FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n01_Tr4_C3I1b_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n01_Tr4_C3I1b_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41
Sequences flagged as poor quality0
Sequence length251
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCACTCAATGCGCCAGTTCTCTTCACCATCGCTGGCGATTTGCGGCA12.4390243902439024No Hit
ACCTGCAATACACCCGCAACGACATGGATTTCGCCCGTGCGACCTTCCGT12.4390243902439024No Hit
CTGCCATATCCAGCGCCTGACGCACTTCGAGCTGGTCGAACGGCTTGTGA12.4390243902439024No Hit
CTTCAACACCGGCCTCTTTCAGCGCTCGAGCAAAACCGGCCTGACGCATT12.4390243902439024No Hit
TATTTGCTGTGAATGATGATAGCAAAAAGACCCTAAGCGGGTGCCTAGGG12.4390243902439024No Hit
CCATACCTGCTGGGCCTGCCGCGTTACGCCGACGCGAGCAACATCAATCT12.4390243902439024No Hit
ATATAACGCGGCGCCAGGTACTCTTTCAGGTAACGCAGATAGCGCTCGCG12.4390243902439024No Hit
TGTATGAATTGTTCCGCCAGATCAGGATCATTGGCCACTTCCTCCCAAAA12.4390243902439024No Hit
GACTTCGTCAAGGCCATCAAGGCTACTGGCCGCAAGCAACTGATCATCGC12.4390243902439024No Hit
GCTCCACCTATCGACTAGAAAGAAGCCATTATGAAGAAGCCAACCCCGAA12.4390243902439024No Hit
GCATGAGCCGGCACTGAGCGGGGTCGACACCGAACGCCTGACCCTCAATG12.4390243902439024No Hit
GAAGAACGACGTGATTTTCAAAAATGCTGGTGCGTGCTTGATTGATATCG12.4390243902439024No Hit
CCCAACAACTCGCGCGCGCGTTCGCGGCTGTGCTGCAAGCCCTCGGCCTG12.4390243902439024No Hit
TTCATGCACAGTGCGCGGGCGATCGCCACACGCTGCTGCTGACCGCCGGA12.4390243902439024No Hit
ACCGCGGCGCTTGGCGTGATGATCGCAGCCCTGTTCGTCGCCCCCATCGG12.4390243902439024No Hit
ATGCGTAAGCATGCGCGTTAAATCCGAAATTTTCGTCTCCGCTCTTGTCT12.4390243902439024No Hit
GCTTGAGACGCATGAATATTGGCACGCAATAAAAAAGGAAGCATTGGCTT12.4390243902439024No Hit
GCCCAGCTCGCTCATCGCGAACAGCACGCGACGGTGCACGGGCTTCAAGC12.4390243902439024No Hit
GACAAGACCGTCAAGGCGAGTGTCGAGGCGGAACGTGCGGCGCTTGGATT12.4390243902439024No Hit
GCGCAGCACTGGCAGCAGACTTGAAAAGGATGAAACCCGACAGCCTCCTG12.4390243902439024No Hit
AACCAGGTGCGTAAATTCGACGCACGTCTGATGACGCTGGATGCGCCGAC12.4390243902439024No Hit
CTCATGCCTTTGTCGAACGACCTGCGTAGTTCTGGTCAATCCACTGTTGG12.4390243902439024No Hit
TCTTATTAGCCTATATATTGTTCATCAACTATTTCCCGCTCCCATACTCT12.4390243902439024No Hit
CAGAAACATATTGTCCATAGTAGACTAAAAATCTAAGTGATTTGGACGGT12.4390243902439024No Hit
TCCACACCCAGCGACACGACGATGATGTCGGCGCCGTAGTTGTCGATCTC12.4390243902439024No Hit
GGGGTGGACATCAGTTTTTCACTCGGTAAGGTACAGGCATGCAAGGCTCG12.4390243902439024No Hit
GATTACGCCGCGCTGGCGTCAGGATGCCGAGGCGTTGCTGGATTACATGG12.4390243902439024No Hit
GGTTTAACCTATATCCAAACGTTAAAAATGATTATTATGCCGCAGGCTTT12.4390243902439024No Hit
GTACGTAGAGAAGTTCACCAACGGCGCAGTGACCGGCAAAACCGGTTCCC12.4390243902439024No Hit
CGACGGTGGCACCGGTGCTTCGCCGCTGACCTCGATCAAATACGCTGGCG12.4390243902439024No Hit
TGATCGACGAGACCACCGGGCAGACCGTGGTGCAACTGGTGTAGATGAAA12.4390243902439024No Hit
GACCAATGGCGGAGGTTACGCCGAATACTGCACGGTCCCGGCCGGCCAGA12.4390243902439024No Hit
ACACGACCCACTACAGTTACGACAACCGGGGACGACTGCTGGAAATCAGT12.4390243902439024No Hit
CCCATAATAGGGTTACAATCGAGCATTTTTGTACAGTGGGTGGAAGGGTT12.4390243902439024No Hit
GGGCCTGCTGTTCGGCGCGCTCGAGGGCGGCCTGCAGGCCTTGTTGCGAC12.4390243902439024No Hit
GTATAGAAGTGGTAATATAGCAAAGGTACGTCTTGAGTTCGCTCAAGATC12.4390243902439024No Hit
GTCAGTAGGGGCGAGCGTGCATCTGCATTCTGGGTTGATGTTCAAGCGCA12.4390243902439024No Hit
ACGCTGTAATTGGCGTAGTGCCCGGCGATGCGCCATAGGTGGCGCCACTG12.4390243902439024No Hit
GCATTGATCCAGCCCATGGCCTCTGACGATGTGGCCGCTGCACTCACCGA12.4390243902439024No Hit
CGTCTGGCCAAGGGCGAGAAATTCGAAGCGCTGGCCAAGGAGTTCTCGCA12.4390243902439024No Hit
CTCTGGTACGACAGCGATGCGCTGCTGGCTTTCGAGATGAACCGCGTCAT12.4390243902439024No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)