FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n01_Tr4_C2I2c_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n01_Tr4_C2I2c_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107
Sequences flagged as poor quality0
Sequence length251
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATAATCAGCCGCTTCGGCCATGACCACGTCTTTTTCGGTGAAAAAGG10.9345794392523363No Hit
GCCTATAATGACGCGGTCGAAAAACTGACGATGAAGGGTAATATCGCTGT10.9345794392523363No Hit
GTCTTTCCACGGCGTTAGCGAGAGCGCCACGACCCTCTCCATCGCCCCTG10.9345794392523363No Hit
GATCATCATCAATGAATTCGGCGATGTCAGCATCGATCACATGCTGGTCG10.9345794392523363No Hit
CCTGAATGGTCGGCACCGAATGGCGGTGCTGGGTGATGCCCATGGCCCAG10.9345794392523363No Hit
CAGCAAGTGCACGTGACCCAACCCCTTTCAGGACGTAGCCCTTCGCTCCA10.9345794392523363No Hit
ATGATGAGTGCAGCGGATGAGCCGCCGGCAACGGCGCTCATCGGGCCGGA10.9345794392523363No Hit
ATGCAGTTCCCGGCGAAACCCCGGACAGCCCCGAGCCGATTCGCCGTCTG10.9345794392523363No Hit
ATATACGATCAAGCTGTTGGCGTTTCTGGGGCCACCAAAGGCAAGGATGG10.9345794392523363No Hit
GACCGAAACTGATCCAGCCCAGTATCTCGCCTGCTTGAGAATCCGGTGCC10.9345794392523363No Hit
GCCTCAAGATTTGTAGAAAACCCGGTTCTGCTGCGAGAAATCGCCCTGCG10.9345794392523363No Hit
TAATGGAAGCCTGCTCTATCTTGGGTCGGCGAAAATAGAACCGACTTGAT10.9345794392523363No Hit
GAAGAAGTACTGGGTGATCGCGCTATCGGCACCGGCCTTGGCCTGTCTCT10.9345794392523363No Hit
GATTTCGCGTCATGGGCACTCTTACGGTCAACCAGAACAAACTGCAAAAG10.9345794392523363No Hit
GGACCAATATGTCCAAGCGCTGAATCAGCAGCAGCGGGCGCTGGAGCTCG10.9345794392523363No Hit
GGGTATTCCTCGCTTTAATTTGCGACAATGGGAGGAACATATGCGCAAAT10.9345794392523363No Hit
GTACCACGTCGCAGAGCCGGAAATCGCCTGAAGCCATCGCTTAACTTTCC10.9345794392523363No Hit
GCTGATGGGCTGCATGGCCTATCTGTCGTCGCCGCTGTGGCTGGCGTCGC10.9345794392523363No Hit
ATCCTGTATCTCGCGAGTACTCCTTTGCGCCAACGTGCAAATACTGGCCA10.9345794392523363No Hit
CGTCCGTACATGATGGCGTCAGTGAAGTCGGTAACGGCGGAACAACCAAG10.9345794392523363No Hit
CTATGAAGAGGGCGCCAAGCTGAAGGCGCATTGCGAGGCGCGGCTGAAGG10.9345794392523363No Hit
GTCGCGGATGTATGCCTGGATGCGAGATTCGGAATCCGCATTGTCCGTGC10.9345794392523363No Hit
GCCAAGCGCTGCTCCCATCAGCGCAAGCGTACCGGGCAGTCGCTGGCCGA10.9345794392523363No Hit
TCCTGATCCTGATGGCCGACCAGTTCAACGGGACGTTTTTTCCCGATGGT10.9345794392523363No Hit
TCAGAAAACTGGCAAGCAGCCATTTGAACAGGCCAGGTTCGCTGGCGGTG10.9345794392523363No Hit
CTTCATTTCAACATCCGGGTTTAAATCTTGCATTAAAAAGCTGTTATCAA10.9345794392523363No Hit
TGCGATTGCTGCGCATGGAAATCGGACTGATAGAGCATGGAGATGAGGAT10.9345794392523363No Hit
GACTAATATAACAGGGCAACCAACGAATCTTCGCAACCGGATGTTCCGGA10.9345794392523363No Hit
TGGCGCGATGGATCACGCCGGTGGATCGGGATCTTCGAAAGCTGATGGAC10.9345794392523363No Hit
GCTCGCCACCGTGCGCGAACGCGAGCTGATGAATATCCGGCTCTATGACG10.9345794392523363No Hit
TGATACAAGAGCGGTCCGCGCTTTTGGCCTGGCGGCCCAGGGTCGTCTGT10.9345794392523363No Hit
TTGTTGCGTTGTCGGGGAATTATTCTCGCAAATGGCGAATTCTCAGAAAA10.9345794392523363No Hit
CGGCAGAACTGGGCGGTTTCAAGGCCGTATTAAGCTGGTCGGAAGGCGAA10.9345794392523363No Hit
TCCTTTACCAGCGCTTCGGCCTCTTCCGCATTGTCGGCATCGCCGAGAAT10.9345794392523363No Hit
CCCCAGTGCAGCGGGATGCCGATCTGATGCACGACCTGGCCGTTGACCTG10.9345794392523363No Hit
CATGCAATCCACCATTGTCATCGTCAACCTTCTCGGCGCAGTCGCACTGC10.9345794392523363No Hit
GATAACCACGGTCGTAACACGGCGATCATCCGGCAAGAGAACGCTTTCCA10.9345794392523363No Hit
TATTCGCCCATGCTTTTCTCCATGCGCCGCCCTTGCCGCACCGTCCCGGA10.9345794392523363No Hit
GGATTGCATGGCATTTGACGCCACATTGGATATCGGAACGAGGCTGCGGC10.9345794392523363No Hit
CTATTTCCCATCGTCGGACAATTGCGTATCGTGCGAACATGGGCGGGCAT10.9345794392523363No Hit
ACAAGGCGGCGGCCGGCGGATCGGCTATCCTCGTGCAGAAGGATTCGCCG10.9345794392523363No Hit
GTGTTGCTCGCTTCCGTCATTTTATGATCTAAAATTTGCATCGACATGTC10.9345794392523363No Hit
GACCACTTCTTTCAGAAGAAAGGCATGACCCTCTGCCAGCTCGCGATGAT10.9345794392523363No Hit
CCCAAAGATCGTTCCGTGGGCATCACACCGTTCCAATCGCCCGATATGAG10.9345794392523363No Hit
ACGAAAAGACCCATCACCTGCGCGGCTGATTTTCCGCGCATCTAGATTTT10.9345794392523363No Hit
CCGCGTATTCGAAGGCAGATCGCAAATTCGTTAACTTCTGAGCAAGGATA10.9345794392523363No Hit
CAAGGAGATCGGCCAGCCCTTGCGCCCCAATGACATCGCCAATGGCATCG10.9345794392523363No Hit
AGATAACGCTGCACCTGTTTCAGGCCCAGACTCTTCTTGTCGGGGTAATA10.9345794392523363No Hit
GAATAACACACGGTTTCGCCGCCCAGTGCCGCCGGAATGAAGGGGTGAGG10.9345794392523363No Hit
ATCAGCAACTGTCCCTGCGCCGGGCCAAGAGCGTCGCCAACGTCCTCGGC10.9345794392523363No Hit
GGGGAAATGCTCTACCTTTATCAGGCATTTGATCTACGCAATAGCAGCGG10.9345794392523363No Hit
ATTTATAATTGTTGTAACCCTGTTCGGTATGCTTGCTAATGCAGCACGAG10.9345794392523363No Hit
TCAAAGGCCGGATCTGGCCCGAGAATTCCACCCGAAACTGCCCAATGCCG10.9345794392523363No Hit
AAGGACATGATGAGATCATCCATGGCTTCGCGAATGGCGGGATCGTCGTC10.9345794392523363No Hit
GACCTGATCCCGTGAACACAGCCATTAAATCCGTCAGCGACAATTGGACA10.9345794392523363No Hit
ATAAGAGCCAGCAGGCCGAAAAACAGGGCGAACACATAGGGCAGGGCAGG10.9345794392523363No Hit
AAATGAATGTCGAGCAGCGCGGCCGGATAAGCGCTTGCCGCATGGCCGCC10.9345794392523363No Hit
AAATACGAGAGGAAGGATTGATTCAACTCGATGAGCCTATTCATACTTAC10.9345794392523363No Hit
GATCTGAAGGCCGAAGGCCGGCCGCTTGCGAAGCTGACGAAACGAGAGGC10.9345794392523363No Hit
GTATCGCATTCAGGAAGTCATCAAGCGCCGTCAGATCCTTCTGGTGCAGG10.9345794392523363No Hit
ATCCAGCAGATGGTTCAGCAGTTCGGTGCGGTGGTGCGAAGCGATACTAT10.9345794392523363No Hit
CTTTGTTGTCGTTGATTGTGAGTGATTAAAACCCGTCCCAGCAGGCCTGT10.9345794392523363No Hit
GTGAAGTGCTTGGTGTCAAACGAGTAGGAATTCATGATTCATTCTTTGAT10.9345794392523363No Hit
CAGCTTGCGAGACGGCATGGCGAGCTCCTCTGTTATTCCGAAACAATTTA10.9345794392523363No Hit
CACCAGGTTCAGATTGGCCTCGGCGGCGGCGACCTGCGCGCGGGTGGCCT10.9345794392523363No Hit
AGATGAGATAGCTTGTGGCGCCAAGATAGCCGAGTGAAAACAGGCCGATC10.9345794392523363No Hit
ATTATATCGTAAAACATTGAAAAAAAAAGTGTTACAAATACCCTTTTTCC10.9345794392523363No Hit
GTAATCGTCCTGCTCGACGGCGGCCAGGGCCAGTTCCAGGGCCTTGTCGG10.9345794392523363No Hit
ATCTATGTGCTGTCAGTCTCGCTGTCTTCAGGCGCCGCCGTTACGGCTGT10.9345794392523363No Hit
GCTTTTTTCCCCGGCAATGGCAAACAACCGGGCGTGGACTAACCTCACGG10.9345794392523363No Hit
GGGAGATGGGGCGGGTCATGGCATCTGTTCCAGTTTTCAAAAGCGGGTTC10.9345794392523363No Hit
ACCCAACGCGCTGGAGCTTCTACTTTACCGCAATCATGGCAAAATTCGAG10.9345794392523363No Hit
CTGCAAACCGTTACCGGCGCCACGAATACGCAGGCTGTTCGGCTGCATTG10.9345794392523363No Hit
TCGACAATGAGCGTTACCAGAAATGGTATCAGGAGAGCGTCGAGGATCCG10.9345794392523363No Hit
TGGCTATATCGATCGTTTCGGATGCATTTTTCGCAGCGATGATGACGCAA10.9345794392523363No Hit
TCCCCATACCACTTGTTGATGTTTTTGATAGAGATCATACGGCGAACCTT10.9345794392523363No Hit
CATATCAGCTATCGAGAAGTTCGGCCATGACCTTGTTGAAACGATCGGAT10.9345794392523363No Hit
CACCATAACCGATGAAGATCAGGCCGAAAAACGGCAAAAGCAATCCGACG10.9345794392523363No Hit
TCATTGATGCGCTGGGCCATGACGCGGCGAGCAGCGGCGTCGCGCCGCAC10.9345794392523363No Hit
TGCGTGATGCGCCTCATTTAACGCGTCTTGTGCTTCCTGCAATTTTTGCT10.9345794392523363No Hit
GGTTTTCCCTCAATTGTAGTGGATGCGCTTTTCGATGAAGCGGAACTGGA10.9345794392523363No Hit
CCATAATTATTGCTATTTCATAATTAGCATAAAGATGGAAACGTGGACCG10.9345794392523363No Hit
TCTCCACACCGTTTGCTGCCTTGTATGGAATGCCTGCATGTCGACCCTGA10.9345794392523363No Hit
ATTCCCGAGACCAATGCCAGCAAATCCAGCCGCGAAAGGGTCATCGAGCT10.9345794392523363No Hit
ATATGGGGTTTCAGTTCCAAATGGTAAAGTAGCATTTACACCTGATGAAG10.9345794392523363No Hit
GCATAATCGATCAAGGTCGTATCGGTCTCATGCCCCACGGCGGAAATCAG10.9345794392523363No Hit
TCGTTAATCGCCGTATCGGCCAGGTGAACGCGGTTTCCTTCGTTGCATGG10.9345794392523363No Hit
GTTCCAGTAAGCGATGAACATCTCGCCACGGTAAAATTGCGGTATGACGT10.9345794392523363No Hit
GTGGAAGCACCGCGCATGACGTTGATGGCGTTGACGTAGAATTCGCCGGC10.9345794392523363No Hit
ATAATATTCCTGTTCCCTACTTCAACAATTTCGCCCGCCAGGGCCAGGCC10.9345794392523363No Hit
GAGATGAGGTCAAAGCCTCCGCTTGATCGGTCGTCAGGGCCTGAAGCTGG10.9345794392523363No Hit
GCATGTACGGGCTTGAAGTTGTCCCGCATGGCTTGCTCTTCGCCCTCTTT10.9345794392523363No Hit
CTGTTAGATCATAGGGCGATTCCGTGCCAAATTGAAACGTTTTAATCTTC10.9345794392523363No Hit
ATCCGAATGTTCCGCAATTCTTCGGTGACGCTCCGCTGGCCGGCTGGTAA10.9345794392523363No Hit
GAACTGGATGATGGAAGGCAGATCTGCCTGCCCGTCCGGCCGCTTGCGGA10.9345794392523363No Hit
GCATGATCGCCGCAAGCTCCGCGCCGGATCCGGCGATGTCGTCTTTCGTT10.9345794392523363No Hit
ATACATCGGCATGGTGGTGACATCATTGCCGTTGATGGCGATCTTGCCGG10.9345794392523363No Hit
ATCCTGCCGCGCGAGCGCCTCGAACTGGCCCTGACCCGCATTACGGTGCC10.9345794392523363No Hit
CTGATGCAAAGGGAGAGCTTGATTTTGCGATCGGTTCTCACCGGCTTGGA10.9345794392523363No Hit
CAGCACGCCCAGCTCCGCCGCCCGGCCGGTGCCAACCATGATAGAGGTGG10.9345794392523363No Hit
GACGCTGGTTCTGGCCATCGCAAGCCTTCTCGCCACCTGGGCCATCGCAC10.9345794392523363No Hit
TTCCGCGCCAGGCGCTGCATGCCTATCTGCTCGCCTTCGAACATCCCCGT10.9345794392523363No Hit
ATATATATTTCAATGATTTATTCTTCTTCTTTAACAACAATGTGGCGAAT10.9345794392523363No Hit
ATCTTGATGGCGTTTCCGTTTCTTTCGATGGTTTCAAGGCGCTCAACTCG10.9345794392523363No Hit
AGTGCGGAGACGGAAACAGTTCGAGGAAGGCGAAGAACCAGTCGCTGCGT10.9345794392523363No Hit
GCAGACAACACGCTCACCGGCAACATCGCATCCGTCGCCTACGACCTCGA10.9345794392523363No Hit
CGGCATTGTCGGCGACCGACATATGCGGCCACAGCGCATAGGACTGGAAC10.9345794392523363No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)