FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n01_Tr4_C5I4c_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n01_Tr4_C5I4c_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98
Sequences flagged as poor quality0
Sequence length251
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAGGAGGTGCGCCATGCGACATGACCCTGCCAGCGGCGCCCTCGTGAT11.0204081632653061No Hit
GAAGCCGGCGGGCGCCAGCACCTGCACCTTGCCGGCGGCCACGTCGGCCT11.0204081632653061No Hit
GCGAGGGTTCGGGCGTGGGCGATCAATGCTGCTTTTGATGCGATATAGGC11.0204081632653061No Hit
ATATAAAAGATCTCGATCTAGTGATGGGTATAGATCAACAATGGCTTTCG11.0204081632653061No Hit
GGGAATTGATTCAAAAGCAGCAGACCGCCTTGCAAAGCTCATCCCATCAA11.0204081632653061No Hit
GTACCGGTCAGCGATACGCCATTGCTCGGTTGCACGGTCGGCACGACGGG11.0204081632653061No Hit
GTCACAGAGAGTGTGACACTTCAGGGGCAAAGGTATCAGGCCCCTCGCTC11.0204081632653061No Hit
GTTCAGGTGTGTGCATTGTGTTTCATCCTTTGAAAGACACGTGGCCGAGC11.0204081632653061No Hit
GCCAAACTTCCTGCAAAGGTTGTCGTTGTGTCTCCATGAACAAGCACAAT11.0204081632653061No Hit
CATCAAACACAGCTCTCTTGAATATCGATCCCCTAAAGGAACGAGCAGCG11.0204081632653061No Hit
TAATAAGGCAGCCGTTCCATCAATTCGGCGCCTTTGATTATTTGTCTTGT11.0204081632653061No Hit
GTACTAACAACTCGCGTTACTACTAAGCTCGCGTAGCTTCGATCTGAGAA11.0204081632653061No Hit
TCTCTAACAGGCTAATAACCAATCCTCCTGGAGATGTCGCAGAGCTAATG11.0204081632653061No Hit
TCAAAAGACTGATGTACCATAAAAATGAGCATATAGATCGTTCCACACAA11.0204081632653061No Hit
ATCATTGTCTAGGATTATCAAACATGAAACATCTGTTATTCCTGAACAAG11.0204081632653061No Hit
ATTTCATACATCATTTGTTTTCTTTGTTCCAATCATTAAACCCCCATTAT11.0204081632653061No Hit
GTCTTATATCTATTAAATGGACGTTGTCCATCTCTATATCAAAAACGCTT11.0204081632653061No Hit
GAAAAACGCATGACACGAGTCTACCAGTTGATCATTTTCGCCAGTTTCTT11.0204081632653061No Hit
ATAGTGGACCATTTCTCAGGAAATATTTTTGGACGCCAAATGCCGGCTTC11.0204081632653061No Hit
GCATAGAACTCGCCCGTCATGTAGGTGCGAAAGCCCGCCACGCGGCCCTT11.0204081632653061No Hit
CATACAATTCGCTGATTCGTCTCACATTGAAATCTGTTCGTGCTTGAAGC11.0204081632653061No Hit
GCGTAAGAATGCAAGCTTTGATATTGATCGTATTTTAGAGATTGAACAAG11.0204081632653061No Hit
GAATGGGAGCCTATGCCGATGCCTATGCAGCCCCTGTTCGACGCAATCGC11.0204081632653061No Hit
GTGTTTGATTGGAAAGAAACTGGCGGTATGGTGGAGGCTTCTGGCCATAT11.0204081632653061No Hit
TTGATACCGAGGATGCCCTTGTACTTCGCCGCCTGAACCCGCGACAGCAG11.0204081632653061No Hit
ATCCTATAGAATGCTTCATAAACTACATATTTTACGGGGTATTTTCTCTC11.0204081632653061No Hit
CACAAGTTCACCAACGAGCTGGTTCGCATAAATGTGTCCAGCAATATGAT11.0204081632653061No Hit
GCCCGATGCACCCGGTGTGTACGTGGACGGCGCGAAAATCGCCTCCCTCG11.0204081632653061No Hit
CATCTACGACTACTACAAGACCAATGGCCACAAGACCGTGGTCATGGGTG11.0204081632653061No Hit
CCCTATAGCTACTTACAACTCATATGAACTTGGCAGACGAAAGATTCATG11.0204081632653061No Hit
GTACGGAGCAAACGCAAGAGTCAATGAGCTGAATGACATGATCGAGCAAA11.0204081632653061No Hit
ATGGAAAGAGCGGCCGATCGGATACATCAAAGATCCACTCACTATCTGTT11.0204081632653061No Hit
ACCTGTGTGTGAACCTGGAAGGCGATGGTGTGCTGACCCAGATGGTCAAG11.0204081632653061No Hit
GAATGTTCAGAGATGGACGCAGGTTCAATTACCAGCTGAGGGTTTGCCCG11.0204081632653061No Hit
AATTAAAGGGGGGATTCATTGTCTTGAAACGCTTGCCACTGCGCCTAGAT11.0204081632653061No Hit
TCCCCATTGCTCGAATTAGACTGCGGGATAAAGGTTTTTCCGTCATATTG11.0204081632653061No Hit
GGCTTCCCCCAGGCTGGCCAGGCAGAACAGGCCATTGAGCGGCTGGCCGA11.0204081632653061No Hit
TCTTGAAGGAGTCGTCGGTTTTCAGGTCTTCGATGGTCTTGGCCTTGACG11.0204081632653061No Hit
GCCGCGGCCCAGGGGCGTGTGCTGGCCGCGGCAGCCGGCGTGCCCCATGT11.0204081632653061No Hit
ACAAATTGGAAGTGTGATCCAGCTATTAGAGGATGGCAACACCGTCCCGT11.0204081632653061No Hit
CTCCTTCTTCCCTGCGTCCAGTCGAACCAGCGCCCCCAACGGCAGCGTCA11.0204081632653061No Hit
ATTGAACATTTTATAAAACGTCTGTTTGTAAGCGCTTTACAAAAGGTTCT11.0204081632653061No Hit
GACCTGTGGGACATGGACGTCGGCAGTCAGCCAACCCTGCTGGACATGAA11.0204081632653061No Hit
ATGTTCACAATATCTCCTAAAAACAGACACAATTGCATCCTGACCTCTCA11.0204081632653061No Hit
GTGATCGCCTTCGATGACCTGGGGCGTGCGCCGCTGCTCTATCACCAAGG11.0204081632653061No Hit
GTAGAGGGCGGCACCGCCACCGGTGGCGCGCGGCTCGACTTCCTGGCGCG11.0204081632653061No Hit
ATCTAACATGGAAGCAGAGGAATCCAAGGCCGTCCAGCTTCTCATGAATT11.0204081632653061No Hit
GTATTATAATCATTGAGGTCAATTTTCCACTGACCATCTCCGTTACGTTT11.0204081632653061No Hit
CAGCACCCTTTGCGTTGAGCCTGCTCGTGCTGGCCGGCTGCGCGGTCGGG11.0204081632653061No Hit
TCTCCGTTTAACTGCCCAATCAGTCCTTTGTGGTCTGCCAAAGCAAACAT11.0204081632653061No Hit
CATCTGTTCATAACCCTCGCCCAGCGTGCCGGCAATCACAATGACCGGCA11.0204081632653061No Hit
GGGCCGGACCTGCTGCAACGGGTGCAGACCACCAGCATTCGCAACCTGGA11.0204081632653061No Hit
GGTATCTTCTGTCAACTATCAAGCCGATCATCCAGTGTCTGACGACATTT11.0204081632653061No Hit
GACTAAGGGTATCGGTAGATCTAGGGATAGTGATGTTAGATTGGGTGTGC11.0204081632653061No Hit
CTCGAAGCGGGCGAAATAGGCGGTCAAGAATGGACCGAACATCGCCACGG11.0204081632653061No Hit
TTTCAGGATATACCTCGTGCTGTGGATGTGCTGAAACGCCGCTTGTTGCT11.0204081632653061No Hit
CTCCTGAGCCGTTAAAAAAATACCCTCCGGCGCCGTTTGCTATAAATTCT11.0204081632653061No Hit
GATCACCGCCGGGTCAAACACGATCTCGATCGCTTCCGCATGGTTGCCAT11.0204081632653061No Hit
AGGTACATCAGCATGATGAGCGCGAGCACGCCGTAGCGGCCGATGCCCAG11.0204081632653061No Hit
GGGCGCCTCGGTGATCCTTTGGATCATTGGGGCTACCCGCAGCAGGCGAT11.0204081632653061No Hit
CATAAATAGACATAAATAACGCTTCAATAAAGAAAGCATAAATTTCTATT11.0204081632653061No Hit
ATGTCAACCATCGGGCACGGGACTTGCACGAACCCGCTTGCGACATCCCG11.0204081632653061No Hit
TTCCCACGCAGAGCGTGGGAACGATCATGCGACAGTTCAGAAAATCAATA11.0204081632653061No Hit
GCACCGGCGCGCCCTGGAACAGGCGGAACTTGTACTTGCCCGACAGCTTG11.0204081632653061No Hit
CCGCCGTTTCGAGCAACTGACCGATCGCTATCAGGCCTGGGTTGGCTATG11.0204081632653061No Hit
GAAGTGTATGAACAAGGAAATACATTTAAGGTCTCAACGAAACGCTCTTA11.0204081632653061No Hit
CCCGTGCCTTCAACCTGGCGCTCAAGCGGCACCTGGACGAATCCGGCCTG11.0204081632653061No Hit
GTTGAATACCGTACAGATGATGCACGCCTGACAATTGAAGTCATGAAAGA11.0204081632653061No Hit
CTGCTCGACGGCAACGTCGAGGCCGAGGGGCACGAGTTCTTCTCCCTCGG11.0204081632653061No Hit
GGCTATCGGATGGCGCCCCCCCCTCATCCTCACCCATAACGACTGCGGCA11.0204081632653061No Hit
GCGGCGGTTACCGGTGCGTTTTCCCTGGCCAATGCCAGTCTGACCTTCGT11.0204081632653061No Hit
CTCTAATACTTCTTCTGATTTCTTCACAGCGGCAATCTTCGTTTTACCGC11.0204081632653061No Hit
GGTTTGTACTGCAATTGTTCAGTCATCATATAACCCCCGTTTCGTTCAAT11.0204081632653061No Hit
GATATGATATTCCTCTCGAATTTACTGGCAAAACAAATTCTGACTATTAC11.0204081632653061No Hit
CAAGATGAGTATCAGACATGGCTTCCCCTTTATGATCAGAGAAATATTCA11.0204081632653061No Hit
TCTGTAAGTAGATTAACACATATGAAATACCATATCAAGCATTTTAAAGG11.0204081632653061No Hit
GTGTACTCGGAGAAGGTCGAGGTGCCCATGTAGTGGAAAATTGGCTGGCC11.0204081632653061No Hit
TTGATAGCCTTCGGCAAAGGCCGAAACGTTCAAAGCCACCACCGGGAACA11.0204081632653061No Hit
GTATGGAAGATCCATGGCACTACCGGAATAAAAGTCAGTTCCAAGTCGGA11.0204081632653061No Hit
GGGCAGCATGTCGTGGTCATCTCGAGCGACCGCGAGCTGCAGAACCGCGT11.0204081632653061No Hit
ATACCAAGCGATACTAGGAGAGGAAAAACATCTGTCCCGGTTAGAGTATG11.0204081632653061No Hit
CTATTTAACGGCTAGAGGGATTACAAATGAAGTGTACTATACTGCGGCGA11.0204081632653061No Hit
GTATTAGACGAACCGATCCAAAATTTTTTTGTGAACAAAATATACGAAAC11.0204081632653061No Hit
CACCTGGACGGCGAGCAGGTGTACCAACTGCGCAAAGTCATCGAAGTGGA11.0204081632653061No Hit
TGACATGACAGCGTCACTTAATATTTGGCCAAGTGGTCTTTTTTCTGCGA11.0204081632653061No Hit
AACTGGCGCTCTGACGCCCCGAGCCCACGAGACACGCTACTATCTCGTAT11.0204081632653061No Hit
ACGGTCGGCTGCTGTTGCCAGTCGCGGTAGGTCACCGAGCTGGCGAGGGC11.0204081632653061No Hit
GTTCATCGATACAGGCGTGCTGAAGATCCGTAACGCGTTTCATCGCCATG11.0204081632653061No Hit
CCACAACCCGATCTGCTGACGCAGATCGGGTTGTGGTTCATCCAGGCGTA11.0204081632653061No Hit
GAATTAGTATTGCTTCTGTGGATAATAATTATCTTACTGATCATTTTAAT11.0204081632653061No Hit
ATCTCGGTGCGGTCGTATGTGAGCGTGATGCTCTCGGCTCTGAGGCGCGA11.0204081632653061No Hit
GAAATAACGTGATCAAACCACACTAAACGGTAGAAGATATGTTCGAAGAA11.0204081632653061No Hit
CGGTAGAGCAGGGGATTGAAAATCCCCGTGTCGGCGGTTCGATTCCGTCT11.0204081632653061No Hit
TGCATGGCCCAGACGAGCTCGACCTCCTCGTCTTCTTCCTGGGCGTTGCG11.0204081632653061No Hit
GTTTAATATCTACGCTGTCGATAACAGCAACAATCGTAAAACGTACCACA11.0204081632653061No Hit
GTTCTGTATCTTTTTTTTTTTCTGAAATACAAGCTTTATGTTAAATTTAA11.0204081632653061No Hit
TGTTATACCTGATTCTCCAAACGGAAAAAGTCGGCTAATGCTTAAATAAT11.0204081632653061No Hit
GCTGCAGGGCTTCCAATTGCACTGGCGTCAGCAGTTGCAGGCGCAGGATC11.0204081632653061No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)