FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n01_Tr4_C1I2c_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n01_Tr4_C1I2c_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123
Sequences flagged as poor quality0
Sequence length251
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGTAATAGGCCGTGATCAAGTATCTATGCTGGGGGTGTTGATCGATC10.8130081300813009No Hit
GCTCTTCGCTTGGTTATCGAGCTCGCTGAGCGCCGTATCGCAATCGAGTC10.8130081300813009No Hit
CAAGTGCTCCGGCCAGGTCGAGCCACCGGTTTCGCCCGGATGCTGAATGC10.8130081300813009No Hit
GATAACGCTCGGCGCGGGTCTAGACTTCTTCCTTGGTCGGCTTGCTGCCG10.8130081300813009No Hit
AATAACGGATATGAAAGATAAGGTGTAATTTAGTTAATAGTATGATATGA10.8130081300813009No Hit
GTCGTAGTTCAGATCGGTACCGATCAGGTTGATCATCACCGACGGGCTGT10.8130081300813009No Hit
GTACTTACTAGTCCAGCAGGGCGATGATCGCAGTGGGTGCGTCTTCGCCG10.8130081300813009No Hit
CGCCGACTACTGGTACAAGTACGTGGGCCTGGAAGGCCGCGTGATCGGCA10.8130081300813009No Hit
CCCTACATGGCTGTTCCAGATGACCCCTCCAGCGACGAAGCGTTGCTGGC10.8130081300813009No Hit
GCCTTCTGCAGATCCAGCAGGTAGTAGTTCCAGTGACCGTCAAACGCCCC10.8130081300813009No Hit
CTACAGAATATAAGAAATCTGCTCCTGTTTTATCCATTTTGTTAATGAAT10.8130081300813009No Hit
GTTGGATCAACACAGACGATCAGGACTTCAGCTGAGTTCAGTACCGTATT10.8130081300813009No Hit
GACAAAGACCGCTTCCGCCAGGGCCTCGGTGACGTGATCGAAGCCTACGA10.8130081300813009No Hit
ACGAGGAATTGGCTTACTTCCAGCGCGACTACGTCGGTCGCCCAAGCCCG10.8130081300813009No Hit
AGCAGCGCCTTGTAGGTCGCGACATCAGCCACGCCCGACAGGGTCAGTAC10.8130081300813009No Hit
TTCATTGTCAGGGGATTTATTATTAGATGCAGCGAGTAAAGCGGTTGCAA10.8130081300813009No Hit
CCTAAGGGGGCGCCCCTACTGCTTGGTAACAACTTGTAAACAATCTCCGG10.8130081300813009No Hit
GGCAAATACCGTATCAAGATGGCCGACCAGCAGAACGTCGAACTGTGCCG10.8130081300813009No Hit
ATGATGGGTTGTAAAAATAGTTAAAAACGGGCCGCTCTGCGGCCCGTTTC10.8130081300813009No Hit
GATCTTTCATCAGGGAAATAAGAGGCGATACCACGACCGTCAAGCCGTTG10.8130081300813009No Hit
ATAATATTCTTCATCTAAGTATTTCCAAACAATATCAACCTTAATGTTTT10.8130081300813009No Hit
ATGTTTAGCCTTAATGCTAAAGATTTATTAATATTTTGGGTTATTTCTAT10.8130081300813009No Hit
GTGCCGTACGGCACGTTGCTGTGTGTATCGGATAAACCGCTGCACAGTGA10.8130081300813009No Hit
GTATCTCACTGACCTGGAATTTATATAGTGACCCCGTTGTCACCATGGCA10.8130081300813009No Hit
ATCAATGGGATAGTGCGCAACCACTTCGGTGACCACCTGCGTTATCCAGT10.8130081300813009No Hit
GCCCCGGTTGATACTGCTGCGCCATGCTGCCTGACTCCCGCCGTGAAAAA10.8130081300813009No Hit
GGTCTCGCCCGTTGTCACAATAAACAAGGCTTTGAGTATCTGCGGTCTGT10.8130081300813009No Hit
ACGTGGTCGAGCAGGCGATGCTGTTGTTTCGCTCGGCCGACCAAGTGGAC10.8130081300813009No Hit
ATCATGTGTTTCAGTAGCGTGTCCCCTTCGTCTAGTGGCCTAGGACACCG10.8130081300813009No Hit
CGGCTCATCAGCGAGGCCGGATACATGGCACGATACGGCTCATTGAGCTG10.8130081300813009No Hit
ACCAATGATCAGGCTGCTCAGAAGTGAGATGGCCAAGGCATAAGCATCGA10.8130081300813009No Hit
GTATCGAGCTTCATCCAGACGGTGACAGGCTCACTCAGTTCGGCAGTTTC10.8130081300813009No Hit
GGCTTATGCCGCCCCTTATTCCATACTGATCCCCATTATTTCCTTGCAGT10.8130081300813009No Hit
AAGAAAGACAGTACACAGCGTGATTACAGGTCATTGAAAAGGTTAGTTTC10.8130081300813009No Hit
CGGCAGCTCCGCCGGGTCCGGTGCGGCACCACTCCAGCGGGCCGGGGACA10.8130081300813009No Hit
TTCATGCACAGGCTGAATGCCCTTGCTCCAGGCAACACAGCAAACGCTCC10.8130081300813009No Hit
GATCAAATTCGCCGCCATGGCGGGCGCGATCGATGAAGAGAAAGTGATCC10.8130081300813009No Hit
CAATACACGCCCTGCCAGGTCCAACGCCAATTCGCACAGGAACATGGCGC10.8130081300813009No Hit
CCTTGAATAGGCCGAACAGTTTGCTGTCGGAGCGCGACTTTTCCGAGTGC10.8130081300813009No Hit
AAAAATCACTTTGTAGTCGGCACCGTACTTGTGCAGAAAGTCCTGTCTCT10.8130081300813009No Hit
GTGATGGATAACGAGGTGTACCTGGCCGGGCAGGCCATGCCGGACCTGCT10.8130081300813009No Hit
ATGGAGAACCCTTCGTCAAAGATGGGGGAGATTAAAGAACCAACCGATTA10.8130081300813009No Hit
TCAAGATTGAGGAGGCGAGTCGCCCTTGAAATCCTGGTCTTAGCCCCCAA10.8130081300813009No Hit
ATGTTGTAGGTAAAGGAAATACCCGATACCCGCACCGGCGCCGGAAACAG10.8130081300813009No Hit
TGATAAGAGCAATGGCAATAACCGTCATCGTGTTTGTTAATATTGGTTTT10.8130081300813009No Hit
AGCGAGCAGAGCCTGAAAACCTTCCGCGCGCTGTTTATCAACCTGCTGCA10.8130081300813009No Hit
GACGAACCTGGCGCCCTCCCATGAAAAGCAAACCCTCACTGCCTCTGGCT10.8130081300813009No Hit
GATAGTTTTGTTCCTCGTCGAAGCCGCGGGTCCAATCCCTGTTTGCCAGG10.8130081300813009No Hit
GCTCAGGGCCTTGCTGCGCATTTCGTCACCGTAGGCCAAGCCTTCGGCGC10.8130081300813009No Hit
GTACCTCGTCCGAGGCTCCAGAGAGAAATACACGGATGTAGTCACGTGCC10.8130081300813009No Hit
CCTCTAACTTCCTTGTGTATAGTCTGGATGAGGAAGTTGAACCTGGGAGC10.8130081300813009No Hit
CTCATTGCCGTGTTCGACCTCACGTACCGTGCGAAACCCCAGCCTGGAGT10.8130081300813009No Hit
TCCTATCGCTTTGTGCTGGTAGGTATCGCCACGCTGCTGCTGTCGCTGAC10.8130081300813009No Hit
GTACTGTAATCTCAGATAGTTCACCGATTGTTGTGAAGCTGAAAACACCA10.8130081300813009No Hit
CCTTGAATACGGTGCGTCAGACGCCGGTTCAACCCGATTGAAAACTTACT10.8130081300813009No Hit
GATCAACAACGGCACCACCAACACCACAACGCTGGCCGAGACGCGCATCG10.8130081300813009No Hit
CAGCAGGATGTGCTCACGGGTTTGTGGCATCGGACCATCAGCGGCCGAGC10.8130081300813009No Hit
CACCAGCGAGTATATGTCGATGATCCACAATAATTCGTTACCGCTGGCAC10.8130081300813009No Hit
GTATTTTTGTACCTCACCTCAATCAAAATCAATAAAGCGGTCTATAAATA10.8130081300813009No Hit
GTCCAACCATAGGCCAGCGTCCGATGTGTACTGTTCAGCTTCCATATCGT10.8130081300813009No Hit
TGGTGAATAAGTTCACTATTACCGAAGCAGAGCAGGCGGCAGACACGCTG10.8130081300813009No Hit
ATTAATGTACCGAGGAGTGTTCCGGCAATAAAACCAATAATCGTTTCCAT10.8130081300813009No Hit
GGAACTGGCTGCCCTGTTCGGTTGCCAGGGTTTGCGCCAGGCGATAGCCC10.8130081300813009No Hit
TCACTCAGCCCCGTACCCGCCACGAACAATGGTTACTGCAAACCGTTGAA10.8130081300813009No Hit
ACCTTGTACGGGGTGAGGATCTCCCGCGCCCAGGCTCTGAGCGCGTCCGC10.8130081300813009No Hit
GTGGTGTACGGCGCATTGGAGCCTAAGGCGGGGATCGTGCAGAGCCAGGG10.8130081300813009No Hit
GTTTAGCGTTGGCGAGATTCTTCCGCGCGCCGCTGTGGTCGCGATAACCT10.8130081300813009No Hit
GATAAGCCGCCGCGTTTTTGTCGAGCAACGCGTCGCCGTCGATGCACACC10.8130081300813009No Hit
TACTTTTGCTTAAAATAGTAAGTGGCAAAAATTTAAAAGGATGGCTAATA10.8130081300813009No Hit
CCTCAGACGGTGCATTGCCGGCCGTCTGCCTGACCTTGGGCCAGCCTACA10.8130081300813009No Hit
CTGCTGTGGGCGCTGTCGCTGACCATCATCCCCGGTGCCGCCGGCTGGCA10.8130081300813009No Hit
TTGTTGCGTAGCATTCTCATCTAGTAGCGCTCGCTCCCTGCGAGCCCATC10.8130081300813009No Hit
GGTCTGAATCGCGTGCATCTGCACGGTGGCCAGTTCCGCCAGGATGTCCG10.8130081300813009No Hit
GTTAACAGCAATAAGCCGATGGACTGGGACTGGATTAAATCCTTCCCTGA10.8130081300813009No Hit
TGTCTACATCACCGAGCAGGAAGCCGACGCCCTCACCGTGGCCGGCGCCA10.8130081300813009No Hit
GTGAAGACGTGATGTTCGACGAAACCTATGTGCATTGGGTCAAGAACGAG10.8130081300813009No Hit
AAACGAGGTTACTGAATTCATCGTAACCATAGGTAACTTTTACACCCGTT10.8130081300813009No Hit
TGACTATATAGGGCGTTATGCCCTCCCCTACACCAGACCGTTGCGCTGAG10.8130081300813009No Hit
GCCACACGTCGATGGCGTCGTCCGGTCCTCCGGGCGGCGCCATCGCGCTG10.8130081300813009No Hit
GAACCGCTGTGGACGGTTATTCCCGGCAACAAGGCGATCCTCCCGGTGCT10.8130081300813009No Hit
TTCTGCAGGTGTTGCACGCTGACGAAGCTCATTGGCTTTTGTCCTTGTTC10.8130081300813009No Hit
TTCCAGCACGGCGGGCAGCTCGACACCGACCGCGACGGGCAGGTGGAAGA10.8130081300813009No Hit
CGCTCAAAGTGCGCCTGGCCGAAGACAAACCGGTGCCCGAACTCAAGGCT10.8130081300813009No Hit
GTGGAAAGCAGCAACATCCTGCGCGTTGGCGACCGCCAGGGCACGCTGCT10.8130081300813009No Hit
ATCGCCGGGTGCTGATCGACAGCGCCAACAACCTGGCCCGTCGCATCAAC10.8130081300813009No Hit
GTATGTTCATTGCCACGCTTAGATGTGTCAAAATACAACACTGTGCCATA10.8130081300813009No Hit
CCTGGGGCATCCGCTGGGGCTCGGACACGGTGATGGACTTGTCCACCGGC10.8130081300813009No Hit
ATACCGAGCAGGCCCAGTTCGCCCATCTTCGCCACCAGGCCATCGTCGAT10.8130081300813009No Hit
CCGTTATAAAATAGCCAGCCCCAACAGTGTGATTGCGATCATCACCTTGC10.8130081300813009No Hit
TTGCTACGGCGTCGCTCTTCGCTGCGCTTGCGCTTCCACGCTTTGAAATT10.8130081300813009No Hit
CCCACATTGATCTGTGATCGACATGACAACTCGGTCAACTGTGGGAGGGG10.8130081300813009No Hit
GAATTAGAAAAACGTGGTCATAAGTTCGTTCGTTACGCAGATGACTGTAA10.8130081300813009No Hit
GTGCTGAATATCTTCCCAAGCGTCGACACCCCAACCTGCGCCACTTCGGT10.8130081300813009No Hit
CGTTCCTGGTTTACGCCAGTTTGCATGGTGCTGTGTTTTGTCTGCCAAAA10.8130081300813009No Hit
GTATATGTCAATGCGGATATAGATGGACCAACCGTAGCACTAAAAAGAAT10.8130081300813009No Hit
ATGGACTTTAGCACCGAAGTCGGCGCTGATGATGCCCACGGCTTTTCGAG10.8130081300813009No Hit
CCCAAATTAGATCTGCACCGGTCCTAGGCGCTTTTTACCAGCTTCACCGC10.8130081300813009No Hit
GCCCTGGCCGAACTCGATACGTTGCAGCGCGGCCTTGACGAAGTCCGGCA10.8130081300813009No Hit
CTCAAGACGTCGGCGATGATCGCGTCGTTCGCCGACCCCGGTCAGCGGAC10.8130081300813009No Hit
GGTATTCAGAACGTCTGCTGAAGCTGATTGAGAGCATTGTTGTCGAGGGC10.8130081300813009No Hit
GTAATAAGCAGACAGCGGATGGTGTCATCGCGCTCGGACTGCTTCAGGCA10.8130081300813009No Hit
CAATAAAATCGATCACTTCCTTGAGGCAATTTTGGTGGCCGTCGGCTCCA10.8130081300813009No Hit
CTGAAAGAGCGTTATCACTACGACGCATTCACCGTGCCTTATCCGACGCT10.8130081300813009No Hit
ATGTCATATCAGGGGCTTTTTGTCGCAGAGGAATCAGCCTTCTGCTTGAA10.8130081300813009No Hit
TCGATCACCTCTTCCAGCAGGTAATCGCGGATCAACCCTTCGCCGCCGTG10.8130081300813009No Hit
AAGTAATAGTTGGCGGTGTTGATCCGGGTTTGCGTGGCCCAGACAAACAG10.8130081300813009No Hit
TCATGCTGGCTTTTGCCACAGAAGGAGCAATAGAGCAGCTTGCCGTTGTC10.8130081300813009No Hit
CTCCCCTACATTGCCCCAGCGGTCGATACCACGAAACACCGGCCCGCGCA10.8130081300813009No Hit
AGACGGGATCATCCTGGGCAACGACGGCGCGGTCTACAAAGCCGGCTGGG10.8130081300813009No Hit
CACTGGAACTGGGCCCGCTGAACGTCGACATGCAGAACTTCGACAGCCTC10.8130081300813009No Hit
GGGTATATCGAACGCTACATGAGCGGCGAGTTGTCCCGCGAAGACCAACG10.8130081300813009No Hit
CACCTGACGTTTGCGGGTCAGGCGCAGGCGCCACGGCTGGCGCGGTTGCA10.8130081300813009No Hit
GTGCTGATGGCGTGATTGGCCAGTCAACGCGCGACTGGCTGAACGTCTCA10.8130081300813009No Hit
GAGTATCTACTGTCAGAAATAACAGCTTTGCAGGTGTTTCAGACGAGCGG10.8130081300813009No Hit
GTCCTATAAAGCACTGCCCAGCCTGGGGTCGATGTTGCACGCACAGGAAC10.8130081300813009No Hit
TCCTTGTCCTTGTCCACGTCGGCCGAATGCAGCAACTCGTTCAAATCGCG10.8130081300813009No Hit
GGTAAGTGATGTGGCCGACCATGCGCGCCAGCATCAGGCCGCGCTTGGGA10.8130081300813009No Hit
GGTCAGCCACCGCCAGCAGTTTTTTCAGCTCGGCATCGCCGTCCTGTCTG10.8130081300813009No Hit
ACGATGACGTTATTCACCTGGGCACTGGTGCACGTTACCAGCGCGCGCGC10.8130081300813009No Hit
CCAGGAAACCCCGGAGCGCGCGCTCGATTCTTGCAGCGTGGTTGAAGGTG10.8130081300813009No Hit
GGGCATGCCCGAAACCACCATCGGGTTCGCCCCGGACGTCGGTGGCACGT10.8130081300813009No Hit
GAGGTTGGTGAACCAGCCGACCGTGCGCGTCAGGTCAATGTCATCGAACA10.8130081300813009No Hit
AGTAAACGTAATTTAATTAACCGGAGAGTAAATGCGCTGCATTTGCTCTC10.8130081300813009No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)