FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n01_Tr4_C15I4c_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n01_Tr4_C15I4c_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31
Sequences flagged as poor quality0
Sequence length251
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAGATCTTCCGTTAGCCGGTCGGCCGTCACATCATTCGCGTCTGCGAC13.225806451612903No Hit
ATCCCCTTCTCCTTCCGCCAGATCAGCAGCCGCTACCTGTTACTGCTGCG13.225806451612903No Hit
GAATACCAGTAATGAAAGGATCAGCGCCAGGGTGAAGAACGCGGTCAAGC13.225806451612903No Hit
GAATTGGGCTGATTGTAAGTGACAAACCTGTTGATGAGTAAACTAAAAGA13.225806451612903No Hit
TTTCTTCATAGGTCAACTGCACCCGCGCCCGGATCAGTTGGTAGCGCTTG13.225806451612903No Hit
TCCGTGGCCGGACTGGGACCGGAATCAGTGCGGGCCGGGCTCGGCCGGCT13.225806451612903No Hit
ATCTCGGTGCGCTGGCGCCAAAGCCGGTCATGGAGGGCAAATCGGTTCTC13.225806451612903No Hit
CATCGGCATGGTGTTTCAACAGCCAAACCCGTTTTCGATGAGCATCTTCG13.225806451612903No Hit
TTCTTCCTCCCAGCATATTAGCAACTTTCATTTTTTTGCATAAAAAAAGC13.225806451612903No Hit
GTCGCGACCCGAACGGCTCGCGACATGAAGGTGGATGTTTGCTTCCGGCT13.225806451612903No Hit
GCTTACCATAAGTAGTATCAGCTTCAGCAGTAGCATAGTCGATGTCAGCA13.225806451612903No Hit
ATATACAAATGATCGTGGCTGCCCCGGGGATAAGCAATGGAAATCCACTT13.225806451612903No Hit
CTCCAAAACTCACGAAATGCCTGTGCCAGCACTTCCGATACTTTAATCAT13.225806451612903No Hit
TATGTAATTCCTATATTGATATTGTCCCAGGGCATGTCCATCTTCAGGAA13.225806451612903No Hit
ACTCAATATCGGCAACCAGATAGTGGTGCGCGGCCTGCTGCTGACGGGCC13.225806451612903No Hit
ACTTCAAGGATATCGATGACCTGCTCACCGATACCTTCGCCCAATACGTC13.225806451612903No Hit
GATGACCGATAGCCGCGCGATTGCGCTTTTTTGCGGCATTAGGCAGCATC13.225806451612903No Hit
GTAATAGGCGCCCAGGCTCTGACCTTGCAGGCTCAGGCTGTCAGTGCTGC13.225806451612903No Hit
CAGGAATGCCTAAGAAATATCCACGGCCAAATAGCTGAAATGCATAGTTA13.225806451612903No Hit
GCCATACAGCACGACCGGGCGGTCGTTCTTCTTGCAGCTGTTATCTGCCT13.225806451612903No Hit
AGCTAAAGTCGATGGAGTTGCTGGGCTAGAGACAATTTTCCTACTTAGTT13.225806451612903No Hit
ACTCAATGAAGAACTCGGCATCGATGCGCACGTGGGCGCGTGGGTGATGG13.225806451612903No Hit
GGGTCACAACTTCCTGATGGAAAGCCTGCTGCCGCTGATGCAGGCAGCAG13.225806451612903No Hit
GGATGCGAAAAGTGCAATCGAAGCGGTCATGTTTGAGACCGTGGGAGAAG13.225806451612903No Hit
TTTTAAGTATGCTGTTAGAAAAATCATTTGACCTATTGGAAAAACGGATC13.225806451612903No Hit
TCCCCGAACGCAGCGCATTCTTGCGGCGCAGATCAATGGCGTCGACACCA13.225806451612903No Hit
CTGTACATACTGTTCGGGCAGAAGGCCGGCGCTGATAACGGAGTAACTAC13.225806451612903No Hit
GAGACAGACCGCTGCGGTGCTCACCGTCAGCGCGCTTGTGCTGATGGTGG13.225806451612903No Hit
ACATACGATTGATACAAAAGGGCGCATGATCATCCCTGCTAAATTTAGAG13.225806451612903No Hit
TCCCGCTGACACCATGCAGCTGGCGTTCAGATCGATTCAAATCAATTGGG13.225806451612903No Hit
ATCGTCAGCTATGCCGAAGATGGCGCGTTGCTCACCGAGCTGTTCACCCG13.225806451612903No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)