FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n01_Tr4_C15I1b_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n01_Tr4_C15I1b_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76
Sequences flagged as poor quality0
Sequence length251
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAATAATGACTACCGACACGACCCCCGCGGGTCCGGGAGAATCCTTGA11.3157894736842104No Hit
TACAAAACTGAGGAGGGAAAACGATGAATGAAGTTCTAGAAAAAGCAAAA11.3157894736842104No Hit
TCATAAAATAGTAGACGACCGTTTACCCGATGAAGATTATTTATTTGACG11.3157894736842104No Hit
CGACCGTACTGCGTCACGCCGACAATCAGGATGTCGGCGTGACGCAGTAC11.3157894736842104No Hit
GAACCCGTCTCCTCCAGCAACGGTGCGGCCTCGCTGACCTTCACTAGATC11.3157894736842104No Hit
CACAAAAAGCCCTGGCCGAGCAAGTAAAACAGCAACAGCTTGTGGCACTG11.3157894736842104No Hit
AATCAGGTACACATATAAAAAAAGACCCAATGATACGTTGGGGCTTTTTG11.3157894736842104No Hit
CTGCATGCCTTCTACAACCGACAGTTCGTTTTCCAGGCCCGAGCCTTCTT11.3157894736842104No Hit
CATCTGCATATGGATCACGTTGGTGGGCTGCTTATTGAGGGGGTGAAAGC11.3157894736842104No Hit
GACTTGAATTCCCTGTTGTGTTCTTAATGGGAATGGAAGAAGGCGTCTTC11.3157894736842104No Hit
CTATGTTGACTTAAAGTTCACTTTAAGACGTACAGTATAGAGGAAGGACC11.3157894736842104No Hit
GTAGACACCATGAGGTCTTCTGTATTCATGGCTGTATTCTTGAATCTGTC11.3157894736842104No Hit
GTCATAACGGAAAAGCCGATGGTCACAACAGCAAAAGACGCCAAACAGGT11.3157894736842104No Hit
GCCTTGATGCGTCGCGTCCTGTTTGCATTGTTTCTGCTGATCGCGCTGCC11.3157894736842104No Hit
ATACAAGGCCACCGGCGCACCGAGCCCACGAGACGTCTGCTAATCTCGTA11.3157894736842104No Hit
GCATCAATGAGGTAGCGCTCATGGTGATGTTGAAGGACGATGGCTACATG11.3157894736842104No Hit
AGGACGTCGTGGGTCTCGGGCAGGTTTTCGTTGTTGGTGCCGTCGCGCTC11.3157894736842104No Hit
AGCAAATAGTTGGAGAAACGGAACACCATTTGATACTAAAAAAGCGTCTC11.3157894736842104No Hit
ACTATATCTACGGGTACCCAGGTGGTGCTCTTCTGCATGTTTACGACGCG11.3157894736842104No Hit
CACGTACAACAGGTAATCCAAAAGGCTGTATGGTCGGACATGGACGAATC11.3157894736842104No Hit
CAGCTGGAACTGCGGCGCCGAAGGTCCCACCGACGACCCCCAGATCCTTG11.3157894736842104No Hit
CCTCATGCCCGCGCGCTACGCTATCGCGATCGCCTTGCAGCGCTCAGGTA11.3157894736842104No Hit
TCTTGAAACAAGTCAGCCACACATTTATGCCATAGGCGACTTAGTGGGTG11.3157894736842104No Hit
CATACGACGTGATGGCCTCCAAGCACCTGCGCGGCGACGTCAACCCTGTC11.3157894736842104No Hit
CCGGGAAGTCCTCTCCAAAGTTGACGCTATTAAATCCGTGGCGGGTGAAC11.3157894736842104No Hit
CTCGAGGAACTGCTCGCGCCCACCGACGTGTTCGTGATCAGCGACGAGGT11.3157894736842104No Hit
ATCACCATGTGCCGCAGTTCGCCTACTGGGGCATGCAGGCCAAACTGGCT11.3157894736842104No Hit
TCTCTGAGATATTCCAAAAAATTGGAAGCAAAAAGAAAAGTAAACGTGTC11.3157894736842104No Hit
GTCATATTCAGCCTCGGCCGCGACGACCAAAGAAGAACGAAGCAATGAAC11.3157894736842104No Hit
CAGTGAGCACACTCGTCTGCAATTCCGTCCGCACGTCCCGTTTTTCTATC11.3157894736842104No Hit
GCCATCCACCGAGGCCTGGCACTCGAACTTCCAGATCTGGCGCTTGCAGC11.3157894736842104No Hit
GTTTCAATCCCGTGGCCTGCAGGTTCAACGCCAATCAGCCCGACGCTGGT11.3157894736842104No Hit
GGCCCAGCAATACCGCCAGCGCCAGGCCGGTGTAGGTGGTGTCCAGGCCG11.3157894736842104No Hit
ATCCACAAAGCCGTACGCGCCCGCCTCGGCACCCTCGTCAGCCTGTCCGC11.3157894736842104No Hit
GTTCGGTCTCCACGAGGTGCCGGCCGGCTTCCAGCTCACCGCTGGTGATG11.3157894736842104No Hit
TACTCGCTGTGGTCGGCCATCGGTTTGCCGATCGCCCTGGCCATCGGCAT11.3157894736842104No Hit
ACCTTCTACATGCCGACCTACGCGGTGCGCGAGCTGGGGCTCGCCCCATC11.3157894736842104No Hit
CCATTCGATGGGCGATGTGACAAAAGATTTAATTTTTAAAGGCGTGACAA11.3157894736842104No Hit
AGAGTTAGCTGCTCTACCAACTGAGCTATGCGTCCCCACGAGTGCCCGCA11.3157894736842104No Hit
CCTGTACATACTGTTTTGTCAAATAGCGGCCAAGAGCGGAGTAACTTCTC11.3157894736842104No Hit
AGTGTACTGTTTCCCTAGCTACAACCAGTAGTTTTGCTAAGGTTAAGCGG11.3157894736842104No Hit
GTAATAACCATAGCATAACAGAGACGCGAAAAGTGTGACATGGGATAAAA11.3157894736842104No Hit
TCGATGTCCTTGACGATACCGCGCACCGGCGAACGCAGGGTCAGGCGCGA11.3157894736842104No Hit
GCGTTTCGACTCGGTCAGCCGCTCCGCGATCGCTCAGCGCCAGGTGAACG11.3157894736842104No Hit
TGTGTGGTTTGAGGTCGTAGAAATCGCTGCCGAACCATTGGCCGGTACGG11.3157894736842104No Hit
TGATATATGTCAAGTAAATTACAATAATAGTTTACATATAGAGTGTAAAA11.3157894736842104No Hit
CGCCAGCCAGGACGCTCCGCGTCCGCGCTCGAATGTGGCGCAGAATGAAG11.3157894736842104No Hit
CATATAAACCGCACCGGCGTTGATGCGCTCGCTGATGCTGGCACGGGTAA11.3157894736842104No Hit
CCGTACTGCTGCTTTTTGTAGGATTGGCCATCGCCATTTTGTTCATGGGC11.3157894736842104No Hit
ACATGGAACTCTGGAGTTCTGAAATGAATGGCCCCAATGAAATCAAAGTG11.3157894736842104No Hit
GTACCAATGTTCCAAGAGATGGAGAAGCTTGCAGAAATTCTGCGTGACAA11.3157894736842104No Hit
AATTATGGCTGCTTACTTTCCTTACTATCGCATTGTTAGCATTCGTCACA11.3157894736842104No Hit
GATTCAATGATATGGATGAAAACAGCTTAGTGCATCAGACTGAGCTGTTT11.3157894736842104No Hit
TGATAGACGTTATGCGATTGATCCAACAAAAATAGAAAAAGAACTCCAAT11.3157894736842104No Hit
ATCAAGGTCTACACCATCCTCAACATGTCCGGCCAGCTCGACGGCAAGGA11.3157894736842104No Hit
TCCTATGTCGAATGCTACTAAGAGTAAATTTCAATCCTTGGATAGAAATC11.3157894736842104No Hit
TGGTTGCCGTCAGGACCACCAGAACGAGAAGCGTCATCATAACCGTGCCG11.3157894736842104No Hit
CAATAATGCACCGTTGTAAATGGCGGAAACATTTACTCAATGTGACAGTC11.3157894736842104No Hit
CTTCATAGGCCACCCCGGCAGCTTCCTTGACCTCTGACTCCCTCAAGACC11.3157894736842104No Hit
TGCCTTATGTGCTGAAGATGGTCGAAGGCGTGAAGGCCATGGGCCTGTAA11.3157894736842104No Hit
GCAAATCATAATCGGATGTATTTTATTAGCAGCAGGTATCGGGCTTGTAC11.3157894736842104No Hit
ACCTGCAACCTGGCCCCGTCAACCTGGAAAACGGCTATTTCGGGCGCATG11.3157894736842104No Hit
TAATAAAAGAGCAGGCTGAATATACCGGTGCAGCTAAAATTAGCACCAGT11.3157894736842104No Hit
ATTTCGTGCAGCAGCTTGAGCAGCGCGGAGAGTTGAAACGCCTGTCTCTT11.3157894736842104No Hit
AATCAACAATATCCGCCAATAGCGGACATTGCCGACTTCGCCCCCTATTC11.3157894736842104No Hit
ATCTACACCTCCGGCTCCACCGGCCTGCCCAAGGGCGTGGCGGTGGCGCA11.3157894736842104No Hit
TCTTCGATCTGCGCGTTGGCGGTAAAACCGAGAATCAGGCAGCGCCTGCG11.3157894736842104No Hit
ACTGCAGGCCTTCTGGGTGCTGCGGCGAGCCATGGTTGGCTCGTCCCCGA11.3157894736842104No Hit
ACAATGAGAAGCGTATCAACATATCGCTGGGATCGCTCAGCCCGGTTGAG11.3157894736842104No Hit
CTCCGAGACCATCGGCGGCGTCGCGCCGCGGTGGTCGGGCGAGGGCAGGA11.3157894736842104No Hit
GCGAAACGCAGCCCACCGTGGCAATGCCGCCGCCGATGCCGATGCCGCGC11.3157894736842104No Hit
GTCCGATACACATGATCAACTAATTGAAAAATCCGTTTGATTTCAGCTTC11.3157894736842104No Hit
GAACATGCTGTTCGATCTGGTGGCCCGAGAGCCGGTTTTGAGTTGTCGCG11.3157894736842104No Hit
GTACCATCACCAGCGACGACTGCACACTCATCATCGAACGATAGCCGGAA11.3157894736842104No Hit
CAATCGTAGCTGTAGCACCAAGCACTCCGCCTCGATGGTCATAGACGAGT11.3157894736842104No Hit
GCAATATACGAACCGCTTCATGATGTCTTGCTTGCACTGGGTCCGAGGCC11.3157894736842104No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)