FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n01_Tr4_C7I1b_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n01_Tr4_C7I1b_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88
Sequences flagged as poor quality0
Sequence length251
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAAAATTCACTCCTAATGAAACAACATAAAGTACAGAACTTGGAATGA11.1363636363636365No Hit
CTTCAACCGCGATGGTGCGACCGTGCATGAAGGTGTGATCCAGAACTGGT11.1363636363636365No Hit
AATGTACGCCGCACAAAAGAAGTGGTTGACCTTGCCTTAAAAGTAGGTGT11.1363636363636365No Hit
GTGGTCGCATTTATTTCTACAGAAAATATAGAAAAGCGTTTCTTCGCTCC11.1363636363636365No Hit
ATGTACTTAAATAAACCGGTACACCGCCCGCAAGTGTCGTCAGAGCCCCA11.1363636363636365No Hit
GCATGAAGAACCGGGGTCTCCAAGCGGTCGAGGTAGAAGCCTTTCAAGCA11.1363636363636365No Hit
CTATAATACAAAGGATTCGCTTGTCCGTCAATAGCAAGTTTTAAATTAAA11.1363636363636365No Hit
GTAGAAATCCCAGACCTCGATACCCGGCTGTTTCGAAAGGGCATTTTGCA11.1363636363636365No Hit
GTATGTAACCACATCGCATGGCGAGATTCGTTTGCGGCATGGCGGGGCCG11.1363636363636365No Hit
GTTCGACGGCCATGAACTCATGACGCTTCGAACGAGCCTCCTTGAAGGCT11.1363636363636365No Hit
GTCTATGCATTCCGTCTCCGAAAGCAGTCCCATCACCTCCTCCCACTCCC11.1363636363636365No Hit
GATCAGCGCGTTGCTGGAGGCCAGCGAATTTCTGGGTCGCGGCCTGGTGC11.1363636363636365No Hit
CCACTGACACCAGAATTTTTAACACGAATCATTCGAAAAGAACGTCCTGA11.1363636363636365No Hit
TCTCTTGGCTTACTGCCTTTTGCCCCAGAGATCATTCCTTCACGTTCCAT11.1363636363636365No Hit
GATAAAGGGCTCTAACTAGGGCTCTTTTTTTTGTTCCTTTTGGATAGATG11.1363636363636365No Hit
ATAAGGAAAGAATTTGTTTATCTCTTTCATCTAATGTTAAGTTTGGAATG11.1363636363636365No Hit
ATTAGACAGTGCGAGCATTGGCCACAGGAACGTGCCGTGTAGTTTTGAGT11.1363636363636365No Hit
ATCCAGGACGGCGCGGGTCGCCTTGAGGGGACGGGCAGAACGTTCGGGGG11.1363636363636365No Hit
CTTTTAAAATCGTTTCTTGTTTATTCATCAACATCACCGCCCCGTACTAA11.1363636363636365No Hit
ACACATTCGTCGGAGGAATATTTGTTTCTCCAAACCGTCAGTGCCGTGTA11.1363636363636365No Hit
GCCCTTCACCTCGAGCGTGGCGGTGGCGATGCCGGCCGCGCCCAGCAGCA11.1363636363636365No Hit
CCGTTCTACGACGTGCAGGCGATCATTGGCAAAGCCGCGCACGGCGAAGT11.1363636363636365No Hit
GAGCGTGACCTTGGCTACTGCGTAATCCTGGCCCTGCACCAGCGCCGGTA11.1363636363636365No Hit
GGGCAGCATCGCACCGCCCCCCAAGTGATGTCAGGGTTTATTTCATTCCC11.1363636363636365No Hit
CTATAAACCTTTAGCTCCTTTATCAGAGCATTCCCGACTTGTTTCTCATC11.1363636363636365No Hit
TCCCGGCGCTGCGACAGGGCGCCCGTCGCCGGATCGCAATCACTGTCTCT11.1363636363636365No Hit
GTTTACAAGGGATACAAAAATCTGATCTTGATGATCGACTTGCTCAATTT11.1363636363636365No Hit
ATGGCTGCAAGCATGGAAAAAGCAACTGCTTCTTGGGCTAAATGGGCGGC11.1363636363636365No Hit
AATACATCCTTTTTCGATTGAAAACCGAGAACATGCTTTACGATGACGCC11.1363636363636365No Hit
GATTATGATGCGTGGCTGATCAAGATCGGTGAAGGCGACCAGTTCGGTTC11.1363636363636365No Hit
GGTCTACCTCGAAAGGCAGCCGGTGGCCCGGCGGCCATTCTCCCGAAACG11.1363636363636365No Hit
ATGAAAGCGAATATGCCAACCCCGACTGGGATCACGCCGCCCGCCGCGTG11.1363636363636365No Hit
GTTTCATTCAGACGATCAAAAAGAAGCGGCGGCAACTAGCGAGGCAGCGA11.1363636363636365No Hit
GCATCTGGTTGTTGCCCTGGCCGGCGAAGTTGCCTTCATTGCTATCCTCG11.1363636363636365No Hit
GCACACGGGTCGTCTCACCGACGCCCGGCTTAATCAAATGTGTATTGTCT11.1363636363636365No Hit
CCCATATACGGGTATACCTTGCGCCTTTAATGCTGGGATTGTCTGTTGGA11.1363636363636365No Hit
GATGAGCCCTCTGTAATCGATGACATAGTAAGAGCAACAAACAGACAGTC11.1363636363636365No Hit
GACAAGAACGATGCCAATGAGGATCGCAAGCGCTGCGCCCATAATGCCAA11.1363636363636365No Hit
TGGTAAAACAACACTTCTTCGCTTGATTCAGCAAATCCTTCTTCCAGATG11.1363636363636365No Hit
CACCTAGATGCTGAAACAAAAAGGAGGTTCTGGTTAATGACACAGGAAAA11.1363636363636365No Hit
ATCAAAAAGGTATTCAAGCGAGATATCAGGTACTTTCCTATCCGACGACA11.1363636363636365No Hit
GCCATGTAGATGATCGAGATCAAGTGGACTCACCTGCCTCTTTTTCGCCA11.1363636363636365No Hit
GGATAACTACCACATCAACAAAAACCAGCAGCATTGGGAACTGGTCAAAG11.1363636363636365No Hit
GTCATAAGAGCGGGCATAGATGCGCACGCTTGGAAATTCTGACTGGATCA11.1363636363636365No Hit
ATATGAGGAAGCCCGCAAGATCGGCGACCCCGTGGCGCTCGAGCGCGAGC11.1363636363636365No Hit
GGGATATTCAGGGCACGCAGAAGGAAATTTCCGGCCGTTACGGCACGCAG11.1363636363636365No Hit
CCCAAATCGACCAACCGGTGGGACCGCGACGGAGCAGCTTTACAAGGGGG11.1363636363636365No Hit
GTGATGCGGGCAGGATTATCGCCTCGCTGCTAAACTTTGATGACGTTTTC11.1363636363636365No Hit
TATAGGTATCGCTGACGTTACCCACTTCATCGATGAAGGCAAACAACGAG11.1363636363636365No Hit
GCGTGAGAAGGAAAAGCAGGCGTGACGGGCGCGCCGTTTCTGTCTTGCTC11.1363636363636365No Hit
CTACTGGAACGATCATTATCCGGATTCGCACCTGTTCGCCGACGCGGAAA11.1363636363636365No Hit
GATCCACTGTTCCTTTTGGTCTTCCATCTTCAGTGAGCACCCTCTCTTTC11.1363636363636365No Hit
GAAGAACCCGGCTTTTTCATTTTTGGGGTGGTGCGTGCGGAGAAGGTGCT11.1363636363636365No Hit
GGCTTTGGCGGTTATTCTGGAGGCTACTGCGGGGGCTACGGTGGCGGTTT11.1363636363636365No Hit
CTATACTAGAAGGAATACAGGCATGACCAACACCCGGTATATTGCATTGC11.1363636363636365No Hit
TTCTGGAGAAGGCCGGCGGTCGTCTGGTCGGGCTAGATGGAAAACCTCTG11.1363636363636365No Hit
TCTGGGCGACGGCCTGCGCGACGCGCTCGATCCGCGCGAAGCATGATTTC11.1363636363636365No Hit
GGTCACGAGGTCCTTGAACGACTCGAACGACTCCTTGGCGGCCTTTTGGT11.1363636363636365No Hit
GACAACTTCAATGCCAAGCTTTTTCGCTTCTTTTGTCACACCGTTTTTAA11.1363636363636365No Hit
GGAAGATTATGCGCTGCGCTTCACCGCTAAAAGCGCCCGGCAATTTTCGC11.1363636363636365No Hit
AGTTTTATCGCTATGCCATTAGTCCTTTAATGGCCAGCCACTGTCGCTTC11.1363636363636365No Hit
TTGGGACAGTGGTCGGTATTCTTTGTTTTTTCATGGCAAAACTATTAATG11.1363636363636365No Hit
CCTCCTGTAACAAGCAGGTGCTCATAGTCCTCGATGAAGCAATCCACCGA11.1363636363636365No Hit
CAAGGTCGACTTCCCCGCGCCGTTCTCACCGACGAGTGCGTGGATCTCAC11.1363636363636365No Hit
CCCCTGTTTGAACAAACCCAAGCTTTTGATAAAATGCCATGGCGGCTTTA11.1363636363636365No Hit
GTTCTGAGTAGGCAGCGGTTTCATTTATACAGATGTTGTATGCTATAATG11.1363636363636365No Hit
TGAGAATATATGTGTGAATGAGATAACCAATAATGAACTTATATAAGAAC11.1363636363636365No Hit
GTATATAGCCGATCGGTATTTATGTAATTGCACAAATACCGCTAGGTTAT11.1363636363636365No Hit
CCTATTGTGAATAAAAGCTCTTTGTATTTTTCACAGTTTGTACATCCCAG11.1363636363636365No Hit
CTATATGGATCATAGGGGCGAATCGTTTAAAACGAATGGCTGGCCAGCTC11.1363636363636365No Hit
AGGTCGGCTGCGACAACTTCCTGTCCAAGTTCCAGCCGGACAAACTGGTC11.1363636363636365No Hit
GTGTATCTGTTTCCCGGTGCGCCGCGCACGCTCCGACTGGGTGTGAGCAT11.1363636363636365No Hit
ATGGTCAGCAGCGCGGCGACAAGTGTCGTCTTGCGGCCGACGCGGTCACC11.1363636363636365No Hit
CACCCAGGCTGTGCCCTGCTGCATAATCTGCTTTGATTCCGCTCTCTTCA11.1363636363636365No Hit
CATGGAGCCAGTCAACCAACTGCTGAGACATGAATAGACAAAGTAAAAAA11.1363636363636365No Hit
CTGTCTTGACATAACCGTCTTCAATGACTGGCTTAACACCATTACCTGGT11.1363636363636365No Hit
CCATTAAGGAAGATAGATCGAATTTGGCGATTTCAGGCAATTCTTTTTTC11.1363636363636365No Hit
TTATAGAGGTAATTATGCTATGCATGAACCCAATGGAGATACATGAAATG11.1363636363636365No Hit
TCCTGGCACCATGGCTTGCAGGAGCGGCAATGGCCTTTAGTTCTGTATCG11.1363636363636365No Hit
ACCATGATCAGTAATACTTTGTAAAGAGGATAAGCCTACTAAAAATCCAT11.1363636363636365No Hit
GGTCGCCTCGACGCCGTCCATTACGGGCATCTGGATATCCTGTCTCTTAT11.1363636363636365No Hit
TCGCTACACCCATAGCAAATATTAAAGCCAAATTCGCAAAAATTGCATTT11.1363636363636365No Hit
TGGCGAGCAGGCGCACCTGGGTTTTTGTCAGGCCTGACAGCGGGGCCAGA11.1363636363636365No Hit
GTCTGCACCATCATCCGTGATGGACGCACCGTCGTCGATCGCGCCGCGAC11.1363636363636365No Hit
CAGGAACACCGTCCCGTCGCGGAAACCACGGAAGGTGATGTCGCCGCCAT11.1363636363636365No Hit
GGATGCAGAGAGGATCGGTTAGCTTGGAAAGCGTGAATTCGCTCATCCGC11.1363636363636365No Hit
CCTCTTCATCGACCACTTCCGACAGGCACGTGACCAGATCCTGCACTTTA11.1363636363636365No Hit
GTCTCCAGCACCGCATGCAGCAGCACATCGCAGCCGGCTGCTGCCCATTC11.1363636363636365No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)