FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n01_Tr4_C7I3a_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n01_Tr4_C7I3a_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16
Sequences flagged as poor quality0
Sequence length251
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTAGAAAGGTCGCCGCCAGACTGGCGCGGCGTTCGAGGGCATGCCATCC16.25No Hit
CTGGGAGAGGGCTGGGGTGAGGGCAACAATCCCCAGCCGAACACACCATT16.25No Hit
ACGGTGGTGGTCATACCGTAACAGTGCGCAATGACCACCACCGTTTCGCG16.25No Hit
AGACTACACATTACTAGCTGCAGCAGGACTAACTGGAAGCACTGGAGCTA16.25No Hit
CCGTGAGCGCAGCGATCCCGCAACTCAAGAGCGGCAAGATCAAGGCAATT16.25No Hit
TTTCAGCATCGTCCATGATCTGGCGACGAACCTCGTCGTAGCCGATACGC16.25No Hit
CAATAAAAATATTGGTCCGGGTGGTATTGTCTTCCACGGCGATTGGAGCT16.25No Hit
CCATGTACTTCGCCAGCCGTTAATCGGCTGGGTATGACAACGCCTCGGTT16.25No Hit
ACGATCAGGCGGCGGAGCTCAAGACTCTGCGCGGTCTGTTCTCGCTGCGG16.25No Hit
GGCACCTGCAGCGTGGGCGTGATTTCCATGTGGCCCACGTTTACCGCGAT16.25No Hit
GCAGAAGGTCTGGGTTTCCACATCCCGCAAGGCTATCTGTAGGCGGCGAT16.25No Hit
GCGTTATATCGGCGCTGTCGCAAGCGTCGGCACCGAAATCCTCAAGACGC16.25No Hit
CCATTACTTAGACTGCGCCCCGCCAACAGGATAAACGGAGAGTCAGATGC16.25No Hit
TTCAAAACCAGCCTGATGCCTCTGTGCTTCTATCAAGAGGGGATATTGTC16.25No Hit
TAACAGAAGTGCAGAACAAGCGAAACGAATCATAACGGACTTAGATGGCT16.25No Hit
GAGAAATGGAACGGCCCGGATCGACGCTCTGCAATCGCTCAAGGCGGCTG16.25No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)