FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n01_Tr4_C2I1b_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n01_Tr4_C2I1b_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49
Sequences flagged as poor quality0
Sequence length251
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCACCAATGCGGAGTGACGATGAACCCCTCAGACGAATTTCTGGAGTC12.0408163265306123No Hit
GTTCTAAATGATAGAACAACCCAAGCGGATTTGCAGCGATTATTTTTATC12.0408163265306123No Hit
CCTGTCGATAGCGGCGTTGCCGATTATGTCGGTGGTTTCGTGGTGACGGC12.0408163265306123No Hit
ATTCTCTATTGTGACGACGGCAGAAAAACCGGCACGGCCGGAGAGGGTCC12.0408163265306123No Hit
CACCCGGCCTTCGCGTCGGCTACATCGTCGCGTCTGCGCCTGTTATCCGC12.0408163265306123No Hit
ACGTAGCAGATGCCCAGTTTTTAAATGCTCAAGTCATATCCTGTCTCTTA12.0408163265306123No Hit
GGATAAATTACGCCGTGATGCGTGAAAGATTCGCTTATTGGGCGCAGCAG12.0408163265306123No Hit
GAATAACCGAGCCCACGAGACACTCACTGATCTCGTATGCCGTCTTCTGC12.0408163265306123Illumina PCR Primer Index 5 (96% over 26bp)
CGCTTGAGGAGTACCTTATGAAGATCGACCCCCGAATCAGCGCAGAACTC12.0408163265306123No Hit
GGTTTCATGATTGTACTAGTGCAATCTCTCACAATTAAGGAGGATATACA12.0408163265306123No Hit
ACCATCTCCCTGTCTGTTAGCCGCCGCGATTATCGCCCGCTTGCCTGACC12.0408163265306123No Hit
CCCATTCTCGGGCTGAGTATTTGTTGAGGACTTTTCAACATAAGTGTATT12.0408163265306123No Hit
ACAATGACTAGGCCGTTTCCACGCTCGCGAATTTCAAGACCGTTCGCACT12.0408163265306123No Hit
ATGTCGAATATGATTCGACGTCGCTTTCGGATGAGTATTTCCGGGACGCG12.0408163265306123No Hit
GGCCCGGCTGGAGACTTCATTTCCGGCACATCCTTGCACTCATATGAAAG12.0408163265306123No Hit
GCTGTCGGACGATCCGTTGTCGGTCAAAGGCGCCAACGGTCAGGTGTGGA12.0408163265306123No Hit
TCACCGAGCCGATGATGTTGACCTGTGCCAGGCCGCCTTTCATCCAGCCG12.0408163265306123No Hit
CGCTCTACCAGGCCATTCTGGTCGATCGGAACACCGACTGGGACAACCAG12.0408163265306123No Hit
GAATCAAATCGTGGGAGACGAAGTGCCAAATGGATGCAATTGCCGTTCAA12.0408163265306123No Hit
CGTTTATTAGAGCGTGCAGCGAAGCTTAGTGATGAAAAGGGTGGCGGTTC12.0408163265306123No Hit
ATTTCAGATGCCTCTTCCGCAGGTGCAATTGGCACGAAAAGATGCTGAAC12.0408163265306123No Hit
GATTTCCTGCGCGGCAACGAGCCTTACAAATATTTCTTCGGGCCGGAGGA12.0408163265306123No Hit
CAGCATCACCCGGCGCGTGGTGCGGTTGAGAAGTGTGGTGTGCAAATGCG12.0408163265306123No Hit
ACCTTCATCATGGCAGCGAAGCCGTTCGAGAGCTGGGAGAACTTCGGCAA12.0408163265306123No Hit
GTGTAGTCGCTGATCCGGCCCACGGCCATGCGCGGCGAGGGCAGGTTGTA12.0408163265306123No Hit
CTTTCACCGGAGAAGATAATGACGGTATCCGGAGAAGAAGCCCCGGCTAA12.0408163265306123No Hit
TGCTGCGCGTGCGCGGCCTCGGCGTCATCACCAATGACAGCGGCAGCGTC12.0408163265306123No Hit
AGCCACATGTTGCTATCCCTCCCTGGTGGTGAGTCGAGGAGCGTGTTGCT12.0408163265306123No Hit
GCCTGAACCTGGGCGTGCCGGTGATTTTCGCGCGCAAGCAGCAATCCCTG12.0408163265306123No Hit
GAAAGGCCGCATCCTCTCGGCCATCAACGATACGCGCTACAACTTCATGT12.0408163265306123No Hit
TGACAGAGCTCAGCTCAGAGGTCATTGTACTTCGTTACTTTGAAGATGGT12.0408163265306123No Hit
CATCAACGAAGACGTTCTTCGCTACATGACCATCGCCGTCGAAGCCCACG12.0408163265306123No Hit
GTAAAGGACCAAGGAGGATACCATGAACAAGGACGAAAAGCTGAAGCAGA12.0408163265306123No Hit
GTATAGATATACAAATATATGTATTGATTGAATTTATATAGGTAAAATAT12.0408163265306123No Hit
TCCGGGGCCTTGTCACCGTAGACCGATTTTACCCACAACAGCAGGCCGAG12.0408163265306123No Hit
TAATTAAACGAGTCGAGAATTACGGCTCATTCGCCTGGCTTGATGACCAT12.0408163265306123No Hit
ATCCCATTGAGCCGAAGCGGGCGGCGACCTTGGAGGTACGCGAGCGCTCG12.0408163265306123No Hit
GGGTGGCCGGGTTGGAGACAAACGCCATGGCCGCGTAGTAGGCGATTCCG12.0408163265306123No Hit
GCCCAAGGCGGACGTCGCCGCCCGCGTCGAGGAAATGCTGCGCCTCGTCA12.0408163265306123No Hit
GAATTAATAGATTTCTTAATTCTTCATTATAGAGAGAAAAGTCATATATA12.0408163265306123No Hit
GGTGATGTTCATCCTCACCGCCACCGCGCAGGTCTCCGGGATCCAGATCA12.0408163265306123No Hit
ATATCATGCACATGGCTGGTTCGATGCGATAAGAAGGCACCAAACGGAGT12.0408163265306123No Hit
CACCAATTCCAAGGGCGTGATTTTCTCCCGCACGATTTCGATCGACGAGC12.0408163265306123No Hit
GCGGATTACAAGGCGCTGATCGCGGGTGAAACCGCCCGTGAAGACGGCGT12.0408163265306123No Hit
TACGGGTGTTGCCACGGATTTCGATGCGCTCGACATAGGCGCGCTCGCCC12.0408163265306123No Hit
GAAAAGCATAAACCTAGCTCAGTCTTTTCTAAACTGTGCTTATACGCAGC12.0408163265306123No Hit
TTCTTGACGCGCAGGCGTCACTCTTCTTATCTCTACCTTACTACCTGTCT12.0408163265306123No Hit
AACACGCCCATAACACCCCAACGCTTCCAACGGTTATAGAGCGTCTTGTG12.0408163265306123No Hit
CAATATTTAGGTACGTTCTATCTTTATGGCTTCCTGAAGGCCTTAGAAAC12.0408163265306123No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)