FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n01_Tr4_C8I1b_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n01_Tr4_C8I1b_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80
Sequences flagged as poor quality0
Sequence length251
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCATAGGCACGCGCTTGAACGGACCCTTGAAGGAAATCGTCTGGCCCTG11.25No Hit
TAACAGCCATGATTGTAGAGGCAGGGATTTCATTTGGTGCCACAAGCGTT11.25No Hit
GTTCAGAAAAGGGTTTGTTCATGCAATACCCAGTTCAAAAAATGCCTGAT11.25No Hit
CCTTCAGACCGACGGCTGCGTAGATGTGGCCGGCGGTAGCGCCCTCAACG11.25No Hit
AATTAGATATTTATGTTCAAAGCCACGCCAAACCCTCCGGGCATCGACCC11.25No Hit
GTTGCGAGGTGCCACATGAACGACCCCAGATTCCCCAGTGAAGAGCAGGG11.25No Hit
ACTTCACTGAGGACCAGATCGTCACCTCGCTTGAGGACCTGGGCGCAGCA11.25No Hit
GTATTTTCTCCGTGCGCAAAATCGCTGGTTTTCGATCCTCGATGAAGGTG11.25No Hit
CGCCACGCCAGCGAAGGTCTGCTGGAGGTCTTGCTGGAAGGCCGCAGCGT11.25No Hit
ATGAAAGGAAGCCAAACTTCAGCATCGTTTAAATCATCGATGGAGACACA11.25No Hit
GTCCTTTTTTGTCGCAATTGATTTATTTATCTCTGAGACGGCTGCACTCG11.25No Hit
GTATTCACCGTCTCTTCCTTAAAAGGCGGAACAGTGAACGTACCTTGGAA11.25No Hit
TATCTAAATAGTCCCTCTTGCCACCTCTCTCACAGTCCCTTAAAGCATCT11.25No Hit
CCGCAACAATGCCCGCAATAATGATTGCCAGCCAATAACGCGGATAGGCC11.25No Hit
CTGCGAAACAGATCCATCACGTAGCGTTGAAACCTCTATTAATCAATGGC11.25No Hit
ATCTGCAGAAGGGCCTCTTCGAGGAATATGCCGCCTTCGGGCGCGGCCAC11.25No Hit
GCTTACACCATGACGATGCTCAGGTCTCGCTGTCCCAGATGGAAAAAACA11.25No Hit
ATTTCCAGGTGCCCCAGTTCCGCGTTCGGTACCAGTTTCCCGTCGAAAAT11.25No Hit
GATGATGGTGGTAATGAAGTTGACCGCGCCGAGGATGGCGCCGAAGCCGG11.25No Hit
CATCAAAAAGTCCAGTCAAGGTTCTCATGTCACTTCTCCCAAATCCCGCG11.25No Hit
TTCTGCACGCGATCCAGCGCCCCAAAGTGCAGCACCACGGCGCGGTTCTG11.25No Hit
GCCCGGCACCTGCGTAGCGGCGATCAGGTCGTTATCGCCAGACGAGGCGG11.25No Hit
ATTCAAGCCCACGAGACCGAGCGACATCTCGTATGCCGTCTTCTGCTTGA11.25Illumina PCR Primer Index 11 (96% over 29bp)
ATCACCAGCTCCGCAAAATCATTGGGCGAAAAAGGCAGCCATTATAGGCA11.25No Hit
GCTGAGTCTTGGCTGCATCGTGCGCAACGATCCGAAACTGCCGGTACAGC11.25No Hit
ATATATATAAAAGTAACAAAAATATGAAAAAAATAAATAACTATCCGATA11.25No Hit
CTGGAAAAGCTCGGCATTCAAAAGGCCACGCTCCTCGGCCTGTCTCTTAT11.25No Hit
GTCGACGACAGCAACTTCAAATCGCCGGACGGCGTCAGCAGTCAGTTCAA11.25No Hit
GGTCGCGGCCGCACTCGTGAAAGTGAAGGAAATCCGGCCATCCGGCGAAA11.25No Hit
ACCTGATCACCGCCTACACCCCGACGTTCGGCAAAGCCGAACTGCACCTG11.25No Hit
GCTGTGACTGGTGGACAGCGACAGCACGATCGCGTCTTCGCTGGCGGCGG11.25No Hit
GCATGCTACTTTTTAATTGAGCTCGTTCTTGGCTCCCATGTTCTTTTACC11.25No Hit
GCAAAACAGCGGTCACCAGGTCGATATCCGTTTCGCCGATCCATCTGTCT11.25No Hit
GGGAATCTGTGCGCAGTCGTGGCGGCTGACCGGCAGCGGCAGCAGGCTGA11.25No Hit
GTGTGGTTGAAAGTATTCAGCGAGTAGCTGAACATGGTACAAGCTTCGGT11.25No Hit
CGCGCATCTCGCCCTCCGTGGCGATGGCCTGCGCAAAGGGTGTCACCCCT11.25No Hit
GGCTGGTTCAACGCAGGCACCTCGCTCGGCGCGCTGATCGCGCCGCCGCT11.25No Hit
GTTGATGATGGGCCTGCTGTTGTGCTCCCAGGGCTTTGCCGCCACCGCCC11.25No Hit
CCACCAGGGCCAGTCACATAACCGCCGAAACCGACGACGCATACGGGTTT11.25No Hit
GCTCAGCGAGATGGCGATGGATCCGCTGGATTTTTTCGGGGAAAACCCGC11.25No Hit
GCGTGGAAGTTACAGCAGCAGCTCGTCTGCCGGGATCTTGATCTTTTTGG11.25No Hit
CCGGGATTCCAGCCCCAGAGCACCAGTTTGTCTTCGCTGACGTGTTTTAA11.25No Hit
GAGTCCGAGGGTTCCCCGAAGAGGTGTCTCCACGGGGTGAGTTAAGGCTG11.25No Hit
ATCTGGAGCAGGGGGCGTATTCCCGCAGGGCTTGACCATTGGAAAGGTAA11.25No Hit
TTCTGGCTCTTGCAAGTCTAACTCAGTAGGAAGTCCGGGTTTCACCGCAC11.25No Hit
GGTCATCGGGCGCGACGACCTGGCGAACGACTCGGTACTGGCCAGCAATG11.25No Hit
GCACAAGGCAGCTGCTAAAACCTTGACCACCTGAGTCAATCCGATAATTG11.25No Hit
ATATTTGCTCTCTCGCATGGCAGCTTGCCTGCCTTGCTCAGCAAGCTCAT11.25No Hit
TCACCCATCTGCGGGCGAGCGCTCGCCGGCACGGTGGGCGGCAGCGGGAG11.25No Hit
ACCCTACACCCCTGCGCCTAATGGTCGCCGGGGAGTCTGTGACCTGTCTC11.25No Hit
GCGCTTGTTGTTGTACTTCGTATCGTTGTTTCAGTTCCAGAATTCCCCTT11.25No Hit
GTAGTAACGGTACGCAACCAGATAAATGGCCACGGCAGCGACCACAATCC11.25No Hit
GTGTGCGGGGTGAGAAACCGGTAGGAACAAGCCCGGCCGGACCGAAGTCC11.25No Hit
ATTCTGGATGTCACCGAAAAGTTGATCTACAAAAGTGGCATCGCTGCCAC11.25No Hit
GTACTCTGACCGCGTGATGGTCCGGCAGGTTCCGCCCGAGGTCCACCATC11.25No Hit
GAGCCGCCTGAGGCGCGCCGCACTGAGGGCACGCCAGCGCCAGCGTACTG11.25No Hit
GGTCATGCACCCCGGCCTGGGCCTGGAACTGCTGATGCTGGTGGCGCTGT11.25No Hit
CTGCACCGGCCTCGGCAGCCCCGACGGAGCAGCGCTCCTGACCGCGATCA11.25No Hit
CCATCAGTGCCGAGCCGGCACGCAGTGCGGTTTCGTAACCAATGCACAGA11.25No Hit
CGTCAGTACCGACCCAGATGAAAGTCGGCGGCCCGGGCACTTTATAGCGA11.25No Hit
ATGGGGCACCTCTCGAATCAGTGGGCAGGCTCCAGGGTTCTGTTTGAAAA11.25No Hit
GATCAATACCGGTAACAGGATCAGCAACGCGCCATTGACGCCGACCGCAG11.25No Hit
TCTGATGGGTGTCGCCGGTGATTACCGTCGACACCCATCAGACGGTCGAT11.25No Hit
CTGCTCATACACCCGAATTCCTGGTCGTGGGCTGATACTGCCGGCTCATG11.25No Hit
GATCTGGCGGGTGGTGCAGGTGATCCGCCAGGTCCGGCTGTGGGCTTCCA11.25No Hit
GACCAGATTGAGCACGACGATGGCGAGGAAGAGGAAATGCACCGGGCTGC11.25No Hit
CAGCCAATCCATTCCGTAGTGGCGCTGGCTGGGGGCGGGGTTCATCGCTA11.25No Hit
TCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT11.25No Hit
ACACCACTCTCGATACTCTAAATTCGATAGATTTTTCAATCTTGATCAGA11.25No Hit
CACCGGTACATGGTCGACGTGAAACCTTGGGCACATGGCTTTATCCAACA11.25No Hit
CGCCAGACCCTGCTGGAACAGGGCGGCCGGATCAAGGTGCCTTGCCTGCG11.25No Hit
AAATAGGCGTCCCGCTCGGCTGCCAGAGGGGCGATCCGGGCGCGCGTGGC11.25No Hit
CGGTAGTAGTGGTAGGGGTCGTCGTTGGGCAGGGGAGTTTGGCCCAGGGC11.25No Hit
GGGCATGGCCGAGGTAGACCGAATCCTTGACCAGTCGCGCGTACTGGCGC11.25No Hit
ATGCAGCAGCGCACCCGGATCTTCGGTCTTGGCCTGGCCATGGTCACGCG11.25No Hit
ATCGCGGGTTCGGCCAGAGCAAGGGCGATTTACCATCCGAAAGTAGTGTT11.25No Hit
ATATTGATACCTCCCTGGCAATGTCGCTGACCTCGTTCAAATCGGCCACC11.25No Hit
GAACGTGGTGGCGTTGCAGGGCAATCCGGAGCACTTCGACGAACGCCTGT11.25No Hit
TACCACAACCGTGGAGGCCGTGGGCCGATTGGGCGGTTAGCGAAATCGCA11.25No Hit
GAATATCCATGCCCCCCGCTTCCTGCGGGGTCACGGTGACATGGGAAATG11.25No Hit

[FAIL]Adapter Content

Adapter graph