FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n01_Tr4_C8I4c_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n01_Tr4_C8I4c_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101
Sequences flagged as poor quality0
Sequence length251
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATGAAGTTTCCACGAAGATTTACCAGCGGCGTGCTGATGCTGTTATC10.9900990099009901No Hit
TCGCTTGATCCAACTACGGCGCCGCGCCTTCGATACGCACGCTTTGGCCC10.9900990099009901No Hit
TCTTTGGATTGAGGAGTGAGGTTGCGGAGGGATGCTAAGTTATGGAAAAC10.9900990099009901No Hit
TCTGTAAACAACTCCGGGTTTTCATAGATGCTTCCGATTCTAGTGTTTCT10.9900990099009901No Hit
GTGAGAGAGCTCGCTACCATTATTATTGCGAATCCAAACACCGTCAGCAA10.9900990099009901No Hit
AACCTGCACCGGCTCGCCACGGAGTCCCGGGTGTACAACTTTGTCCGTGC10.9900990099009901No Hit
GACCCGGATCGGCCTGTCCGCCACACAGAAACCGGTGGAGGCGGTGGCGC10.9900990099009901No Hit
GATCATTTCATGCGTTTCAAGTAGACTTTGCAGATCCTAAGTCCGCAAAG10.9900990099009901No Hit
TATATACATTACCTGTAGCTTTCGTTGTGCATGAGGCCCCCCCTTTCATT10.9900990099009901No Hit
GCTCCGCCCCCTACCGCCGAAGCCCTGGCCGTATGGCGCAGCCGCTGCAC10.9900990099009901No Hit
CGATACTGCTTATGGATATTGATTTCATTTCATAGATGAAGCGTAAAAAA10.9900990099009901No Hit
GCATTCCTACTGAATGCTGGTTTTTTTATTTCCATTATATAACTGGCTAA10.9900990099009901No Hit
GGTGGTCAACATCGCGTTTGGCGTGATGACCCGTGCGGCGCCGCAACTGA10.9900990099009901No Hit
CATTACAGAATGCAATTCTTTTCGTTTTGTTTCATCCTTCGCTAATACCC10.9900990099009901No Hit
CCCTGGCCTTCGTCTTTCAGACGCTGGCCAATCGCAAACCGGAGCTGGAC10.9900990099009901No Hit
GGCGTCTACTTTTGCGACTTCACCGACGGCCTCGCGCTGAGCGCGAAAGG10.9900990099009901No Hit
ATAAAGATCAGTCCCCTCTCCCTCCGGGAGAGGGCTAGGGTGAGGGGAAA10.9900990099009901No Hit
CATGCATCTGGCTCTTCTCGCGATCGGTTGGCGCCGGCAGCGCGACAACG10.9900990099009901No Hit
CTATTCATTTGCTCCGGCACCGGGTGTCGATCCGACGTTCGCGCTTGAAT10.9900990099009901No Hit
CGCGCAACATTTCGTCGATCTCATCGGGCTCGCCGATTAGCGCCATCTGG10.9900990099009901No Hit
GAGTATCAGGAGTCGTTGACGAAGGTGGCGGTGTTGTTGCGGACGTTGGA10.9900990099009901No Hit
CCCGGTGAGCACCAGAAAGATCGACTGCCAACCCGTGGTGTTGACCAGCA10.9900990099009901No Hit
GTACAAACAAGTGCAAGTACTGCTTTTGATTCTTTGTTTCAGATGAAAGG10.9900990099009901No Hit
GTCTGAATATGAAGAGCAAGAAATGCTGCCACGTGAAGAGCATGAAGAAA10.9900990099009901No Hit
ACCTGACTCATGCGCACCGGCATCGCATCCATCAGGTGGGTGCGGCCAGT10.9900990099009901No Hit
GCTTTATTTAGGCGGCATGTATTTAAAAAGCTTTGGGGCAAACGATGGCG10.9900990099009901No Hit
TTACAGACCAGCAGTGATCCAGCAGCTTCAAACGCTTGGGAAACAGCTTG10.9900990099009901No Hit
CCGTCAAATGACAGAGAAGCTTGAACCGTTCCGTTCATTCGAAGAGCATA10.9900990099009901No Hit
CTACCTAATCCTACGCGGGCTCATCCCTGGGCGATAAATCTTTGGTCCGA10.9900990099009901No Hit
GCCATGATATTGCAGATGAAATTGAACGCAGAATGAAGCAGGAACATGAT10.9900990099009901No Hit
GTCGAAAATACCCATGACGGTGCAAAACCGCCGCCCGCAAAGGCCCGTGC10.9900990099009901No Hit
TATCAGAAGATTCCTGCTCTCATGCCGGTAGCCATTGGTCGTGGCGGCCT10.9900990099009901No Hit
GCTTATATCATGTGTTTCATGGTGCTATTTCACCAATGGACGGAATTGGA10.9900990099009901No Hit
GTGTCATCCAGCGCTGCCAACAGAATCCGGCCCATCGACGTGCAATACGC10.9900990099009901No Hit
GTCAATATATTGTGTTGTCTGTTCATATTGACGGGAGGCTAAACTGAGGA10.9900990099009901No Hit
GTACCACGTCCAGCGGTGGGTTCAGGGCACCGGAATCCTCGTCCAGCATG10.9900990099009901No Hit
CAATTGCTGTCTCTAAAGACAATAAAGAGCTCATAAAAGAAATCAACGAT10.9900990099009901No Hit
GCCTTTATTCATCAATCGTTTCGGATGTGAGGGGATCACCTTGAACACAG10.9900990099009901No Hit
GATCACAGACGTAGGGCAACGATAACGAACTGGCTCCCCCGGTTTGTACT10.9900990099009901No Hit
CTCACAACAATGCCCTTTCCTTTAATAAAAACAAGCGTATTCGCTGTTCC10.9900990099009901No Hit
CCCAACAGCAGCTGCACCAAGGTACTCTTGCCGCCGCCGCTGGCGCCGAC10.9900990099009901No Hit
GGACAATTGGCCGGGGTACTTTTTCTCACTGCCGGTGAGGCCGACCAGTT10.9900990099009901No Hit
AAATCACATTTCATCTCAAGCAACTACGGCGGTGCCTGTACACAAGGGTT10.9900990099009901No Hit
ATACATGTCGGAGGCGCGCCCGGTGATGTAGAGGAAGCCGTTCTCATCGC10.9900990099009901No Hit
GTCCGCCAGCGACGGTGGAATGAAGGTGGCATTGTCCACCGTGAGCGACG10.9900990099009901No Hit
ATATTGCATTCGTCTGGCAGACCCCGCTTGCGGTGTCCGCCACCCAGATC10.9900990099009901No Hit
CAGGGGCCTCGTCGAGACACGCATCCAGTCAGTGACCATTCACAGTGACC10.9900990099009901No Hit
ACTATCCACAACGCGCCGCGCATGTGCTGAGCCTGATTCGCCAGAGTCGC10.9900990099009901No Hit
CATTTAAATCAATATCATGTACGACCTTCTTTTCCATATCATGATCTGCA10.9900990099009901No Hit
TCATAATACACTTTCGTATCTTCAACTTTTTTATATTCTTTATGCTGTTC10.9900990099009901No Hit
CTCATATAGGAACGCTTGCAGCATCTTTTCTTCACAAGTTCTTCTGAAAT10.9900990099009901No Hit
TTCAGTACTTCTTCACGACCAATGACATCTGGGCGGTCAACCGTAATTTG10.9900990099009901No Hit
ATTATGAGCTGGATACCATCAGCGGCAGCAATCAAAAAGAAGTATTCGCC10.9900990099009901No Hit
ATAAAAACCGGTCAGCGGGTCGGTCATCGCGGCGTCGCGCAGCTCGCGTT10.9900990099009901No Hit
GTCGAGGGACAGAGCCTGCGCCAGCGTTCGGGTGAGATGGCCACCCTGTT10.9900990099009901No Hit
CTATTATTCGTTAGAAGCACTAGAGAAACAAGGAATCGGAAATGTTTCTA10.9900990099009901No Hit
GGCCATGATAAGCAGCTTCGCGCGACAGGCCAAATCGGCGCTGACGAATG10.9900990099009901No Hit
CAGCAGAACACCCGTCTCCCGGCCGCACCGTCATGAGCGGCGAGGACCCC10.9900990099009901No Hit
TAACTATATTACTAAAAAGAATAAGCGAAACAATCCAGATCGCGTTGAAT10.9900990099009901No Hit
GACCGCTACCGCAATCGCATCATCCTGATGTGGACCCAGTTGGCCATGGG10.9900990099009901No Hit
GTGTTTATTGACACCATCATTGTTTGTTCTATCACTGGCATTACGATTGA10.9900990099009901No Hit
GCGGTGGACAGCAGGGCTGCCCGCACCGCATGGCGCAGCAGTGTCGATTC10.9900990099009901No Hit
CCTCTCGGCCGCCTTCCGCAATCTGGACGCTGATGCCAGGATCGACCTGA10.9900990099009901No Hit
ACTGGGGCTGGATTCCAGTGCCCTCGACGATCCGGATGCGCGTTTCCCGC10.9900990099009901No Hit
CATAGACACACTGCCTAGAAGCCAATGAATAATCTCTTTTAAATCATCAC10.9900990099009901No Hit
TTCTACGACACCATGCGCACGCTGGAACCCGACGAGAGGATATTCCGCAT10.9900990099009901No Hit
GAAAAATACGTAGGGTTCGGTGGTGATCTGCCCGGCCTGTTGCAGGGCGT10.9900990099009901No Hit
ACCCAGATCGCCAGCGAGCCGAGAAACAGTTCGGTGTTCTGCGCGAATAA10.9900990099009901No Hit
TTATGGAACGAGCCAAACAAGATCCCGCATTTGATCTTGAGCAGCAGCAA10.9900990099009901No Hit
CTTCGATCTTGCTCTCGCTTGCGTTGCTCATTTGCTCACCTCGTGGCGTG10.9900990099009901No Hit
GCAGTGGACGGGTCGGGGCTGGGAGTTGCCATTCAGGTGGACGGCGGCAT10.9900990099009901No Hit
AGGTTGCGCAGCAATTTAAACAAGAGGGTGACTATTTGAAAATGCATGCG10.9900990099009901No Hit
GGACAGTGAACTGGTCTACTGGCGCGAGCAGTTGCGCGACGTGCCGCTCG10.9900990099009901No Hit
CATCTGGGCGCTCGGTGTATCGCACAAGCGGGAAGGGATTATATCCTTGC10.9900990099009901No Hit
GTCTACAAGGACAAATGGGCAGGAGTGTCTTCGAAGAACGCCGCAGACGA10.9900990099009901No Hit
GTCAAGATATGGTGAACAGATGAATATTGACCATCAATCACCCATTTCTT10.9900990099009901No Hit
GACCTTTGCTGGCCACTTCGACTTCGACGGCGCTGTTGCCGTTTTCTTCC10.9900990099009901No Hit
GGAATGATGCCAAAGTCAGCGCTCACCACGGGATCATCCCCACCATCGCC10.9900990099009901No Hit
GCGCTATTCCTTACAAACGAAGCCGTATTAAAAGGAAATGCACCAATAGG10.9900990099009901No Hit
CCGCCAAGGTTATGTCGCACCGGCGCACAGTCCGGGCAAATACTGCCGAA10.9900990099009901No Hit
TCTTCCCAGCGGCGGCGTGATTCCACGTCCATGGGGGAAAGCTTCCACTG10.9900990099009901No Hit
CTTAAAGGAGATTTTGAGGAGAAAATTAAAGAGGCAGCGAAGCTCATTGC10.9900990099009901No Hit
ATCCATGACTGTGTCAAGAGACGGATCAGGCATATCACCCATTAACCTTG10.9900990099009901No Hit
CGGCATCAGCGGCCAGCAATTGGCCTTGAGCGGCGAGTACGCGCTGTCTC10.9900990099009901No Hit
GTAGTTTCCTGTCTTTTTCTGTGTTGCAGCAGCAAAGAATGTGAGTGCTT10.9900990099009901No Hit
TCAGTGCCCCGGCGTTCGCCGCCTCGCCCAAAGAGCTGAAAAGCGAACAG10.9900990099009901No Hit
TCATCGGGCAGATCCAGCGCGAGCAATTGGTCATAGCCGCCCTGCACCAC10.9900990099009901No Hit
ATAATACGGTCATCAAAGAGCAAGGGGCGAAATATATGAATGCAGCGATC10.9900990099009901No Hit
GGTACATGACCCGCAATAGACATGAGTTTTTCTTTTTTCTCTTTAAATGA10.9900990099009901No Hit
CGTATGTGCTGGGACGCGGCGGCGAGGAAGCCGAATACTGCCGCCAGCAC10.9900990099009901No Hit
CCTGGTTTGATATGACACCACCTTGCCACTTTTCGGACAAAAAAGAGGAA10.9900990099009901No Hit
TGCTCATCCTCATATTACAAAAGCCATCACGAAAGCAGCCGAGACGGGTG10.9900990099009901No Hit
GCTTCAAGGCCTGCTAAAACAAATAGAATATTTTCCTTTCGGCAGCTATA10.9900990099009901No Hit
GTTTGTATCCTTCGATCAAGACGGCATCTAAAGGAAGATGCTGACACATG10.9900990099009901No Hit
GCCGATCACCAGGGCGATGGTGGTCAGCACTTCAAAGTAGATCACCGATT10.9900990099009901No Hit
GGTCGGCTCGGCGCCCTGCGATGGATCAGCTAGCCCTTCTTCGTAACTGC10.9900990099009901No Hit
TGGATATGGCACGCTCTCGACGGCCTTTGTGCCGGATGAGGACCAGGGTT10.9900990099009901No Hit
GGATAGGCACGCCGAGGAACCACAGCAGTAATGAGCGCATTGTTTTCCTG10.9900990099009901No Hit
GAGCCAAACCTGCAAGCAAAAACATGAGTAGAGAAACAAGCAGGATCGGG10.9900990099009901No Hit
CGTAATAACTGCTGCGGTTGATTCCAAATACAACGCACAACTCGGATCTT10.9900990099009901No Hit
GGTCGAGTGTGGGAGCGAGCCTGCTCGCGAAAGCGGCGTGTCAGGCGCCA10.9900990099009901No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)