FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n01_Tr4_C3I4c_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n01_Tr4_C3I4c_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41
Sequences flagged as poor quality0
Sequence length251
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCCGTCCCGAGAGTGAATCGCATCCTTCGTGATGCAGTACAATCGCGG12.4390243902439024No Hit
CATCCAGCGTGCCGTAGACCATGGCCGGCGAGCCACTGGCATAGACGTGC12.4390243902439024No Hit
ATCAGATCCAGCGACAGATCGTGGGAGGTTTCCGGGGAAAGTTTGAACAA12.4390243902439024No Hit
CATCTCACCATCTTTCGAAGTCCATACGACTTGGGCTTTCGAGGGGAAAT12.4390243902439024No Hit
GAGTAGTGGCTTGGGGTGACGCACACAAAGGTGGCACCGGCGAACAAAAA12.4390243902439024No Hit
CTTCTTTATAGCGCCAAAACATTTCTTGCTGCACTTGTACACGTTCGACT12.4390243902439024No Hit
ATACTACGCCTCTCTCCGGGTATCGGCGAGGGCAGCTCCTGGATTGCAGA12.4390243902439024No Hit
GGGGGTCACCGAGGTACCTTTTTACCTGCAAAAGGAGATGCCCTGATGGG12.4390243902439024No Hit
TTTATCCAGCGTCTGTTGGCCAAACCCCGGTATGATCGGCCCCGCGCTGG12.4390243902439024No Hit
CCTGTAATGAGACGGCTATACAAAACAGCTGGTACTCCATATTGTACCGT12.4390243902439024No Hit
GGTCGTCCATGTGGGAGTCGCAGCCCTGCTGGGTCACCAGGATGTCGGGG12.4390243902439024No Hit
CTGTGGCAGCGCGACAAAAAGTCCCGCCTTGGTGACACCGCCTACGCGAC12.4390243902439024No Hit
GGATATTACATCAAGTAATATTTTCAGAGACTTTATTCATCAGCTCAAGC12.4390243902439024No Hit
GGTGGTGCGGCACTTCCAGGACTTCTCGGACGAAGTCATCAGCTCGCCGG12.4390243902439024No Hit
CTGTGGCTGCTCGGTCGCGCGGCGGACGGTTCGATGCGTGACGCCATGAG12.4390243902439024No Hit
CAATGAGCCTTTCCCTTCGCGACCAGTTGCTCAAAGCAGGGCTGGTCAAC12.4390243902439024No Hit
GTATTTATCTGGTGGATGACTTGCCGTCCGAACTGGACGAGAGCCACCGT12.4390243902439024No Hit
AGCCAACTCAGCCAGGGTTTGAGCAAAGCCAAACTTTTGGTACTCGACCA12.4390243902439024No Hit
CCTCTGGGAACCTGCTGTTACAATGCCGCCCAGCGAATCTCGGACTTTTC12.4390243902439024No Hit
TCGTTGTCTAGATGCAGCTCGTAAGTTCTTGTATTGGACGCATTTAAAAT12.4390243902439024No Hit
CGCCCACTGGGCGATCTGGCCGAGCGCACTCCCCTGGCTCGTCGCGCACC12.4390243902439024No Hit
TGCTTCGTCTTGTCATCATCTATGAAGGCGACTGGCTCAATATGTACATC12.4390243902439024No Hit
GGTTTAGAGAGCCGACGCAAATTGCTTGGGTGTTATATGGTCAAGCCTCA12.4390243902439024No Hit
GGGCATCCCTTTTGCTTGCGGCTTTTTTGTTTGAAATCAGAAAAACATGT12.4390243902439024No Hit
GCCTTCTTCAGTGGCCGAACCGATCAGGCCGCCGGCCACGGCGCCGATAA12.4390243902439024No Hit
CCATTTATCATAGTCAGACTGCTTCATCGCATTGACTTTGAATTTTTGCT12.4390243902439024No Hit
AGCATGTATTTACCTCATTTTCCTACCGGCCAAAAGATTACCTTGAAAAA12.4390243902439024No Hit
CAACACAACGTAGATCAGCAGATAACGACCGGTGCGTTTCAGCGCGTAGC12.4390243902439024No Hit
GTGTACGAAGAAGTGCCAGGCTGGACCGAATCGACCGTGGGTGCCAAAAC12.4390243902439024No Hit
GGCCTGGCACGGAACGCCGGCAACCGACAGGAAGCGGTTCTTGTTCACTG12.4390243902439024No Hit
GCGTGAGAAATACATGGCTCAGTGCACCCAGGCGGCCACTCCGCAAATAG12.4390243902439024No Hit
GACCTGCGGCGATACATCGCAGTACCCGCCTCGCTCATCGTTCGATGAGC12.4390243902439024No Hit
GACTATATGAGCACCGTTTTTTGAATAATGGATAGTACCTCTTGTAGTCT12.4390243902439024No Hit
CCACCAACTCGGCGCTGGTCAAGCTGCCGAGTGCGGCGAAGAACCTGATC12.4390243902439024No Hit
TGTCTGAAGTCCGTCATTCGCGAGTGATCATCCTCGGTTCCGGCCCTGCC12.4390243902439024No Hit
TATCTGATCCTGCTCGGCACGGTGATCCCGCCGATTGGCGGGGGGATCAT12.4390243902439024No Hit
CCACCAAACCGATTTTTTCCCCCGGTCGGATGCTCAGGCTCAAGCCGTCG12.4390243902439024No Hit
TTTGCGTTTATTTGTGCTCGCGCATCACGTGCCACACGTCCTTCATCTTG12.4390243902439024No Hit
GACCTGGCAACCGCGCAAACTGGGGCAATAACGCATGACCGCTCCACGCC12.4390243902439024No Hit
ATCCTGCTGTTTCGCAACGACACTTCGGCGTTCGACGGCAAGCGCATCGA12.4390243902439024No Hit
GAACGTCCAGAGCGCGCATGGCTTCTGGCTTCAGGTCCGAGCTGTCGTAT12.4390243902439024No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)