FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n01_Tr4_C4I3c_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n01_Tr4_C4I3c_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40
Sequences flagged as poor quality0
Sequence length251
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGTGCCTGAATCTAATCTATTGATTTTAAAGACTATTTAATTTTACT12.5No Hit
AGGTCGGATTGCCCTGGGCGATGTAGCCTGCGCTGCCGCACGCCGGGCAC12.5No Hit
GGCATGCCGCCCGTGACGCTCCGCGTCACAAATGGCACAGGCTGCGGATT12.5No Hit
GCCTACGCCTGCACCTGCTCACGCAAACAACTCGAAGAATATAACGGCGT12.5No Hit
CCGATAGCCTTGTACTGCATACCGCAGTGCAAGGAGGCATCATGAAAAAG12.5No Hit
GTGGTGCACTGCCGCCTGGGTTCGTCGGCGGCATCGGTGAACAGCGGTGG12.5No Hit
TCATTGATGCGCCACAGCTGGAAAACGCGCTGCTGAATCTGTGCATCAAC12.5No Hit
CGCTCGATGTGCGCCAGCGGGAAGGCGTAGGCTTCGCCGCCGACCTCGAC12.5No Hit
GGATTACGGCTATCGCTGCACGCTGGTCGATGATGCCTGCGCGACCCGCG12.5No Hit
CTACGCCACCGGGCAGGTGCTCGCCGGTGAGAACATCCACCAGGAACTGG12.5No Hit
GTGGTGCAGGGCGTGGAGGTGGACGACAGCCACGTGCAGATCACCAAGGG12.5No Hit
GTCCAGCAGGCTCTGGCCGGCCGAGCTCTCGTAGAACTCGGCCATGGCAA12.5No Hit
CTCATGTATCAAGATGTGTAACAAAACCTTAGGATAGGCGCATCGATTTT12.5No Hit
CATCCGCTGGCCGCGCTGGAACGGGTCAATTACCAGCAACTGGCGCGCTA12.5No Hit
GCCCCCATCAGCAGCCCGACCGACGCGCAGGCGTTCATCGACCAGCACAG12.5No Hit
CTCAGCATCGGTGCCTGTCTGGTCACGGTGATGCTGGTCGACAAGATCGG12.5No Hit
GGGAGCCGGCGAGGAGTTTCTTGTCCCCGGTGACTGTGTCGAGGCGGTCG12.5No Hit
CCCCGGCCCCGACTGCGACGTAGAGTCAGTGACCATGTCCGAAGTCACCG12.5No Hit
CACATGAAAACAGTACTCAGCGCCCGCAACCCTGGCTGGGCGGATCAAGC12.5No Hit
AGCAGTGGAGAACAGTCAGGCAGTAGCGGATCTGCACCGGGACCGCGAAC12.5No Hit
CTCCTCATCCGTCATCTTCATCAGATGATCACACGTATTTTCAAGCATCC12.5No Hit
GTATGGACAAGGATCTGGCGATGGAACTGGCGGTGCGCGGCGTAGTTACC12.5No Hit
GGCCCTTGACACTGATCAGCTCGGGCTGGACGTTGAGCTCAACACCCTTC12.5No Hit
TCCAACTGGATCCCGAACGCTGGTTAATTTCTAGGGGTTTAGAAGGACGT12.5No Hit
GTACTTTGTATTCCTTGGTTGGAACCCACTGACGAATCTGGTCAGCGAAC12.5No Hit
TGACACTGCGACTTGGCGATATCGCCCCCGACTTCGAACAGGACTCCAGC12.5No Hit
GTGCAACCCGCAGCAACGTGGGTCGCAGCATGGCGGTGATCTTCATCGAG12.5No Hit
ATGTAGATCAGGTTCGGCGTACCGCCCGTGAGGCCGATATGCGCCTCCTT12.5No Hit
GCCTTTTCCTCCAGGGAAACAAGACCTGCGGAGATCGCGGAGGTAGCAGA12.5No Hit
GTGTGGGCGAGTGCCATCAGGATGAAATCCAGGCCCAGCCCCAGGCACGA12.5No Hit
GCCTCGCTGGGCACTGGTCGATCTTCGGCGTCGGTCAATCCCTTGAGGTA12.5No Hit
CGAGGTATTCGAGCAGAAGCACGACAGCGCCTGGCAATCATTAAGCATTG12.5No Hit
GATCCACTCCTGGTGGGTGCCAGCGTTTGCGGTCAAGCGTGACGCGGTAC12.5No Hit
GGTAGAAAGTGCACTAGACATGACAGACCGTCCGCACCGACCACAGCACT12.5No Hit
CAACAGCTCGTCCGGTGCGTGCCGTTCGATGAAGGCGGCAACGCCCTGCG12.5No Hit
GTCATGAACATTGATCGTGGCGAGCACAGCTTCGCAGTGGTGGTGAAGGA12.5No Hit
GGTTTTTGACCTCGTCATGCATTACGGTTTCGCCACCTGGCGTGCGCCCC12.5No Hit
GCATCGGTGTGCCTCATCACGTGGTCAAGTCACCAGTGTTGGGGCCGCGT12.5No Hit
ATATATGGGAGGATCGCAAATGTCATGTATCCCTGCGCCCTGTCTCTTAT12.5No Hit
GTGCTGACGCTGCAGGGCTACCGCACGCTCACCACTTATGGCGCCTCCGA12.5No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)