FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n01_Tr4_C2I3c_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n01_Tr4_C2I3c_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33
Sequences flagged as poor quality0
Sequence length251
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTGGATTTGTTTTCGGGCGTACATCACTTTTTCATACATTTTTACAG13.0303030303030303No Hit
CTTTGAAACTCCGATTTTTGGTAGCCACCAAACGAATGCCTGTCTTCTCG13.0303030303030303No Hit
CACCAGTTTCAAGCCCGGCTGCGGCGTCTGCCTCCCGCCCAGGGCTACCG13.0303030303030303No Hit
TTGCAGGAACGGGCGATGAAGGGCAGGGACTATCGCATCATCGGCCTTTC13.0303030303030303No Hit
GAAGAAACCCACCCATAATGAAATCCGAGCTCCATGCACGACTCTAGAAA13.0303030303030303No Hit
CATAGGATGTCATCAGCGATGCACACTCTTCAAGTAATTTAGATAAATTG13.0303030303030303No Hit
GTTTTACGACGGCCGCTCACCGGAGTGAAAATTCTCAAAATACCTGTCTC13.0303030303030303No Hit
ATACTGTTTGTTGAGATAATACTTGTTCGCTTCCACATACGCTTCTTGTT13.0303030303030303No Hit
ATTGGTCGCCGCCGGTGATGAAATGCCGGTCGATCTTCAGGTAATCCAGC13.0303030303030303No Hit
ATCCACCCGATATCGGTTGAGTTTAGCTCAAATCGAAGTTGGATGGCTTT13.0303030303030303No Hit
CTCATTAACTCATCGACGACTTTAACTCAGTCAGTAGCATCAATGGATCG13.0303030303030303No Hit
CACGTGGAAGGCGACGCCAGCAGGCTCACGCCGTTGCGCTCGCGATCGAT13.0303030303030303No Hit
CTATGTGACTACCAACAGTTTGACCTTTGATCAGCTAAAGGCCCTGTCTG13.0303030303030303No Hit
ATGTAAGGCAGGCCCTCGCCCATATGGCCGAGAATGATGTTGAGGCGCGG13.0303030303030303No Hit
CTTGAAAAGCTTGCCGAAAAACCCGCGCTTCGGTTTCCGAAGCTGTACTC13.0303030303030303No Hit
TCCCCTTGGGACACGATGAAGTGGGCGATCCAGTTACAGCAGGCCCATTG13.0303030303030303No Hit
CCCGGCGGCACCATCCTGCTGGCTGCACATCGCGCCATCGAATCCATCAC13.0303030303030303No Hit
GGGAATGCTCGTAAATATCCATGCTCTCTTCATAGAGATAATTGGAGCGG13.0303030303030303No Hit
GCCCATTATCTGGGCATCAAAGATGGAGAGAAAAATGCGTCAGGTTGCAA13.0303030303030303No Hit
GACATGCTGGAAGCGCTCGGCATGGAAAGGGTGGAGACGACGACGCCCTG13.0303030303030303No Hit
ATCGTTGGAGCCGACCGAGAGGAAGTCGACCATCCGCGCCAGTTCCTTGG13.0303030303030303No Hit
GGTCTTGCAGCGCTGGGCCGACCTGATCGAACAGGAAGCCGAGACGCTGG13.0303030303030303No Hit
CAGCGACGCTGCTGGTGCGCGAGGCTTGCTGGTCGGAGTTGTACATCGAC13.0303030303030303No Hit
AGGCAGATCTTGTGTTTATGCTTCCTTTGGAAGCTAAGATGGACCCTTAT13.0303030303030303No Hit
CTTCGTCGGCATGACGACGGCTTATGACCCTGACCTCATGCTTGCCATGC13.0303030303030303No Hit
TTGGCAACCATGGGCTCCGGTCTGTGGGGCATGTATTCCGGCTTCGAGTT13.0303030303030303No Hit
GATCAGTATTTAGCGTTACATGCCGATCAGCACGACTCTTTTCCTTCCTG13.0303030303030303No Hit
CGCCGAAGGGATTGAGAACCACGACCTGACGGCCGAGCGACTGCCGATAT13.0303030303030303No Hit
GCAGGTACTGGCGTCGGCGGAGGCCTTTGCCGAGGCGAGCAAGCCCGTCG13.0303030303030303No Hit
CCCATGATCAAGACTCCCAAAACCAATATGCGTTGGAGTCGAGTCTATTC13.0303030303030303No Hit
GATTCAGCAAATCGAGCAGTTTGTTGATCAAGGCTATCAGCGTGTGAAAG13.0303030303030303No Hit
CTACCGAACTGGCCTTGGTATAGGCCAGCCGCCATCAAATCAAACGAGGC13.0303030303030303No Hit
CACCTCGACGGGGCGCCGCATCCAGGCGGGCGGCTCGTGCGGGATGCGAT13.0303030303030303No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)