FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n01_Tr4_C5I2c_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n01_Tr4_C5I2c_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126
Sequences flagged as poor quality0
Sequence length251
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAAACAATGCGCGCCACTCAAAAGTGAACGTCACCCTTGTCGGGGAGA10.7936507936507936No Hit
GTTCGGCGCTCTGGCTCAGGTCGAACACCGACAGTGTCTCGCCTTCTTCA10.7936507936507936No Hit
GGGTTTATGCGGGGCCCCATCGCGGTCGCACGCGGGTACACGCGTGAACA10.7936507936507936No Hit
GCTGTCAGGGAATGTAGAGCGATTATTACATAAAGGGAAGTGAGAAGATG10.7936507936507936No Hit
ATTTGGCGCAGATAACCGGAACTGTATTTCCTTCTTATGAAGCGATTCGC10.7936507936507936No Hit
GCAGATACAAGTGCCAGACCAAAGGTGATCATAGGAAGATGGGATCTATT10.7936507936507936No Hit
CGACCGTCCTGAGCACGAAGGCGCTGATAGCGCTGATAGTGATGACAGCG10.7936507936507936No Hit
GGATTGCAGGGCTTCGGTGGCGTTGCGGGTGAAGGCCACCGCTGCCGGGT10.7936507936507936No Hit
GGTTAATATCACTGCAAACTTGATGGATGAAGATTGATTATTTTTTTTAC10.7936507936507936No Hit
GTCACCGTCAGAAGGGCAGAGGGGAATTGTGCGAATCGGGCTCAATGTCC10.7936507936507936No Hit
CGTGAGCTCTGCACTTTTCAACTCTTTTTGAAACCCAATGGTCATCGAGC10.7936507936507936No Hit
GGTTCGTTGACTGGGGCTCCTCTGTTTTACCTGAAGGAATTGCACCAAAT10.7936507936507936No Hit
CCTCCCCCGCCGTACGCAAGCACGCATTGGACGCGGGGATTCAACTGCGC10.7936507936507936No Hit
ACCGTAGTCCATCAGCGCACGCGCCGCCTCTGCTGCATACCCTTGGTTGC10.7936507936507936No Hit
AATTGATGGTCCGGCAGCAGGCTCAGCTGTGCGGCGAAATCATCGCCACC10.7936507936507936No Hit
CATGTGATTTACCTTTCATAAATCAATAAGTTACCTAATTTAACGTTTCC10.7936507936507936No Hit
CTATCGTGTACAACATTGCCGCAACGATCTTCGGGGGATTCTCCCTCTTC10.7936507936507936No Hit
GTATTCATCATGCGATACCTTTCGGTGGGTTTCCCCATTCGGAAATCTCC10.7936507936507936No Hit
GCCTGGAAGTGTGCTCGGCGTTCGTCGAGCCCTCGCTGGCAAAGGCCGAG10.7936507936507936No Hit
GACCGACATTGAGTACATGGACCGCGCCGAAGCCGCGCTCGCCGCCATCG10.7936507936507936No Hit
GTTATATACGGTCCCTTGAACGAGCTTTACGGCGAGACCTGCCTCTTTTA10.7936507936507936No Hit
GAGCACCAGGGAGACAGCGTTGAGGGTGGTGAGGTGGGAGTGGAGCTGTC10.7936507936507936No Hit
GACGGAGAACAACGTCACCCCGTACCGCCAGACAGTGACCGACAACATCG10.7936507936507936No Hit
CCACCACCTGCTGGCTGTCGTTCGAGCGGCTCGTGCCGGGCCGCGACGCG10.7936507936507936No Hit
TTCGTGACCTTACCACTGATGAAATTGAACAAAAAGTAAAGCCTCTTAAA10.7936507936507936No Hit
ACATAAGCCAATGGCAACAAGACCAATGGTTGTGCTGCGCATGCAGATCC10.7936507936507936No Hit
GTGTTGGGATTTGTGACTTGACTCAGGCTGAATTATCTTCAAACGGAACG10.7936507936507936No Hit
ACTGAATGCTTGCTTTAATGATCTTTTCTTTTTTTTCGTTCATGAAATAA10.7936507936507936No Hit
ATTTGAAGGCATGCACATTCCAACAAAAGAAGATTGGGCAGCACTCAAAG10.7936507936507936No Hit
GGGCTGACATCAGCCCTTTGAATTTGCTGTTAAGCAGGGTGAAGCTGGCC10.7936507936507936No Hit
GACCTCCCTGCAATTGGTCAATCTGGCGGACCGCGGTCTTTACATGGCCA10.7936507936507936No Hit
AGCCAGGCTTGCTGCTCGGCGATGCAGATGTCGGCGTCATTACTGTCGCT10.7936507936507936No Hit
CCATTATTTCCTCATGACCTTATTAACCTTAGTGACCGTGGCATCCCTGC10.7936507936507936No Hit
AAAGAGCATCAAGGCGGGCAGCCTGAGCCACTATCAGGCAGTAAAAAAGT10.7936507936507936No Hit
CTGAAAATAGAGGACACCAAGCTCGATGCCGCACTGAAAAGCAATTTCGA10.7936507936507936No Hit
GCGCAGTGGTTCATCGGCATGGACAAAGAGCCGGTCAGCGGCGACACCCT10.7936507936507936No Hit
TCAATACTTTGAACGGGTAGGGGTCATTAACGGTACCGTCAATGGGGATG10.7936507936507936No Hit
GAAGGACCGTCTGCAAGGATTTCTCCTTTGACGACTTCATCTCCAACACT10.7936507936507936No Hit
GGACAGTGTGGTCAAGCTGCTCACCATGCGCCACGGCAGCAAGGATTTCT10.7936507936507936No Hit
GTAAAGGTCAGCTTTCCTTCAACAGGCGTCCTAGAAAACACGATGCCTCT10.7936507936507936No Hit
ATACCCTCGGGGTGCATCGTTCATGAGTGCATCGTCCACCCCTTCCAGCG10.7936507936507936No Hit
GGCCATGAAGAAGTCGGCGAAACGCGGCACCATGCCGCCAGCGATGAATA10.7936507936507936No Hit
GTCATCGTCAGCTCTATATTCGGACTCCCTGTTCCCCTTACTCAAATTAC10.7936507936507936No Hit
CCATCAACTGTACATCGGACAGCGGACGATAGAGCAGGCAAGGCCCCTTC10.7936507936507936No Hit
CACCAAGGGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA10.7936507936507936RNA PCR Primer, Index 20 (96% over 28bp)
ATAATAAACACCATCAAAAGAGATGCGATAAGAAAGGCGGAGAAAAAATT10.7936507936507936No Hit
GGGCATTAATTGTGTACCTCGTGATGCTCTGTTTAGGTGAACTGTCTGTC10.7936507936507936No Hit
ACCATATACAGGTACGCTTAAGTCGAGTGCTGGGTATGGCAAAGTTGTTG10.7936507936507936No Hit
GTCCAAACCTCCGTTGAAAATGTCGCTTCTTCATCAGAAGAACTGACCGC10.7936507936507936No Hit
TCTTTTACTGATTTGTATTTCTTAGAGTAGATACCGAAAGGCTCAAGGTG10.7936507936507936No Hit
CCCTGGCCCGCGCCAGTGGCTTGCAGGTGCGGCGCATCAGTTGCCGTCGC10.7936507936507936No Hit
CTATTTGAATGAAGAGAGAGTGCGCCCTCCTGCCGCTTTTTGCAGACGGT10.7936507936507936No Hit
CTTGGCCACATCCATAATAAAATCTTCTTTGCGGCAAACGGTAATGTTCG10.7936507936507936No Hit
GCTGTGTCAGGAACGATCGGATTTGTAGGATTGGTAATTCCACATTTTAT10.7936507936507936No Hit
TTCCTGATCAATCTGCCCGAGATAGTTCAAGCTGATTTCAGGTTGCACGT10.7936507936507936No Hit
AAGTAACGTGGATTTGCCCGAGCCATTTGGACCGATAATGGTCGTAATTT10.7936507936507936No Hit
GCGCCTATTCCGGCCAAAAGCCGGACCGGTTCAGAAACAATCGGCGCTGA10.7936507936507936No Hit
CCGGTGAGCAGCGTCGAGCTGCTCGAATGGAAAGTGCTGGCGAGCGTCTT10.7936507936507936No Hit
CCGGTCTTGCGATCCTTGCGCACGTCGCTGGCGCCGACGTAGACGCTGAG10.7936507936507936No Hit
GTATATTTACGGCTGATTGTAAAACCTTTTCGCATTCGGTGAATGGAAAC10.7936507936507936No Hit
AGCCAGCCCCAGCTTTTGGTTACAGCGCCTTTTCGAAAATCTTCGAATTG10.7936507936507936No Hit
GAGTGGGTCAACACTTTGAAACGATTGTCTCGCAGATCCAACGTGCGAAG10.7936507936507936No Hit
GTGACGAACTGCAGCAACAGGCGTTCCAGGACCGCCCGGCCGTAGCGGGC10.7936507936507936No Hit
CTGTTAATGATCCTGGTACCACAATGAATCCTTTTTGTTCAAGCCATTCC10.7936507936507936No Hit
CACATTACCTTTCATCTATTAGGCAGAAGGGGTTGAAATTTCTAGAAGAT10.7936507936507936No Hit
CAGCAGCAGGGGTGTGAACTTCGTGCGACACACTCAAGGCGGCATACATC10.7936507936507936No Hit
ACCAGAAAGAGCGTCTGGAAAAAGAGCTTAAGCAACTCAAGACCCAGCAA10.7936507936507936No Hit
CCTTCAATGATCCGCTGGCAGCCAAGGTGCGTTCTACGTTGCGCCGCGAT10.7936507936507936No Hit
CTCGGCTTCCATACACCTGCTGAAATGTTCAGCGAATGCGTTGCATCGAC10.7936507936507936No Hit
ACCAAGTACCATAATCAAGGGGATCGCTCCAAACAGATACCTGAAATCGG10.7936507936507936No Hit
GATAAAACATTACCCGGAGCGGATCATTCATTTGCTCTTAATCCTGAAGA10.7936507936507936No Hit
ATTTAAAGGTGCGTGCGAGTATGTTACGATTGAAATCGAGTGTTTAAGAA10.7936507936507936No Hit
CCATTTACGTTGTGTTTTAAATCTGCTTTCCCTGCATCAATTAAGACATT10.7936507936507936No Hit
CCATTGAACAGTTCTTTAATGACTTGAAAAAATCTGGTCTCTATGACAAT10.7936507936507936No Hit
GTCTACCATTCCAAGACCTGCTCTTAAAATCGGCACAACTCCGAGTTTTT10.7936507936507936No Hit
GAACTGGATCTCGCCGCCGTAGAGGCGGCCCGGATAGGTGGTGCTACCGT10.7936507936507936No Hit
GAAACATGATGTACAGGTCATGATTGAAGGACCTGGTCATGTTCCAATGG10.7936507936507936No Hit
AGAGAATAGGCTTCATCGATGAACAAAATGCCGCCTAGTGATTTTTTAAT10.7936507936507936No Hit
TCCAAATGGCTGTTGCCGCGTCTGCCGTCGTTTCGCGAAGCGCATCCGGA10.7936507936507936No Hit
ACAATAAAATAAATCATGCAGTTTGTGAAGGAGGGAATACACATGCGACT10.7936507936507936No Hit
ATTCTCGCCCCCACCCCGCTACACGCGGCATTGACCGGACCGCGAGGAGC10.7936507936507936No Hit
GATGAAGATAATCGATAAAGTGAATAGAACACTGACGGAACTTAATACAC10.7936507936507936No Hit
GGACAAGGAGCCGACGCCACGAGCTTTCCTGCGGCAGCTGCTGTCTCTTA10.7936507936507936No Hit
AACCAGCGGCGGTTTCAGCGCTGAACTCAAGGGTTTTACCGGGGGCGGGT10.7936507936507936No Hit
GTTTTTCTGCTGCACTTGCCGTCTTGTTTCTTCAGGTGTGGATTGAATTT10.7936507936507936No Hit
GATCTGCGGCAGCTCACCGTTCTTGCCCTTGAGCTCGGCACTTTGCGGAG10.7936507936507936No Hit
TCATGGCCTCGCTCGACGGCTTCACCGCGATGGCGGTCATTGCGCTGGCG10.7936507936507936No Hit
GGGTAACCGTTTTGACGCCTGAGGAATATCCAGCTATTATGACTTATCAG10.7936507936507936No Hit
CCCCCAGCACCACCATGATCCGGTCCACGGCGATGCCGGCGCGGTGCGAC10.7936507936507936No Hit
GGTTACTACATGTGGCGCATGCTCGGCGCCGGCGCCGATAGCGTGATTGG10.7936507936507936No Hit
GCTAGTATTTGCCCCACTGCCTCCCGGAGACTGCCCCCATGACCGAGCCC10.7936507936507936No Hit
TGCAAGAGCTGGAGAAGGGCCATCACTCATTGAAATGAAAACGTATCGCT10.7936507936507936No Hit
GGTGATGGGCATGGCGTATGACGAGTCGCCGCTGTTTTCCGAGATGGAGT10.7936507936507936No Hit
GAGCACTACCTACAGCATCTCCGACCTCGCCCGCGAGCTCGACATCACCA10.7936507936507936No Hit
CTGTCGTTCACCTTCGGCGCGGTCGCGGTGTTGGCGACCCCGGATGGCAC10.7936507936507936No Hit
GATACACTGCCACTGCGGGTAGATTTCACCGTTCTTGCGACGGTTCCAGA10.7936507936507936No Hit
CCTCTGGACCGTCGTGCCCTTGTTGATCCTGATCGCGATGGCCATTCCTG10.7936507936507936No Hit
CCCGTGACCTGGGTGCGCTGCCTGCATCCCGCCTCGCGCTACAGCCTGGG10.7936507936507936No Hit
CTGTACATGTTCCTCAAGATTCAGCTGACAGACCAGCTTTTCATAGGACG10.7936507936507936No Hit
ACATGAAGAGCAAGGACAACTATTTCGTCGGCTTCCCCGCCGCCTGGAAC10.7936507936507936No Hit
GCTGATGTTAGGCACATGACAAAGGAGGAGAAAAGCATGTACCCTCATAC10.7936507936507936No Hit
ATTCAGGCAACGAAGCAAAAGCAAATGAAGTCGTCGATGAGATCAAAGCA10.7936507936507936No Hit
ATGAAGCTCACCAGTGCCATGACGAGCCAGGTCTCGTCCGGCTCCGGGCC10.7936507936507936No Hit
TCCTCTAAAATGCTGAGCGTCTCTGTATCAAGATCATTCGTCGGCTCATC10.7936507936507936No Hit
ATGAAAATCATTATCATTTAAAGTGTACATATAATAATTGAAAATGATTA10.7936507936507936No Hit
TCCGTGTAGCGTCCGGCCCCGGCCAGCAGGCTTTCGTCCTCGAGTCTGCG10.7936507936507936No Hit
TCTCCATACCTCTTTTCCTATTGCATTCGCATTTTCCGATCAATTACTAG10.7936507936507936No Hit
GTCCATGGCTTGGCGGTCTTGACCAGCAGCACCGCTGTCGGGCAATAGCG10.7936507936507936No Hit
CACCACGCCAATGTGCTGCTGCAACCGATACTTCTCCTGCAACGCAATCA10.7936507936507936No Hit
GGTCTTTACCGGCGGTGACGTAGGCCGCCCCTTTTGAGGCGTCCAGGGGC10.7936507936507936No Hit
CTATGCGCTGTCCCTGGCGTGCTTCGGCTTTTGGGTAGCGCTGGCGCGGG10.7936507936507936No Hit
ACCTGGCCCTGGCAGCGCGAAGTATTGAACGCAACCTGGGCCAGGCATGG10.7936507936507936No Hit
CCGCTGCTGCACCGAATCCGTGCAACGCGGCGACTGCGGAGGTGCGCGGC10.7936507936507936No Hit
GTACAAATCTCATGAGACTCATGTCGACAAAGAACATTTTCTTTATTCCA10.7936507936507936No Hit
CGCTTTGTACAGCCCGAGCACGGCCTGCGGATCGATGTTCCACATGGTCA10.7936507936507936No Hit
ATCAAGGTGAGCATCGAGCGCCTGCCGTCGCTCACGGTCCGTATCGACTG10.7936507936507936No Hit
GCCCCACGGTATTCCTTGAGCAACAGCTCTCGGGCGTTCTTGGCAACCTG10.7936507936507936No Hit
AAGTCTTACCGCTGGTTTCCGTGATCATCCCCACGCGAAATGCTCTGCAA10.7936507936507936No Hit
CTGCTGGGCCTGACGATGGTCTCGCCCCAGGGCCTGATCGTCACGCTGCT10.7936507936507936No Hit
TCTTCCTGGTGAGCATAACCAAGAAAATATTCTAGCAGCTATTTGTGTTG10.7936507936507936No Hit
CTTGTCCACTCTTTATATGCTTTTTCAACTGCTTTATCCTGATCATCCAT10.7936507936507936No Hit
CACCTACACGCCGGAACTGTATCCGACCTCGGCGCGGGCGACGGGGTCGG10.7936507936507936No Hit
GAAGTGAAGCAGATTGTCGTCGATGCTGCGGGTGGTTATCGAGATCCTGG10.7936507936507936No Hit
CATCTATTCCACCCATCATGATTATGTGGATTTTCTTCGGTCTTTACATG10.7936507936507936No Hit
TTTGCGGCATGCCGCCTTTCCGTTGTCCGAGAAAGTCTTTTCCTTTCAAT10.7936507936507936No Hit
GGAGAGTGCCACGCCCTCCTACGAGCATATGATGGACAGCATGCGGCGCA10.7936507936507936No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)