FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n01_Tr4_C4I1b_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n01_Tr4_C4I1b_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35
Sequences flagged as poor quality0
Sequence length251
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCTTCCCACTGCTCCCAGTGCAGGATGATGCCGAAATGCGGAACCGGC12.857142857142857No Hit
TGCCCGCATCGGTGCGGGCAGGCCTTCGTCGGTTCTGTTCGGATCGCTCG12.857142857142857No Hit
TCCGGATGCTCGGCATTCCAGTCGGAGATGATGTTTTCGAGCGCCTCGTT12.857142857142857No Hit
TAGTATGCGTTGCCATCAGGCGTGGCACTGACCTGGCGCGTGGTGCGCTG12.857142857142857No Hit
GTCTTGACCGATCTGGTCACCGAGCTGCAGGTGCTGGGCACGCGCGCCAG12.857142857142857No Hit
CGATCATTCTGGTGGTGTGGGCGCGCTATCTGGTGCACACGCTGCTGATG12.857142857142857No Hit
CACGTATTGCGGCTCATCACCGCGGGCAAGCAGTTCGTTGATACGTGTCA12.857142857142857No Hit
CGGCCAGGTCGTCCTGCTCGCACAGGCACACCGACAGGCCGCGCCCGGCC12.857142857142857No Hit
TCAATGCACAGGGCGATTCGGACTGGCCGGGCAACCCTTGGTTCCCCGGT12.857142857142857No Hit
GATTTTCTTGAGGAAGTCCAGCCACGCCTGATGCCGGTGTTGGCCTGTCT12.857142857142857No Hit
CTGCTATTGACTATTCTGCCCGCCAGCCTGATGGCGGCGGTGCTGGGTGG12.857142857142857No Hit
GGTAGCGGTCAAGCGGCTGCAGCGTGACTTCACAGGCGAACTGCGGGTTC12.857142857142857No Hit
CGGTGGTGACCTTCAGGAGTACTCACGTGACACATACTTATATTCAGGGT12.857142857142857No Hit
CTTCTGCACACGCTCGACGAAGTAGAAATAGCTGTCGGCGTTGAAGAAAT12.857142857142857No Hit
ATTTCATGCGCATACCCCGAATGGTGGAAAGAACCGGCCGGCATTGCGCC12.857142857142857No Hit
GTTCTGCTCGCTGGCCATTACCGGCGGCTATTTTGAAGCCGTCCACGTGC12.857142857142857No Hit
TCCTGGCGCTGATAATACCGACGGTTTCCGCGGCGTTTTACGGGGTTGAG12.857142857142857No Hit
TTGTTGCGACGGCCCATGGACAGGATCTGGGTGAAGCCGCAGTAGAAGAA12.857142857142857No Hit
CCACGTCTTCTCCCGCGGCCACCATGTCCACCACGCTGCGGGTGCTCTGC12.857142857142857No Hit
CACCAAAATATAACAGTACCGATTGGTATCGTTATGTCGAATCGTTGTCC12.857142857142857No Hit
GGTTGAACAGGTCCGCCGTCAGGAAGGCGCCATACAGGCCCATCAGCTGA12.857142857142857No Hit
CTGTTGGTTCGAGCAAACCGTGTTACTGCATCTTCGACATCGCGCTGTCG12.857142857142857No Hit
GTCTATCAGTTCGACTACGTCGACGATCCGGCGAACGTGATCATCGACCT12.857142857142857No Hit
GTTATGCACCGGTCCTGACAACCTGTCGCACGTTATCCAGGGAACGCTGG12.857142857142857No Hit
TATTCCTCCCGGACTGCACGCCGGAAGAGATCAGCTTACGCCTTGTCGAC12.857142857142857No Hit
GCATGTAGAACGCATTCTTGTCGTCCAGATGCTTGGCGGTGAAGGTGATC12.857142857142857No Hit
CCTCCAAACCATCAAGGCTCTGGAATACGTCCTTGCGCACCATCAGGCAA12.857142857142857No Hit
ATCCGACTCTCGTCCCCAGCGTTCGTCGCATCACGCCCGCCAGTGCTGCT12.857142857142857No Hit
CCCTGTACCCGGAAAAACTGACGACCCGCGACAGCAACCCGCTGCCGTTG12.857142857142857No Hit
ATCCGGGTTCGCACCTGGACTTCCATCTACCTTCGGGTCTTCGGCGTCAG12.857142857142857No Hit
AGGCACCACCATGCCAGTATCTGTCGAGCATGACATCGCCAACCACCAGG12.857142857142857No Hit
CTATATATCGATATAACCCGATATACCGATTCTTGATGAGCTAAGTCCAA12.857142857142857No Hit
GTAGCGGGGCTCAAGTACACCACCGAAGCTGCGGCATTCACGCAATAGCC12.857142857142857No Hit
TCGGTCCTCGGTGATGCTGCGCTGGCGCTCGCTGAGGGCGATGCTCATCA12.857142857142857No Hit
TCGGTGCCATGCTCGCATTTCGGAATGGCGTCGCAACCGGTACCGAAGTG12.857142857142857No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)