGCCCGAGCGCACCCCCG10.6369426751592357No Hit
AAGCCGATGTCGCCATGTACGAAGACAAGCGGGTCCAGCACGAAGGCGGA10.6369426751592357No Hit
ATGCTGACCAGCGAGAACCCGAGGACGCTAACCGTGTACGAGCGGGCCGT10.6369426751592357No Hit
CAAATGGAATGCAATTGAATGGAATGGAATTGAATGGAATGTGAACGAAT10.6369426751592357No Hit
TATTCCCCCTCTGTACGCTCTTGCGTTGCCTCTTCCAGCAGATGTTTCAT10.6369426751592357No Hit
TTCGAGGCCTATGAGGACGATCTCGTCTCCCCGCCTGAGGAGCCGGACTT10.6369426751592357No Hit
CAGTGGACCAGAGCTTATGACGACAGCGGCAATCTTGTGGAAGCTGTCGA10.6369426751592357No Hit
ACTACGGGCTGAACGGCGCATTCCCCTCCGTGCCGGCCACCGAGCTCACG10.6369426751592357No Hit
TGTCACAGATGGATTTTAAGTGAATGATGTTAACAGATATGTACACCAAT10.6369426751592357No Hit
TGGCAGCAAAACCTCTATTACCAGCGGCGTGCGCGCCGACTTCTACACCG10.6369426751592357No Hit
GTACGGTATTTCTGGGGCACAACCTCAAGAAGTATTTGAAGAAACTTTGC10.6369426751592357No Hit
CATATCCTGCAAGCGTGCACGCGCCACGTGGGTGTAGATCTGGGTTGTGG10.6369426751592357No Hit
GTTCGCCAGCTGTACGGCGAGCCGACCATCACTGAACGACATCGTCACCG10.6369426751592357No Hit
GACCTGGGTCGCGGCCGTCCCGGCTGGCACATCGAGTGCGCGGCCATCGC10.6369426751592357No Hit
GCAAGCACCGCGTGGGCCTCGCGGTCGTCGACGTCGGCACCTGTCTCTTA10.6369426751592357No Hit
GAACGATGTGCGGTGAGAGCGAACAGGTCATCGGTGCAAAGTAGAGCTGT10.6369426751592357No Hit
GAGAGGTGGCCGGCGAGACCCGTGTACCACGTGTGGGCTGTCTCTTATAC10.6369426751592357No Hit
GCTTCGATCTGCTTGAGCTTGACGAATGCATCGGGATTGGTCGCTAGGGC10.6369426751592357No Hit
GTCCAGCGCCGCGACGGGGATCGCCGGCTGGGCCAGGCTTGAATCGATGA10.6369426751592357No Hit
CCACACACCTCTTGCATTCTCGGTAGCTGAAATGTGAACCAGTGGCCGCT10.6369426751592357No Hit
ACAGAAGCAGCACTCTCATTATGTTCAACGTTCATTGTGGATGTTTATAA10.6369426751592357No Hit
GGAAAGCTGATTGCATGGAAGTCTCTCGTATCCGGGCCTTGCGCGGCCCA10.6369426751592357No Hit
TCAGAGAGCGAACCCGCGACGAGGATCAGTGAGCCCAAGGTAATCAGGTA10.6369426751592357No Hit
AGTTGAAATAACGAACATATTCACCGACCAGATTATCACTATATGGCAGA10.6369426751592357No Hit
CGCCAGGAGGCCGACAAGGTTGGCTACCCGGTGCTCATCAAGGCGGTCGC10.6369426751592357No Hit
GGTGTTAGCATTAAGCCCGCATATAAAATGCCTGTAAACGAACGCTCTTC10.6369426751592357No Hit
ACGGGCTTCTTCTTGGCCGCCATGATGCCCTTGAACGACGGGTAGCGGGC10.6369426751592357No Hit
GTGCATTGCCGCTCTGCGTGAGCGCATTGATGCGCGCCAGCAGGTAGTTG10.6369426751592357No Hit
CAACAACGCCACCGCCATGAGCGGCAAGACCATCTCGTATGCCGTCTTCT10.6369426751592357Illumina PCR Primer Index 7 (95% over 24bp)
GTATTGATATGGCACTGATATTTAGAGATTATAGCTGCGAACCTCTGTCA10.6369426751592357No Hit
CTGGTGACCGGCAAGGTCGAGGCGACGTCAGGACGGGTCAAGAAGGGCAA10.6369426751592357No Hit
CCGAAGGAAGTCATCCGCTGGTACTACGGCGACCAGCAGCGCCTGGCCGA10.6369426751592357No Hit
CGGCCATGCACAGCACCACCATGGTCGGGTAGACCATCCAGCCGGCGCTG10.6369426751592357No Hit
GTACAAACGAACACCATCACAAATGCCTTTCGATATTCATTTGGTATCAG10.6369426751592357No Hit
CATTTGCACCCGAATAGCCCAAGGCGATGGCGGATGCGATCGCGACGTTC10.6369426751592357No Hit
TTCGTCGGCCCGGCCTGAACTGGTCGGCGCGCTGTCACTGGACAGGCTGG10.6369426751592357No Hit
CTGTACGTCCTGGTGGCCGCGGCCGTCTGGCTGCTGCCCGAGACGTCGCC10.6369426751592357No Hit
GAATTGTCGAGCATCCAGTTGCACGCGAAGTCGCGCACAGCCGCTCCATG10.6369426751592357No Hit
GTGATGGTGCCGTCGGCAACGGCGCGCGCCGCCGCCTCGAACTGCTGCAC10.6369426751592357No Hit
CTGCGATTCTTCAGCCTTTAGGGGGCGGGCCGATTATTAGCTGCCTTGAT10.6369426751592357No Hit
CCGATGGCTTGTGCCAGAACTGTCCCCAGAGTTGTCCTTTGACACCCCTT10.6369426751592357No Hit
TTTCATAACATTCCTGCGGGATCGGTTCTGTGGCGCCTGGCGCCCTTGTG10.6369426751592357No Hit
GTCCCACAGAGGGCCGTGGCCGCCTGGGTAGAACACCGTGTCGAAGTCGG10.6369426751592357No Hit
GTCTTCGCGATCATCACGGTGATCGGCGGCGCCTTCGTCGGCGGACGGTA10.6369426751592357No Hit
GTGTGCATCAGCGCGACGACCTCGGGGTCGCCCGCGTCGACGACGTACCG10.6369426751592357No Hit
CGCCGTACCAGTCGTCGACGCGCGCGTACGGCAGCCAGAACACCGAGCCC10.6369426751592357No Hit
GACCCGCCCGCGCGAGCACGACGGCGGCCGACAGACCGTTGGGTCCGGAG10.6369426751592357No Hit
TGATTGGAGTGAACACCATGAAGGTCACAGCTGGAATCTCCTTTGCCATC10.6369426751592357No Hit
GGACTCACAGAATGTCTTATCAACTGTCATGGTATTCCACATAGCATTGC10.6369426751592357No Hit
ATATTGACGATCTTTTCATTTTCATGATCAGGCCGCATATATACTCTTCA10.6369426751592357No Hit
GTTGGGGTCGCCGGAGATGTGCGCGATGTGCCGGCGGCCGAGCATCGCGA10.6369426751592357No Hit
ACCATGTTGTTCGGCTCGAAGGCAAGCTGTTCGATTTCGCTGTGGAAGTC10.6369426751592357No Hit
ACTACAGTCAGGAGGCTCTGTGGAGGGTCTTGGTACAAGAGCTGAAACAT10.6369426751592357No Hit
GCTGGATTCATCCGACGCGGGGGTTGCTGCCGCCGGCTGCGTTCATTCCG10.6369426751592357No Hit
ACGAGGTGGAACCAGTTCTTGCCGAGATCGATCCCGATGACGCGAACAGC10.6369426751592357No Hit
TTCGCAAGGAGATCGCACCATGGCGGTAGATGCAGTCAATGCGGCAGCGG10.6369426751592357No Hit
ACCCGTCTGTATAATAAAGATGAGCAACCTTGACGAGCTGTCGTCTTTCT10.6369426751592357No Hit
ATTAAAGACAATGCCAATTACGGAGATATGGAAGAGAAAGTGAAGAGCAC10.6369426751592357No Hit
TTTCTGAGGTGTGGGCAGATAGAGGGGTGCTGAAAGCTGTCAATGTGCCT10.6369426751592357No Hit
TGCGATCACCGCGCCATGGAACAACGGCTGCACATCACCGCGAATGGGAA10.6369426751592357No Hit
ATCCCGTACCCGCTCGCCGCGAAGGCCCTGACCGTCCGCTACCTGGGTGG10.6369426751592357No Hit
TCATCCAGGAGCTCGGCTGGGACGAGGATGTCGACGAGGAGCTCCGTGTC10.6369426751592357No Hit
GTATCACCTTGGTGGCGTTGCGCGCCGGATGGTTGCCGACGATGAGGTCA10.6369426751592357No Hit
GCTCCACACGGCGGCCGTCATGGACGAAGTGAAGGCGATGGCCGCACACG10.6369426751592357No Hit
CACCATTACAGCCACAAACGATATGGTCGTTTGCCATTTGTTCAATCGTG10.6369426751592357No Hit
GCTTACGAGTGAAGCAAGTACTTGAACGGCTCAAGGAGATGCGGGGCTTG10.6369426751592357No Hit
GCTCACCCCGCGCGACGCCTCCCGTGGTTCTGTCTCTTATACACATCTCC10.6369426751592357No Hit
GTCGTCCTTCATCGCCCCTCCTCGGAACTCAGCCCTGCATGGGTACCTCG10.6369426751592357No Hit
GGGCGGCACCGTGCCGGGAACGGAAGAAGAACCCCACTCGGTGCGGGACC10.6369426751592357No Hit
ACGAAGTCCAGCGCCCTCGCGAAGCCGCTCTTGGCTGTCTCTTATACACA10.6369426751592357No Hit
CTCCCACACGACCCAGGCCTTGCCGCCGGTGATGTCGGCCAGGGGCAGGT10.6369426751592357No Hit
GATGGCGTTCTACGTGACGGACGAGGGAGAGCGGATCATCGAGATGGATG10.6369426751592357No Hit
GCTGTGACCTCCGGGCGTCGAGCGACCAGTGCCGGCCAGCGTCGGTGAAG10.6369426751592357No Hit
CCTATGAACCAGAAACAGAAGGACAAGAAATGAATGTTCAAATATCTATT10.6369426751592357No Hit
CCTGAAGGCGGTCAAGAAGTCTTACGCGAAGGCCGTCAAGCGAGCCCACG10.6369426751592357No Hit
GTTTCCGCTGGTGGCAAAGGAGGGCGGGGTATTGGCGCGGCGCGGTCATA10.6369426751592357No Hit
GTCCATTGCCCGTCGCCTGCAGGACCCGCTGGCCGAACTGGTGAAGATCG10.6369426751592357No Hit
CTCATATTCTAGCGAAAATAAAGGTAAATAGCTATTTTCCTATCACCTTC10.6369426751592357No Hit
GCCGTAGGAGACGTTGACCTCGAGTGGCGGCAGCCCTGCGAGCTCGACCA10.6369426751592357No Hit
CCCCGCCGGCTTCGACGAGCTCATGCCCAAAACCCTCGCCACCGCCACCG10.6369426751592357No Hit
CAGCTGGTCGACCGTGGCGTTGCCGACACGCACGGGCCCCGAGTCGCGGT10.6369426751592357No Hit
CCGGAACCTTCTTCTCCGCCTCGGGGCGTCATAACTCGGATGGAAACCAC10.6369426751592357No Hit
TGTTAGGCCAGAATCATCAAACATGACAAACACTGGGTCATACGGCGTTT10.6369426751592357No Hit
AACTCGGTCAGGTCCTCGGCCATGCGCTTGGTGAGGGTGGTGACGAGGGT10.6369426751592357No Hit
CAGTTGATGGCGCTGGTGCCGAACCAGAAATGGGTAATCGCCAACCTCAA10.6369426751592357No Hit
TGATCAGGTTGGTCAGGCCGAGGCGCGTGATCAGGGTGTCGCGATAGGCC10.6369426751592357No Hit
TGACGTGCGCGCGCTCGAAGCCGTCGAGCACGGCCTGGCGCAGGTGCGGC10.6369426751592357No Hit
AATACGCACCGGGCGAACCTGCTGGGTATCCGACGCTGTGCAAGGGGCGC10.6369426751592357No Hit
GATATATCCTGCGGCTGCCATGCCGCCTGCGGCGACTCGTTGGCCTGCGG10.6369426751592357No Hit
GGCCCGAGTTCGACTGGGAGCGCGGCTACGCACGCTTCTTCGAGGAATAC10.6369426751592357No Hit
GGAGAATGATAAGGCAACTGCCTTAACACACTATCGCAAAGTCGTGTCAG10.6369426751592357No Hit
GGGCCTGCGCTTTCATGCCGATCATCGCCCGGAGTGTGCCTGGCAAGCGG10.6369426751592357No Hit
CTCCGGGGGCGCGAGCCGGCCGTCACGCTCACCGATCCGGTCGGCGAGCC10.6369426751592357No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)