FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n01_Tr4_NCI1b_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n01_Tr4_NCI1b_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42
Sequences flagged as poor quality0
Sequence length251
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAATGGTTAGCGCTCGCCCAGGCGCGTAAGGACTTTGCTCACAAAGCG12.380952380952381No Hit
GTAGAGAATACCAAGGCGCTTGAGAGAACTCGGGTGAAGGAACTAGGCAA12.380952380952381No Hit
TGCCGGCACCGTTGGCGCCGAGCCCACGAGACGTCTGCTAATCTCGTATG12.380952380952381No Hit
GTTCACCGAGTACCCGGCGTAGGGGCGACGTGACGACCGCCCGCCGCGTG12.380952380952381No Hit
AACGTATCCTGGTCATCGATGCGCCACAGCAACTGCAGATCGAACGCACC12.380952380952381No Hit
CTACCTCTCCTCCAGAAAGTTGCTCATGACACGCACCATCGCGCGGCTCG12.380952380952381No Hit
GGTTATAAAGCTCGTGGCGTAATTTCACGAGGCCGATGCCGGGGCGCGAA12.380952380952381No Hit
GTAAAACCACCACCGGCGACCACCACGGTATTACGCGCAGCGCTCGACGG12.380952380952381No Hit
AAGTTCTTCGACCTGTGCGAGATCTTCGGCGCCGACAACGTCGGGATGGT12.380952380952381No Hit
GAACGACTCGGCCAGGTGCTGCACCACGGCGACCGCGTCGGCCCAGCCGG12.380952380952381No Hit
CAGCTTACTGCGACCGCATGACGATGTGGAAACACACTTGCGATTGACCT12.380952380952381No Hit
GCATGGATCTGCGCACGCTCGGCCGGGTTGGTGGTGTAGTTGTCCCAGAA12.380952380952381No Hit
TCAGTGCATGGGTCGAAGGTGTCTAAGCAGAAAGCTTGCCATCTCGCCAA12.380952380952381No Hit
GCCCATGATGTGCTGACGGTTCTGGAAGCCCGTGTCCGTACTGACGATGT12.380952380952381No Hit
GCAGAAAACTCGCCCTTTAATGTAGCGTTTGAACTGAGAATTGACCCGTT12.380952380952381No Hit
TGAACCCTGTGATGGCGATTCCGTTTATCGTTACACCACTCGTGACAGGT12.380952380952381No Hit
TCGTACTGGTGAAGCCCTGCTCACCCAGGTGATCGATCAAACGTTGCGCG12.380952380952381No Hit
CAGCGCGTGGAGAGCTTCATCACCACTTGGGAGTGGGCCTGTCTCTTATA12.380952380952381No Hit
CCTTATGGGCCACCCCATGGGCCACGTATAATGCGGGCCTTCCACGTTTT12.380952380952381No Hit
TTGAAATCCCCCGCGTTTCGTAAAAAATTTCCGTTGTTGGTAACCGGCAG12.380952380952381No Hit
GATCAGACCACGCTGGCCTTTGCCGATCGGAGCGCACAGGTCGATCACAC12.380952380952381No Hit
CCACCCAGGCGAGCGCGGGGCGTGCGGCTGTCGATGGCAAACCACACAGC12.380952380952381No Hit
GGCGCCGTCACGGGCGGCCCTCGAGCGCCGGGTGGCTGAGGTGTCGCGGT12.380952380952381No Hit
CTTCCATGGGGTCGACTCGATTGCGCAGTGCCTGACGGGCAGCTTTGAAC12.380952380952381No Hit
CAGCTGGCTCATCTGCTGGCGAATCCAGCCGGCCCGGCGCGACACGTAAG12.380952380952381No Hit
GTCCTGCATTTCGCACTCGGTGGCGATCAGGGTTTCGACGCCCAGGCGCC12.380952380952381No Hit
TGATGAACCTTCTCCCGCTCTTTATCAAGCGTGATAACATGGCCCGATTC12.380952380952381No Hit
CTCCCACCTGGGTAGTGTCGCCTTCAGAATTCCTGGTGCGCCGGCAATAC12.380952380952381No Hit
GCGCTGATCCGTTGCTGCAGATCGGCATCAATATCGCTCGGGTGATGGAG12.380952380952381No Hit
GGCATTTATTCTGGTTATCGCAGACGTATACCTTTGCCGTCCCCCCGCTC12.380952380952381No Hit
CGTTACGCATCTGCGCATAACGGTCTTCCGGCACCAGCACCTGTTGCTGA12.380952380952381No Hit
GTTCGTGTCGAAATCGGACGCGCCCTAAAAAATGCCGAGCAAATCATCAC12.380952380952381No Hit
GTTGTGGATGCTCGGCTCCAGCGATTTTGGTGGGGCGCTGGCCGCGCGCC12.380952380952381No Hit
GCACACCGCTGGACTGCATGCCGCAGTGGTTGCAATGGGTCATGCAAGGG12.380952380952381No Hit
TCTCTCCACCACGTACCTCGACGCGATCCTGCCGCCGCGGCGCGGCTGAT12.380952380952381No Hit
GTCCCAAGCGCTAGCACAATCGCCAGTAACGATAACGTGTTCCCTAATCC12.380952380952381No Hit
GGCCTGGTGATCGTGCTGACGATCGTCCAGTTCCGTTACGTTGAACGCAA12.380952380952381No Hit
AGAAAGCGTAGATCCTGGTACGTTTGTGATCGTTAATGCTTTATGACCAA12.380952380952381No Hit
AAGAAAGACACAGTAGCGCTTCAAAATGAAGTGGTACAAAGCCCGCTTGA12.380952380952381No Hit
GGTCCTGCCAGTGGTCGACCCGGAAGAACTGTGCGACGCCTGTCTCTTAT12.380952380952381No Hit
CGACATGGAAAGTGCCGCTCTCGGCATTGAACAGCGGCGGCAGAGTCGCG12.380952380952381No Hit
TCGATGACCTGACCCAACTGCTCGAACGCGCCCTGTACACGCCCAACGTC12.380952380952381No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)