FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n01_Tr4_C5I3c_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n01_Tr4_C5I3c_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42
Sequences flagged as poor quality0
Sequence length251
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAGAGGAATGGGCGGCTGAGGGGAAACGTGCGATTGGAGAAGGAATG12.380952380952381No Hit
TCTTTCACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAG12.380952380952381Illumina PCR Primer Index 1 (96% over 29bp)
GTTCTAATGGCCTTGTTTTCATTTTAAGCTTCCTCTTTTATGGGCACATA12.380952380952381No Hit
GTTCACGCGCGGCGGCGGTGCGTCGGACAACAGGGGCGGCGAGCCGTCCA12.380952380952381No Hit
GCTCATCAGCGAGGCGCACCTGCCGAATGAAGAGCTCATCACCATCACCC12.380952380952381No Hit
ACCTTGGGCTTGCCCACGGTGCTGTCCAGCGCGTGCAACTGGCGGTTGCT12.380952380952381No Hit
CTCCAGTCGATAGTCGCTGGTGGTCAAGAGCGAACTGTTGGTGATGTTCA12.380952380952381No Hit
CAGCAGCGCGTGTTCGCGCAGCCACTGGCGATCCGCCTCATCGAGGCGCA12.380952380952381No Hit
CAATATGTCCAGGATCCTACTAGTGTTGATGAAGACATAAGAGGCATATT12.380952380952381No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT12.380952380952381No Hit
TCCTAAGAATGGCCCAAGCTTTTGCATGATGTTGTCAAATCGTCCATTTG12.380952380952381No Hit
GTACTACCTCGCGGTAAACCTCTCGGCCCGCGCCGGCGAGTTTGCCGCGC12.380952380952381No Hit
GTCTGGTTTAACGCGGCGCCCTTGAGTGTTTCGCGAGCAAGCTCGCGCCT12.380952380952381No Hit
GTCTTGGGATCTACCGAGAAAGAAAAATGGTGGCCTGGGGCGGAATCGAA12.380952380952381No Hit
TCTTGGCGCTGTTAACGTACCTATGATCGGCAGGACAGAAATACCATCCT12.380952380952381No Hit
GGCCGTGGCAGTCCCAGCCCGGCACGTAGAGCGCGTCGTAGCCTTCGAGC12.380952380952381No Hit
GATTACTACTTGGTGTATTTGGCTCGATTATACCAATCGCTGTATTGCTT12.380952380952381No Hit
ATCCATGCGGAGAAGAATAGCCCCAATCTTTCATATTTAAGCGCTGTAAT12.380952380952381No Hit
CGATCCTCGAGAAAGACAGCATCGATATGGATAAAGTGTACTTGAAATCA12.380952380952381No Hit
GCCCAGCCCAGCGCGTCGGTCAGGCGGCGCGACTCGCGCATGCCCTGCAC12.380952380952381No Hit
GTCGAGCACTGTGCCGGGCACGGCGATGTGGAAGTCGAGCACCGCGGCGA12.380952380952381No Hit
TCCATATTCTAAATGGAGAGGGCCAAATCATTGGAGAAGGTAAGACAGAC12.380952380952381No Hit
CTGTCAGTGTGTCCTTCCACACTTTTCGTCCTTCATGTCCTTCTGGTAAA12.380952380952381No Hit
GGAACTGCGCGCGCCCACCGAACAGGAAGCGGCCTCCCTCGGCCTCACCG12.380952380952381No Hit
TATCCGCAGCGTCCATCACGGTGCCGGACTTCGGCGAGCGAATTCTGGAT12.380952380952381No Hit
GTCCACCATGTGCACGACCGAAAACGCCAGATCCCGATTGACGCCCTGTA12.380952380952381No Hit
AGTAATAACCATCTCCCATAATGTTACTGCCTCCTTTTTCATCAGTTCTG12.380952380952381No Hit
GGGTGCCTTCGTTGTGGATGTCCCAGCGCTCGAAGTGCTGCAGCGGCGCG12.380952380952381No Hit
GCTGCGCACCGCGCTGGTGTTCTCGCTTCGGGTGTCGGTACTCTGGCTGC12.380952380952381No Hit
GTTGTCCGGGTGTGCAACGACGATAAGGGCATGCATGGGTTAGCTCCAAT12.380952380952381No Hit
ATTAAGATGCAGACCCATTTGCCTATTTTTGGCATGTGGGTTTTTTTTGT12.380952380952381No Hit
GTTTTTTGATGATTTCAGCAGTTTCTTTGCCAAGCTCGTCAGCAAGCTGG12.380952380952381No Hit
GCGTTGGGGGGGTAGCTCAGCTGGGAGAGCGCCGCGTTCGCAATGCGGAG12.380952380952381No Hit
GGCGAGCACCAGCGCGTGGAAGACATCGCGGCGGAGGTGGCGGCGGCACA12.380952380952381No Hit
TTCAAAAGCCCAATGATGTTAAGCCCTTTTGCGAGTTTAATCGTGTATTC12.380952380952381No Hit
TTCTAAAATACAGTAAAAGGACAACATTTATTCCAAGAAGCCCAAGACAA12.380952380952381No Hit
ACTTCGCCTGGTTCGAAGAGCCGATGATGGAGGAGTCCGCCGAGTCCTAT12.380952380952381No Hit
GTCTCCCACAACCCGGGCCGGTGCCGCGCACCTTCCTATTTCCGATGTTC12.380952380952381No Hit
GATTTGAGTCATATCCGCAATTTCTCCATCATCGCCCACATTGACCATGG12.380952380952381No Hit
CCGGGGGCCTGGGTGGAATGGTCGAACTCCAGCGGGATCGCATAGGGCAA12.380952380952381No Hit
ATTCTGGAGCTCATTCGAGAGCTCAATGTCGAGCATGGATTAACCATCGT12.380952380952381No Hit
AATGAAGAGTCGTAAAAGGCATCTGATGTTTGCTAATATGTAAATAACTT12.380952380952381No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)