FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n01_Tr4_C4I2c_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n01_Tr4_C4I2c_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95
Sequences flagged as poor quality0
Sequence length251
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTGGGCAGCCCGGTGGTGCCCGAGGTGTAGATCACGTAGGCGAGATG11.0526315789473684No Hit
TTTTTTATGTTGAATCAGGTGCTTGCTTTGCATGAGGTAGCTGAGCTGAA11.0526315789473684No Hit
GCTAGATTCCGTTCTCAGCGATCACGTTCGTTCCGTGTTCATTGAAGTCT11.0526315789473684No Hit
GGTAAAGATCGCCGGTACCGGACTGCGATCGAGAGATGCCCTGAAATGAC11.0526315789473684No Hit
GTTGTGGAACGGCGGCATCGGCACGTACGTGAAGTCCAGCGAAGAGTCCC11.0526315789473684No Hit
GGTCAACGCGATAGCACCGGGCGAGATCGACACGTCCATCCTGTCGCCGG11.0526315789473684No Hit
CTTACAACCCCAACGCCAAACTGATGGCCGAAATGCTTCAGTCCGACTGG11.0526315789473684No Hit
GGTAGTGTTCTATCTCGCGAAAAATCCACGGCCGCCCCTGGGCTGCCCTG11.0526315789473684No Hit
CTCCCAAGGCCGAGCCGGGCTGATCCGCCTGCGGTCCGCCCCACTCGTCA11.0526315789473684No Hit
GGGAAATAGGGGTGGCTGAAGGTGAACGCCAGCAGGCAGGGTTGATGCCC11.0526315789473684No Hit
TTCCAGAGCGTTCCGCTACTCTCAATCCAACTTAAGGGCTGGTCCCCGTT11.0526315789473684No Hit
ATGTCCGAATGCGGTCTCGCTTATTTATCGATGGGGTAGGCCTTTGCAGC11.0526315789473684No Hit
ACTCAACTCGAACGCTACGAGCTGGAAGTCAGCGAATCCAGTGCCATCAT11.0526315789473684No Hit
GTCCAGCGCACCGGCCAGCAACGCCTCGCTCATGGCCGTGGCGCCGGAGA11.0526315789473684No Hit
CAATGGAGCACGCTTGCGGTAATGAGGTGGAATCGTCGCAGGCCCTCTTG11.0526315789473684No Hit
CAGGAATAGCGGGTTGCAGCCCACCAGCAAGGTCAATCCGCTGGCCCAGC11.0526315789473684No Hit
GGCGAGCTGTTGCGCAAACAGCGCGAGGTACCTCCTGCCGAAGCAGTGGC11.0526315789473684No Hit
CCACAGAAAATTCCCATCCATCGTTCAAGCCGCCCAAGGGCTTGGCCTCG11.0526315789473684No Hit
CAAGATGGTCGACACTCAGGGTAACCCGGTCAAACTGGAGTTCCTGATCG11.0526315789473684No Hit
ATGCATAGAGCTTGGACAGCTCGTGCAGGATCTCCGGCACATTCCAGATC11.0526315789473684No Hit
ATTGGACGCTGGCTGGTCGAGCAGGGCCAGTTGAAGAAGGAGGAGGTCAC11.0526315789473684No Hit
GCATCGGACACTCGCCGCGCAGATGGCCGCGCAGGGCGGGGAGGAGATTT11.0526315789473684No Hit
GCAACAGGCCGTTCTTGAAGCTGTTGTTGAAGAAGATGTCGGCATAGCTC11.0526315789473684No Hit
GTCTGTACATCTGACACACCGCTTGCTTCCCGCAATCACCGCCTCTTATC11.0526315789473684No Hit
TAGCTGACCGGACCTGGAACAGGCCGGGGATGGACCTGTGTCAGAGGCCG11.0526315789473684No Hit
AGGCAACACCAAGGACTCGCTGGTCAAGCGCGGCTTCGGCACCAATGACG11.0526315789473684No Hit
AGTCGTACAGCGGCTCGACCTTGGGACCGGTATGTTCTTCGATGGCAAAC11.0526315789473684No Hit
CGCTGATATCGACGCGTTCGTCGCCGCTGTTGCGGCCGATACGCACAATC11.0526315789473684No Hit
ATCATGCGTTGGTAAAAGCGCAGTCCCGGCGTGCAGCTGCGGTCGGCTGA11.0526315789473684No Hit
AAGCTGACCCAGATGAACTTCGAGACCAAGGCCGAGGCTCAGGCCGAAAA11.0526315789473684No Hit
GGCGTTCTGTGCGGGCGGCGATATTCGCTCGCTGTACGAGAGCCACCGCA11.0526315789473684No Hit
GACGTGCTGGCCGGCGTGCAGGCCCTGCCAATGCCCATCGGGCTGGAACA11.0526315789473684No Hit
CCATCACCTATATCGCCTCGAGCGAGGGCGGCGTGGAGATCGAACAGGTC11.0526315789473684No Hit
TTCTTACTCTATGTTTCATATGATAAGTTGCTATTTTATAAAATTCAATC11.0526315789473684No Hit
GCTCAAGGGCCCGGGCCCGTTCACCGTGTTCGCGCCCACCAATGCCGCAT11.0526315789473684No Hit
CCATGGGAGCCTTCAGTACACGAGTAATCGATTCAAAGGCGGCATGGTGC11.0526315789473684No Hit
GACCAAGCGCTGGTGATCGTAGCCGTAGCTGGCGAGCTGTACGCCACCGG11.0526315789473684No Hit
GGATACCGAGGCCAACAACGGCGTGCTGATTTATCTGCTGCTGGCCGACC11.0526315789473684No Hit
CATCAGGGCGAGAGCGTCCATTGGTCGACCAGCAGGCGCACCTTGGCGTC11.0526315789473684No Hit
CAACTGGTCAACTGGTTCTCGGTGGCCTGCGAGTTGCAGATTGACTGGCG11.0526315789473684No Hit
GCGAAAAGCTGCGCCTGGGGCCGCAGCACCATGTGGTGGAAATCGGCACC11.0526315789473684No Hit
ATGTAGGTGACGTTGGCGCCGTGCCAGAGACCGCCCAGCAGCATGGTCAG11.0526315789473684No Hit
CCGCCTGGTGGCCGACGACGCACCCGAGTTCACCCAGATCGCGCCCGACG11.0526315789473684No Hit
GGCTGAAATGGCCAAGGTCGGTGGCGTCGGCGCGCGCAAGCTGGAGCGCT11.0526315789473684No Hit
ATTAAGAGCTAATTTTACTATCAAGAGACGCTTAGTGCTATCTGTAAATA11.0526315789473684No Hit
GTTCCAGGTGGCACTCAGTGGCAATCATCAGAACATCCCTTTCCTGTTTG11.0526315789473684No Hit
TCTCTAAAGTTAGCTTGACGGGTTGCCTCAAAAAGTAAAAGTACGGACTT11.0526315789473684No Hit
AAATCGAGTAGGACGGGGCACGAGAAACCTTGTCTGAATATGGGGGGACC11.0526315789473684No Hit
GGTATGGCGCGAGGGCGATGCCGAACGCGTGGTGGTCGGGTACGACGGCG11.0526315789473684No Hit
CGCTGAGCCAGACGCCCCGCAGCCCAGCCGCCGACAAACAGCGAGATGAC11.0526315789473684No Hit
CGCTCGAGCACTATCAGCAGACCAAAAACCTGCTGGTGAGCTATGACACC11.0526315789473684No Hit
CAACTACATCGCGCGTCGACTCGACGAGTACTTCCCGGTTCTCTACACCG11.0526315789473684No Hit
AGCCCGAGGCACACCATGACCGTCAGCGAAGACAAGTTCACCCGGCAGAC11.0526315789473684No Hit
ATTGATGATCGTGTCGGCGAAAATCTTGTTGTTGCCGACCAGGTTAAGCA11.0526315789473684No Hit
CTCGTAATACGCATCGCGGTCGGACTGATACAGCACCGGCCCGTTAGCGC11.0526315789473684No Hit
ATATATAGCGATGTTCCACCTTTCTGAAAGCGATGTTTCAAGCTCGGTTC11.0526315789473684No Hit
GTCAGACGACCGTGCGTCTTGCAAAGTTGCTGCGTGCCTATGAACAGACC11.0526315789473684No Hit
TTCTGAACAGGCGACAATAATCTGCCACTTGCGACACTTAAAGCGCCCAT11.0526315789473684No Hit
GGAAACGGTCTGGGGCGGCTACGTGGACATCTCGGCCAACCGGGCGCCGC11.0526315789473684No Hit
CGCCCTGCAGCAGTTGCTGGCTGTCATCGGCCTGCACGCCCATGTCCCGC11.0526315789473684No Hit
TTCCACACCTTGTCGCGTGCCCGTGAGCTGGGCAAGACCGTCATTCTGAA11.0526315789473684No Hit
GCCATGACTTCATCCAGCGAATACAGGGTCAGGTTCACGCGGTGATCCGT11.0526315789473684No Hit
CTCAACGTCACCGGCTATCACCCGGACCTGATCCGGGTCGGCCTGCAGCT11.0526315789473684No Hit
CGGTGAGGAATTCACCTTCGGAATCCAGGTGATGCACACCGGCCACATTC11.0526315789473684No Hit
ACACTCATCATGTCCGAAGCGTAAGGGTCGGAATTCCTGTCCGGGTGCAG11.0526315789473684No Hit
GACCTTTTCCACCAGTTCCGGCGCCACGGTCGTGACGCCACGCAATGCAC11.0526315789473684No Hit
TCGCCAATCCATACCGGACCTTCGGCCCAGCGCATGCCGGTCGCGAGCTT11.0526315789473684No Hit
ACGGTGGGGCTGACCGAGTTCACGCGCATGCCACGCGGCAATTCGATGGC11.0526315789473684No Hit
GGCTTATACCGGCGCAGGGCAGGCGTTCCTGCTGGAGCATTACCTGACCC11.0526315789473684No Hit
TCCTTGCAGGCACATCGCGCCCCGCCACAAAACCACCGATGCATCACTCC11.0526315789473684No Hit
CAATACCGGTGGCGCCGGCGCAAAACCGGCCAAACGCTGCGCCGGAGCGC11.0526315789473684No Hit
GTGTATTGGGCAAAGGCTGGGTGCTGCCGTGGGAGCAGAGCCTGCGCCGT11.0526315789473684No Hit
GCAGTGGTGTGAGCAATTTCTTCAGCAACCCGGGCGACGTGCCCAACCTG11.0526315789473684No Hit
CAACTGGATCATCAGCAGGGTCAACACGACCACGATGAACAAGGGAACGC11.0526315789473684No Hit
TCGGTAGACAGTGTTGTGGTCGAAGATGATTCGATCCGGCAAGCGCGGGC11.0526315789473684No Hit
GCCGCTGGGCCATGCGCGAACGCGCCCGTCTTTACCGGTTCATCGAACTG11.0526315789473684No Hit
GTACGCAGGTTGGTCACCAGGGTTTCCATCTTGGCCTTGGAATCGCCCGG11.0526315789473684No Hit
CACTTTACAGCGTGGCGTTTCGATGATTGGCTGGCGAAGGCAATGACTTT11.0526315789473684No Hit
ATAAAGAACAGCACGCTGAGCTGCACACTGCCATTTTGCTTGTCCTCCAG11.0526315789473684No Hit
AGGTGGTCTGGGTGCCGCCGGACAGCGACAGCGGCAGATCGAAGGTTGCA11.0526315789473684No Hit
ACACGCACCCTCGAAGTCGGACGCACCTCGATCAAGATGATGGTCGAAGT11.0526315789473684No Hit
CTGGCGAGCTGGAAGCGATAAGTGGTGGCCAGCAGGCCATGCTCTTCCAG11.0526315789473684No Hit
CGTCTGGCCGATCTGGAAAGCGACATCGACAAGCTCGAGCGCGAATTCTC11.0526315789473684No Hit
TTTGAGCTTGGACACGCGCCCTTCTCCCTGGCTTGCTTCTGTCAGCGTTT11.0526315789473684No Hit
TGGCAGAACGAAGCCATGTTCGGCAGCTCTGCTGACGTGTTCATGGATAA11.0526315789473684No Hit
GTACCGGCACTACCTGACGCGCAGCTGGCAGCACCTGCTGCCGCACGTCG11.0526315789473684No Hit
GATTCCGGTCGTGAGGAGGGGATATCCATCTCTGCGAATGACCAGGATCC11.0526315789473684No Hit
GGCCAGATCATCGATCACCCGGCTGACCTTGCCGTCGGCGGGATCGATGC11.0526315789473684No Hit
CGCTGGCACTGACCAAAGACCTGATTTGAGCCCCTTGCCTCTGTGCACAG11.0526315789473684No Hit
GACACACCGTTCCGCAGGAAGCCCGATGGGCGGGGTCACCCGCAGTGCCC11.0526315789473684No Hit
ATGTGGTGGGCACCGCGGTGGATAGCGGCGGCAACTTCATCGCCAGCGCA11.0526315789473684No Hit
TGCAAGAACACGCCTGCCACGTACTGATTCTCTTACGCTTGCATAAGCTC11.0526315789473684No Hit
CATCAAGGGTGACGCTCATCGCGCCCAATGCCGAAACTTCACCACCGTTG11.0526315789473684No Hit
CAATGCAGCCCGCACGACGCGGCTCGCGGCCCAGCCAGATGTTTTCGGCG11.0526315789473684No Hit
CCATTCCAGGCGCAGGCCCAGGTTGCCGGTGGTGGTGGCCACATCCATGT11.0526315789473684No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)