FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n01_Tr4_C15I2c_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n01_Tr4_C15I2c_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123
Sequences flagged as poor quality0
Sequence length251
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGGCACCTACGCCGGTAGCCCGATCGCCTGCGCCGCGGCACTGGCGGT10.8130081300813009No Hit
AGCCTGGGGAGTTCGAGGTGGCGGTCCCAGTTGTCGGTACGCGGCTTGCT10.8130081300813009No Hit
GGCTGGCAGCGAATCTCCGCCTCGTATTGTTTGCCTTCGGTGGGCACGAA10.8130081300813009No Hit
GACCTGGTCCACGTCCTGGAGCACCCGGTGGAAACCGTCCCCGTAGTCGG10.8130081300813009No Hit
CCCAAAAAGGGGAACACTATTCCATTTACAGAAGTAGAATTTTGGAACTT10.8130081300813009No Hit
AGCTTTAGCTTACAAAAGAGGCAGGAAGTTTAAATTCTTGTCATTTTCGC10.8130081300813009No Hit
GCCCATTAGCGACTGCGAGGCGAGCTTAGGTACCGGTGATCTGACGGGCC10.8130081300813009No Hit
ATGTTGTGCCTGATAGTTTCAACCACGGCACCTCGGCACAGCGCGCGGAA10.8130081300813009No Hit
GGCATCCTCTGCTATGGCGCGGGCACCTTCCTGCAGGCCATCGAGGGCGG10.8130081300813009No Hit
ATACTAAGCGATTTTTTTTCGTGTTATGATAGATTTCGGTTTCAGTTGAA10.8130081300813009No Hit
CTCCAAAGAAGACATCAGAAAATTGTTGAAGAGGCACCAGCCGTCTGTCT10.8130081300813009No Hit
GGCCAAGGGCTATCGTGGTCGCCGCAAGAACACCATCCGCGTCGCACGCC10.8130081300813009No Hit
AAAAACAGATTTTCGTCTTTCAGGACAGTGGATTCTTCCACTAAGTGATC10.8130081300813009No Hit
CTGTATACCCAAGCATTTCAGCCTGATCTTTTTCTGCTTCTGATATCCAT10.8130081300813009No Hit
ATATTGGCCGGGATCAAAAAGACCAGCGAGGCAATCAGCGCGGCCCAGCA10.8130081300813009No Hit
GGAAATTCGTAGTGAAAAACGGAAAGCATTGCGTGCGCTTCGTCCATTCT10.8130081300813009No Hit
CTCAGCTATCGGGTGCAAACCCCCTTCCACATTTTTAATTGCATTTCTGC10.8130081300813009No Hit
ATTCGCGGCGCACGATCACCACCGAAATACCGACCGGGCCCAGATTCTTC10.8130081300813009No Hit
GTAGAAATTCCATCAACAGCTGTTATCGCAGATCAATTTGCAAAAGAAGT10.8130081300813009No Hit
GTGTGATTACGTTCGTGCTGGTGCGATCGATCCCCGGCGACCCGGCGCGG10.8130081300813009No Hit
GTGGTGCTCAGGGTAACGCGCTTGACGTAAATGCCTTTCGAAGAAGCTGG10.8130081300813009No Hit
TCCCTAAGTACGCTGCGAGCGTCTTTTCAAACTTTGGAATATAAGGGCCT10.8130081300813009No Hit
AAATAAAGGAGGAGGCCGCAGGCCGGGGGACATTCGCGGAGCAAGGTACC10.8130081300813009No Hit
GGTATACAGGGCGTGTGATTCCCTGCAACAGGCCATTAGCAGGCTTTACC10.8130081300813009No Hit
GCGTTTCACCGCGCTGCAGTCGGGCGAGATCGACATTCTGTCGCGCAACA10.8130081300813009No Hit
CCTTTTTTACTCTTCGCCACATTTACAAATTTTCTGAAATAACTTCTAAT10.8130081300813009No Hit
CAAGGAATACCAACTGCTGCATGAACTGCTTTCGCCCCCGGGGCGGGTCA10.8130081300813009No Hit
TTGTTCGGCCAGGCCAAAGCCTATGCCCAGCGCCTGCTCGACGGACTGCA10.8130081300813009No Hit
GTCATCGTCATGGATGCAAAAGAGGTGCTTGGTGAGGGTTTTAAAAGGGC10.8130081300813009No Hit
TTCTGGTCAATACTTACCACAAGGGCCGAAGGTTGCGAGGCGCTCTTTAG10.8130081300813009No Hit
CGCCTGCGCCCTGGGCGCCGCCTGGCAAGCCAAGTACCACCGGCTTGCCG10.8130081300813009No Hit
AAGTTTGATTTTATTAATCAGTTTTAGGAAGAATCGAACAACAAAGAGAG10.8130081300813009No Hit
GTTTTGGACCAAATAATAACCGATATGAGGGGTAATTTCATTATGCATGC10.8130081300813009No Hit
GCGGAGCTGAGTTGGAATCATATGAGATGCCGCAATCACAGCTTGTTTGT10.8130081300813009No Hit
AGCTTATCCCTCATCTGTTAATCGAGAAAGTTACCATGAAACACGGCATT10.8130081300813009No Hit
GTCCTCGTCCATCAAGTACATCCAGTCGTCCATGCTCATTTCATAGGTCG10.8130081300813009No Hit
GTACATATTCATGCTGATCTAAATGGGCTAAAAAATCATCCAGCGTCACA10.8130081300813009No Hit
GAACTCTTTCACGGAAGAGACGAAGCGCGCGCGGATCGCGGAATCATGCA10.8130081300813009No Hit
CCAATGGAGTATCTGCTGGCATGGCGTATGGCGCTGGCAAAGACGCTGCT10.8130081300813009No Hit
TTTAAGACTTGTTTATCGAGGTAGAAACGAATATTTTCAGCAGGTGGTTC10.8130081300813009No Hit
CATTACAACCTCCTATAGGATCAATATGTACAACATGTAGGATTCTCACA10.8130081300813009No Hit
CTATCGTGTGCAGGTCCGCCGTGGTGATGCCTTCGGCCTCAGCCTTCGCG10.8130081300813009No Hit
AGTAAAGGCTGGGTTCCAATGGAGGATTAATGTCCTCAATCTGTCTCTTA10.8130081300813009No Hit
GTGAACGAGCAATTCCCCAAGCGATGCTTCGTTTATTGGCAACACCCATC10.8130081300813009No Hit
CTATTCGCACATGGTCGAGGCTGAGACCGGACAAGCGCCAGTGGCTGTGG10.8130081300813009No Hit
AACCAATCCACTGAGTCTGACTTTCGAGCTGCCCATGTCTGCCTTGCGCC10.8130081300813009No Hit
GGCAGAATGTGCATGATAAAAAATCAACAACAGATCTTAAACAATTTAAC10.8130081300813009No Hit
GTTGCAAGAAGCTGTCGATGCATTGCTCGACAACGGTCGTCGTGGTCGCG10.8130081300813009No Hit
AAGCCACCGAGCACGACCTGTTCGGCCTCGATGGAATGCGGCGGCACCTT10.8130081300813009No Hit
GTCGCGCTCAAGCGTGACCTGTCGCACAGCGATGCGCAGACCTATCTGCA10.8130081300813009No Hit
CAAGTAGTCCTCAAGCGGCAGACCAAGACCGCTGATACCCATCGCAAGGC10.8130081300813009No Hit
ATGAAAACACATATCAGCAAGACGAAGCTCATGTGGATCGTTATTTAAAA10.8130081300813009No Hit
ACCGCGGGCTGCGCATCGATCATCTCTTGCTCAACCGCACGTTGGCGGCG10.8130081300813009No Hit
TGACGGATGACACCACGCCAACCCTGCACGGTACGGGCCGTCCAGGTGAC10.8130081300813009No Hit
GGCAAGAGGTTACTCGAGAAATGACCGAGTAGGAAAAAGTTATTTAGAAT10.8130081300813009No Hit
GAATAACTGATGCCGCAGGTCACTTTTCTACCGCACGCCGAGCATTGCCC10.8130081300813009No Hit
GAAATGGCTTATGCAAGATTCCAGTGGTTTGCGAGCCTCGTCTTTACTGC10.8130081300813009No Hit
CTGGAATATCGAGGGTGACGATGCGGTTTTTGCCTGCGGCAGCCAGCATC10.8130081300813009No Hit
CTCAAGAGGAATCGGAAAAGTAACCGGCACATTCTATCAAGCTGGTCATG10.8130081300813009No Hit
CATAGAGGTAGTCGAGGCGGGTTTCGGCGGCGACCGCCTGGGCAAAATCG10.8130081300813009No Hit
CCATCGGGCTTTATCGATCAATGGGGAACGGGGTCTACAGATGCGAGCGG10.8130081300813009No Hit
TCATCGGGGCGGGCGCGGGGCTTGTCCAACCGAGGAAACCGGGCGCGGCG10.8130081300813009No Hit
GTAATGCGCCCTCTCACTGCTTTGTGTACCTATTATTACCGCCTGTCAGG10.8130081300813009No Hit
CTTATAGACCTTGCGCATCTGCTCGGCTTCGATCTCTTCGACCTTTTCCT10.8130081300813009No Hit
TCCCTGGACAGCGATGGCGGTGCAAGGCGCGCAGCACGCAAGACCTGTGG10.8130081300813009No Hit
CTCATATAGAAAAGGAACGGAGATGAGCATTAGTCCCGCGACCACCAAAA10.8130081300813009No Hit
CATACACGTGTGCTTGGCATTGTGCGCTACGGGAGCGGTTTGAACTGATG10.8130081300813009No Hit
CAGGATACGCATACTATAAAATTTCAAAAGCGTCGAATAAAGCGCAAGTA10.8130081300813009No Hit
GTGTTTTCAGGCGAATATAAGCTGTCAGACTGTGGAATCACCACAGGAGA10.8130081300813009No Hit
GACATACGATGCCGAGCCAAAAGCTCACTTTACCAAACAAAGTCATGCTT10.8130081300813009No Hit
CTTTCCACACGGTCTGGTACGGCGTCAGTCCTGGCGTTCGCTGTCGACTG10.8130081300813009No Hit
TCTGTGCGCCGAGGGCCGAGCTTAACGGGGTGATGTTCAGGCTGCTCATG10.8130081300813009No Hit
AACCTTGCGTTCGCTCATGGCACCTAGCTTCTAAAAAATAAAATCAAAGA10.8130081300813009No Hit
AGATATTTGGGACCAAATTCAGCAAGGCCATATTACAGGCTACTCAATGG10.8130081300813009No Hit
GATCAATAGCTTCAGCCATCAATACGATCGTGGCGGCAACCTCACGGTCA10.8130081300813009No Hit
GGCATCGACGAAGGACACACCGGTCAAAGGCGTGGACACCGGTACGGATG10.8130081300813009No Hit
GGGGGAACTGGTTTCTTGCGAAATTCTTGGGCAATGCCGGGAATCGGCCC10.8130081300813009No Hit
TCTTAAAAGAATCAGGAGCTGCAATCACTGAGCTTGCTGGTGTTGAGCCA10.8130081300813009No Hit
ATGTTCTACTTCTACGCAGTGTCCTTTGAGCCGTTCCTGATCCTTGCCCT10.8130081300813009No Hit
GGAATATATTGCGCCCGAGGCCCGCGCCCGCATCTGGTAAGGGCGCCGGA10.8130081300813009No Hit
CTTGTAATCTTCGAGCAATGGATATAGCGCTTCGTTCTTCGACTTGCAAG10.8130081300813009No Hit
GGCTACCGAAGAAGCCGCCCGCGCCGCGCAGATCGCGCTGATCGCCGAAG10.8130081300813009No Hit
GGCCGGAATGAGCGGTTTTGCGATCTCAAAGTCATCTTGGTCAATGTGAA10.8130081300813009No Hit
CAATACGCTGCGCAAGATCGTCCAGGAAGTTAACTCCGCCAAGGATCTCA10.8130081300813009No Hit
GCGATTATGGAAAAGGTTTTATGGCAGGCAAAAAAAAGCCCGCTGGGGAG10.8130081300813009No Hit
ATCCACGTCGATCTCGGAGCCGTCGAGGCGATCCTTCCTCCGGAGGAGCA10.8130081300813009No Hit
CATTGTTATCGTCGAAAGATGTCAAAGATTCTTGATGGGCGAAACTCTAC10.8130081300813009No Hit
GAGAAAGACAGCCGATCAACGGAGTTGGCAATATCATGAAAGCACGGGTC10.8130081300813009No Hit
GTACATAAGCAGCACCCGACTTGACGATCCCTAGAACTGAGACCATCATG10.8130081300813009No Hit
GCGCTCCAGCGACAAACCGCGACCGCCACCCTGATAGGCATAAGTCGAAT10.8130081300813009No Hit
GCGTACAATCGCGCACCCTGAACTTCCTGGGCAGCACCTGCCTTACGCAA10.8130081300813009No Hit
CTTCGGCACCATCGTGTCCGTTTGACACGGCGGCCCTCCTGATGGAACAA10.8130081300813009No Hit
TCTTCGGTACGTCCTTGTCTTGGCGATTCGGTATAAACAATTGCATTCAT10.8130081300813009No Hit
GACTGTCGCCTGCTGCCTGCATCAGCGGCAGCAGGCTTTCCATCAGGAAG10.8130081300813009No Hit
GGTCTTCGCCCTGACGTTCGGCCCGCTCGCTGGGGCTCGGGGTTCCTTCG10.8130081300813009No Hit
GATGAGGATTTCCTTGCAACAACGTCAGGCACAGCGCGGCATGGTCTTGC10.8130081300813009No Hit
CCTCAACGAGAAGGAGCGTGCGCCGGACGATGCACCGTCCATCGGGATCA10.8130081300813009No Hit
GTCTGATACTGATCGGGGAGTGCGCCGGCCTCATCGCTGGCAAGCCAGCT10.8130081300813009No Hit
GCCCCAGAAAACCGCCGATCAACTGCCAGCGCGGCAGTTCGGTCAGCGCC10.8130081300813009No Hit
GCTCGAGCACGCCGGCGTCAAGAAGGAGACCGTGGGCCTTGTTGTCCCCA10.8130081300813009No Hit
TCTCGGATCTGCCATAAGGCTGTAAAATCAGTCCAATGATCAAACATAAA10.8130081300813009No Hit
GGCCTCCCTGGCCTGGGCCGATGAAGCCAGCGACAAGCTGGACAACCCCA10.8130081300813009No Hit
ACATAGCCGTCGCGGATCATGAACAGGCCCGAGCCCACGAGACTATAGCG10.8130081300813009No Hit
CCCGTGTCCACCAGGATGCGGGCATCCGGGTGGTCGAGAATGTGCACGTT10.8130081300813009No Hit
TCAAATAAGGTCTCTTTTTTTTAAGGCTGCGGCTTCAGGTGATTCAGTGC10.8130081300813009No Hit
TCTCTGCCACGAGGATTTGGGAGGCAAGCGATGTATCTTTCACCAAACTA10.8130081300813009No Hit
CACCGGCAGGCGTCCCGGCCGCGCCGGTGCCCGCCGACAACCGCCCCGCC10.8130081300813009No Hit
GCAGTTTGCTGGAGTCGAGGTTGATCCCGTAGCCAAAGATAAAGAGCAGC10.8130081300813009No Hit
AACGAAACTTTGTTCGTTTTGTGTGAAAATCCATAGCATTGGTCGATTCC10.8130081300813009No Hit
ATCAACCTCGCCCCTGCTGAATGTGCGCTCGACCAGCACATTGCTGATCA10.8130081300813009No Hit
GGGACTGTTCCGCGTGCGCGGCCTTTCATTGGCCGCGGGCCGCTTCTTCG10.8130081300813009No Hit
TGCTTGGCGTCGGTGATGAGCGTCTTGATGACGTCGGTCGCCTTGGCCTG10.8130081300813009No Hit
TCGGTGAACGGCGCGCAAACCCGCATCATCAGCCGCAGCACCGCCAGCCG10.8130081300813009No Hit
CTGTAACACCGGGGATGTCAGCGACGATTTGCTGCCAGGCTGGCGGTTGC10.8130081300813009No Hit
GTTCAATTGATAGAAAAGCTGTTCAAGTGTGACATTGTACCAGCCTGCAA10.8130081300813009No Hit
ACTTTATCTTCAATAATGGCTGCATCAAAACGGCCTGCTTTAATTTCTTG10.8130081300813009No Hit
AGCAACCAGTGCGAAAGCAGATAGCCCAGCACAATCAGGCCGCCGGTCAG10.8130081300813009No Hit
GTACGACTTTGTTGTCACGTAGGGACGAAGTGGCATCGGAGGTCGCGTCG10.8130081300813009No Hit
CCTACAATTCCAATCGTCTGACCAATTGGCTTTGGCAGTCGCACTCCTGC10.8130081300813009No Hit
GACCAGCGCAAGGCCGAGATGAGCGAGTACCTGGCGCTGTTCGAGCAACT10.8130081300813009No Hit
ATGTAGAACAGGATGCCGAACAGGGTGAAGGCGTCGCGCAGTGTCGAATA10.8130081300813009No Hit
CCCGACATTGGCCCCTATATGCACCGTTTGCGCGAGCTGTCCGACGAGCC10.8130081300813009No Hit
CAGTGCCAGAATGATCAGCCCCAGGCCGATGCCCACCCGACCCAACTGCC10.8130081300813009No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)